ImmunogenProteinPolyclonalIsotypeKinasesReactivityProteinsAntigenDatasheetPhosphorylationSpecificityMonoclonal antibodiesWestern BlotLysateKinase inhibitorELISASecondary AntibodyCustom antibody servicesCDC2CCND1HeLaCloneCdksSpeciesGeneAzideOverexpressionConcentrationTumorLymphomaSubstrateSubunitsFitcAffinityDependentPeptideMitoticImmunohistochemistryRegulatoryDetectionHumanTranscription factorsCellAssaysMouseFragmentsOptimal
Immunogen3
- Hamster protein was used as the immunogen for this Cyclin B1 antibody. (nsjbio.com)
- A portion of amino acids 60-110 of human ID2 was used as the immunogen for this antibody. (novusbio.com)
- The polyclonal antiserum reacts with human and mouse cyclin C. A polypeptide fragment containing amino acids 290-303 (QGPNGSQNSSYSQS) at the C-terminus of human cyclin C, with the addition of a C-terminal cysteine (C) residue to facilitate conjugation to KLH, was used as immunogen. (bdbiosciences.com)
Protein28
- Quality control test: Antibody Reactive Against Recombinant Protein. (novusbio.com)
- Antibody reactivity against cell lysate and recombinant protein for WB. (novusbio.com)
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (novusbio.com)
- This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. (novusbio.com)
- This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. (novusbio.com)
- Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene. (rockland.com)
- Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue. (rockland.com)
- Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. (enquirebio.com)
- This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. (nsjbio.com)
- The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. (nsjbio.com)
- Cyclin-dependent kinase 5 is a protein, and more specifically an enzyme, that is encoded by the Cdk5 gene. (wikipedia.org)
- Orthologous to human CDK2AP1 (cyclin dependent kinase 2 associated protein 1). (nih.gov)
- Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. (nih.gov)
- The identity of this protein has not been determined, but may represent an alternative splice product of the cyclin C gene, as has been identified in other species. (bdbiosciences.com)
- Because Cdc2 kinase is important for cell entry into mitosis, cyclin C's ability to regulate cell cycle progression may be attributed, in part, to modulation of Cdc2 protein expression.6 Cyclin C has a predicted molecular weight of 36 kD. (bdbiosciences.com)
- The two antibodies function as a capture-target-detection sandwich (#46835 and #79774, respectively) to detect endogenous protein expression in positive and negative controls (with or without phosphatase inhibitors). (cellsignal.com)
- This antibody detects endogenous level of total CDC2 protein. (prosci-inc.com)
- Western blotting was performed to detect the protein levels of cyclin‑dependent kinase inhibitor P21 (P21), B‑cell lymphoma‑2 (Bcl‑2), matrix metalloproteinase 9 (MMP9) and E26 oncogene homolog 1 (ETS1). (spandidos-publications.com)
- Mice that were vaccinated to boost their immune response against this cell cycle protein, called cyclin B1, were able to reject a tumor challenge in which they were exposed to a cancer cell line that overproduced it, explained senior author Olivera Finn, Ph.D., Distinguished Professor and chair of the Department of Immunology at the Pitt School of Medicine. (scienceblog.com)
- While we were studying it, we noted that many healthy people already had an immune response, or antibodies, against the protein, even though they'd never had cancer. (scienceblog.com)
- Because cyclin B1 is a 'self' protein, there have been concerns that boosting the immune response against it would produce autoimmunity and create new problems," Dr. Finn said. (scienceblog.com)
- This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). (neobiotechnologies.com)
- Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. (neobiotechnologies.com)
- We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
- Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)
- Epstein-Barr virus latent membrane protein expression and cyclin DI cell cyase protein expression in malignant and normal oesophageal tissues to see whether any variation in their expression in these tissues could be of diagnostic or prognostic value. (bvsalud.org)
- EBV-LMPI protein expression and cyclin DI expression were studied immunohisto chemically in these tissue sections. (bvsalud.org)
- The various markers that enable assessment of the progression of preneoplastic lesions to spindle cell carcinoma include the p16 protein, which halts the cell cycle and induces apoptosis by pRb-mediated phosphorylation of cyclin-dependent kinase 4 (CDK4). (bvsalud.org)
Polyclonal6
- Our Cyclin E1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. (phosphosolutions.com)
- The antibody is a rabbit polyclonal antibody raised against CCND1. (uscnk.com)
- The polyclonal antibodies (Cat. (bdbiosciences.com)
- The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. (bdbiosciences.com)
- The following product was used in this experiment: Phospho-MCM2 (Ser41) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-114602, RRID AB_2899238. (thermofisher.com)
- Description: A polyclonal antibody against PPP1CA. (pp1a.com)
Isotype1
- Red=isotype control, Blue= Cyclin B1 antibody. (nsjbio.com)
Kinases6
- Cyclins function as regulators of CDK kinases. (novusbio.com)
- Scientific Background G1/S-specific cyclin-D1 (CCND1) is a member of the Cyclin family which act as regulators of CDK kinases. (biosensis.com)
- Even though Cdk5 has a similar structure to other cyclin-dependent kinases, its activators are highly specific (CDK5R1 and CDK5R2). (wikipedia.org)
- Unlike other cyclin-dependent kinases, CDK5 does not also require phosphorylation on the T loop. (wikipedia.org)
- Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
- As a biologically important example we have studied the complex formed by cyclins and cyclin-dependent kinases (CDKs), which play an essential role in the control of the eukaryotic cell cycle. (lu.se)
Reactivity2
- Cross reactivity with Cyclin B1 from other species may also occur. (rockland.com)
- Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user. (rockland.com)
Proteins3
- Abcam: antibodies, proteins, kits. (abcam.com)
- Cyclin B1 and MUC1 are part of a big family of self-proteins that become over-produced during cancer development, so they have great potential as targets in prevention vaccines. (scienceblog.com)
- These methods are powerful and effective for identifying antibodies that bind to targets, yet they depend on the secretory pathway to transport proteins that will be displayed 14-16 . (jove.com)
Antigen10
- It detects human Cyclin E1 and is antigen affinity purified. (phosphosolutions.com)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. (biosensis.com)
- Reference: Immunocytochemical identification of proliferative hepatocytes using monoclonal antibody to proliferating cell nuclear antigen (PCNA/cyclin). (neb.com)
- The authors investigated whether immunocytochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA/cyclin) could be used to identify proliferative hepatocytes in frozen sections fixed in a mixture of periodate, lysine, and 2% paraformaldehyde. (neb.com)
- Antibodies engineered for intracellular function must not only have affinity for their target antigen, but must also be soluble and correctly folded in the cytoplasm. (jove.com)
- Here, we describe a protocol to screen a recombinant library of single-chain variable fragment (scFv) antibodies for antigen-binding and proper cytoplasmic folding simultaneously. (jove.com)
- Antigen-binding and cytoplasmic solubility can be improved with subsequent rounds of mutagenesis and screening to engineer antibodies with high affinity and high cytoplasmic solubility for intracellular applications. (jove.com)
- Although antibodies have much potential for intracellular applications, engineering them for proper folding and solubility in the intracellular environment while maintaining the ability to bind to a target antigen is challenging. (jove.com)
- HHV-8 latency-associated nuclear antigen-1 and HHV-8 viral cyclin gene were identified in the lung tissue of 10 (62.5%) of 16 patients with IPAH, whereas only 1 (7.1%) of 14 patients with associated pulmonary hypertension (PH) had HHV-8 gene sequences in lung tissue ( 2 ). (cdc.gov)
- Mib-1 monoclonal antibody recognizes the proliferation-related antigen Ki-67. (lu.se)
Datasheet1
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
Phosphorylation5
- In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. (nsjbio.com)
- and (v) pRb phosphorylation and cyclin D1 expression are reduced in K10-transfected cells and increased in K16-transfected cells. (exposed-skin-care.net)
- The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. (neobiotechnologies.com)
- Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. (neobiotechnologies.com)
- DARPP-32 can itself inhibit the following PKA DARPP-32 phosphorylation by cyclin-dependent kinase 5 (Cdk5). (pp1a.com)
Specificity1
- Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity. (rockland.com)
Monoclonal antibodies3
- Monoclonal Antibodies Use Chicken Eggs? (biolisp.org)
- Chicken Antibody Laboratories manufactures the monoclonal antibodies use chicken eggs? (biolisp.org)
- Dynamics of RAS/BRAF Mutations in cfDNA from Metastatic Colorectal Carcinoma Patients Treated with Polychemotherapy and Anti-EGFR Monoclonal Antibodies. (cdc.gov)
Western Blot6
- Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - CCNH monoclonal antibody (M01), clone 1B8 Analysis of CCNH expression in HeLa. (novusbio.com)
- Western Blot of transfected 293T cells showing specific immunolabeling of Cyclin E1. (phosphosolutions.com)
- Western Blot: ID2 Antibody [NBP2-27194] - Analysis using ID2 antibody. (novusbio.com)
- Western blot analysis of cyclin C in NIH 3T3 mouse fibroblasts. (bdbiosciences.com)
- The antibodies may be used for western blot analysis (1:1000) and immunoprecipitation (1:100). (bdbiosciences.com)
- Western blot Kit for Chicken Primary Antibodies, Chemilum. (biolisp.org)
Lysate3
- WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
- Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. (rockland.com)
- Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate. (sigmaaldrich.com)
Kinase inhibitor1
- It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. (origene.com)
ELISA2
- Sandwich ELISA: Cyclin H Antibody (1B8) [H00000902-M01] - Detection limit for recombinant GST tagged CCNH is approximately 1ng/ml as a capture antibody. (novusbio.com)
- The Phospho-GSK-3β (Ser9) Matched Antibody Pair is ideal for use with immunoassay technologies and high-throughput ELISA platforms requiring antibody pairs with specialized or custom antibody labeling. (cellsignal.com)
Secondary Antibody1
- Conjugation service and the corresponding secondary antibody are available. (biomatik.com)
Custom antibody services1
- Founded in 2001, we are committed to offering the highest quality antibodies, custom antibody services, and customer support in the business. (phosphosolutions.com)
CDC24
- Cyclin C associates with Cdk8, forming a complex which can induce gene transcription of Cdc2 (Cdk1). (bdbiosciences.com)
- CDC2 (Ab-161) antibody was raised against a peptide sequence around aa.159~163 (T-Y-T-H-E) derived from Human CDC2. (prosci-inc.com)
- Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. (caslab.com)
- The antibody is specific to the 2 isoforms of CDC2. (caslab.com)
CCND13
- Magnetic Luminex Assay Kit for Cyclin D1 (CCND1) ,etc. (uscnk.com)
- Cyclin D1 is also known as BCL1, B Cell Lymphoma #1, CCND1. (enquirebio.com)
- Description: A Mouse monoclonal antibody against Chicken Cyclin D1 (CCND1). (biolisp.org)
HeLa2
- Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. (novusbio.com)
- Immunofluorescent staining of PFA-fixed human HeLa cells with Cyclin B1 antibody (clone V92.1). (nsjbio.com)
Clone1
- SDS-PAGE analysis of purified, BSA-free Cyclin B1 antibody (clone V92.1) as confirmation of integrity and purity. (nsjbio.com)
Cdks1
- Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate progression of the cell cycle. (bdbiosciences.com)
Species1
- Cyclin C has been identified in both human and chicken species. (bdbiosciences.com)
Gene3
- also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. (sigmaaldrich.com)
- The identity of the higher molecular weight band (~ 45 kD) has not been determined, but may represent an alternative splice product of the cyclin C gene. (bdbiosciences.com)
- Cyclin C may play a dual role within the cell in its ability to regulate both cell cycle progression as well as gene transcription. (bdbiosciences.com)
Azide3
- Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide). (nsjbio.com)
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. (prosci-inc.com)
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. (neobiotechnologies.com)
Overexpression2
Concentration2
- Antibody concentration 10 ug/ml. (novusbio.com)
- Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. (biosensis.com)
Tumor3
- Cyclin E1 expression is elevated in human tumor cells. (phosphosolutions.com)
- In hepatocellular carcinoma, many PCNA/cyclin-positive tumor cells were seen throughout the neoplasms. (neb.com)
- Cells infected with chicken pox virus, for example, look very much like tumor cells because they, too, overproduce cyclin B1. (scienceblog.com)
Lymphoma1
- Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression. (spandidos-publications.com)
Substrate1
- Hepatocytes that were positive for PCNA/cyclin were observed in both types of substrate specimens. (neb.com)
Subunits1
- Dysregulation of CDK8 (Cyclin-Dependent Kinase 8) and its regulatory partner CycC (Cyclin C) , two subunits of the conserved Mediator (MED) complex, have been linked to diverse human diseases such as cancer. (sdbonline.org)
Fitc1
- This antibody is labeled with FITC. (biolisp.org)
Affinity1
- Antibodies were purified by affinity-chromatography using epitope-specific peptide. (prosci-inc.com)
Dependent3
- The molecule belongs to the cyclin-dependent kinase family. (wikipedia.org)
- The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. (sigmaaldrich.com)
- The Potential for Selective Cyclin-Dependent Kinase 4/6 Inhibition in the Therapy for Head and Neck Squamous Cell Carcinoma. (cdc.gov)
Peptide1
- A synthetic peptide from C-terminus of human cyclin D1. (enquirebio.com)
Mitotic2
- Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (novusbio.com)
- This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. (nsjbio.com)
Immunohistochemistry2
- Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. (novusbio.com)
- Immunohistochemistry analysis in human placenta and kidney tissues using AMAb91497 antibody. (atlasantibodies.com)
Regulatory1
- This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)
Detection1
- Matched Antibody Pairs include capture and detection antibodies to non-overlapping epitopes. (cellsignal.com)
Human4
- IHC-P analysis of human tonsil tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
- Human G1/S-specific cyclin-D1 has a predicted length of 295 residues and MW of 34 kDa. (biosensis.com)
- Human Cyclin C polypeptide fragment aa. (bdbiosciences.com)
- Human herpesvirus 8 (HHV-8) antibodies were detected in 1 of 33 patients with pulmonary hypertension (including in 1 of 16 with idiopathic pulmonary arterial hypertension), 5 of 29 with cystic fibrosis, and 3 of 13 with interstitial lung disease. (cdc.gov)
Transcription factors1
- Alterations in expression include metabolic genes (CYP11B1, MGMT), cell cycle control and DNA repair genes (GADD45, Cyclin G2, Cyclin D3, Cyclin A) and transcription factors (IF2, IF3). (cdc.gov)
Cell4
- It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
- More recently, cyclins are being shown to have additional functions not restricted to cell cycle regulation. (bdbiosciences.com)
- Group 3 cyclins (G1, G2 and I) may play a role distinct from either cell cycle or transcriptional regulation. (bdbiosciences.com)
- Like many cyclins, the expression of cyclin C oscillates throughout the cell cycle, with a peak observed during G1 phase. (bdbiosciences.com)
Assays2
- To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)
- We performed assays for antibodies directed to lytic antigens of HHV-8 in plasma samples, according to a previously well-described method ( 7 ). (cdc.gov)
Mouse5
- Product Description google Mouse anti-G1/S-specific cyclin D1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded. (biosensis.com)
- No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting. (sigmaaldrich.com)
- Description: A Mouse monoclonal antibody against Chicken Interleukin 2 (IL2). (biolisp.org)
- Mouse monoclonal antibody to BiP/GRP78. (exposed-skin-care.net)
- SDS-PAGE Analysis Purified Cyclin B2 Mouse Monoclonal Antibody (X29.2). (neobiotechnologies.com)
Fragments1
- The reducing cytoplasmic environment prevents the formation of the disulfide bonds normally required for the stable folding of full-length antibodies and antibody fragments, including single-chain variable fragment (scFv) antibodies 6,7 . (jove.com)
Optimal1
- Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. (nsjbio.com)