Rabbit polyclonalProtein kinaseCDK2TumorProteinsAntigenUpregulationCdk6CDK3GeneInteractsRegulatorsRetinoblastomaArrestInhibitsDistinctExpressionCellsProgressionHumanLevelsBreast CancerCellPolyclonalSubstratesAntibodiesKinase activityDegradationKinasesCdk1ProteinsAlterationsOvarianCellularPrognosisActivityInteractExpression level
Rabbit polyclonal1
- Our Cyclin E1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. (phosphosolutions.com)
Protein kinase1
- CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. (mybio.ie)
CDK22
- This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. (wikipedia.org)
- Cyclin E1 appeared to be the most significant predictive indicator of how a patient will respond to palbociclib, and they identified possible others including associated protein CDK2. (reachmd.com)
Tumor4
- Cyclin E1 expression is elevated in human tumor cells. (phosphosolutions.com)
- Analysing levels of a molecule called cyclin E1 in tumor biopsies could be used to guide breast cancer treatment-helping delay chemotherapy for some women and ensuring others are closely monitored for any signs of progression. (reachmd.com)
- In the PALOMA-3 trial, women with low levels of cyclin E1 responded to combination treatment for an average of 14.1 months before their tumor started growing again, compared with only 7.6 months in women with high levels, and 4 months among those who received a placebo plus hormone therapy. (reachmd.com)
- In the POP trial, palbociclib treatment stopped the tumor growing in 80 percent of women if low levels of cyclin E1 were present, whereas palbociclib only stopped the tumor growing in 36 percent of women it high levels of cyclin E1 were present. (reachmd.com)
Proteins2
- Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. (mybio.ie)
- Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
Antigen1
- It detects human Cyclin E1 and is antigen affinity purified. (phosphosolutions.com)
Upregulation2
- hBMMSCs exposed to 37, 45, and 55C for 120 s demonstrated significant upregulation of BAX, P53, Cyclin A2, Cyclin E1, TNF-, and HSP70 in cell suspensions compared to cell pellets. (mingsheng88.org)
- More cells entered S phase upon treatment of EGCG, accompanied with upregulation of cyclin D1 and cyclin E1. (hairloss-research.org)
Cdk61
- 1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
CDK33
Gene3
- G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. (wikipedia.org)
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (wikipedia.org)
- The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E". Genes Dev. (wikipedia.org)
Interacts2
Regulators1
- Cyclins function as regulators of CDK. (wikipedia.org)
Retinoblastoma1
- Lundberg, A. S. & Weinberg, R. A. Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes. (nature.com)
Arrest1
- Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
Inhibits3
- Concurrent deletion of cyclin E1 and cyclin-dependent kinase 2 in hepatocytes inhibits DNA replication and liver regeneration in mice. (nature.com)
- Previous studies have shown that by inhibiting histone H3 deacetylation round the let-7f-2/miR-98 promoter, HDAC10 suppresses HMGA2 expression which target to CP-640186 cyclin A2 promoter, and further inhibits the transcription of cyclin A2 [30,36]. (geogise.com)
- The signaling pathways: HDAC10-let-7f-2/miR-98-HMGA2-cyclin A2 arrests the G2/M transition and finally inhibits lung malignancy cell proliferation. (geogise.com)
Distinct1
- Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (wikipedia.org)
Expression2
- These accumulations were associated with decreased levels of Cyclin A1, B1, and E1, and increased expression of p16 and p27. (elsevierpure.com)
- HDAC10 plays an oncogenic role by inhibiting the expression of P27, P21 and enhancing that of cyclins D1 and E1 [29] (Physique 1A). (geogise.com)
Cells3
- Western Blot of transfected 293T cells showing specific immunolabeling of Cyclin E1. (phosphosolutions.com)
- Cyclin E1 is also involved in the checkpoints that dictate when a cell can multiply, and the researchers hypothesize that high levels of cyclin E1 allow cells to bypass other checks and keep multiplying, uncontrolled. (reachmd.com)
- They also found that targeting MEK1 and Cyclin E1 with small interfering RNA decreased the number of endothelial cells. (diki.pl)
Progression2
- In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)
- Evidence suggests that both cell cycle inhibitors (such as P21 and P27) and promoters (such as cyclins E1 and D1) play a vital role in malignancy progression [37C39]. (geogise.com)
Human1
- For instance, overexpression of cyclins or the elimination of CDK inhibitors or pRB due to genetic mutation are common cause in human cancer. (ukessays.com)
Levels2
- The study by the team at the Institute of Cancer Research, London, and The Royal Marsden NHS Foundation Trust, showed that women with low levels of cyclin E1 in their tumors responded to palbociclib given in combination with hormone treatment for nearly twice as long as women with high levels. (reachmd.com)
- But the researchers suggest that women with higher levels of cyclin E1 in their tumors should be monitored especially closely, since they can be expected to stop responding much more quickly than others. (reachmd.com)
Breast Cancer1
- Researchers found that testing for cyclin E1 could predict the chances of a year or more of successful treatment with a drug called palbociclib-a drug hailed by doctors as the biggest advance in breast cancer treatment for 20 years. (reachmd.com)
Cell1
- The cell cycle is a tightly regulated process that is controlled by the conserved cyclin-dependent kinase (CDK)-cyclin protein complex 1 . (nature.com)
Polyclonal1
- Find available monoclonal or polyclonal Cyclin E1 Antibodies. (antibodies-online.com)
Substrates3
- FBXW7 substrates, Cyclin E1, MCL-1 and c-MYC, are putatively amplified in about 20% of serous ECs, indicating that their de-regulation may be important in these tumors. (nih.gov)
- Ubiquitin and ubiquitin-like proteins control the degradation of substrates as diverse as cyclins, viral envelope proteins, plasma membrane receptors, and mRNAs. (cdc.gov)
- It is attached covalently to its substrates following activation by the UBIQUITIN-ACTIVATING ENZYME E1-UBA3 enzyme complex. (nih.gov)
Antibodies8
- Your search for reliable Cyclin E1 antibodies ends here. (antibodies-online.com)
- Utilize our Cyclin E1 antibodies in your research endeavors for dependable Cyclin E1 detection. (antibodies-online.com)
- Find Cyclin E1 Antibodies for a variety of species such as anti-Human Cyclin E1, anti-Mouse Cyclin E1, anti-Rat Cyclin E1. (antibodies-online.com)
- Find Cyclin E1 Antibodies validated for a specific application such as WB, IHC, FACS, ELISA. (antibodies-online.com)
- Find Cyclin E1 Antibodies with a specific Host. (antibodies-online.com)
- Find Cyclin E1 Antibodies with a specific conjugate such as Biotin, FITC, Alexa Fluor 488. (antibodies-online.com)
- Cyclin E1 antibodies (mAbs E12 and E172) were obtained from E. Harlow. (medscape.com)
- C) GST-Cdc25A proteins (phosphorylated or not by Chk1) and egg extracts (equivalent to 10 eggs) were both prepared as in (B) (GST-Cdc25A proteins being eluted from glutathione beads), mixed together, and incubated for 1 h at 4°C. GST-Cdc25A proteins were then immunoprecipitated with anti-Cdc25A antibody (IP) and analyzed by immunoblotting using anti-GST or anti-cyclin A1 antibodies. (bioz.com)
Kinase activity1
- [ 45 ] Cyclin E1-dependent kinase activity was assayed and quantified as described. (medscape.com)
Degradation3
- Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (wikipedia.org)
- To determine whether FBXW7 mutations impair the degradation of Cyclin E1, MCL-1 and c-MYC in EC, we measured their stability in FBXW7 mutant and wild-type EC cell lines. (nih.gov)
- Stimulation of hERG1 channel activity promotes a calcium-dependent degradation of cyclin E2, but not cyclin E1, in breast cancer cells[J]. Oncotarget, 2015, 6(3): 1631-1639. (sdu.edu.cn)
Kinases1
- The earliest stages of animal development are largely controlled by changes in protein phosphorylation mediated by signaling pathways and cyclin-dependent kinases. (xenbase.org)
Cdk11
Proteins1
- Cyclin E1 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research. (rockland.com)
Alterations1
- The most frequent alterations were in loci harboring candidate oncogenes: cyclin E1 (CCNE1), AKT2, Notch3 and PIK3CA as well as in novel loci, including 12p13, 8q24, 12p13 and 12q15. (johnshopkins.edu)
Ovarian2
- 13. The concurrent expression of p27(kip1) and cyclin D1 in epithelial ovarian tumors. (nih.gov)
- The preclinical research of ATRN-1051 in ovarian cancer also shows an increased expression of cyclin E1, or CCNE1. (healthtechnologynet.com)
Cellular1
- Negatively regulates cyclin E1 promoter activity and cellular proliferation. (nih.gov)
Prognosis1
- Objective This study aims to evaluate the level of expression of cyclin E2 in hypopharyngeal cancer and its relationship with clinical characteristics as well as prognosis. (sdu.edu.cn)
Activity2
- Cyclin E1 activity is required for centrosome duplication during S phase and this mechanism could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis (1). (rockland.com)
- 10. p27 Kip1 nuclear localization and cyclin-dependent kinase inhibitory activity are regulated by glycogen synthase kinase-3 in human colon cancer cells. (nih.gov)
Interact1
- This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. (nih.gov)
Expression level1
- The relationship between the expression level of cyclin E2 and clinical data was analyzed using the SPSS software. (sdu.edu.cn)