Cyclin A degradation employs preferentially used lysines and a cyclin box function other than Cdk1 binding. (nih.gov)
The model was built by integrating and modifying two previously published models such that the model outputs for cyclins A and B fit cyclin expression measurements and the activation of B cyclin/Cdk1 coincided with phosphorylation of histone H3. (ebi.ac.uk)
The model depends on Cdh1-regulated cyclin degradation during G1, regulation of B cyclin/Cdk1 activity by cyclin A/Cdk via Wee1, and transcriptional control of the mitotic cyclins that reflects some of the current literature. (ebi.ac.uk)
This is in contrast to control cells where cyclin B 1 appears at the centrosomes in early prophase based on cell cycle-specific localization of CENP-F. Furthermore, cyclin B/Cdk1 kinase activity in early G 2 is aberrantly high in BubR1-depleted cells. (ewha.ac.kr)
Therefore, this study explains how corona-MAD1 generates a robust SAC signal, and it reveals a scaffolding role for the key mitotic kinase, Cyclin B1:CDK1, which ultimately helps to inhibit its own degradation. (mpg.de)
CCNB12
Orthologous to several human genes including CCNB1 (cyclin B1). (nih.gov)
These paclitaxel resistant ovarian cells demonstrate: (1) Increased IC 50 for paclitaxel and docetaxel (10 to 75-fold) and cross-resistance to anthracyclines (2) Reduced cell apoptosis in the presence of paclitaxel (3) Gene depletion involving mitotic regulators BUB1 mitotic checkpoint serine/threonine kinase, cyclin BI (CCNB1), centromere protein E (CENPE), and centromere protein F (CENPF), and (4) Functional data validating gene depletion among mitotic regulators. (biomedcentral.com)
Phosphorylation3
In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. (nsjbio.com)
Studies with Mcl-1 phosphorylation mutants show that cyclin E/Cdk2-dependent phosphorylation of Mcl-1 residues on its PEST domain resulted in increased Mcl-1 stability (Thr92, and Thr163) and Bim binding (Ser64). (oncotarget.com)
Infection of liver tumor cells with AdCN305 SOCS3 and AdCN305 cpp SOCS3 resulted in almost complete inhibi tion of STAT3 phosphorylation and downregulation of cyclin D1 and Bcl xL. (micrornaarray.com)
Mitosis4
This is characterized by high levels of cyclin B1 still present in the cell at the time of cell death indicating the cell never finished mitosis. (wikipedia.org)
We introduced autocatalytic transcription of E2F, E2F regulated transcription of cyclin B, Cdc20/Cdh1 mediated E2F degradation, enhanced transcription of mitotic cyclins during late S/early G2 phase, and the sustained synthesis of cyclin B during mitosis. (ebi.ac.uk)
This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. (nsjbio.com)
Activation of the Cyclin B/Cdc2 kinase complex triggers entry into mitosis in all eukaryotic cells. (duke.edu)
Kinase activity1
Cyclin E/Cdk2 kinase activity is frequently deregulated in human cancers, resulting in impaired apoptosis. (oncotarget.com)
Complexes3
Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1. (duke.edu)
Consequently, most cyclin-dependent kinase (Cdk)/cyclin complexes are localized to the nucleus when active. (duke.edu)
This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. (novusbio.com)
Cdk22
Here, we show that cyclin E/Cdk2 phosphorylates and stabilizes the pro-survival Bcl-2 family protein Mcl-1, a key cell death resistance determinant to the small molecule Bcl-2 family inhibitors ABT-199 and ABT-737, mimetics of the Bcl-2 homology domain 3 (BH3). (oncotarget.com)
Cyclin E levels were elevated and there was increased association of cyclin E/Cdk2 with Mcl-1 in ABT-737-resistant compared to parental cells. (oncotarget.com)
Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. (novusbio.com)
Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - CCNH monoclonal antibody (M01), clone 1B8 Analysis of CCNH expression in HeLa. (novusbio.com)
This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. (nsjbio.com)
Hamster protein was used as the immunogen for this Cyclin B1 antibody. (nsjbio.com)
Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide). (nsjbio.com)
Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. (novusbio.com)
Sandwich ELISA: Cyclin H Antibody (1B8) [H00000902-M01] - Detection limit for recombinant GST tagged CCNH is approximately 1ng/ml as a capture antibody. (novusbio.com)
Protein7
Enables cyclin-dependent protein serine/threonine kinase regulator activity. (nih.gov)
The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. (nsjbio.com)
This negative regulation of DNA-PKcs by depleting PIG3 seemed to take place at the translational level but not at the levels of transcription or protein degradation. (ijbs.com)
Cyclin E depletion in various human tumor cell-lines and cyclin E -/- mouse embryo fibroblasts showed decreased levels of Mcl-1 protein, with no change in Mcl-1 mRNA levels. (oncotarget.com)
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (novusbio.com)
This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. (novusbio.com)
P34cdc24
Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
We then studied the effect of Dox on the p34cdc2/cyclin B1 complex because it plays a key role in regulating G2/M phase transition. (aspetjournals.org)
Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
Cyclin B1-p34cdc2 plays a critical role in G2 to M transition. (nsjbio.com)
Specificity1
Tissue specificity of cyclin B expression in D. melanogaster]. (nih.gov)
Transition1
This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. (nsjbio.com)
Depletion2
As expected, BubR1 depletion leads to degradation of cyclin B 1 in the G 2 phase. (ewha.ac.kr)
Together, our results indicate that hBubR1 depletion triggers premature centrosomal localization of cyclin B 1 probably leading to premature mitotic entry. (ewha.ac.kr)
Localization1
Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1. (duke.edu)
Regulation1
Collectively, our findings identify a novel mechanism of cyclin E-mediated Mcl-1 regulation that provides a rationale for clinical use of Bcl-2 family and Cdk inhibitors for Mcl-1-dependent tumors. (oncotarget.com)
Cytoplasm1
Cyclin B Export to the Cytoplasm via the Nup62 Subcomplex and Subsequent Rapid Nuclear Import Are Required for the Initiation of Drosophila Male Meiosis. (nih.gov)
Accumulation2
In contrast, Dox treatment was found to induced cyclin B1 accumulation as a result of stimulating its synthesis and inhibiting its degradation. (aspetjournals.org)
MDA-MB-435 cells responded to silvestrol treatment with accumulation of LC3-II and time-dependent p62 degradation. (biomedcentral.com)
Synthesis1
The mechanism, which is based on the synthesis and degradation of three ''master regulator'' proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. (ebi.ac.uk)
Regulators1
Cyclins function as regulators of CDK kinases. (novusbio.com)
Distinct1
Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (novusbio.com)
Stability1
The group of Adrian Saurin from the University of Dundee together with the group of Andrea Musacchio show here that, in addition to its kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help preserve genome stability. (mpg.de)
Proteins1
INTRODUCTION: Proteins that undergo receptor-mediated endocytosis are subject to lysosomal degradation, requiring radioiodination methods that minimize loss of radioactivity from tumor cells after this process occurs. (duke.edu)
By 24 h, a decrease in cyclin B and cyclin D expression was observed in silvestrol-treated cells relative to control. (biomedcentral.com)
Activity2
Then, the discussion about the photocatalytic activity of the AgNPs (either in suspension or impregnation), and correlation with your properties and its potential application to organic pollutants degradation under UV and visible/solar radiation will be described. (intechopen.com)
Robustness arises because Cyclin B1:MAD1 localisation loses dependence on MPS1 kinase after the corona has been established, ensuring that corona-localised MAD1 can still be phosphorylated when MPS1 activity is low. (mpg.de)
Levels1
Intriguingly, cyclin B 1 is prematurely targeted to centrosomes appearing at early G 2 phase in BubR1-depleted cells despite its low levels. (ewha.ac.kr)
Cells1
Cyclin E knock-down restored ABT-737 sensitivity to acquired and inherently resistant Mcl-1-dependent tumor cells. (oncotarget.com)
Assembly1
Cyclin B1 localises to an expanded region of the outer kinetochore, known as the corona, where it scaffolds the spindle assembly checkpoint (SAC) machinery by binding directly to MAD1. (mpg.de)
Role1
This study is the first to suggest a role of hBubR1 in controlling centrosome targeting of cyclin B 1 and timing of mitotic entry. (ewha.ac.kr)