ApoptosisInhibitorsInducesProgressionComplexesProteinMitoticDifferentiationCDK4GenesProteinsCDK1Signaling pathwayInhibitorIrreversible cell cycle arrestInhibitionMetaphaseNeuroblastoma cell linesPathwaysSenescent cellsVitroPhosphorylationUpstreamHead and neck squamous cell carcCDK2ActivatesEpithelialUpregulationRegulatorEndothelialExpressionEukaryoticTranslationalTumor cellsDegradationActivityCancerIndicates that senescentTranscriptionRegulationMitogenicDisruptionHumanMechanismsRenal cell carcMitosisPromotesStimulatesYeastSuppressionMalignantOncogenicPhenotypeGrowth
Apoptosis17
- induces growth arrest or apoptosis depending on the physiological circumstances and cell type. (lsbio.com)
- LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. (lsbio.com)
- The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. (cubebiosystems.com)
- To investigate the putative anti-proliferative effect, apoptosis assay, microRNA expression profile and study of cell cycle were performed. (biomedcentral.com)
- Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
- Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
- Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
- In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
- These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
- These potent effects are driven by curcumin's ability to induce G2/M cell cycle arrest, induce autophagy, activate apoptosis, disrupt molecular signaling, inhibit invasion and metastasis, and increase the efficacy of current chemotherapeutics. (hindawi.com)
- Unlike apoptosis, senescence is a state of which cells are still alive and metabolically active. (frontiersin.org)
- The role of chemotherapy-driven increases in PTN/PTPRZ1 expression was examined with a CCK-8 assay, colony formation efficiency assay and apoptosis analysis with TNBC cells. (biomedcentral.com)
- p53 is recognized as a critical regulator of the cell cycle and apoptosis. (biomedcentral.com)
- The tumor suppressor p53 is recognized as a critical regulator of cell cycle progression and apoptosis, incorporating signals from DNA damage and other cellular stressors to decide cell fate [ 1 ]. (biomedcentral.com)
- The mitochondrial membrane potential (MMP), reactive oxygen species (ROS), cell cycle, apoptosis, DNA damage, and caspase 3 activity were analyzed by flow cytometry, and the expression profiles of different anti- and pro-apoptotic as well as epigenetic signals were studied by immunoblotting. (molcells.org)
- NQ induced apoptosis in HepG2 cells by activating p53-ROS crosstalk and induces epigenetic modifications leading to inhibited proliferation and cell cycle arrest. (molcells.org)
- 2006. Accelerated degradation of FADD and procaspase 8 in cells expressing human papilloma virus 16 E6 impairs TRAIL-mediated apoptosis. (virosin.org)
Inhibitors11
- Targeted drugs such as thyrosine kinase inhibitors and cyclin-dependent kinase inhibitors are widely used in cancer patients. (researchsquare.com)
- Ribociclib and palbociclib are oral cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors that arrest the cell cycle by inhibiting DNA synthesis inhibition[9]. (researchsquare.com)
- This senescence-like development arrest induced by is certainly associated with deposition of development inhibitors such as for example p53 and p16INK4A (51). (molecularcircuit.com)
- They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
- We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
- Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
- Growth factors do not only stimulate cell proliferation, but they may also act as growth inhibitors, depending on the cell type and the stimulatory pathway that is involved. (biomedcentral.com)
- It has also been demonstrated that TGF-β induced cell cycle arrest can be partially attributed to the regulatory effects of TGF-β on both the expression and activity of cyclin-dependent kinase inhibitors [CDKI] such as p21 and p27. (biomedcentral.com)
- Current scientific trials are looking into cyclin-dependent kinase (CDK) inhibitors, such as for example roscovitine, an inhibitor of CDK1, -2, -5, -7, and -9, that lowers viral DNA synthesis, creation of late protein and infectious pathogen particles [4]. (niepokorny.org)
- Furthermore, we previously confirmed the fact that selective CDK9 and CDK7 inhibitors R22 and LDC4297 exert solid anticytomegaloviral activity in cell lifestyle versions [9]. (niepokorny.org)
- The defining characteristic of senescence is a highly stable cell cycle arrest, triggered by the up-regulation of cyclin-dependent kinase inhibitors such as p16 INK4a and p21 CIP1a . (rupress.org)
Induces5
- In primary mammalian cells oncogenic induces premature senescence depending on an active MEK-extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) pathway. (molecularcircuit.com)
- Ectopic expression of MEKK3 a MAPKKK that activates p38 induced G1 arrest and reversed induces premature senescence through sequential activation of the MEK-ERK pathway and the MKK3/6-p38 pathway in primary human fibroblasts. (molecularcircuit.com)
- Functionally, miR-193b induces a G1 cell cycle arrest and cell death in neuroblastoma cell lines by reducing the expression of MYCN , Cyclin D1 and MCL-1 , three important oncogenes in neuroblastoma of which inhibition has shown promising results in preclinical testing. (oncotarget.com)
- Genistein induces G2/M phase arrest by down-regulating Cyclin B1 expression through suppression of Akt phosphorylation and GPR30 activation in BRCA1 deficient breast cancer cells. (snu.ac.kr)
- 2011. Human papillomavirus type 16 E6 induces cervical cancer cell migration through the p53/microRNA-23b/urokinase-type plasminogen activator pathway. (virosin.org)
Progression10
- Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. (lsbio.com)
- This autonomous Cdc14 release oscillator functions at constant cyclin-Cdk levels by titrated introduction of undegradable Clb2, and at cell-cycle-average Clb2 levels given a block of cell cycle progression by actin depolymerization. (rockefeller.edu)
- It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- � � induced G1 arrest. (neobiotechnologies.com)
- Important regulator of cell cycle progression. (neobiotechnologies.com)
- It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
- Effects of genistein on cell cycle progression were examined by flow cytometry. (snu.ac.kr)
- In addition with their main function in the legislation of cell routine progression, particular types of CDKs and cyclins get excited about transcription also, splicing, epigenetic legislation, neuronal features, stem cell regeneration, spermatogenesis, and differentiation [10]. (niepokorny.org)
- Oocyte maturation involves the release of the primary meiotic arrest at prophase I and progression of the oocyte nucleus into meiotic divisions to produce a mature oocyte (egg) capable of being fertilized ( Von Stetina and Orr-Weaver, 2011 ). (elifesciences.org)
- Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. (arigobio.cn)
- Genes necessary for cell cycle progression, such as E2F-dependent genes, are incorporated into the SAHF and are thereby silenced, contributing to the stability of the growth arrest. (rupress.org)
Complexes6
- This implies that even after induction of stable nuclear cyclin D3-CDK4 complexes, dog thyrocytes still depend on cAMP for RB phosphorylation and commitment to DNA synthesis, which suggests that a key labile event responsible for a last control of restriction point passage remains to be uncovered, in the cAMP-dependent cell cycle of dog thyrocytes and possibly other systems. (ac.be)
- Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. (neobiotechnologies.com)
- Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. (neobiotechnologies.com)
- Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. (neobiotechnologies.com)
- In HCMV-infected cells, particular subsets of CDK-cyclin complexes are downregulated/suppressed (CDK4-cyclin D, CDK6-cyclin D, CDK2-cyclin A) or upregulated/turned on (CDK1-cyclin B, CDK2-cyclin E), leading to an early on S stage arrest termed pseudomitosis [11] eventually. (niepokorny.org)
- During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. (arigobio.cn)
Protein16
- Immunohistochemistry in diagnostic surgical pathology: contributions of protein life-cycle, use of evidence-based methods and data normalization on interpretation of immunohistochemical stains. (lsbio.com)
- Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. (affbiotech.cn)
- Hartwell shared the 2001 Nobel Prize in Physiology and Medicine for discoveries of protein molecules that control the division (duplication) of cells. (historyofmedicine.com)
- As far as LEV is concerned, in 2010 Bobustuc hypothesized that AED may modulate O-6 methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that has an important role in tumor cell resistance to alkylating agents, and LEV was reported as the most potent MGMT inhibitor among several AED [ 10 ]. (biomedcentral.com)
- This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. (neobiotechnologies.com)
- Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
- The number of encoded NBPF/DUF1220 repeats varies from 4 to 52 copies, depending on the gene member and the NBPF1 protein has 7 repeats [ 6 ]. (biomedcentral.com)
- The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
- Furthermore to people indirect results on cell routine legislation, the viral proteins kinase pUL97 straight cross-talks with CDKs since it mimics CDKs in phosphorylating partly-identical substrates and evidently possesses commonalities in protein framework and functionality. (niepokorny.org)
- Recently, multiple lines of evidence suggested that the expression of pleiotrophin (PTN), which is a secreted growth factor that binds to receptor protein tyrosine phosphatase zeta (PTPRZ1) to stimulate human endothelial cell migration, is associated with poor prognosis in a variety of malignant tumours [ 6 ]. (biomedcentral.com)
- The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. (sitoolsbiotech.com)
- The data show p53 protein was transcriptionally activated and contributed to NGF-mediated neurite outgrowth during differentiation of PC12 cells. (biomedcentral.com)
- Discovery of non-genotoxic mechanisms through which p53 protein may be activated within tumor cells harboring wild-type p53 remains an important therapeutic objective, both in effort to alleviate traditional off-target chemotherapeutic side-effects and to prevent the unpredictable mutagenicity characteristic of these therapies. (biomedcentral.com)
- Analysis of G(1) regulatory proteins demonstrated that protein levels of CDK2, CDK4, cyclin D1, and cyclin A were decreased in a time-dependent manner, but not those of CDK6 and cyclin E, by EB1089. (drlauda.at)
- Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. (arigobio.cn)
- The foamy stromal cells between the capillaries stain positive for glial fibrillary acid protein and neuron-specific enolase. (medscape.com)
Mitotic6
- Cdc14 release and ME is controlled by mitotic cyclin-Cdk oscillation, the FEAR network including a non-proteolytic function of separase (Esp1), and the Mitotic Exit Network (MEN) indirectly activated by spindle elongation through cohesin cleavage by the proteolytic function of Esp1. (rockefeller.edu)
- Reducing mitotic cyclin-Cdk activity is critical for ME, but Cdc14 release and resequestration is not blocked by endogenous undegradable mitotic cyclin Clb2. (rockefeller.edu)
- Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
- However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)
- Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. (arigobio.cn)
- The human papillomavirus type 16 E6 and E7 oncoproteins cooperate to induce mitotic defects and genomic instability by uncoupling centrosome duplication from the cell division cycle. (virosin.org)
Differentiation5
- It inhibits cytokine production and cell proliferation/differentiation in various immune cells. (uwo.ca)
- It has been reported that TGF-β influence different cell functions, including growth, proliferation and differentiation. (biomedcentral.com)
- Mounting evidence also suggests a role for p53 in differentiation of cells including neuronal precursors. (biomedcentral.com)
- We studied the transcriptional role of p53 during nerve growth factor-induced differentiation of the PC12 line into neuron-like cells. (biomedcentral.com)
- p53 transcriptional activity may be further involved in the differentiation of other non-neuronal cell types. (biomedcentral.com)
CDK41
Genes8
- Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. (lsbio.com)
- One of the activated genes is an inhibitor of cyclin-dependent kinases. (lsbio.com)
- Genetics had divided the cycle into two parallel pathways, which comprised two sets of dependent steps and involved a total of 19 cdc genes. (historyofmedicine.com)
- We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
- Gene Ontology (GO) enrichment analysis of these genes identified an overrepresentation of genes related to the cell cycle. (biomedcentral.com)
- We discovered an unprecedented burst of differential expression of cell cycle related genes at one particular point during transition to flowering. (biomedcentral.com)
- Although senescent cells repress proliferation-promoting genes, they also induce the gene program necessary for the implementation of senescence. (rupress.org)
- Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
Proteins8
- The proto-oncogene family members encodes little GTP binding proteins that transduce development indicators from cell surface area receptors in response to extracellular stimuli (1 6 37 Prior studies have recommended that aberrant activation of is certainly a crucial stage during tumorigenesis. (molecularcircuit.com)
- In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
- We demonstrated that NBPF1 exerts different tumor suppressive effects, depending on the cell line analyzed, and provide new clues into the molecular mechanism of the enigmatic NBPF proteins. (biomedcentral.com)
- But it is not associated with cell cycle proteins. (biomedcentral.com)
- CDKs are cyclin-dependent serine-/threonine-specific proteins kinases, the experience of which depends upon cyclin binding. (niepokorny.org)
- We determined by circular dichroism spectroscopy that NQ effectively caused conformational changes in DNA and modulated different proteins related to epigenetic modifications and c ell cycle control. (molcells.org)
- In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. (arigobio.cn)
- The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
CDK13
- Along with CDK1 and -2, CDK7 and -9 are necessary for effective HCMV replication and had been discovered upregulated in HCMV-infected cells [3,12,13,14,15]. (niepokorny.org)
- In all five HPV negative and HPV positive cell lines tested, roscovitine caused inhibition of CDK1 and 2. (oncotarget.com)
- CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. (arigobio.cn)
Signaling pathway3
- However, THP-1 cells adaptively adjusted to LeTx and overrode cell cycle arrest by activating the PI3K/Akt signaling pathway. (uwo.ca)
- Planar Cell Polarity (PCP), the polarization within the plane of an epithelium, is perpendicular to apical-basal polarity and established by the non-canonical Wnt/Fz-PCP signaling pathway. (uni-wuerzburg.de)
- In this study, I investigated the proliferation signaling pathway in triple negative breast cancer (TNBC) cells with respect to the presence of functional BRCA1 and whether this signaling can be inhibited by genistein. (snu.ac.kr)
Inhibitor6
- The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
- Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
- Here we tested whether roscovitine, an inhibitor of cyclin-dependent kinases (CDKs), which hereby also blocks homologous recombination (HR), can be used to enhance the radiation sensitivity of HNSCC cell lines. (oncotarget.com)
- Due to a defect in HR, all HPV positive cell lines were efficiently radiosensitized by the PARP-1 inhibitor olaparib. (oncotarget.com)
- PMID: 10102234 [PubMed - indexed for MEDLINE] 1: Exp Cell Res 2000 Feb 1;254(2):279-86 Cell cycle arrest induced by the vitamin D(3) analog EB1089 in NCI-H929 myeloma cells is associated with induction of the cyclin-dependent kinase inhibitor p27. (drlauda.at)
- The prolyl oligopeptidase inhibitor SUAM-14746 attenuates the proliferation of human breast cancer cell lines in vitro. (cancerindex.org)
Irreversible cell cycle arrest2
- Aging kidney and CKD share many common characteristic features with increased cellular senescence, a conserved program characterized by an irreversible cell cycle arrest with altered transcriptome and secretome. (frontiersin.org)
- Cellular senescence was first identified as a type of irreversible cell cycle arrest that occurs when cells reach the end of their replicative potential ( Hayflick and Moorhead, 1961 ). (rupress.org)
Inhibition4
- Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells. (lsbio.com)
- In human monocytic cell lines U-937 and THP-1, LeTx induced cell cycle arrest in Go-Gi phase by rapid down-regulation of cyclin D1/D2 and checkpoint kinase 1 through MEK1 inhibition. (uwo.ca)
- particularly, the effect of miR-195-5p modulation seemed to affect cell cycle, while miR-107 seemed to be implicated in the inhibition of cells migration. (biomedcentral.com)
- Likewise, MDA-MB-231 cells that were transfected with BRCA1-siRNA were more susceptible to genistein-mediated growth inhibition. (snu.ac.kr)
Metaphase2
- Esp1 overexpression drives ME in Cdc20-depleted cells arrested in metaphase. (rockefeller.edu)
- The mature oocyte is arrested in metaphase II in most vertebrates and metaphase I in insects. (elifesciences.org)
Neuroblastoma cell lines4
- We show that miR-193b is expressed at low levels in neuroblastoma cell lines and primary tumor samples. (oncotarget.com)
- Introduction of miR-193b mimics into nine neuroblastoma cell lines with distinct genetic characteristics significantly reduces cell growth in vitro independent of risk factors such as p53 functionality or MYCN amplification. (oncotarget.com)
- Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)
- Additionally, forced expression of NBPF1 in two p53-mutant neuroblastoma cell lines also resulted in a G1 cell cycle arrest and CDKN1A upregulation. (biomedcentral.com)
Pathways6
- The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
- Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
- Therefore oncogenic provokes premature senescence by activating the MEK-ERK and MKK3/6-p38 pathways in normal primary cells sequentially. (molecularcircuit.com)
- At the beginning of the cell cycle, both pathways depend on the completion of a step that Hartwell et al. (historyofmedicine.com)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- The potential upstream pathways involved in the chemotherapy-driven increases in PTN/PTPRZ1 expression in TNBC cells were explored using microarray analysis, and the downstream mechanism was dissected with siRNA. (biomedcentral.com)
Senescent cells6
- Furthermore, senescent cells, with the secretory features known as the senescence-associated secretory phenotype (SASP), could produce proinflammatory cytokines, such as tumor necrosis factor- α (TNF- α ), interleukin-6 (IL-6), and monocyte chemoattractant protein1 (MCP-1), to greatly affect the neighboring cells [ 17 , 18 ]. (hindawi.com)
- Senescent cells elicit their fibrogenic actions primarily by secreting an assortment of inflammatory and profibrotic factors known as the senescence-associated secretory phenotype (SASP). (frontiersin.org)
- We also highlight potential options of targeting senescent cells for the treatment of CKD. (frontiersin.org)
- We also highlight potential options for targeting senescent cells in developing therapeutics for CKD patients. (frontiersin.org)
- However, the accumulation of senescent cells can have detrimental consequences, such as in age-related pathologies. (rupress.org)
- Senescent cells also undergo dramatic changes in their morphology and in the organization and architecture of their cellular compartments. (rupress.org)
Vitro7
- Aim of this study was to evaluate possible in vitro cytotoxic effects of two new-generation antiepileptic drugs on a human glioma cell line U87MG. (biomedcentral.com)
- Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
- Here, we developed CellAgeClock, a new epigenetic clock that measures subtle ageing changes in primary human cells in vitro . (biorxiv.org)
- Our method expands the scope of CpG methylation profiling from measuring human chronological and biological age from human samples in years, to accurately and rapidly detecting anti-ageing potential of drugs using human cells in vitro , providing a novel accelerated discovery platform to test sought after geroprotectors. (biorxiv.org)
- An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
- Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
- EB1089, a 1,25-dihydroxyvitamin D(3) analog, has been known to have potent antiproliferative properties in a variety of malignant cells in vitro and in vivo. (drlauda.at)
Phosphorylation3
- In primary cultures of dog thyroid epithelial cells, kinetic experiments indicate that a labile cAMP-dependent event positively controls a late G1 commitment to DNA replication and RB phosphorylation. (ac.be)
- Diminished phosphorylation of Akt in GPR30-siRNA transfected HCC1937 cells suggests regulation of Akt signaling by GPR30. (snu.ac.kr)
- Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. (arigobio.cn)
Upstream1
Head and neck squamous cell carc2
- At present, advanced stage human Papillomavirus (HPV) negative and positive head and neck squamous cell carcinoma (HNSCC) are treated by intense multimodal therapy that includes radiochemotherapy, which are associated with relevant side effects. (oncotarget.com)
- Two main etiologies of head and neck squamous cell carcinoma (HNSCC) are recognized that reveal distinct biological characteristics. (oncotarget.com)
CDK21
- Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). (neobiotechnologies.com)
Activates1
- Taken together, lack of functional BRCA1 activates GPR30 signaling, thereby phosphorylating Akt and promoting cell proliferation. (snu.ac.kr)
Epithelial1
- Transforming growth factor-β [TGF-β] is such an example, being a growth stimulator in fibroblastic cells with TGF-β receptors, but a negative regulator in epithelial cells. (biomedcentral.com)
Upregulation1
- However, CDKN1A upregulation by NBPF1 was not observed in the DLD1 cells, which demonstrates that NBPF1 exerts cell-specific effects. (biomedcentral.com)
Regulator1
- Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
Endothelial3
- Expression of chemoresistance molecules was evaluated using flow cytometry in U87MG and human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)
- Other reports also show that DN is highly associated with accelerated aging in various types of cells such as tubular cells, podocytes, mesangial cells, and endothelial cells [ 21 - 23 ]. (hindawi.com)
- [ 2 ] Diagnosed in 50% of patients with VHL disease, these hemangiomas are composed of endothelial cells and pericytes. (medscape.com)
Expression9
- Microinjection of a p38-encoding plasmid into NIH 3T3 fibroblasts led to down-regulation of cyclin D1 expression and cell cycle arrest at G1 (40). (molecularcircuit.com)
- To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented with wildtype or mutant B-Raf expression vectors. (uni-wuerzburg.de)
- Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix. (neobiotechnologies.com)
- Elevated expression of cell proliferation targets P-Akt and GPR30 was observed in BRCA1 mutated HCC1937 cells. (snu.ac.kr)
- LC-MS/MS analysis was used to investigate the proteome of DLD1 colon cancer cells with induced NBPF1 expression. (biomedcentral.com)
- We also aimed to evaluate the expression of TGF-β1 and p27 in the context of other cell cycle and proliferation markers such as cyclin D1 and Ki-67. (biomedcentral.com)
- CDKN1A was the critical switch that regulated the expression of PTN/PTPRZ1 in TNBC cells receiving chemotherapy. (biomedcentral.com)
- Our current studies showed that the expression of PTN and its receptor PTPRZ1 in human breast cancer tissue depended on whether the patient received chemotherapy, and chemotherapy-driven increases in PTN/PTPRZ1 signalling could inhibit chemotherapy responsiveness in TNBC cells. (biomedcentral.com)
- The antiproliferative effect of EB1089 on myeloma cells was related to the expression level of vitamin D receptor. (drlauda.at)
Eukaryotic2
- This concept incorporates autonomous cell cycle oscillators previously reported into a coherent cell cycle control by cyclin-Cdk oscillation, therefore, may have broad implications for the structure and evolution of eukaryotic cell cycle. (rockefeller.edu)
- DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
Translational1
- Many translational changes depended on PAN GU and Smaug, and these changes were largely attributable to tail-length changes. (elifesciences.org)
Tumor cells1
- The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)
Degradation1
Activity6
- This study demonstrated that modulation of PI3K/Akt/GSK3 signaling cascades can be beneficial for protecting or facilitating recovery from cellular LeTx intoxication in cells that depend on basal MEK1 activity for proliferation. (uwo.ca)
- Advances in this novel treatment strategy strongly depends on the identification of candidate miRNAs with broad-spectrum antitumor activity. (oncotarget.com)
- We have found that this activity of overexpressed Esp1 depended on spindle integrity and the MEN. (rockefeller.edu)
- In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
- We loaded gold-quercetin into poly(DL-lactide-co-glycolide) nanoparticles (NQ) and tested the biological activity of NQ on HepG2 hepatocarcinoma cells to acquire the sustained release property. (molcells.org)
- The E7 oncoprotein associates with Mi2 and histone deacetylase activity to promote cell growth. (virosin.org)
Cancer17
- One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
- In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. (neobiotechnologies.com)
- Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
- Two human breast cancer cell lines, MDA-MB-231 (wild-type BRCA1) and HCC1937 (mutated BRCA1), were compared to ascertain the difference in growth signaling depending on the presence of functional BRCA1. (snu.ac.kr)
- BRCA1 deficient breast cancer cells showed increased cell proliferation compared to breast cancer cells harboring wild-type BRCA1. (snu.ac.kr)
- Furthermore, BRCA1 deficient breast cancer cells had greater accumulation of intracellular reactive oxygen species (ROS), which was associated with a low Nrf2 mRNA level. (snu.ac.kr)
- Recent studies denoted that alterations in growth factors and growth factor receptors seem to influence the biologic behaviour of pancreatic cancer cells [ 2 ]. (biomedcentral.com)
- In turn, as a result of research into the abnormal cancer cell, the basic understanding of the cell has greatly improved. (medscape.com)
- Cancer is characterized by uncontrolled growth and division of a cell, with extension beyond the normally limiting basement membrane and through the boundaries of normal cells. (medscape.com)
- This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
- [ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
- Our studies indicated that chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis play a critical role in chemoresistance, which suggests a novel strategy to enhance chemosensitivity in breast cancer cells, especially in those of the triple-negative subtype. (biomedcentral.com)
- A cytotoxicity assay indicated that NQ preferentially killed cancer cells, compared to normal cells. (molcells.org)
- In this review, we summarize some recent findings on HPV-associated carcinogenesis, such as miRNAs in HPV-associated cancers, implication of stem cells in the biology and therapy of HPV-positive cancers, HPV vaccines, targeted therapy of cervical cancer, and drug treatment for HPV-induced intraepithelial neoplasias. (virosin.org)
- One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
- Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
- genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
Indicates that senescent1
- introduced a new concept of CKD-associated secretory phenotype (CASP), which indicates that senescent renal cells could secrete SASP components of various cytokines such as IL-1, IL-6, and TNF- α [ 19 , 20 ]. (hindawi.com)
Transcription1
- In Xenopus, zebrafish, and Drosophila the major activation of zygotic transcription occurs as the cell cycle lengthens and gastrulation begins, a developmental period referred to as the midblastula transition. (elifesciences.org)
Regulation4
- As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
- Its mechanism and regulation of its frequency by Clb2-Cdk, suggest the hypothesis that intrinsically autonomous Cdc14 release cycles are locked at once per cell cycle through entrainment by the cyclin-Cdk oscillator. (rockefeller.edu)
- Genistein caused cell cycle arrest at the G2/M phase in BRCA1 mutant cells, leading to down-regulation of Cyclin B1. (snu.ac.kr)
- The diseases where cures or at least treatments are sought are complex ones involving many potential defects in the structure, function, or regulation of the cells involved. (springeropen.com)
Mitogenic2
- It is thought that activation from the MAPK pathway provides cells with constitutive mitogenic indicators indie of extracellular stimuli (7). (molecularcircuit.com)
- This study addresses the nature of the critical labile event that couples at restriction point mitogenic cascades with the autonomous part of the cell cycle. (ac.be)
Disruption1
- In the first part of the study, the researchers exposed a group of rats to extreme stress and observed that the rats did indeed display symptoms resembling human PTSD, such as an enhanced startle reflex, impaired extinction learning and disruption of the negative feedback cycle of the stress-influenced HPA axis. (release-the-pain.com)
Human5
- In the present study, I demonstrated that a brief exposure to LeTx caused a continual MEK1 cleavage and prevented TNF-ce production in non-proliferating cells such as human peripheral blood mononuclear cells or mouse primary peritoneal macrophages. (uwo.ca)
- The CellAgeClock outperforms other epigenetic clocks in measuring subtle ageing changes in primary human cells in culture. (biorxiv.org)
- This technologic and biologic revolution continued through the 1960s to today, and the explosion in technology has fueled the current expansion of knowledge into the working of the human cell. (medscape.com)
- In the present study, we analyzed the effect of EB1089 on human myeloma cell lines. (drlauda.at)
- that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
Mechanisms2
- While the contribution of these mechanisms to cell and tissue identity is widely accepted, their role in physiological and pathological contexts within tissues is just beginning to be appreciated. (imperial.ac.uk)
- However, the precise mechanisms whereby PTN/PTPRZ1 signalling regulates the chemotherapy sensitivity of TNBC cells are not well understood. (biomedcentral.com)
Renal cell carc2
- Clinical hallmarks of VHL disease include the development of retinal and central nervous system (CNS) hemangioblastomas (blood vessel tumors), pheochromocytomas , multiple cysts in the pancreas and kidneys, and an increased risk for malignant transformation of renal cysts into renal cell carcinoma. (medscape.com)
- Early identification of VHL is important because of the increased risk of serious complications (eg, renal cell carcinoma) to foster more effective treatment options and better prognoses. (medscape.com)
Mitosis2
- In most animals the oocyte is arrested in meiosis, and fertilization leads to initiation of mitosis as the oocyte nucleus completes meiosis and fuses with the haploid sperm nucleus. (elifesciences.org)
- Required in higher cells for entry into S-phase and mitosis. (arigobio.cn)
Promotes1
- promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. (arigobio.cn)
Stimulates1
- Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
Yeast2
- Genetic control of the cell division cycle in yeast. (historyofmedicine.com)
- This event is defined by the famous cdc28 mutant (famous because the cdc28 gene was later shown to encode the founding member of the cyclin-dependent kinase family), and is also the event at which yeast mating factor arrests cell division to prepare cells for mating. (historyofmedicine.com)
Suppression1
- Recovery from cell cycle arrest was required before recovering from on-going MEK1 cleavage and suppression of TNF-a production. (uwo.ca)
Malignant3
- Misregulation of the cell cycle may result in malignant cell proliferation, tumorigenesis or cell death. (intechopen.com)
- Biopsy specimens of the stomach showed a dense, diffuse infiltrate of malignant plasma cells in the lamina propria of fundus and corpus. (drlauda.at)
- In our patient the vitamin B12 deficiency may be related to a displacement or destruction of parietal cells by malignant plasma cells. (drlauda.at)
Oncogenic2
- These studies have got described the molecular occasions inside the signaling cascade that result in premature senescence and therefore have provided brand-new insights into how confers oncogenic change in major cells. (molecularcircuit.com)
- Oddly enough the AP24534 power of oncogenic to induce premature senescence depends upon the Raf-MEK-ERK pathway that mediates cell proliferation (36). (molecularcircuit.com)
Phenotype1
- Overexpression of Cmb causes the formation of a multiple hair cell phenotype (MHC), similar to loss of rok and mwh. (uni-wuerzburg.de)
Growth5
- It has been reported that under certain biological conditions p38 AP24534 can negatively AP24534 regulate cell growth. (molecularcircuit.com)
- Briefly, for testing cell growth in soft agar, 103 cells dissociated from neurospheres were suspended in 3 ml Adv DME containing 5% FBS and 0. (plksignaling.com)
- In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
- I examined the effects of genistein on proliferation and growth of TNBC cells, and how these effects differ depending on the presence or absence of functionally active BRCA1. (snu.ac.kr)
- Because of its mutated aggressive genetics, this cell has a selective growth advantage over its neighbors. (medscape.com)