• Our previous studies showed that NO causes cyclic GMP-independent arterial relaxation by increasing SERCA activity by inducing reversible S -glutathiolation at cysteine-674. (ahajournals.org)
  • Although redox regulation of SERCA by S -glutathiolation may cause acute cyclic GMP-independent arterial relaxation, it is unknown if redox-dependent regulation of SERCA is involved in more chronic effects of NO such as those on cell migration. (ahajournals.org)
  • We evaluated the ability of wild-type (WT) or R678X PRKG2 to regulate COL2 expression in cell culture. (uliege.be)
  • B) Co-transfection of PKG1β and HIV-1 infectious molecular clone, pNL4-3, resulted in dose dependent increase in viral protein expression. (biomedcentral.com)
  • Expression of FLAG-tagged transfected PKG1β (top panel) and HIV-1 viral proteins are shown by Western blotting. (biomedcentral.com)
  • Using HIV-specific hyper-immune sera in Western blots, we observed that PKG1β increased pNL 4-3 expression in a dose dependent manner (Figure 1B , lanes 2 - 6). (biomedcentral.com)
  • This study for the first time describes elevated levels of alpha synuclein in pancreatic adenocarcinoma as well as highlights the potential of evaluating NP protein expression. (antikoerper-online.de)
  • Methods and Results- To test our hypothesis, overexpression of either wild type (WT) or mutant SERCA in which cysteine-674 was mutated to serine was accomplished by stable transfection of HEK 293 or adenoviral expression in rat aortic smooth muscle cells (VSMCs). (ahajournals.org)
  • In the cell models expressing mutant SERCA, biotinylated-iodoacetamide (BIAM) and biotinylated-glutathione labeling of SERCA was decreased, and NO failed to increase SERCA activity or decrease Ca 2+ influx, thus validating that the expression of mutant SERCA prevents its redox-dependent activation. (ahajournals.org)
  • The vasodilator-stimulated phosphoprotein (VASP) 4 is a 46-kDa membrane-associated protein that is a member of the Enabled (Ena) family of proteins. (jimmunol.org)
  • Full length wt PKG II and wt regulatory PKG JJ proteins, when overexpressed in hippocampal neurons targeted to the synapse, however, a G2A PKG II mutant, which does not undergo myristoylation had a much more diffuse distribution in the entire cell and did not target to the synapse. (ubc.ca)
  • These proteins are localized to the actin cytoskeleton and are in vitro and in vivo ligands for the focal adhesion-associated proteins zyxin and vinculin and the actin-binding protein profilin ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ). (jimmunol.org)
  • IP3R-dependent calcium transients in the glutamatergic neurons convey this signal to downstream medial neurosecretory cells (mNSCs). (sdbonline.org)
  • cGMP-dependent protein kinase I beta physically and functionally interacts with the transcriptional regulator TFII-I". The Journal of Biological Chemistry. (wikipedia.org)
  • The effect of cGMP (cyclic GMP) dependent protein kinase 1-β (PKG1-β) and cGMP analogues on transcriptional activity and replication of human immunodeficiency virus type 1 (HIV-1) was investigated. (biomedcentral.com)
  • PKG1β was found to increase Tat dependent transcriptional activity of the HIV-1 LTR by four fold. (biomedcentral.com)
  • In this study, we explored the possible involvement of mechanosensitive Yes-associated protein (YAP) and its paralog, transcriptional coactivator with PDZ-binding domain (TAZ), transcriptional activation in extracellular matrix (ECM) production by LPA-induced contractile activity in human TM cells (HTM). (readbyqxmd.com)
  • VASP is believed to play an important role in controlling the cytoskeletal organization because it binds filamentous actin (F-actin) and profilin, a protein that forms complexes with G-actin and regulates actin dynamics ( 2 , 11 , 12 ). (jimmunol.org)
  • In contrast, blockers of the ADP-ribosyltransferase primarily reduced LTP, suggesting that NO, at least partly, acts via ADP-ribosylation of presynaptic proteins. (jneurosci.org)
  • Finally, preparation of caveolin-enriched lipid rafts from mouse lung and western blotting showed that GC-A and cGMP-dependent protein kinase I partly co-localize with Cav-1 in caveolae microdomains. (uni-wuerzburg.de)
  • Astrocytic endfeet are specialized cell compartments whose important homeostatic roles depend on their enrichment of water and ion channels anchored by the dystrophin associated protein complex (DAPC). (uio.no)
  • Moreover, PKG stimulation of Kir6.2/SUR2A channels in intact cells was abrogated by ROS/H(2)O(2) scavenging, antagonism of calmodulin, and blockade of calcium/calmodulin-dependent protein kinase II (CaMKII), respectively. (nih.gov)
  • PKG stimulation of K(ATP) channels was confirmed in intact ventricular cardiomyocytes, which was ROS- and CaMKII-dependent. (nih.gov)
  • Molecular cloning and predicted full-length amino acid sequence of the type I beta isozyme of cGMP-dependent protein kinase from human placenta. (wikipedia.org)
  • Based on previous studies it has been hypothesized that the molecular disassembly in astrocytic endfeet is caused by dystrophin cleavage mediated by Ca(2+) dependent proteases. (uio.no)
  • Viral proteins expressed from the HIV-1 molecular clone were then measured. (biomedcentral.com)
  • In the absence of NO, fetal bovine serum stimulated migration of both cell types expressing WT or C674S SERCA at similar rates. (ahajournals.org)
  • Using wild type (wt) and non-myristoylated mutant PKG II proteins it was demonstrated that N-myristoylation (N-myr) is important for targeting of PKG II to membranes and distal ends of filopodia like structures in COS-1 and HEK-293 cells. (ubc.ca)
  • However, compared to wild-type controls, the magnitude and duration of ORN Ca2+-response was significantly diminished in cAMP-defective flies. (sdbonline.org)
  • Growth cone retraction mediated by Sema3A is overcome by cGMP only in wild-type, but not in p53 -null dorsal root ganglia. (nih.gov)
  • This C/T transition introduced a stop codon (R678X) that truncated 85 C-terminal amino acids, including a large portion of the kinase domain. (uliege.be)
  • Background: Diabetes mellitus type 2 (DM2) is highly associated with increased risk for chronic kidney disease (CKD), end stage renal disease (ESRD) and cardiovascular morbidity. (uni-wuerzburg.de)