• abstract = "Pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) type L was partly purified from rat kidney. (elsevier.com)
  • The PKR1 gene encoding the protein kinase A (PKA) regulatory subunit was also identified and disrupted. (asm.org)
  • This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • The regulatory subunit of neural cAMPdependent protein kinase II represents a unique gene product. (springer.com)
  • Our results suggest that cAMP stimulates CREB-dependent gene transcription via a two-step process by inducing release of an excitatory amino acid, identified here as l -aspartate, which then activates NMDARs, leading to Ca 2+ entry and gene transcription. (jneurosci.org)
  • Although highly homologous within their kinase domains (98% identity), the two gene products share only 36% identity in the last 76 C-terminal residues ( Woodgett, 1990 ). (biologists.org)
  • A gene on chromosome 5q35.1 that encodes G protein-coupled dopamine receptor D1, which is the most abundant dopamine receptor in the CNS. (thefreedictionary.com)
  • Stathmin, also known as metablastin and oncoprotein 18 is a protein that in humans is encoded by the STMN1 gene. (wikipedia.org)
  • In addition, we show here that cAMP repression applies to both Msn2/4p-dependent and -independent control of gene expression at the diauxic shift. (asm.org)
  • Major changes in the pattern of gene expression occurring during this transition have been characterized by analysis of the protein pattern obtained by two-dimensional (2-D) gel electrophoresis. (asm.org)
  • We demonstrate that σ32 inhibits growth and that its unrestrained activity leads to an extensive reprogramming of global gene expression, resulting in upregulation of repair and maintenance functions and downregulation of the growth-promoting functions of protein translation, DNA replication and certain metabolic processes. (bireme.br)
  • We examined the morphogenetic behavior of C. albicans yeast cells lacking the BCY1 gene, which encodes the regulatory subunit of protein kinase A. We cloned the BCY1 gene and generated a bcy1 tpk2 double mutant strain because a homozygous bcy1 mutant in a wild-type genetic background could not be obtained. (asm.org)
  • In the bcy1 tpk2 mutant, protein kinase A activity (due to the presence of the TPK1 gene) was cyclic AMP independent, indicating that the cells harbored an unregulated phosphotransferase activity. (asm.org)
  • Mitochondrial contents, expression, and activation status of p38 mitogen-activated protein kinase (MAPK) and PPARγ coactivator 1α (PGC-1α) were compared between skeletal muscle samples from adiponectin gene knockout, adiponectin-reconstituted, and control mice. (diabetesjournals.org)
  • While Gα olf expression was not detectable in β-cells, experiments using inhibitors and siRNA revealed that the pathway dependent on phospholipase C-inositol triphosphate, rather than cAMP-protein kinase A, mediates GSIS potentiation via OLFR15. (nature.com)
  • The inactivation is stable during purification in the presence of protein phosphatase inhibitors, and is associated with a 30-40% increase in the labelling of enzyme isolated from 32P-labelled cells. (duke.edu)
  • Retinoid acid increases cyclic amp-dependent protein kinase activity in b16-f1 mouse melanoma cells. (jax.org)
  • Niles, R M. and Logue, M P., "Retinoid acid increases cyclic amp-dependent protein kinase activity in b16-f1 mouse melanoma cells. (jax.org)
  • The phosphorylation of VASP was dependent on cell spreading, as VASP was expressed as a dephosphorylated protein in round adherent cells and was phosphorylated at the onset of changes in cell shape from round to spread cells. (jimmunol.org)
  • refs 14,15), neither Ser376 dephosphorylation, nor the interaction of p53 with 14-3-3 proteins occurred in AT cells. (nih.gov)
  • This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. (cancerindex.org)
  • In this study, we have directly visualized Ca(2+)-dependent exocytosis of both LVs and SVs with two-photon imaging in mouse pancreatic beta-cells. (biomedsearch.com)
  • We also detected FRET between CaNA and PKA-RII bound simultaneously to AKAP79 within 50 Å of each other, thus providing the first direct evidence of a ternary kinase-scaffold-phosphatase complex in living cells. (rupress.org)
  • Unfortunately, the biochemical precipitation techniques used to study the assembly of protein complexes are limited by steric hindrance from bulky fusion proteins or antibodies, use of detergents to solubilize complexes from cells, and poor yield of proteins precipitating through intermediate proteins. (rupress.org)
  • Depletion of HeLa cell brefeldin A-inhibited guanine nucleotide-exchange factors (BIG)1 and/or BIG2 impaired β-catenin S675 phosphorylation and its transcriptional effects, as well as ADP-ribosylation factor activation-enhanced phospholipase D activity and vesicular trafficking, both required for restoration of transcriptional activation by appropriate BIG protein overexpression in depleted cells. (pnas.org)
  • Cyclic AMP-dependent protein kinase in human embryonic palate mesenchymal cells. (nih.gov)
  • These protein complexes mediate the adhesive properties of particular cells, the formation of the paracellular barrier (tight junctions), ion transport, and transmission of signals between adjacent cells that regulate growth, differentiation, and homeostasis. (jci.org)
  • We observed co-precipitation of G-protein-coupled receptor kinases (GRKs) and caveolin-1 with Rec from cell lysates of 293 or SSTW cells. (curehunter.com)
  • Central to its antidiuretic action in mammals is the redistribution of the water channel aquaporin 2 ( AQP2 ) from intracellular vesicles to the apical membrane of kidney epithelial cells , an event initiated by an increase in cAMP and activation of protein kinase A . The subsequent steps of the signaling cascade are not known. (ndif.org)
  • Aquaporin-2 is a water-transporting protein located in the principal cells of the kidney collecting duct . (ndif.org)
  • Wanting to further clarify which of the G proteins were involved in AQP2 vesicle transport , the researchers introduced different synthetic peptides at different times into the CD8 cells , then analyzed their affects on AQP2 movement from ER to cell surface . (ndif.org)
  • B) Concentration-dependent effect of cAMP on the EC50 for IP3-evoked Ca2+ release from permeabilized HEK-PR1 cells. (nih.gov)
  • Van Kolen, Slegers: Atypical PKCzeta is involved in RhoA-dependent mitogenic signaling by the P2Y(12) receptor in C6 cells. (antibodies-online.com)
  • Induction of Cbeta splice variants and formation of novel forms of protein kinase A type II holoenzymes during retinoic acid-induced differentiation of human NT2 cells. (uio.no)
  • RESEARCH DESIGN AND METHODS Using matrix-targeted photoactivatable green fluorescent protein, mitochondria were tagged and tracked in β-cells within intact islets, as isolated cells and as cell lines, revealing frequent fusion and fission events. (diabetesjournals.org)
  • Cells were exposed to arachidonic acid (AA) plus Fe, which has been previously reported to cause a synergistic toxicity in E47 cells by a mechanism dependent on CYP2E1 activity and involving oxidative stress and lipid peroxidation. (aspetjournals.org)
  • The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. (cancerindex.org)
  • These domains are, figuratively speaking, the glue that binds protein complexes together, and their unique specificity and regulated binding determine the distinct compositions of different complexes. (jci.org)
  • 1 The receptor for u-PA (u-PAR) is a 55-65 kDa glycosylphosphatidylinositol-linked protein that binds the epidermal growth factor-(EGF)-like domain (amino acids 20-32) of two chain u-PA. (bmj.com)
  • After incubation of the phosphorylated kidney pyruvate kinase with phosphatase the phosphoenolpyruvate saturation curve was found to be identical to that for the unphosphorylated enzyme. (elsevier.com)
  • Then, the NAD(P)H dependent enzyme dihydropteridine reductase (DHPR) converts q-BH 2 back to BH 4 . (wikipedia.org)
  • These include the structural basis for specificity of their binding interactions and ideas about how they organize both small local protein complexes used for signal transduction (transducisomes) and larger two-dimensional complexes like cell junctions and plasma membrane domains. (jci.org)
  • Interactions mediated by domains that appear in a large number of proteins are of particular interest since they are expected to have an impact on diversities of cellular processes such as signal transduction and immune response. (biomedcentral.com)
  • The fraction containing Erk2, as well as bacterially expressed Erk1 and Erk2, phosphorylated all types of KSP motifs in peptides (KSPXK, KSPXXK, KSPXXXK, and KSPXXXXK) derived from NF-M and NF-H. They also phosphorylated an expressed 24 KSPXXXK repeat NF-H polypeptide, an expressed NF-H as well as dephosphorylated native rat NF-H, and NF-M proteins with accompanying decreases in their respective electrophoretic mobilities. (jneurosci.org)
  • The peptides corresponded to a part of the G proteins called the C-terminus (the tail end of the protein ). (ndif.org)
  • We also found that RGS13 protein degradation is protected by PKA phosphorylation on Thr41 residue, while RGS13 mRNA transcription was negatively regulated by the tumor suppressor p53. (nih.gov)
  • Binary signaling requires localized delivery of cAMP, whereas analogue signaling is more dependent on localized cAMP degradation. (nih.gov)
  • Most notably, we describe a mutation in the ATP dependent protease HslUV that induces rapid degradation of σ32, and a mutation leading to increased levels of the house keeping σ70 that outcompete σ32 for binding to the RNA polymerase. (bireme.br)
  • Inhibition of GSK-3β either by small molecules (SB216763 or SB415286) or by ectopic expression of kinase-inactive GSK-3β before irradiation significantly attenuated radiation-induced apoptosis in hippocampal neurons. (aacrjournals.org)
  • In five-month-old mice (left) expressing mutant tau (red), cortical neurons largely clear a ubiquitinated protein (green), but by eight months the tagged protein accumulates. (alzforum.org)
  • The existence of eukaryotic CNB domains not associated with binding of cyclic nucleotides suggests that orphan CNB domains in other eukaryotes may bind undiscovered signalling molecules. (nature.com)
  • In eukaryotes, CNB domains are bound and regulated by cyclic nucleotides. (nature.com)
  • Stable isotope labeling was used to confidently identify and differentially quantify target proteins and their preferential binding affinity for the three different cAMP analogs. (mcponline.org)
  • Long-term memory in Drosophila is separable into two components: consolidated, anesthesia-resistant memory and long-lasting, protein-synthesis-dependent memory. (pnas.org)
  • i ) resistance to treatments such as anesthesia or electroconvulsive shock that disrupt the patterned activity of the brain ( 1 ), and ( ii ) requirement for new protein synthesis ( 2 , 3 ). (pnas.org)
  • Anesthesia-resistant memory (ARM) is deficient in the memory mutant radish , whereas long-lasting protein-synthesis-dependent memory is eliminated by overexpressing the blocking form of the transcription factor cAMP-response element-binding protein or by feeding cycloheximide ( 4 , 5 , 6 ). (pnas.org)
  • ARM and long-lasting protein-synthesis-dependent memory may interact at times, at least in anatomically mutant flies ( 7 ). (pnas.org)
  • The specific defect of the radish mutant, diminished ARM, normal long-lasting protein-synthesis-dependent memory, provides a key to obtaining mechanistic information about ARM. (pnas.org)
  • We analyzed the two-dimensional gel electrophoresis pattern of protein synthesis of the msn2 msn4 double mutant and compared it with that of the wild-type strain during exponential growth phase and at the diauxic transition. (asm.org)
  • Zhang, Keane, Zhu, Sharma, Rozengurt, Strieter, Dubinett, Huang: Interleukin-7 and transforming growth factor-beta play counter-regulatory roles in protein kinase C-delta-dependent control of fibroblast collagen synthesis in pulmonary fibrosis. (antibodies-online.com)
  • eEF1A1 is associated with ribosome activity and protein synthesis and brings the incoming aminoacyl-tRNA in a GTP-dependent manner to a site within the ribosome referred to as the aminoacyl (A) site. (uio.no)
  • Our data implies that we have identified a novel binding partner for the C subunit of PKA expressed in all tissues except differentiated skeletal muscle, linking the regulatory role of the cAMP-PKA signalling pathway to protein synthesis. (uio.no)