Loading...
  • genes
  • This has launched the development of novel anticancer agents, interfering with the proteins encoded by the identified mutated genes. (embl.de)
  • The Ras protein is involved in signal transduction: it passes on stimuli from extracellular factors to the cell nucleus, thereby changing the expression of a number of growth regulating genes. (embl.de)
  • The invention provides oligonucleotide probes and primers, polynucleotide plasmids or vectors, peptides, proteins, and antibodies relating to genes and gene products associated with the senescence in mammalian cells. (google.es)
  • The inactivated virus caused the up-regulation of a much greater number of mRNAs, many of which encoded proteins with antiviral roles, such as interferon-responsive genes and proinflammatory cytokines. (broadinstitute.org)
  • Phenotypic anchoring of the expression data to DNA adduct levels detected by 32 P-postlabelling, cell cycle data and p53 protein expression identified a number of genes that are linked to these biological outcomes, thereby strengthening the identification of target genes. (beds.ac.uk)
  • residues
  • These modified Ras proteins carry a number of different, natural and non-natural lipid residues, and the process was extended to also provide access to a number of fluorescently labeled derivatives. (embl.de)
  • Furthermore, a preliminary study on the biological activity of the natural Ras protein derivative (containing the normal farnesyl and palmitoyl lipid residues) has shown full biological activity. (embl.de)
  • Tumor suppression depended on residues in the C-terminal domain of plexin B1 which mediate receptor GAP activity, and also correlated with AKT inhibition. (pubmedcentralcanada.ca)
  • guanine
  • Of these, the most studied are the RAF kinase and phosphoinositide 3-kinase (PI3K) protein families and Ral guanine dissociation stimulator ( 5 - 8 ). (aacrjournals.org)
  • In response to ligand binding, Rho is activated by recruitment of guanine nucleotide exchange factors (GEFs) to receptors, and Rac is inhibited by direct receptor binding and sequestration ( 14 - 18 ). (pubmedcentralcanada.ca)
  • Son of sevenless ("Sos"), the Ras-specific guanine nucleotide exchange factor, couples RTK stimulation to Ras activation by undergoing ligand-stimulated recruitment to the plasma membrane where it promotes the conversion of Ras-GDP to Ras-GTP (Schlessinger et al. (patentsencyclopedia.com)
  • actin
  • We label cells with a ubiquitously expressed transgene that encodes GFP fused to an autonomously folding actin binding fragment from fly moesin. (rupress.org)
  • cellular
  • Apart from Ras, they also target other cellular proteins that require farnesylation to become activated, e.g. (embl.de)
  • Background: The solute carrier family 39 (zinc transporter) member 7 (SLC39A7, ZIP7) is an ER and Golgi membrane protein that regulates cellular zinc homeostasis by controlling release of zinc from these organelles to the cytoplasm (1,2). (cellsignal.com)
  • The cellular response to genotoxic stress may depend on the cell type being insulted, compound concentration and duration of exposure and it is important to understand the common and specific pathways of such responses. (beds.ac.uk)
  • fluorescent
  • Furthermore, access to bioconjugates that embody the correct structure of the protein but that may additionally carry different lipid groups or labels (i.e., fluorescent tags) by which the protein can be traced in biological systems, could provide invaluable reagents. (embl.de)
  • mRNA
  • By analyzing the mRNA expression profile of cells infected in the presence of cycloheximide, it was found that a minimum of 25 mRNAs were modulated by HCMV in the absence of protein synthesis. (broadinstitute.org)
  • The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium. (sdbonline.org)
  • pathway
  • Mutated Ras proteins remain longer in their active form than normal Ras proteins, resulting in an overstimulation of the proliferative pathway. (embl.de)
  • phosphatase
  • This is done through sending signals to phosphatase regulatory and catalytic subunits, plus specific inhibitor proteins. (virginia.edu)
  • Shah M, Smolko C, Kinicki S, Chapman Z, Brautigan D, Janes K. Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. (virginia.edu)
  • Ziembik M, Bender T, Larner J, Brautigan D. Functions of protein phosphatase-6 in NF-κB signaling and in lymphocytes. (virginia.edu)
  • antibody
  • This Cell Signaling Technology (CST) antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. (cellsignal.com)
  • synthesis
  • We report here of the development of techniques for the synthesis of a series of modified Ras proteins. (embl.de)
  • molecular
  • Current research in our group investigates the molecular basis for recognition among these proteins, and tests how phosphatases operate in living cells in response to changing conditions. (virginia.edu)
  • complex
  • A well established mechanism for the membrane recruitment of Sos involves the formation of a ternary complex with the activated RTK and the adaptor molecule Grb2, through the binding of the Grb2 SH3 domain to a proline-rich sequence in the C-terminus of Sos. (patentsencyclopedia.com)
  • family
  • The Ras family of small GTPases includes three closely related proteins: H-, K-, and N-Ras. (embl.de)
  • specific
  • The maleimide group provided the key to link chemically synthesized lipopeptide molecules in a specific and efficient manner to a truncated form of the H-Ras protein. (embl.de)
  • Human
  • The unconjugated Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 reacts with human, mouse, rat, hamster, Drosophila melanogaster, bovine and zebrafish phospho-Akt protein. (cellsignal.com)
  • results
  • In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. (cancerindex.org)