• Synthesis and evaluation of pyrazolo[1,5-b]pyridazines as selective cyclin dependent kinase inhibitors. (expasy.org)
  • Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. (wuxibiortus.com)
  • The 96 kDa protein kinase activated by oncogenic Ras in Xenopus egg extracts is also activated by constitutively active Mek: activation requires serine/threonine phosphorylation. (wikigenes.org)
  • 2018) Discovery of 3-Benzyl-1-( trans-4-((5-cyanopyridin-2-yl)amino)cyclohexyl)-1-arylurea Derivatives as Novel and Selective Cyclin-Dependent Kinase 12 (CDK12) Inhibitors. (guidetopharmacology.org)
  • protein kinase, DNA-act. (gsea-msigdb.org)
  • Untimely cyclin A2-associated kinase activity in meiosis I leads to precocious sister separation, whereas inhibition of cyclin A2 in meiosis II prevents it. (ox.ac.uk)
  • Treatment with the stress agent prostaglandin A 2 (PGA 2 ) induces expression of the cyclin-dependent kinase inhibitor p21. (johnshopkins.edu)
  • Here, we present evidence that p21 expression increases through PGA 2 -triggered stabilization of the p21 mRNA and further show that these events require the mitogen-activated protein (MAP) kinase ERK. (johnshopkins.edu)
  • AMP-activated protein kinase (AMPK) regulates biological functions in tumors that are mediated by liver kinase B1 (LKB1), such as cell survival and transcription, via the mTOR pathway [33]. (geogise.com)
  • The structures of pCDK2/cyclin A/variolin B and pCDK2/cyclin A/meriolin 3 complexes reveal that the two inhibitors bind within the ATP binding site of the kinase, but in different orientations. (rcsb.org)
  • The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. (cancerindex.org)
  • Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) interacts with CDK2AP2, modulates the actions of transforming growth factor-B1, cyclin-dependent kinase 2 and retinoblastoma protein, and closely interacts with micro-RNA21 and micro-RNA25. (cancerindex.org)
  • Assay of the mutants with a cyclin-dependent kinase 4-selective bisanilinopyrimidine shows that the K89T mutation is primarily responsible for the selectivity of this compound. (rcsb.org)
  • Use of the cyclin-dependent kinase 2-selective 6-cyclohexylmethoxy-2-(4'-sulfamoylanilino)purine (NU6102) shows that K89T has no role in the selectivity, while the remaining three mutations have a cumulative influence. (rcsb.org)
  • In addition, phosphorylated ERK1/2 and p38, key members of mitogen-activated protein kinase (MAPK) family regulating BCa tumorigenesis, were strongly decreased. (spandidos-publications.com)
  • Recent studies have shown that the cyclin A2-CDK1 complex triggers cyclin B1-CDK1 activation which results in chromatin condensation and the breakdown of the nuclear envelope. (wikipedia.org)
  • controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. (wuxibiortus.com)
  • The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle. (wuxibiortus.com)
  • A ) Quantitative PCR analysis of CDK1, cyclin A2, CDK2, cyclin E2 and CYP1B1 mRNA levels in macrophages treated with carrier (DMSO), AhR agonist or AhR antagonist. (elifesciences.org)
  • B ) Western blot analysis of HSP90 (loading control) CDK1, cyclin A2, CDK2, cyclin E2 protein levels in macrophages treated with carrier (DMSO), AhR agonist or antagonist. (elifesciences.org)
  • A ) Levels of SAMHD1, phosphorylated SAMHD1, CDK1 and CDK2 proteins in activated or unsimulated, unfractionated PBMCs treated with carrier, AhR agonist or antagonist. (elifesciences.org)
  • B ) Levels of SAMHD1, phosphorylated SAMHD1, CDK1 and CDK2 proteins in activated or unstimulated purified CD4+ T cells treated with carrier, AhR agonist or antagonist. (elifesciences.org)
  • The model was built by integrating and modifying two previously published models such that the model outputs for cyclins A and B fit cyclin expression measurements and the activation of B cyclin/Cdk1 coincided with phosphorylation of histone H3. (ebi.ac.uk)
  • The model depends on Cdh1-regulated cyclin degradation during G1, regulation of B cyclin/Cdk1 activity by cyclin A/Cdk via Wee1, and transcriptional control of the mitotic cyclins that reflects some of the current literature. (ebi.ac.uk)
  • Phosphorylation at CDK1, CDK4, and CDK9 sites on, respectively, protein phosphatase 1alpha, retinoblastoma protein, and RNA polymerase II is inhibited in neuroblastoma SH-SY5Y cells exposed to meriolins. (rcsb.org)
  • Cyclin A2 mediates the abundance of MRE11 nuclease by binding to Mre11 mRNA. (wikipedia.org)
  • Also in mice, cyclin A2 was found to be an RNA binding protein that controls the translation of Mre11 mRNA. (wikipedia.org)
  • Binding experiments using either endogenous p21 mRNA or in vitro-labeled p21 transcripts revealed a specific PGA 2 -dependent association of the p21 mRNA with the RNA-binding protein HuR. (johnshopkins.edu)
  • protein_coding" "Cz03g40300.t1","No alias","Chromochloris zofingiensis","Pre-mRNA processing factor 4 (PRP4)-like [Interproscan]. (ntu.edu.sg)
  • protein_coding" "Cz05g30030.t1","No alias","Chromochloris zofingiensis","mRNA splicing factor Cwf21 domain [Interproscan]. (ntu.edu.sg)
  • In human BCa tissues, we observed that LAPTM5 was significantly induced at both mRNA and protein levels, which is consistent with our microarray result. (spandidos-publications.com)
  • Cyclin-A2 is a protein that in humans is encoded by the CCNA2 gene. (wikipedia.org)
  • Cyclin A2 (Ccna2) is a key protein involved in the direction of mammalian cardiac myocytes to grow and divide, and has been shown to induce cardiac repair following myocardial infarction. (wikipedia.org)
  • Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated in the initiation and progression of DNA synthesis. (wikipedia.org)
  • The cyclin A2-CDK2 complex eventually phosphorylates E2F, turning off cyclin A2 transcription. (wikipedia.org)
  • CDK2/Cyclin A2-H00A-Wuxi Biortus Biosciences Co. Ltd. (wuxibiortus.com)
  • Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. (wuxibiortus.com)
  • We have previously demonstrated that Cyclin A2 is involved in cytoskeletal dynamics, epithelial-mesenchymal transition (EMT) and metastasis. (cnrs.fr)
  • Furthermore, proteins involved in epithelial-mesenchymal transition (EMT) were strongly altered, which plays a central role in metastasis. (spandidos-publications.com)
  • Evidence suggests that both cell cycle inhibitors (such as P21 and P27) and promoters (such as cyclins E1 and D1) play a vital role in malignancy progression [37C39]. (geogise.com)
  • It is one of the two types of cyclin A: cyclin A1 is expressed during meiosis and embryogenesis while cyclin A2 is expressed in dividing somatic cells. (wikipedia.org)
  • Cyclin A2 is required for sister chromatid segregation, but not separase control, in mouse oocyte meiosis. (ox.ac.uk)
  • Accordingly, endogenous cyclin A is localized to kinetochores throughout meiosis II, but not in anaphase I. Additionally, we found that cyclin B1, but not cyclin A2, inhibits separase in meiosis I. These findings indicate that separase-dependent cohesin removal is differentially regulated by cyclin B1 and A2 in mammalian meiosis. (ox.ac.uk)
  • We have demonstrated that cyclin A1 is essential for the progression of spermatocytes into the first meiotic division, that cyclin A2 is required for mitotic divisions of male germ cell stem cells, and that the E-type cyclins have unexpected function during meiosis, notably in the maintenance of telomere integrity. (columbia.edu)
  • Absence of cyclin A2 before the R point is due to the E2F inhibition by hypophosphorylated retinoblastoma protein (pRb). (wikipedia.org)
  • Humans have evolved elaborate mechanisms to activate p53 in response to insults that lead to cancer, including the binding and inhibition of Hdm2 by the 60S ribosomal proteins (RPs) RPL5 and RPL11. (nih.gov)
  • Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
  • E2F promotes cyclin A2 transcription by de-repressing the promoter. (wikipedia.org)
  • During mouse development and aging, cyclin A2 promotes DNA repair, particularly double-strand break repair, in the brain. (wikipedia.org)
  • Lasp1 (LIM and SH3 domain protein 1) promotes tumor proliferation and invasion in multiple cancer entities including non-small cell lung cancer (NSCLC). (oncotarget.com)
  • and Specifically, by activating the transforming growth-factor (TGF-) pathway, deletion of HDAC10 promotes the expression of sex-determining region Y box protein 9 CP-640186 (SOX9), which subsequently up-regulates the expression of SLUG, as well as CD44. (geogise.com)
  • Wu M, Sun Y, Xu F, Liang Y, Liu H, Yi Y. Annexin A2 Silencing Inhibits Proliferation and Epithelial-to-mesenchymal Transition through p53-Dependent Pathway in NSCLCs. (jcancer.org)
  • Cyclin A2 transcription is mostly regulated by the transcription factor E2F and begins in G1, after the R point. (wikipedia.org)
  • After the R point, pRb is phosphorylated and can no longer bind E2F, leading to cyclin A2 transcription. (wikipedia.org)
  • We introduced autocatalytic transcription of E2F, E2F regulated transcription of cyclin B, Cdc20/Cdh1 mediated E2F degradation, enhanced transcription of mitotic cyclins during late S/early G2 phase, and the sustained synthesis of cyclin B during mitosis. (ebi.ac.uk)
  • After fusion, retroviruses release genomes and associated proteins into the cytoplasm during the uncoating process, ultimately resulting in the synthesis of reverse transcription products, formation of the preintegration complex, and nuclear import [ 7 ]. (biomedcentral.com)
  • Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs. (wikigenes.org)
  • We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
  • Previous studies have shown that by inhibiting histone H3 deacetylation round the let-7f-2/miR-98 promoter, HDAC10 suppresses HMGA2 expression which target to CP-640186 cyclin A2 promoter, and further inhibits the transcription of cyclin A2 [30,36]. (geogise.com)
  • The first of the three major projects involves understanding the function of the A and E-type cyclins during the mitotic and meiotic cell cycles mainly during spermatogenesis but also in oogenesis. (columbia.edu)
  • Our findings will broaden our knowledge on how Cyclin A2 contributes to EMT and metastasis. (cnrs.fr)
  • As a result, Annexin A2 normally contributes to clearance of fibrin formation on the blood vessel surface in the case of minor vascular injury [ 8 ]. (jcancer.org)
  • This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
  • The SH3 domain is a 60 amino acids segment shared by diverse structural and signaling proteins [ 6 ]. (oncotarget.com)
  • Cyclin A2 regulates homologous recombinational DNA repair in human breast cancer cells Loss of cyclin A2 causes high rates of double-strand breaks in DNA. (wikipedia.org)
  • Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). (wuxibiortus.com)
  • Annexin A2 has been involved in cancer cell adhesion, invasion and metastasis. (jcancer.org)
  • Clinicopathologic analysis showed that Annexin A2 expression was significantly correlated with clinical stage, and lymph node metastasis. (jcancer.org)
  • Extensive researches demonstrate that Annexin A2 plays important roles in tumor proliferation, invasion and metastasis [ 12 - 14 ]. (jcancer.org)
  • Cyclin A2 mediates the abundance of RAD51 protein by inhibiting proteasome degradation of this protein. (wikipedia.org)
  • The mechanism, which is based on the synthesis and degradation of three ''master regulator'' proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. (ebi.ac.uk)
  • This observation suggests the existence of one or more proteins induced or activated by IFNα that directly or indirectly protect SAMHD1 from degradation. (biomedcentral.com)
  • In Cyclin A2-depleted normal murine mammary gland (NMuMG) cells expressing RasV12, we found that beta-catenin was liberated from the cell membrane and cell-cell junctions and underwent nuclear translocation and activation. (cnrs.fr)
  • Here, we dissected the pathways that are responsible for EMT in cells deficient for Cyclin A2. (cnrs.fr)
  • activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. (wuxibiortus.com)
  • It reproduces protein time courses in wild-type cells, mimics correctly the phenotypes of many mutant strains, and predicts the phenotypes of currently uncharacterized mutants. (ebi.ac.uk)
  • Annexin A2 (also called ANXA2) is mainly expressed in endothelial cells. (jcancer.org)
  • More importantly, we found that IPMK interacted with AMPK and functioned as a positive regulator of AMPK protein in osteosarcoma cells. (ijbs.com)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • The mission of the Pittsburgh Center for HIV Protein Interactions (PCHPI) is to understand HIV maturation and the early cellular events of HIV infection by characterizing HIV-host cell protein interactions/complexes at a high-resolution, molecular level. (pitt.edu)
  • After translation, where is a protein localised, and what complexes should it join? (biomedcentral.com)
  • 5. Strazic Geljic I, Kucan Brlic P, Musak L, Karner D, Ambriović‐Ristov A, Jonjic S, Schu P, Lenac Rovis T: Viral Interactions with Adaptor‐Protein Complexes: A Ubiquitous Trait among Viral Species. (com.hr)
  • As loss of RPL5/RPL11 abrogates ribosome biogenesis and protein synthesis to the same extent as loss of other essential 60S RPs, we reasoned the loss of RPL5 and RPL11 would induce a p53-independent cell cycle checkpoint. (nih.gov)
  • Next, protein-protein interaction (PPI) networks were used to further explore the biomarkers with the most interactions in gene modules. (biomedcentral.com)
  • What does this gene/protein do? (cancerindex.org)
  • GSE76211) ( 3 , 4 ), revealing a significantly upregulated gene in BCa tissues, the lysosomal-associated protein multispanning transmembrane 5 (LAPTM5). (spandidos-publications.com)
  • The signaling pathways: HDAC10-let-7f-2/miR-98-HMGA2-cyclin A2 arrests the G2/M transition and finally inhibits lung malignancy cell proliferation. (geogise.com)
  • Current projects are focused on CA and capsid interactions, proteins involved in trafficking and nuclear import, retroviral intasome structure, and analyses of the HIV-1 pre-integration complex. (pitt.edu)
  • A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
  • In addition, a PPI network can be modeled as an undirected graph, where vertices represent proteins and edges represent interactions between proteins, to prioritize disease associated genes or pathways and to understand the modus operandi of disease mechanisms [ 9 , 10 ]. (hindawi.com)
  • Many computational approaches have been proposed to assess the reliability of protein interactions data. (hindawi.com)
  • The InterFeron Induced TransMembrane (IFITM) proteins are interferon stimulated genes that restrict many viruses, including HIV-1. (biomedcentral.com)
  • The objective of this work is to identify dysregulated genes and pathways of ccRCC temporally according to systematic tracking of the dysregulated modules of reweighted Protein-Protein Interaction (PPI) networks. (hindawi.com)
  • The Human Genome Project has revealed that protein-coding genes represent less than 2% of the total genome sequence [ 7 ], and the remaining greater portion of human genomes are regarded as "junk DNAs", for they do not encode any protein. (oncotarget.com)
  • The levels of cyclin A2 are tightly synchronized with the progression of the cell cycle. (wikipedia.org)
  • We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
  • However, the exact function and mechanism of Annexin A2 in tumor progression of NSCLCs have not been elucidated. (jcancer.org)
  • Taken together, these data suggest that Annexin A2 plays an important role in NSCLCs progression, which could serve as a potential prognosis marker and a novel therapeutic target for NSCLCs. (jcancer.org)
  • However, the exact function and mechanism of Annexin A2 in tumor progression of NSCLCs need to be further determined. (jcancer.org)
  • Components of the canonical wingless (WNT) pathway, including WNT8b, WNT10a, WNT10b, frizzled 1 and 2 and TCF4 were upregulated at the messenger RNA and protein levels following Cyclin A2 depletion. (cnrs.fr)
  • The increase in double-strand breaks is a consequence of defective homologous recombinational repair of the breaks resulting from reduced MRE11 and RAD51 DNA repair proteins. (wikipedia.org)
  • Many of these ISGs encode proteins that have antiviral activity, inhibiting viruses at various steps of their life cycle including at the earliest stages of entry [ 1 ]. (biomedcentral.com)
  • Although lncRNAs cannot encode any functional protein, they are involved in diverse biological processes, playing essential roles in maintaining cell growth, differentiation and proliferation [ 13 - 15 ]. (oncotarget.com)
  • Incorporation of both FAK inhibitor and AKT inhibitor counteracted the upregulating expression of Cyclin A2, CyclinB1, and Snail, and downregulating expression of E-cadherin expression induced by Lasp1 overexpression. (oncotarget.com)
  • A second area of research involves elucidating the function of the BET family of double bromodomain-containing proteins, proteins that read epigenetic marks, during germ cell differentiation and neural development. (columbia.edu)
  • While it is not clear whether increased expression of cyclin A2 is a cause or result of tumorigenesis, it is indicative of prognostic values such as predictions of survival or relapse. (wikipedia.org)
  • Reduced CDK/cyclin, phospho-SAMHD1 and dTNP levels in macrophages following activation of AhR. (elifesciences.org)
  • Some lentiviruses evolved Vpx/Vpr proteins to degrade SAMHD1. (biomedcentral.com)
  • We found that IFITM proteins contribute to the IFNα-mediated protection of SAMHD1 by blocking VSV-G-mediated entry of the lentiviral particles delivering Vpx. (biomedcentral.com)
  • The recognition of SAMHD1 by Vpx/Vpr proteins is evolutionarily dynamic, with some viral proteins binding to the N-terminus of SAMHD1, and others to the C-terminus [ 17 ]. (biomedcentral.com)
  • Tumour-derived PTH-related protein triggers adipose tissue browning and cancer cachexia. (phoenixpeptide.com)
  • Parathyroid hormone-related protein (PTHrP) is a critical regulator of bone resorption and augments osteolysis in skeletal malignancies. (phoenixpeptide.com)
  • Cyclin A2 is involved in the G2/M transition but it cannot independently form a maturation promoting factor (MPF). (wikipedia.org)
  • Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. (wuxibiortus.com)
  • Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)
  • A widely-expressed cyclin A subtype that functions during the G1/S and G2/M transitions of the CELL CYCLE. (uams.edu)
  • Cell cycle ratio and apoptosis in the two groups were determined using flow cytometry, while Pi3k/Akt signal route-linked protein levels were assayed by immunoblot method. (ajol.info)
  • Since many of the proteins involved in regulating the cell cycle of C. crescentus are conserved among many genera of a-proteobacteria, the proposed mechanism may be applicable to other species of importance in agriculture and medicine. (ebi.ac.uk)
  • In this study, we showed that Annexin A2 was evidently overexpressed in human NSCLCs cell lines and NSCLCs tissues. (jcancer.org)
  • Numbers of proteins and signaling are involved in cell apoptosis. (geogise.com)
  • discovered that a low degree of HDAC10 in gastric tumor might activate proapoptotic substances including caspase-3, caspase-9, and Bet through the thioredoxin interacting proteins (TXNIP)-induced ROS signaling pathway [46] (Body 1B). (geogise.com)
  • Lasp1 (LIM and SH3 domain protein 1), localized on chromosome 17q11-21.3, was first identified in 1995 [ 1 ]. (oncotarget.com)
  • Increased expression of cyclin A2 has been observed in many types of cancer such as breast, cervical, liver, and lung among others. (wikipedia.org)
  • Subsequent western blot results revealed that Lasp1 promoted the expression of Cyclin A2, CyclinB1, and Snail, and inhibited the expression of E-cadherin. (oncotarget.com)
  • Sequential expression of multiple POU proteins during amphibian early development. (wikigenes.org)
  • Kaplan-Meier analysis revealed that patients with high Annexin A2 expression had poorer overall survival rates than those with low Annexin A2 expression. (jcancer.org)
  • HDAC10 plays an oncogenic role by inhibiting the expression of P27, P21 and enhancing that of cyclins D1 and E1 [29] (Physique 1A). (geogise.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • We recently identified a unique peptide fragment of parathyroid hormone-related protein (PTHrP), PTHrP(12-48), as a validated serum biomarker in breast cancer patients that correlates with and predicts the presence of bone metastases. (phoenixpeptide.com)
  • It has been established that overexpressed anti-apoptotic proteins (such as those in the Bcl-2 family) as well as down-regulated proteins (such as for example Bet, BAK) and BIK may disrupt the total amount between apoptosis and anti-apoptosis [41,42]. (geogise.com)