Cyclin e overexpression cyclin b. Medical search. Frequent questions

Additionally, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4) and retinoblastoma protein (pRb), was further down-regulated under exposure to lovastatin in condition of BRCA1 overexpression, but the expression of p21WAF1/CIP1, a cyclin-dependent kinase inhibitor (CDKI), was further up-regulated, both in vitro and in vivo detected with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis. (nih.gov)
It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. (origene.com)
The overexpression of miRNA‑375 significantly increased caspase‑3 and caspase‑9 activities, induced B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein, tumor protein 53 and cyclin‑dependent kinase inhibitor 1 protein expression and suppressed cyclin D1 and survivin protein expression in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
In addition, numerous important cellular proteins, such as B-cell lymphoma-2 homologous antagonist/killer, Myc proto-oncogene protein, cyclin-dependent kinase inhibitor 1B, DNA replication licensing factor MCM-7, retinoblastoma 1 and Annexin A1, are degenerated through the UBE3A-mediated ubiquitin proteasome pathway ( 7 ). (spandidos-publications.com)

The purpose of this study was to investigate whether breast cancer susceptibility gene 1 (BRCA1) overexpression sensitizes mammary cancer cells to statins. (nih.gov)
G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. (wikipedia.org)
There was no significant difference of overexpression of cyclin D1 or c-Myc gene products between these four histologic subtypes of ovarian adenocarcinomas. (bmj.com)
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (novusbio.com)
The molecular consequence of translocation is overexpression of the protein cyclin D1 (coded by the PRAD1 gene located close to the breakpoint). (medscape.com)
Background/Aim: Cyclin D1 gene (CCND1) has a G to A polymorphism at the splice donor site of exon 4, position 870. (iiarjournals.org)
Cyclin D1, a 45-kDa protein encoded by the cyclin D1 gene ( CCND1 ) on 11q13, is one such molecule. (iiarjournals.org)
Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (genetex.com)
The translocation probe is designed to detect the juxtaposition of the IGH locus (14q32) next to the CCND1 (cyclin D1) gene (11q13) in both metaphase and interphase nuclei. (mplnet.com)
Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma. (bvsalud.org)

Previous work has shown that high cyclin B1 expression levels are found in variety of cancers such as breast, cervical, gastric, colorectal, head and neck squamous cell, non-small-cell lung cancer, colon, prostate, oral and esophageal. (wikipedia.org)
Solid as well as blood cancers are associated with overexpression of HOX proteins in the nucleus, which is traditionally a difficult area of the cell to target with conventional drugs. (nottingham.ac.uk)
This family of RTKs has been highly implicated in the formation and progression of various cancers via aberrant overexpression, kinase activation, and mutation [1] , [2] . (plos.org)
Overexpression or genetic activation of Cyclin Dependent Kinase 2 (CDK2) binding partner cyclin E is a key oncogenic process in several cancers. (worldpharmanews.com)
IL-33 overexpression in gallbladder cancers associated with pancreatobiliary maljunction. (cdc.gov)

S-phase kinase-associated protein 2 (SKP2) is an important cell cycle regulator, targeting the cyclin-dependent kinase (CDK) inhibitor p27 for degradation, and is frequently overexpressed in breast cancer. (umass.edu)
Dysregulation of CDK8 (Cyclin-Dependent Kinase 8) and its regulatory partner CycC (Cyclin C) , two subunits of the conserved Mediator (MED) complex, have been linked to diverse human diseases such as cancer. (sdbonline.org)
Orthologous to human CDK2AP1 (cyclin dependent kinase 2 associated protein 1). (nih.gov)
Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. (nih.gov)
Cell-cycle arrest was associated with the engagement of checkpoint kinase 2-cell division cycle 25C-cyclin-dependent kinase 1/cyclin B1 signaling. (bioone.org)
They are able to build complexes with cyclin-dependent kinases (CDKs) and regulate their activity ( 2 ). (iiarjournals.org)
Similarly, depletion in the STRIPAK component STRIP1 affects activation of GCKIII kinases and cell cycle disruption through elevated expression of cyclin dependent kinase inhibitors p21 and p27, enhanced levels of which lead to a protective effect from therapeutic treatments and increased proliferation. (lu.se)

The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. (cancerindex.org)

Twenty-eight (30.4%) of the carcinoma specimens showed overexpression of cyclin D1. (elsevierpure.com)
This study was designed to determine the relationship between cyclin D1 overexpression and clinicopathological prognostic factors in breast invasive ductal carcinoma Methods- In this descriptive-analytical cross-sectional investigation, 89 patients with invasive ductal breast cancer attending to Alzahra Hospital, Isfahan, Iran from 2003 to 2008 entered the study. (ac.ir)
Cyclin D1 overexpression has been associated with parathyroid carcinoma. (medscape.com)
Association of cyclin D1, p16 and retinoblastoma protein expressions with prognosis and metastasis of gallbladder carcinoma. (cdc.gov)

Higher scores of cyclin DI expression were more frequent in lower tumor grade and tumors with positive ER and PR.Conclusion- According to the obtained results, it seems that overexpression of cyclin Dl in breast cancer is associated with some favorable prognostic factors including lower grade and ER and PR positive status. (ac.ir)
To investigate the expression pattern of G1 cyclins in 1,2- dimethyl-hydrazine dihydrochloride (DMH)-induced rat colon carcinogenesis, we studied the expression of cyclin D1 and cyclin E by quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry (IHC). (ewha.ac.kr)
Cyclin D1 expression was detected in 56.3% of the adenomas and in 61.5% of the adenocarcinomas examined, whereas cyclin E expression was detected in 87.5% of the adenomas and in 92.3% of the adenocarcinomas. (ewha.ac.kr)
Overall, cyclin D1, cyclin E and PCNA expression was significantly increased at both the mRNA and protein levels in normal colonic mucosa, adenomas and adenocarcinomas, but there was no significant difference in the degree of expression of these genes in adenomas and adenocarcinomas. (ewha.ac.kr)
Wild-type p53 have been shown to suppress cyclin B1 expression. (wikipedia.org)
For example, an increase in expression of cyclin B1/cdc2 is significantly higher in breast tumor tissue and shown to increase lymph node metastasis in breast cancer. (wikipedia.org)
Nuclear-dominant expression of cyclin B1 leads to poorer prognosis due to its weak activity compared to cytoplasmic cyclin B1. (wikipedia.org)
No abnormal expression of cyclin D1 or c-Myc was demonstrated in any of the 12 normal ovarian specimens. (bmj.com)
Although our preliminary results need to be validated in a larger number of tumors, the abnormal expression of cyclin D1 and c-Myc in epithelial ovarian cancer reaffirms the notion that they are crucial components in the pathway of tumorigenesis and deserve further study. (bmj.com)
Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - CCNH monoclonal antibody (M01), clone 1B8 Analysis of CCNH expression in HeLa. (novusbio.com)
Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (novusbio.com)
Further, the overexpression of HER3 enhanced cyclin D1 mRNA expression, while HER3 deleted of its identified TADs was hindered at doing so. (plos.org)
The overexpression of miRNA‑375 significantly suppressed the levels of protein expression of ubiquitin‑protein ligase E3A (UBE3A) and Insulin‑like growth factor‑1 receptor (IGF‑1R) in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
Expression of cyclin D1 has been reported to be a strong predictor for the clinical outcome of OSCC ( 4 ), and amplification of CCND1 was found significantly more frequently in patients with OSCC than in healthy controls ( 5 ), suggesting the predictive and prognostic value of this molecule and its potential as a therapeutic target. (iiarjournals.org)
Activation of PKCd with a phorbol ester leads to elevated cyclin D1 expression and an hyperphosphorylated Rb state. (conicet.gov.ar)
Ki-67 expression both in the crypt-surface epithelium and cyclin D1, c-erb B2, bcl-2 over-expression may be a clue of dysplastic epithelium. (uwi.edu)
This altered location of the IGH locus is thought to cause over expression of cyclin D1 in mantle cell lymphomas. (mplnet.com)
This assay is an excellent alternative to immunoperoxidase detection of cyclin D1 over expression. (mplnet.com)
NFAT2 overexpression induced the expression of Egr2 and FasL and suppressed the phosphorylation of AKT and ERK. (researchsquare.com)
Epstein-Barr virus latent membrane protein expression and cyclin DI cell cyase protein expression in malignant and normal oesophageal tissues to see whether any variation in their expression in these tissues could be of diagnostic or prognostic value. (bvsalud.org)
EBV-LMPI protein expression and cyclin DI expression were studied immunohisto chemically in these tissue sections. (bvsalud.org)
The mechanism by which MSLN contributes to these more aggressive behaviors was investigated by using ingenuity pathway analysis, which predicted that increased MSLN could induce cyclin E expression. (cdc.gov)

Cyclins are a group of proteins that play a key role in the control and regulation of the cell cycle. (iiarjournals.org)

Abstract: Background- Cyclin DI level begins to rise early in GI phase of cell cycle and continues to accumulate until the G 1/S-phase boundary, when the level rapidly declines. (ac.ir)

Cyclins function as regulators of CDK kinases. (novusbio.com)

This overexpression was not related to the tumor stage, lymph node metastasis, or clinical outcome. (elsevierpure.com)

WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)

Firstly, the overexpression of miRNA‑375 significantly suppressed cell proliferation, increased lactate dehydrogenase activity and induced apoptosis in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
Overexpression of IGF-1 in serum and tissue alters the growth of normal cells and causes uncontrolled proliferation, inhibits differentiation and reduces apoptosis, resulting in the incidence and development of malignant tumors ( 10 ). (spandidos-publications.com)
The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)

We immunohistochemically investigated the overexpression of cyclin D1 protein in 92 laryngeal carcinomas. (elsevierpure.com)
This study shows that cyclin D1 and c-Myc are frequently overexpressed in epithelial ovarian carcinomas, but they are not correlated with a particular histologic subtype. (bmj.com)

Our results suggest that BRCA1 overexpression sensitizes cancer cells to lovastatin via regulation of cyclin D1-CDK4-p21WAF1/CIP1 pathway, which will provide an innovative experimental framework to study control of breast cancer cell proliferation. (nih.gov)
Overexpression of PKCd in NMuMG cells leads to a significant increase in [3H]-tymidine incorporation and cell proliferation, as well as enhanced ERK-MAPK activation. (conicet.gov.ar)
We found that BSW shMSLN cells had decreased cyclin E, and their proliferation rate was reverted to nearly that of untransformed cells. (cdc.gov)

Cytogenic analysis and comparative genomic hybridization (CGH) studies have demonstrated that both WDLPS and DDLPS are characterized by supernumerary ring chromosomes or giant marker chromosomes and12q13-15 region amplifications, resulting in MDM2 and CDK4 overexpression. (sarcomahelp.org)
This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)

MCF-7 cells, which have only one wild-type BRCA1 allele, were transfected with pcDNA3-beta-HA-hsBRCA1 plasmids via liposomes to reconstitute BRCA1 overexpression human breast cancer cell line, and tumoral xenografts with BRCA1 overexpression were subsequently established in BALB/c nude mice. (nih.gov)
Our results indicate that the overexpression of cyclin D1 and cyclin E may play an important role during the multistage process of rat colon carcinogenesis, at a relatively early stage, and may disturb cell-cycle control in benign adenomas, and thereafter, participate in tumor progression. (ewha.ac.kr)
Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. (wikipedia.org)
Like all cyclins, levels of cyclin B1 oscillate over the course of the cell cycle. (wikipedia.org)
Just prior to mitosis, a large amount of cyclin B1 is present in the cell, but it is inactive due to phosphorylation of Cdk1 by the Wee1 kinase. (wikipedia.org)
A possible trigger for activation is phosphorylation by cyclin A-Cdk, which functions before cyclin B1-Cdk in the cell cycle. (wikipedia.org)
Before mitosis almost all cyclin B1 in the cell is located in the cytoplasm, but in late prophase it relocates to the nucleus. (wikipedia.org)
At the end of mitosis, cyclin B1 is targeted for degradation by the APC through its APC localization sequence, permitting the cell to exit mitosis. (wikipedia.org)
The role of cyclin B1 is to transition the cell from G2 to M phase but becomes unregulated in cancer cells where overexpression of cyclin B1 can lead to uncontrolled cell growth by binding to its partner Cdks. (wikipedia.org)
Cyclin D1 and c-Myc are key participants in the cell-cycle pathway, in which aberrancies have been associated with malignant transformation. (bmj.com)
Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. (novusbio.com)
This truncated variant, cyclin D1b, does not have the part which is required for export from the nucleus into other cell localizations and therefore has higher transforming activity ( 8 ). (iiarjournals.org)
Specifically speaking, after transfecting liver tumor cell lines with antisense nucleic acids or mimics of miR-122, the inhibition of HBV replication was found by overexpression of miR-122 and promotion of HBV replication by depletion of endogenous miR-122 [ 14 ]. (hindawi.com)
MSLN overexpression was found in human lung tumors, lung cancer cell lines, and BSW cells. (cdc.gov)
Targeted overexpression or knockdown of PIP5K1α were introduced into BC cell lines. (lu.se)

Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. (novusbio.com)
Sandwich ELISA: Cyclin H Antibody (1B8) [H00000902-M01] - Detection limit for recombinant GST tagged CCNH is approximately 1ng/ml as a capture antibody. (novusbio.com)
IHC-P analysis of human tonsil tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
There are currently no references for Cyclin D1 antibody [RM241] (GTX33611) . (genetex.com)

[ 7 ] Parafibromin can negatively regulate cyclin D1 and may be responsible at least in part for this. (medscape.com)
In ER+ cancer cells, PIP5K1α acted on pSer-473 AKT, and was in complexes with VEGFR2, serving as co-factor of ER-alpha to regulate activities of target genes including cyclin D1 and CDK1. (lu.se)

Immunohistochemical staining revealed exclusive nuclear staining of the neoplastic cells for cyclin D1, cyclin E and PCNA. (ewha.ac.kr)
Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. (wikipedia.org)
Another important function of the cyclin B1-Cdk1 complex is to break down the nuclear envelope. (wikipedia.org)
Phosphorylation of the lamins by cyclin B1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. (wikipedia.org)
Phosphorylation of cyclin B1 causes it to be imported to the nucleus, and phosphorylation also prevents export from the nucleus by blocking the nuclear export signal. (wikipedia.org)
Next, we identified full-length nuclear HER3 association and regulation of a 122 bp region of the cyclin D1 promoter. (plos.org)
To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)

Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. (wikipedia.org)
Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. (wikipedia.org)
Once cyclin B1-Cdk1 is activated, it remains stably active for the rest of mitosis. (wikipedia.org)
Another mechanism by which cyclin B1-Cdk1 activity is regulated is through subcellular localization. (wikipedia.org)
This is regulated by the phosphorylation of cyclin B1, in contrast to phosphorylation of Cdk1 regulating the activity of the complex. (wikipedia.org)
Cyclin B1 is phosphorylated by Polo kinase and Cdk1, again setting up a positive feedback loop that commits cyclin B1-Cdk1 to its fate. (wikipedia.org)
Cyclin B1 has been shown to interact with Cdk1, GADD45A and RALBP1. (wikipedia.org)

SKP2 overexpression is associated with increased serine 10 phosphorylation of p27 (pSer10p27) in triple-negative breast cancer. (umass.edu)

Deregulation of G1 cyclins has been reported in several human and rodent tumors including colon cancer. (ewha.ac.kr)
However, compared to normal ovarian tissues, overexpression of cyclin D1 and c-Myc was observed in 42.6% (20/47) and 65.9% (31/47) of tumors examined, respectively. (bmj.com)

Cyclin B1 can reside in the nucleus or the cytoplasm which can have an effect on the malignant potential of cyclin B1 when overexpressed in each location. (wikipedia.org)

Overexpression of cyclin D1 has been found in a variety of malignancies and is suggested to be related to tumor progression. (elsevierpure.com)
Moreover, overexpression of this cyclin was found to be a prognostic factor determining resistance to fulvestrant and shorter progression-free survival. (lu.se)

This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. (novusbio.com)
p27 regulates G1 /S transition by abrogating the activity of cyclin/CDK complexes. (umass.edu)

While the exact mechanism that explains how cyclin B1 becomes overexpressed is not very well understood, previous work has shown that down regulation of cyclin B1 can lead to tumor regression. (wikipedia.org)

Overexpression of JAM3 or recombinant JAM3 protein enhanced trophoblast adhesion and migration, which were inhibited by JAM3 knockdown. (bioone.org)

Cyclin B1 is a regulatory protein involved in mitosis. (wikipedia.org)

Cyclin D1 staining was performed on paraffin- embedded tumor tissue samples and the relationship between cyclin DI status and age, tumor grade, tumor stage, estrogen receptor (ER), progesterone receptor (PR), and HER2- Neu status was evaluated. (ac.ir)

These high levels of cyclin B1 can also be associated to the extent of tumor invasion and aggressiveness therefore concentration of cyclin B1 can be used to determine the prognosis of cancer patients. (wikipedia.org)

To identify upstream regulators or downstream effectors of CDK8, a dominant modifier genetic screen was performed in Drosophila based on the defects in vein patterning caused by specific depletion or overexpression of CDK8 or CycC in developing wing imaginal discs. (sdbonline.org)

The A allele codes for a truncated variant, cyclin D1b, which may have higher transforming activity. (iiarjournals.org)

Cyclin Di is one of the most commonly overexpressed oncogenes in breast cancer. (ac.ir)

La concentration sérique pour ces facteurs chez les femmes non encein- tes était similaire à celle des femmes enceintes en bonne santé. (who.int)
Toutefois, la concentration sérique était significativement plus élevée en présence d'une prééclampsie bénigne ou grave par rapport aux grossesses normales et significativement plus élevée pour les prééclampsies graves par rapport aux prééclampsies bénignes. (who.int)

The sensitivity and Ca 2+ mobilization of HepG2 cells was also inhibited by NFAT2 overexpression. (researchsquare.com)

Moreover, we found the further reduced volume of tumoral xenografts treated with lovastatin in the presence of BRCA1 overexpression. (nih.gov)

pointed out that miR-122 affects both p53 and cyclin G-1 and consequently HBV replication [ 13 ]. (hindawi.com)

However, the overexpression of cyclin D1 in patients with local recurrence was significantly higher than in patients with no recurrence. (elsevierpure.com)

This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. (novusbio.com)

Diseases1

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