Cycling binding protein cell cycle. Medical search. Frequent questions

Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
The accumulation of both Cyclin and CKI proteins is tightly regulated at the level of transcription. (intechopen.com)
In addition, Cyclin and CKI proteins are controlled at the level of their destruction. (intechopen.com)

It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. (novusbio.com)
miR-203 drives breast cancer cell differentiation. (medscape.com)

During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)

Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)

The late entry into the S/M phase coincides with the timing for the peak expression of PF13_0027, suggesting that the deficiency in the mutant cycle can be correlated with the gene expression pattern ( 26 ). (frontiersin.org)

These results suggest a strong link between ovulatory menstrual cycles and the risk of developing breast cancer, with progesterone playing an important role. (medscape.com)

Macrophages are essential inflammatory cells which regulate the features of immune reactions within tumors. (cancerindex.org)

What pathways are this gene/protein implicaed in? (cancerindex.org)

Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
Tumor-infiltrated macrophages were isolated from human GC tissues using magnetic beads, gene transcription was determined by real-time PCR, protein expression was monitored using western blots, metabolites were determined using HPLC, and transcriptional regulation was analyzed by the luciferase-based reporter gene system. (cancerindex.org)

The reduction in the residual innate immune effectors (mainly cells of monocytes and macrophages lineage) and co-administration of huRBCs supplied with decomplemented human serum through an intravenous route led to the development of a reproducible humanized mouse. (frontiersin.org)

SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)

We show that loss of the DNA repair protein XPA markedly augments the synthetic lethality between MK2 and p53, enhancing anti-tumor responses alone and in combination with cisplatin chemotherapy. (nature.com)
Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)

These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)

ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Albumin, which exists in far higher concentrations than SHBG, also binds sexual steroids - although with a clearly lower binding affinity (e.g. about 100 times lower for testosterone). (cdc.gov)

In response to DNA damage, a synthetic lethal relationship exists between the cell cycle checkpoint kinase MK2 and the tumor suppressor p53. (nature.com)
Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)

Breast metastatic tumors in lung can be substituted by lung-derived malignant cells transformed by alternative splicing H19 lncRNA. (medscape.com)

This data will allow for population estimates of the selected steroid hormones and related binding protein that can be used to assist in disease diagnosis, treatment, and prevention of diseases such as, Polycystic Ovary Syndrome (PCOS), androgen deficiency, cancer, and hormone imbalances in children. (cdc.gov)

In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)

Controlled reactive professional phagocytic leukocytes in immunodeficient mice allowed for sizeable human blood chimerism and injected huRBCs acted as bona fide host cells for P. falciparum . (frontiersin.org)
This NSG mouse, depleted with γ-chain of the IL-2 receptor, has been shown to better tolerate a variety of human transplanted cells ( 17 - 24 ). (frontiersin.org)

The small protein ubiquitin plays a vital role in virtually all aspects of cellular life. (intechopen.com)

Therefore, C9-M and C9-C (Rescued phenotype of wild-type growth by genetic complementation) parasites ( 26 ) showed the attenuation in PF13_0027 knock-out parasites (C9-M) growth, which in turn resulted in the irregular cell cycle. (frontiersin.org)

The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. (genetex.com)

and hypermethylation of the p53 (tumor suppressor protein p53) gene ( Mass and Wang 1997 ). (nih.gov)
Potential applications for live-cell studies are in the determination of cellular DNA content and cell cycle distribution for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms. (enzolifesciences.com)

In conclusion, these results indicate that Manu A is a potential to treat human OSCC via cell apoptosis through the downregulation of Sp1. (spandidos-publications.com)
Many studies have demonstrated that Manu A inhibits cell viability and induces cell apoptosis in many cancers, such as prostate cancer, multiple myeloma, anaplastic thyroid cancer and colon cancer ( 14 - 17 ). (spandidos-publications.com)
To induce apoptosis of cancer cells by targeting the specific signal-transduction pathway could be an effective anticancer therapy. (spandidos-publications.com)
Therefore, we investigated whether the Manu A-induced cell apoptosis is related to Specificity protein 1 (Sp1), a transcription factor that binds to a specific DNA sequence, overexpressed in many cancer cells, such as bladder cancer ( 19 ), breast cancer ( 20 , 21 ), pancreatic cancer ( 22 ), gastric cancer ( 23 ) and oral cancer ( 24 ). (spandidos-publications.com)
Specificity protein 1 (Sp1) has already been examined and plays important physiological roles such as cell cycle regulation, cell proliferation, and cell apoptosis ( 25 ). (spandidos-publications.com)
The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. (genetex.com)
Based on functional enrichment analyses, up-regulated polyploidy genes appeared to implicate several functions, which primarily include cell/nuclear division, ATP binding, metabolic process, and mitochondrial activity, whereas that of down-regulated genes primarily included apoptosis and immune processes. (plos.org)

Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
During a replicative cycle, CRK5 stably interacts with a single Plasmodium -specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. (elifesciences.org)

ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
We confirmed that combining eIF4A3 with H19 obstructed the recruitment of eIF4A3 to the cell-cycle gene mRNA. (sciencepop.org)

HeLa cells were grown to ~60% confluency. (enzolifesciences.com)
WB analysis of HeLa cell lysate using GTX34934 p53 antibody [TRP/816]. (genetex.com)

Oral squamous cell carcinoma (OSCC) which occurs in the lining of the epithelial cell represents approximately 95% of head and neck cancer and is the sixth most common malignant neoplasm worldwide ( 3 - 5 ). (spandidos-publications.com)
The pesticide DDT and the food colorant Red No. 3 were found to increase the growth of HTB 133 but not estrogen receptor (ER) negative human breast cells (HTB 125) or rat liver epithelial cells (RLE). (researchgate.net)
the comet assay in peripheral blood-isolated lymphocytes and mammary epithelial cells was also carried out. (researchgate.net)
Our results suggest that DDT is genotoxic, not only for lymphocytes but also to mammary epithelial cells. (researchgate.net)

Enzo Life Sciences' GFP-CERTIFIED ® NUCLEAR-ID ® Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. (enzolifesciences.com)
Figure 2: Live cells treated with different drugs show inhibition of cell cycle progression at different phases. (enzolifesciences.com)
The inhibition of mitochondrial activity by various pharmacological inhibitors, as well as by gene-specific targeting using siRNA-mediated technology showed a dramatic attenuation of polyploidy and bi-nucleation development during in vitro stromal cell decidualization, suggesting mitochondria play a major role in positive regulation of decidual cell polyploidization. (plos.org)

Figure 1: Three-dimensional reconstruction of the spatial relationship between the green fluorescent protein-expressing (GFP-expressing) mitochondria and the nucleus using a structured illumination method, as implemented with the ApoTome from Carl Zeiss, Inc. This imaging method enabled creation of optical sections through the nucleus using a conventional fluorescence microscope, for improved resolution along the optical axis. (enzolifesciences.com)

Expression analysis showed that exposure of cells to DMA III resulted in a gene profile different from that in cells exposed to iAs III or MMA III , and that the iAs III -induced gene profile was closest to that in the tumorigenic HUC-1-derived 3-methylcholanthrene-induced tumorigenic cell line MC-SV-HUC T2, which was derived from SV-HUC-1 cells by methylcholanthrene treatment. (nih.gov)
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. (genetex.com)

Progression through the cell cycle critically relies upon post-translational mechanisms including changes in activity of cell cycle kinases and phosphatases, and ubiquitin-mediated degradation of specific components once their function is complete. (elifesciences.org)

Site-specific DNA binding by p53 in RLE, HTB 125, HTB 133, and MCF-7 cells was increased when they were treated with Red No. 3, which suggests that cellular DNA was damaged by this colorant. (researchgate.net)

Cells were stained with NUCLEAR-ID ® Red DNA Stain or a competitor's dye at a final concentration of 2.5 or 5µM for 15 min at 37°C and gently washed post-staining. (enzolifesciences.com)
At 24h, the competitor's dye intensity and cell growth were dramatically reduced at the 5µM final concentration. (enzolifesciences.com)
The NUCLEAR-ID ® Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs. (enzolifesciences.com)
Dilutions can vary depending on cell strain and cell concentration. (enzolifesciences.com)

For injectable suspension: white to yellow, sterile, lyophilized powder containing 100 mg of paclitaxel formulated as albumin-bound particles in single-dose vial for reconstitution. (nih.gov)

Recommended dosage of Paclitaxel Protein-Bound Particles for Injectable Suspension (Albumin-Bound) is 260 mg/m 2 intravenously over 30 minutes every 3 weeks. (nih.gov)

Avoid freeze/thaw cycles. (enzolifesciences.com)

In the receptive uterus on day 4 of pregnancy (day 1 = vaginal plug), the onset of embryo implantation elicits the prerequisite transformation of stromal cells into decidual cells (decidualization), which is a gateway to pregnancy establishment. (plos.org)

Cancer cells tend to form new cells more quickly than normal cells and this makes them a better target for chemotherapy drugs. (cancer.org)

Consistently, studies revealed a marked induction of mitochondrial mass and ATP production in polyploid cells. (plos.org)

The kit is suitable for (1) determining the percentage of cells in a given sample that are in G 0 /G 1 , S and G 2 /M phases, as well as to quantify cells in the sub-G 1 phase, and (2) DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. (enzolifesciences.com)
Suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. (enzolifesciences.com)
We observed that Manu A reduced cell growth and Sp1 protein levels in OSCC cell lines (HN22 and HSC4) in a dose- and time-dependent manner. (spandidos-publications.com)
Table 2 Univariate and multivariate Cox regression analysis H19 for DFS or OS of patients in study cohort (= 83) Manipulation of H19 levels in CRC cells To evaluate the biological functions of H19 we next performed qRT-PCR analysis to examine the expression levels of H19 in a variety of cell lines including HCT116 HT29 SW480 Lovo and the normal colon epithelium cell collection CCD-18Co. (sciencepop.org)

Collectively, analyses of unique polyploidy markers and molecular signaling networks may be useful to further characterize functional aspects of decidual cell polyploidy at the site of implantation. (plos.org)

These highlights do not include all the information needed to use PACLITAXEL PROTEIN-BOUND PARTICLES FOR INJECTABLE SUSPENSION (ALBUMIN-BOUND) safely and effectively. (nih.gov)
Do not substitute Paclitaxel Protein-Bound Particles for Injectable Suspension (Albumin-Bound) for other paclitaxel products. (nih.gov)
administer carboplatin on Day 1 of each 21-day cycle immediately after Paclitaxel Protein-Bound Particles for Injectable Suspension (Albumin-Bound). (nih.gov)
Severe hypersensitivity reactions to Paclitaxel Protein-Bound Particles for Injectable Suspension (Albumin-Bound). (nih.gov)

These drugs work in all phases of the cell cycle and are used to treat many different cancers, including cancers of the lung, breast, and ovary as well as leukemia, lymphoma, Hodgkin disease, multiple myeloma, and sarcoma. (cancer.org)

Our data demonstrate that chronic exposure to iAs III , MMA III , or DMA III has different epigenetic effects on urothelial cells and represses NF-κB activity. (nih.gov)
It has been known for several years that exposure to some xenohormones increases the production of reactive oxygen species, which in turn could inflict structural damage to cell DNA of target organs, as well as to DNA from other systemic cells [4, 5]. (researchgate.net)
On the other hand, it has been reported that exposure to some xenoestrogens and to estrogenic metabolites promotes the production of free radicals through the hydrogen peroxide (H 2 O 2 ) reduction cycle, by means of the P 450 oxidase and reductase enzymes, giving origin to the production of oxygen reactive species which might damage the genetic material [33]. (researchgate.net)

This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
In normal control adult mouse eyes, ETS-1 was mostly present in the nuclei of all neuroretinal layers whereas ETS-2 was mostly localized in the cytosol of the cell bodies of these layers with a smaller amount present in the nuclei. (molvis.org)
Each time chemo is given, it means trying to find a balance between killing the cancer cells (in order to cure or control the disease) and sparing the normal cells (to lessen side effects). (cancer.org)

However, the anticancer effects of Manu A on oral squamous cell carcinoma (OSCC) have not been reported. (spandidos-publications.com)

Locally advanced or metastatic non-small cell lung cancer (NSCLC), as first-line treatment in combination with carboplatin, in patients who are not candidates for curative surgery or radiation therapy. (nih.gov)
However, the relationship between Manu A treatment and downregulation of Sp1 in OSCC cells has not been studied yet. (spandidos-publications.com)

1.800.227.2345 ____________________________________________________________________________________ between healthy cells and cancer cells. (cancer.org)
This means normal cells are damaged along with the cancer cells, and this causes side effects. (cancer.org)
But cancer cells are mutated (not normal) cells, and they usually do not recover from the effects of chemo. (cancer.org)
This is why chemo is good at killing many types of cancer cells. (cancer.org)

Chemotherapy drugs target cells at different phases of the cell cycle. (cancer.org)

A sample from each lot of GFP-CERTIFIED ® NUCLEAR-ID ® Red Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. (enzolifesciences.com)

Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. (elifesciences.org)

Polyploidy has been widely reported to occur in a large variety of plant and animal cells. (plos.org)

Every time any new cell is formed, it goes through a usual process to become a fully functioning (or mature) cell. (cancer.org)
The process involves a series of phases and is called the cell cycle . (cancer.org)

The good news is that most normal cells will recover from the effects of chemo over time. (cancer.org)

Because these drugs damage DNA, they can affect the cells of the bone marrow which make new blood cells. (cancer.org)

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. (genetex.com)

The present study evaluated genotoxicity produced by the pesticide DDT on systemic and mammary gland cells obtained from adult female Wistar rats. (researchgate.net)
In the present study, to better define the aspects of decidual cell polyploidy, we isolated pure polyploid and non-polyploid decidual cell populations from the in vivo decidual bed. (plos.org)

Anatomy22

Organisms12

Diseases3

Chemicals and Drugs119

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes75

Disciplines and Occupations1

Information Science3

Health Care2

Cyclin3

Differentiation2

Proliferation3

Inhibitor1

Gene expression1

Breast Cancer1

Regulate1

Pathways1

Regulation3

Macrophages1

Genes2

Chemotherapy2

Mechanism1

Higher2

Damage2

Alternative1

Disease1

Essential1

Human2

Role1

Growth1

Activity1

Transcriptional activation1

Tumor2

Apoptosis7

Transcription2

MRNA2

HeLa2

Epithelial4

Inhibition3

Mitochondria1

Gene2

Progression1

Cellular1

Concentration4

Vial1

Dosage1

Avoid1

Transformation1

Tend1

Mass1

Levels4

Functional1

Suspension4

Cancers1

Exposure3

Control3

Oral1

Treatment2

Cancer cells4

Target1

Analysis1

Unique1

Variety1

Process2

Effects1

Affect1

Signal1

Study2