Cyclin d1 binds cyclin dependent kinase inhibitor p27. Medical search. Frequent questions

p27(Kip1) is a cyclin-dependent kinase inhibitor. (biomedcentral.com)
The cyclin-dependent kinase (CDK) inhibitor p27(Kip1) is a key cell-cycle regulator of G1-to-S phase transition [ 1 ]. (biomedcentral.com)
ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
PD-1 suppressed the transcription of SKP2 , the substrate recognition component of the SCF Skp2 ubiquitin ligase that leads p27 kip1 to degradation and resulted in accumulation of p27 kip1 (Figure 2). (shu.edu)
What are the effects of p27 kip1 and p15 INK4 ? (shu.edu)
AKT prevents cell death by inactivating proapoptotic factors including BAD, procaspase-9 and Forkhead transcription factor family proteins (FOXOs), activates transcription factors that upregulate antiapoptotic genes, including NF-kB, inactivates p53 through Mdm2, and phosphorylates the cell cycle inhibitors p21 CIP1/WAF1 and p27 KIP1 , thus increasing cell proliferation (Hennessy et al. (ebrary.net)
Cell cycle progression is faster in cells in which invadopodia are abolished (by Tks5 knockdown), evidenced by earlier induction of cyclins A and B. A close look at the regulators of G1 revealed that the overexpression of p27 kip1 , but not p21 cip1 , causes faster turnover of invadopodia and increased ECM degradation. (biorxiv.org)
Furthermore, both endogenous and over-expressed p27 kip1 localizes to the sites of invadopodia assembly. (biorxiv.org)
In recent years, cell cycle regulators have been shown to exhibit roles in both tumor suppression and tumor promotion, particularly cyclin-dependent kinase inhibitors (CKI) p27 kip1 and p21 cip1 14 . (biorxiv.org)

This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (wikipedia.org)
Cyclin D1 is a regulatory subunit of cyclin-dependent kinases CDK4 and CDK6. (wikipedia.org)
The cyclin D1-CDK4 complex promotes passage through the G1 phase by inhibiting the retinoblastoma protein (pRb). (wikipedia.org)
Cyclin D1-CDK4 inhibits pRb through phosphorylation, allowing E2F transcription factors to transcribe genes required for entry into the S phase. (wikipedia.org)
Cyclin D1-CDK4 also enables the activation of cyclin E-CDK2 complex by sequestering Cip/Kip family CDK inhibitory proteins p21 and p27, allowing entry into the S phase. (wikipedia.org)
Cyclin D1-CDK4 also associates with several transcription factors and transcriptional co-regulators. (wikipedia.org)
Under some circumstances, p27 is associated with active CDK4, but no mechanism for activation has been described. (rcsb.org)
We found that p27, when phosphorylated by tyrosine kinases, allosterically activated CDK4 in complex with cyclin D1 (CDK4-CycD1). (rcsb.org)
Structural and biochemical data revealed that binding of phosphorylated p27 (phosp27) to CDK4 altered the kinase adenosine triphosphate site to promote phosphorylation of the retinoblastoma tumor suppressor protein (Rb) and other substrates. (rcsb.org)
Our data characterize phosp27-CDK4-CycD1 as an active Rb kinase that is refractory to clinically relevant CDK4/6 inhibitors. (rcsb.org)
Analysis of G(1) regulatory proteins demonstrated that protein levels of CDK2, CDK4, cyclin D1, and cyclin A were decreased in a time-dependent manner, but not those of CDK6 and cyclin E, by EB1089. (drlauda.at)
These events resulted in upregulation of the Cdk4/6 inhibitor p15 INK4B and repression of the Cdk-activating phosphatase Cdc25A. (shu.edu)
P15 INK4 is a cyclin dependent kinase inhibitor that blocks the activity of Cyclind-CDK4,6, inhibiting it from hypophosphorylating Rb, thereby, rendering the cell cycle unresponsive to external proliferation signals. (shu.edu)
Full holoenzyme activity of the cyclin D1-Cdk4 complex is induced by mitogen recruitment of CAK. (shu.edu)
The cyclin D1-Cdk4 complex phosphorylates the pRB protein leading to sequential phosphorylation by cyclin E-Cdk2 and release of free E2F. (shu.edu)
This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)
CDK4 is a member of the Ser/Thr protein kinase family. (thermofisher.com)

Two proteins have been implicated in expression-independent vitamin D action: membrane VDR and the membrane-associated rapid response steroid binding (1,25D 3 -MARRS) protein. (biomedcentral.com)
The effects of DNA methylation and the histone code are due, at least in part, to modification-specific recruitment of factors, such as heterochromatin-associated proteins (HP1) and methyl-binding domain proteins, which establish and maintain higher order of chromatin structure. (aacrjournals.org)
Through molecular docking, the binding mode between inhibitors and proteins was elucidated. (bvsalud.org)
Ethyl 2-((2 3 bis(nitrooxy)propyl)disulfanyl)benzoate (GT-094) a novel nitro-NSAID induced ROS and decreased mitochondrial membrane potential (MMP) in SW480 and RKO colon cancer cells and treatment with antioxidants GSH and DTT inhibited ROS generation prevented the loss of MMP and reversed the effects on downregulation of Sp1 Sp3 and Sp4 proteins and Sp dependent genes [42]. (tam-receptor.com)
Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)
We found that LCH decreased epidermal growth factor receptor (EGFR) and AKT kinase activities and related activating signaling proteins including pEGFR and pAKT. (biomolther.org)
Proteins which bind to DNA. (lookformedical.com)
The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
Proteins that specifically bind to TELOMERES. (lookformedical.com)
It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. (cancerindex.org)
Invadopodia are small membrane protrusions, rich in actin and actin-binding proteins, such as cortactin and Tks5 3 - 8 . (biorxiv.org)
Rho GTPases represent a family of small GTP-binding proteins involved in cell cytoskeleton organization, migration, transcription, and proliferation. (biomedcentral.com)
All members contain the sequence motifs characteristic of all GTP-binding proteins, bind to GDP and GTP with high affinity. (biomedcentral.com)
In turn, GTP-bound active GTPases can interact with a plethora of different effectors which mediate the different cellular functions of this family of proteins. (biomedcentral.com)
Rho GTPase effectors are a large group of proteins and include actin nucleation promoting molecules, adaptors, as well as kinases. (biomedcentral.com)

The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. (cancerindex.org)

Independent of CDK, cyclin D1 binds to nuclear receptors (including estrogen receptor α, thyroid hormone receptor, PPARγ and AR ) to regulate cell proliferation, growth, and differentiation. (wikipedia.org)
b) 4-Hydroxytamoxifen (but not tamoxifen), genistein (but not genistin), daidzein, and probably other nutritional and chemopreventive anti-cancer agents could up-regulate expression of p27 via receptor protein tyrosine kinases (RPTKs), phosphoinositide 3-kinase (PI3K), phosphoinosite-dependent kinase (PDK), Akt/PKB and mTOR. (biomedcentral.com)
In addition, TRO can cause an induction of Cu 2+ /Zn 2+ -superoxide dismutase (CuZnSOD) [ 20 , 21 ] and the activated receptor complexes of TRO can induce catalase through binding to its promoter region [ 22 , 23 ]. (e-roj.org)
Growth factors that signal through tyrosine-kinase receptor families include the epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and transforming-growth factor-α (TGF-α). (janechin.net)
Transforming growth factor-β1 (TGF-β) signals through a serine/threonine-kinase receptor pathway. (janechin.net)
Vitamin D is a steroid hormone that exerts most of its biological activities by binding to a specific high-affinity receptor, the vitamin D receptor (VDR). (biomedcentral.com)
Receptor-regulated SMADs (R-SMADs), SMAD1, 2, 3, 5, and 8, are the only SMADs directly phosphorylated and activated by the kinase domain of type I receptors. (shu.edu)
Small-molecule inhibitors, antagonistic monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), bispecific antibodies or biologics (bsAbs) and chimeric antigen receptor-modified T cells (CAR-Ts) targeting Notch signaling components have been developed as investigational anti-cancer drugs ( 10 - 12 ). (spandidos-publications.com)
Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. (researchgate.net)
miR-128-5p targets the tissue inhibitor of metalloproteinases (TIMPs), silent information regulator 2 (SIRT2), peroxisome proliferator-activated receptor (PPAR), and p21 genes, which are linked to the behaviours of VSMCs. (hindawi.com)
Although neither kinase-dead human epidermal growth factor receptor (HER)3 nor orphan HER2 can be activated by HER-related ligands on their own, the formation of HER2/HER3 heterodimers creates the most mitogenic and transforming receptor complex within the HER (erbB) family of transmembrane receptor tyrosine kinases. (biomedcentral.com)
However, it appears that regardless of the mechanism that triggers the formation of oncogenic HER2/HER3 heterodimers (HER2 over-expression or overall low HER2 but high levels of the HER3 ligand heregulin), HER3 transphosphorylation is a common response of breast cancer cells upon treatment with current inhibitors of the HER receptor tyrosine kinase network. (biomedcentral.com)
The notion that breast cancer disease can be viewed as a biological process that is driven by overactive human epidermal growth factor receptor (HER)1/2 receptor tyrosine kinases (RTKs) has led to development of various anti-HER tyrosine kinase agents. (biomedcentral.com)

HDAC inhibition resulted in a transcriptional and posttranscriptional regulation of the cyclin-dependent kinase inhibitors p21(Cip1) and p27(Kip). (cret-signal.com)

Cyclins function as regulators of CDKs (cyclin-dependent kinase). (wikipedia.org)
The p27 protein is a canonical negative regulator of cell proliferation and acts primarily by inhibiting cyclin-dependent kinases (CDKs). (rcsb.org)

Transcriptional and translational control, sequestration in cyclin D1 complexes and localization all regulate p27 in G1 phase. (biomedcentral.com)

Cyclin D1 overexpression can also down-regulate Fas expression, leading to increased chemotherapeutic resistance and protection from apoptosis. (wikipedia.org)
In addition, cell cycle distribution analysis revealed that G0/G1 phase arrest was induced following pristimerin treatment in CAL‑27 and SCC‑25 cells, which was strongly associated with upregulation of p21 and p27, coupled with downregulation of cyclin D1 and cyclin E. Meanwhile, pristimerin induced significant apoptosis of CAL‑27 and SCC‑25 cells, alongside decreased levels of caspase‑3 and specific cleavage of poly (ADP‑ribose) polymerase. (spandidos-publications.com)

When up-regulated, p27 inhibits G1-to-S phase transition of the cell cycle. (biomedcentral.com)
2005). AKT also phosphorylates and inhibits glycogen synthase kinase-3 (GSK3), thus enhancing p-catenin and cyclin D1 stabilization (Vivanco and Sawyers 2002). (ebrary.net)

Cyclin D1 overexpression has been shown to correlate with early cancer onset and tumor progression and it can lead to oncogenesis by increasing anchorage-independent growth and angiogenesis via VEGF production. (wikipedia.org)
Anti-PD-1 antibodies (dark green) or anti-PD-L1 antibodies (light green) can prevent the tumor cell from binding PD-1 and thus allow T cells to remain active. (shu.edu)
The Notch signaling network exerts oncogenic and tumor‑suppressive effects in a cancer stage‑ or (sub)type‑dependent manner. (spandidos-publications.com)
BCRC-3 functions as a tumor inhibitor to suppress BC cell proliferation through miR-182-5p/p27 axis, which would be a novel target for BC therapy. (biomedcentral.com)

The class I PI3Ks are activated by growth factor tyrosine kinase receptors (class IA), such as EGFR, or by G protein coupled receptors (GPCRs) (class IB). (ebrary.net)
Also, the growth-inhibitory effects of HER1/2 tyrosine kinase inhibitors (TKIs) were previously found to be attenuated in the presence of heregulin, which is a high-affinity combinatorial ligand for HER3. (biomedcentral.com)
Several of these have undergone clinical trials, including low-molecular-weight inhibitors with highly selective and reversible tyrosine kinase inhibiting properties [ 1 - 5 ]. (biomedcentral.com)

Cyclin D1 overexpression is correlated with shorter cancer patient survival and increased metastasis. (wikipedia.org)
Amplification of the CCND1 gene is present in: non-small cell lung cancers (30-46%) head and neck squamous cell carcinomas (30-50%) pancreatic carcinomas (25%) bladder cancer (15%) pituitary adenomas (49-54%) breast carcinoma (13%) Cyclin D1 overexpression is strongly correlated to ER+ breast cancer and deregulation of cyclin D1 is associated with hormone therapy resistance in breast cancer. (wikipedia.org)
Mechanistically, overexpression of BCRC-3 induced the expression of cyclin-dependent kinase inhibitor 1B (p27). (biomedcentral.com)

Subsequently, mTOR phosphorylates key eukaryotic translation regulators, including p70-S6 kinase (p70S6K) and the eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1) (Hay and Sonenberg 2004). (ebrary.net)

Amino acid deficiencies also up-regulate the expression of p27 using some components of this pathway. (biomedcentral.com)
Growth factors do not only stimulate cell proliferation, but they may also act as growth inhibitors, depending on the cell type and the stimulatory pathway that is involved. (biomedcentral.com)
This pathway involves the sequential activation of Phosphoinositide 3-kinase (PI3K), AKT, and mTOR. (ebrary.net)
RAD001 (everolimus), a mammalian target of rapamycin (mTOR) pathway inhibitor in phase II clinical trials in oncology, exerts potent antiproliferative/antitumor activities. (aacrjournals.org)
Their regulation may be carried out either through direct binding to DNA as peroxisome proliferator-activated receptors or via modulation in an indirect manner of signaling pathway molecules (e.g., protein kinase C) and other transcription factors (nuclear factor kappa B and sterol regulatory element binding protein). (springer.com)
Although it is kinase defective, the HER family member HER3 can be phosphorylated by HER1 or HER2, and HER3 can couple with the pro-survival phosphatidylinositol-3-OH kinase (PI[ 3 ]K)/Akt pathway directly whereas HER1 and HER2 cannot [ 5 ] (Figure 1 ). (biomedcentral.com)

This was accompanied by increased manifestation of cyclin-dependent kinase inhibitor p27 with Sp3 knockdown [54-56]. (tam-receptor.com)
In addition, TGF-β1 induced FOXK1 expression in a time-dependent pattern and the knockdown of FOXK1 inhibited TGF-β1-induced EMT. (oncotarget.com)

Increasing cyclin D1 levels during the G1 phase is induced by mitogenic growth factors primarily through Ras-mediated pathways, and hormones. (wikipedia.org)
These Ras-mediated pathways lead to the increase in transcription of cyclin D1, and inhibit its proteolysis and export from the nucleus. (wikipedia.org)
These effects were associated with inhibition of the mitogen‑activated protein kinase/extracellular signal‑regulated kinase 1/2 and protein kinase B signaling pathways. (spandidos-publications.com)
The underlying mechanisms of these effects were primarily mediated by G 1 phase cell cycle arrest and inhibition of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulate kinase 1/2 (Erk 1/2) and protein kinase B (Akt) signaling pathways. (spandidos-publications.com)

Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (wikipedia.org)
This apparent increase in p27 protein expression might have been due to either increased synthesis or decreased degradation, or a combination of both [ 1 ]. (biomedcentral.com)
Mechanically, DNAJA1 could bind with P53-R175H and reduced its degradation. (bvsalud.org)

A computational docking model indicated that LCH may interact with EGFR, AKT1, and AKT2 at the ATP-binding sites. (biomolther.org)

Activated complexes accumulate in the nucleus where they cooperate with DNA-binding cofactors to regulate target gene transcription. (shu.edu)

Cyclin D1 also binds to histone acetylases and histone deacetylases to regulate cell proliferation and cell differentiation genes in the early to mid-G1 phase. (wikipedia.org)
We also aimed to evaluate the expression of TGF-β1 and p27 in the context of other cell cycle and proliferation markers such as cyclin D1 and Ki-67. (biomedcentral.com)
EB1089 inhibited the proliferation of NCI-H929 cells and RPMI8226 cells in a dose-dependent manner among three myeloma cell lines tested. (drlauda.at)
Many breast cancers are dependent for proliferation on estrogens synthesized from androgens (i.e., androstenedione) by aromatase. (aacrjournals.org)
VSMCs were transfected with the miR-128-5p mimic and inhibitor, and the migration, phenotypic modulation, and proliferation of VSMCs were investigated. (hindawi.com)
AA and the extracellular regulated protein kinase 1/2 (ERK1/2) blocker U0126 markedly inhibited migration, elevated smooth muscle 22 α (SM22 α ) expression, repressed VSMC proliferation, elevated miR-466f-3p and miR-425-3p expression, and suppressed miR-27a-5p and miR-128-5p expression in ox-LDL-induced VSMCs. (hindawi.com)
The miR-128-5p mimic promoted the migration and proliferation of VSMCs and suppressed p21, p27, and SM22ɑ expression. (hindawi.com)
The inhibitor increased p21, p27, and SM22ɑ expression and repressed the migration, phenotypic transformation, and proliferation of VSMCs. (hindawi.com)
miR-128-5p directly targeted the 3′UTR-binding sequences of the p21 gene, negatively regulated p21 expression, and supported the proliferation of VSMCs. (hindawi.com)
The migration, phenotypic transformation, and proliferation of VSMCs lead to vascular wall remodeling, which is mediated by activation of extracellular regulated protein kinases 1/2 (ERK1/2) signalling [ 9 ]. (hindawi.com)
Several studies have shown that miRNAs play multiple roles in the phenotypic transformation, migration, and proliferation of VSMCs by inhibiting ERK1/2 activation [ 11 , 12 ], partly by regulating the tissue inhibitor of metalloproteinases (TIMPs)-MMPs and p21-cyclins interactions [ 13 - 18 ]. (hindawi.com)

This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)

Signaling specificity is conferred by receptors and mediated through associated-kinases. (janechin.net)
TGF-β ligands bind type I and type II receptors at the cell surface. (shu.edu)
Small‑molecule γ‑secretase inhibitors (AL101, MRK‑560, nirogacestat and others) and antibody‑based biologics targeting Notch ligands or receptors [ABT‑165, AMG 119, rovalpituzumab tesirine (Rova‑T) and others] have been developed as investigational drugs. (spandidos-publications.com)
These pericryptal myofibroblasts produce the Wnt signalling ligands which bind to Frizzled receptors as well as morphogenetic protein (BMP) antagonists gremlin 1(GREM1), gremlin 2 (GREM2) on basal epithelial stem cells and also can modulate notch signaling. (biomedcentral.com)

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. (wikipedia.org)
Sp Transcription Factors Members of the Sp/KLF family have a highly conserved DNA binding website that consists of three contiguously placed C2H2-type zinc fingers that are located in the C-terminal region. (tam-receptor.com)

Cyclin D1 is degraded by the proteasome upon phosphorylation of threonine 286 and subsequent ubiquitylation via the CRL4-AMBRA1 E3 ubiquitin ligase complex. (wikipedia.org)

Cyclin D1 is a protein that in humans is encoded by the CCND1 gene. (wikipedia.org)
The CCND1 gene encodes the cyclin D1 protein. (wikipedia.org)
Up-regulation of the expression of p27 is likely due to the activation of translation rather than transcription of p27 because (a) up-regulation is mediated by the 5'-untranslated region (-575) of the p27 gene and (b) the antibiotic actinomycin D, an inhibitor of transcription, did not attenuate the up-regulation of p27. (biomedcentral.com)
This latter finding is likely to preclude the existence of cryptic transcription factor binding site(s) in the 5'-untranslated region of p27 gene. (biomedcentral.com)
The experimental evidence, presented in the second half of this report, was obtained using the 5'-untranslated region (-575) of p27 gene. (biomedcentral.com)
To address this question, the effects of various nutritional and chemopreventive anti-cancer agents on the activity of the proximal 5'-upstream region of p27 gene were investigated by transient transfection assay. (biomedcentral.com)
Vitamin D is also known to exert rapid effects that are not dependent on gene transcription [ 5 ]. (biomedcentral.com)
Members of the family bind to GC boxes (GGGGCGGGG) GT/CACCC Semagacestat boxes (GGTGTGGGG) and fundamental transcription elements to regulate gene transcription [44-47]. (tam-receptor.com)
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)
This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). (thermofisher.com)
The 3′UTR-binding sequence site of miR-128-5p on the p21 gene was predicted and assessed by luciferase assays. (hindawi.com)
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. (cancerindex.org)
This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. (cancerindex.org)

cyclin D1 mRNA expression and cyclin D1 promoter activity. (cret-signal.com)

PMID: 10102234 [PubMed - indexed for MEDLINE] 1: Exp Cell Res 2000 Feb 1;254(2):279-86 Cell cycle arrest induced by the vitamin D(3) analog EB1089 in NCI-H929 myeloma cells is associated with induction of the cyclin-dependent kinase inhibitor p27. (drlauda.at)

Cyclin D1 was originally cloned as a breakpoint rearrangement in parathyroid adenoma and was shown to be required for progression through the G1 phase of the cell cycle to induce cell migration, angiogenesis and to induce the Warburg effect. (wikipedia.org)

Moreover, PD-1 and PD-L1 inhibitors are being tested in combination with other checkpoint inhibitors, targeted therapies, cancer vaccines, monoclonal antibodies, and other modalities. (shu.edu)

This growth inhibition by TRO is linked to the G1 phase cell cycle arrest through the up-regulation of the cyclin-dependent kinase inhibitors p21 and p27 [ 14 - 16 ] and/or repression of cyclin D1 expression [ 17 , 18 ]. (e-roj.org)

p27 expression is reduced in pancreatic adenocarcinomas and decreased protein levels of p27 may play a role in the differentiation of pancreatic cancer. (biomedcentral.com)

AKT phosphorylation and inactivation of TSC2 results in increased levels of the GTP-bound (active) form of Rheb1, which in turn promotes the phosphorylation and activation of mTOR, also known as the mammalian target of rapamycin (Manning and Cantley 2003). (ebrary.net)

Of the 63 tumors evaluated 23 [36.5%] were positive for p27 within the nucleus. (biomedcentral.com)
Accumulation of p27 in the nucleus, therefore, blocks cell cycle progression of T-lymphocytes that are being induced to act against cancer antigens. (shu.edu)

Finally, we provide evidence that these observations are applicable in vivo by demonstrating that HDAC inhibition decreased neointima formation and expression of cyclin D1 in a murine model of vascular injury. (cret-signal.com)
In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)
in particular, HER3 signalling buffered against incomplete inhibition of HER2 kinase activity has been suggested to be the mechanism that allows HER2 over-expressing breast cancer cells to escape HER TKIs. (biomedcentral.com)
Failure to reverse breast cancer progression despite apparent inhibition of HER1 and HER2 kinase functions in cell-based assays as well as in patient tissues and tumours is an enigma that is not yet resolved [ 6 ]. (biomedcentral.com)

Up-regulation appears to be specific to p27 because expression of cyclin D1, E, and A, and p21Cip1/Waf1 was not modulated by these agents. (biomedcentral.com)

Pioneering studies showed that high expression of kinase-dead HER3 can predict early escape from the anti-HER2 monoclonal antibody trastuzumab. (biomedcentral.com)

This report addresses the question of whether various nutritional and chemopreventive anti-cancer agents up-regulate the expression of p27 in preneoplastic and neoplastic cells. (biomedcentral.com)
Experimental evidence presented in the first half of this report shows that these agents fairly faithfully up-regulate expression of p27 in mouse epidermal (JB6) and human breast cancer (MCF7, MDA-MB-321, and AU565) cells. (biomedcentral.com)
Finally, (d) global hypomethylation of 5'-m 7 G cap of mRNAs could also up-regulate expression of p27. (biomedcentral.com)
Based on these findings, we conclude that various nutritional and chemopreventive anti-cancer agents up-regulate expression of p27 in (pre)neoplastic cells. (biomedcentral.com)
CiRS-7 has more than 70 miR-7 binding sites and thus acts as effective miR-7 suppressor to regulate the expression of miR-7 target mRNAs. (biomedcentral.com)

Canonical Notch signaling activates the transcription of BMI1 proto‑oncogene polycomb ring finger, cyclin D1, CD44, cyclin dependent kinase inhibitor 1A, hes family bHLH transcription factor 1, hes related family bHLH transcription factor with YRPW motif 1, MYC, NOTCH3, RE1 silencing transcription factor and transcription factor 7 in a cellular context‑dependent manner, while non‑canonical Notch signaling activates NF‑κB and Rac family small GTPase 1. (spandidos-publications.com)
Here, we demonstrate that the endoplasmic reticulum stress sensor inositol-requiring enzyme 1 (IRE1α) and its substrate transcription factor X-box-binding protein 1 (XBP1) drive NK cell responses against viral infection and tumors in vivo. (cancerindex.org)

Immunohistochemical staining of cyclin D1 antibodies is used to diagnose mantle cell lymphoma. (wikipedia.org)
The purpose of our study was to investigate the immunohistochemical expression of TGF-β1 and p27 in pancreatic adenocarcinomas and to compare the findings with the clinicopathological features and survival. (biomedcentral.com)

Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells. (pharmaceuticalintelligence.com)

p27 is a cyclin dependent kinase inhibitor that blocks the activity of Cyclin E-CDK2, which phosphorylates pRb, thereby ushering the cell from G1 into S phase through the Restriction point (Figure 2). (shu.edu)

Bortezomib restores expression of FoxO3a in imatinib resistant T315I cells The T315I BCR ABL mutation frequently occurs in CML patients that are resistant to imatinib, and even remains refractory to the more potent second generation kinase inhibitors. (mi-rna.com)

Cyclin D1 is a protein required for progression through the G1 phase of the cell cycle. (wikipedia.org)
Cell cycle progression is governed by a complex network of cyclin-dependent kinases that define not only the phase of the cell cycle, but also the timing of transitions between phases 13 . (biorxiv.org)
Transphosphorylation of kinase-dead HER3 and breast cancer progression: a new standpoint or an old concept revisited? (biomedcentral.com)

This study provided evidence that the up-regulation of p27 protein expression is at least in part due to increased synthesis and that this increase fairly faithfully recapitulates the cancer preventive activity of nutritional and chemopreventive anti-cancer agents. (biomedcentral.com)
Further studies were conducted to gain some insight into the molecular basis of this increase in the synthesis of p27. (biomedcentral.com)

It has also been demonstrated that TGF-β induced cell cycle arrest can be partially attributed to the regulatory effects of TGF-β on both the expression and activity of cyclin-dependent kinase inhibitors [CDKI] such as p21 and p27. (biomedcentral.com)
The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). (thermofisher.com)
Importantly, BCRC-3 could directly interact with miR-182-5p, and subsequently act as a miRNA sponge to promote the miR-182-5p-targeted 3'UTR activity of p27. (biomedcentral.com)

c) Expression of p27 could also be up-regulated via RPTKs followed by MAPKs - MEK, ERK and p38MAPK - and probably MNK. (biomedcentral.com)
Preliminary studies using either N -methyl- N -nitrosourea (MNU)-induced rat breast cancer model or human breast cancer cell lines in vitro had suggested, but not proved, that nutritional and chemopreventive anti-cancer agents increase p27 protein expression. (biomedcentral.com)
Preliminary studies using in vivo model of MNU-induced rat mammary cancer and in vitro model of cultured cells had suggested - but not proved - that various nutritional and chemopreventive anti-cancer agents, including moderate dietary restriction, up-regulated the expression of p27 (Fig. 1a ). (biomedcentral.com)
The change of radiation sensitivity by combined with TRO is not dependent on the PPARγ expression level. (e-roj.org)
We examined TGF-β1 and p27 expression immunohistochemically in 63 cases of invasive ductal adenocarcinoma of the pancreas. (biomedcentral.com)
In the univariate analysis, neither TGF-β1 nor p27 expression was related with patient survival. (biomedcentral.com)

It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 2 in that it contains acidic N-terminal amino acid residues. (lookformedical.com)

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. (cellsignal.com)

They are small (21-25 kDa) molecules that share structural homology and become activated only when bound to GTP. (biomedcentral.com)

Anatomy8

Organisms2

Diseases2

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes37

Disciplines and Occupations1

Information Science3

Kip19

CDK417

Proteins15

Including D-type cyclins1

Receptor13

Cip11

CDKs2

Transcriptional1

Apoptosis2

Inhibits2

Tumor4

Tyrosine-kinase3

Overexpression3

Regulators1

Pathway6

Knockdown2

Pathways4

Degradation3

EGFR1

Complexes1

Proliferation11

Subunit1

Receptors4

Highly conserved2

Ubiquitin ligase1

Gene13

Promoter1

Induction1

Angiogenesis1

Monoclonal antibodies1

Repression1

Protein levels1

Phosphorylation1

Nucleus2

Inhibition4

Waf11

HER21

Regulate5

Transcription2

Immunohistochemical2

AMPK1

Phosphorylates1

Refractory1

Progression3

Synthesis2

Activity3

Expression6

Amino1

Complex1

Structural1