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  • 1997
  • In addition, we exposed the tissue to a number of pharmacological agents that activate or inhibit the putative intracellular pathway at different points (see Fig. 1 ), starting with NMDA receptors and including protein kinase G (PKG), a likely target of cGMP ( Wang and Robinson, 1997 ). (jneurosci.org)
  • heat-shock pr
  • A family of heat-shock proteins that contain a 70 amino-acid consensus sequence known as the J domain. (bioportfolio.com)
  • The J domain of HSP40 heat shock proteins interacts with HSP70 HEAT-SHOCK PROTEINS. (bioportfolio.com)
  • HSP40 heat-shock proteins play a role in regulating the ADENOSINE TRIPHOSPHATASES activity of HSP70 heat-shock proteins. (bioportfolio.com)
  • A subfamily of small heat-shock proteins that are closely related to ALPHA B-CRYSTALLIN. (bioportfolio.com)
  • Heat shock proteins are induced by activation of heat shock factor 1 (HSF1) in response to heat shock and protect against heat stress. (bioportfolio.com)
  • Thermotolerance is a phenomenon in which cells become resistant to stress by prior exposure to heat shock, and its development is associated with the induction of heat shock proteins (Hsps), including. (bioportfolio.com)
  • They play essential roles as transcriptional activators of the HEAT-SHOCK RESPONSE by inducing expression of large classes of MOLECULAR CHAPERONES and heat-shock proteins. (bioportfolio.com)
  • residues
  • Although it is generally accepted that many NO effects are mediated via elevation of intracellular cGMP, one should keep in mind that NO can exert effects independent of cGMP production, for instance via modification of cellular proteins by S-nitrosylation of cysteine residues. (ahajournals.org)
  • cellular proteins
  • these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as putative interacting proteins using mass spectrometry. (embl-heidelberg.de)
  • We have found that a combination of: 1) the variable sequence of the C-terminus of the bait with 2) a C-terminal valine "cloning scar" present in a commercially available ORF library, can in some cases create a peptide motif that results in the aberrant co-purification of endogenous cellular proteins. (embl-heidelberg.de)
  • ligands
  • These proteins are localized to the actin cytoskeleton and are in vitro and in vivo ligands for the focal adhesion-associated proteins zyxin and vinculin and the actin-binding protein profilin ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ). (jimmunol.org)
  • hippocampal
  • Full length wt PKG II and wt regulatory PKG JJ proteins, when overexpressed in hippocampal neurons targeted to the synapse, however, a G2A PKG II mutant, which does not undergo myristoylation had a much more diffuse distribution in the entire cell and did not target to the synapse. (ubc.ca)
  • inhibit
  • Does cGMP, via protein kinase G, inhibit cAMP-stimulated Ca 2+ current (I Ca(L) ) in mammalian ventricular myocytes by phosphorylating the calcium channel at a site different from that acted on by cAMP or by dephosphorylating the calcium channel through phosphatase(s)? (aspetjournals.org)
  • EGFR
  • Sulindac downregulates both pEGFR and total EGFR and pERK by 12 hours in a dose dependent manner without affecting total ERK levels and it induces apoptosis by 24 hours. (ucdenver.edu)
  • muscarinic
  • The negative inotropism was insensitive to losartan and CGP42112 (AT 1 and AT 2 ANG II receptor antagonists, respectively), and was abrogated by the AT 1 receptor antagonist CV11974, the G protein blocker pertussis toxin (PTx) and the muscarinic antagonist atropine. (biologists.org)
  • presynaptic
  • We then determined whether cGMP is present in presynaptic retinal terminals by the use of double-labeling for cGMP and either the presynaptic protein synaptophysin or a fluorescent anterograde tracer injected into the eye to label retinal terminals unambiguously in the dLGN. (jneurosci.org)
  • In contrast, blockers of the ADP-ribosyltransferase primarily reduced LTP, suggesting that NO, at least partly, acts via ADP-ribosylation of presynaptic proteins. (jneurosci.org)
  • targets
  • Additionally, mutations within pseudokinase domains are known to underlie human diseases, suggesting that these proteins may represent new and unexplored therapeutic targets. (embl-heidelberg.de)
  • soluble
  • In this report, the water-soluble periplasmic sensor domain of Sinorhizobium meliloti DctB (DctBp) was studied, and three crystal structures were solved: the apo protein, a complex with C(4) succinate, and a complex with C(3) malonate. (embl-heidelberg.de)
  • Expression
  • The expression of paxillin and its associated proteins was determined in injured arteries by Western blot analysis. (ahajournals.org)
  • This neurotrophin-mediated decrease in the collapse response of DRG growth cones was not associated with reduced expression on growth cones of the Sema3A-binding protein neuropilin-1. (jneurosci.org)
  • receptors
  • Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. (genecards.org)
  • AT 1 receptors belong to the superfamily of seven-transmembrane-spanning-domain receptors and are coupled to a classical second messenger system via G-protein. (biologists.org)
  • role
  • As a member of the LIM domain-containing proteins, paxillin may play an important role in the differentiation of vascular SMCs. (ahajournals.org)
  • 12 13 SmCRP, an LIM protein cloned from rat aorta, has been found to play a role in the differentiation of vascular SMCs. (ahajournals.org)
  • contributes
  • 20-Hydroxyeicosatetraeonic acid (20-HETE) produced by cytochrome P -450 monooxygenases in NADPH-dependent manner is proinflammatory, and it contributes to the pathogenesis of systemic and pulmonary hypertension. (physiology.org)
  • apoptosis
  • Cell lysates were examined by immunoblots for EGF receptor and the MAP kinase ERK (both the activated [phosphorylated] and total protein is shown) and for apoptosis as measured by the presence of cleaved caspase. (ucdenver.edu)
  • effects
  • Several chapters also deal with some of the effects of cyclic GMP on other second messengers such as calcium ion transport and smooth muscle relaxation. (booktopia.com.au)
  • Conclusion- Our work thus defines AMPK╬▒1 as (1) a new kinase for RhoA and (2) a new mediator of the vasoprotective effects of estrogen. (ahajournals.org)
  • signal transduction
  • Paxillin is also a "docking" protein involved in organizing the signal transduction complex. (ahajournals.org)
  • Taken together, paxillin may function as a structural protein to maintain the integrity of the SMC contractile apparatus and as a docking protein to potentially mediate signal transduction for SMC contraction, migration, proliferation, and possibly differentiation. (ahajournals.org)
  • calcium
  • Protein phosphatases intervene in virtually all steps of neural modulation, and calcineurin, the sole calcium-activated phosphatase, is a key modulator for learning and memory ( Mansuy, 2003 ). (jneurosci.org)
  • mutant
  • Using wild type (wt) and non-myristoylated mutant PKG II proteins it was demonstrated that N-myristoylation (N-myr) is important for targeting of PKG II to membranes and distal ends of filopodia like structures in COS-1 and HEK-293 cells. (ubc.ca)
  • The calcineurin mutant tax-6 showed defective feeding state-dependent learning behavior for temperature and salt. (jneurosci.org)
  • Intestinal
  • Intestinal Fatty Acid Binding Protein is Associated with Mortality in Patients with Acute Heart Failure or Cardiogenic Shock. (bioportfolio.com)
  • Acute heart failure and cardiogenic shock are associated with an impaired intestinal perfusion, which may lead to a release of cytoplasmatic proteins by hypoxic epithelial injury. (bioportfolio.com)