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To investigate in vivo molecular mechanisms of silibinin efficacy, tumors and uninvolved skin from tumor-bearing mice were examined immunohistochemically for proliferation, p53, apoptosis, and activated caspase-3. (nih.gov)
Together, these results show a strong preventive efficacy of silibinin against photocarcinogenesis, which involves the inhibition of DNA synthesis, cell proliferation, and cell cycle progression and an induction of apoptosis. (nih.gov)
Galectins are β-galactoside-binding lectins that are involved in various biologic processes, such as apoptosis, cell proliferation, and cell-cycle regulation. (elsevierpure.com)
The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. (wikipedia.org)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
Cell proliferation was measured and the linear portion of the curve was used to calculate the ED 50 . (cellsignal.com)
The bioactivity of recombinant mIL-11 was determined in a B9 cell proliferation assay. (cellsignal.com)
Interleukin-11 (IL-11) was initially cloned as a mediator of plasmacytoma cell proliferation and was later found to exhibit a wide variety of biological effects in neural cells as well as in the hematopoietic and immune systems (1). (cellsignal.com)
Treatment of LMP1/CD40-expressing lymphomatous mice with an anti-PD-L1 monoclonal antibody induced tumor regression with decreased spleen content, activation and proliferation rate of B-cells as well as a marked increase in T-cell activation, as assessed by CD62L and CD44 expression. (springer.com)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
In bladder cancer (BCa), there is emerging evidence that BCa CAFs are actively involved in cancer cell proliferation, invasion, metastasis, and chemotherapy resistance. (bvsalud.org)
The overexpressed and knockdown plasmids were used to evaluate the effect of circGUCY1A2 on NSCLC cell proliferation and apoptosis efficacy. (bvsalud.org)
CircGUCY1A2 upregulation promoted apoptosis and inhibits cell proliferation and growth of subcutaneous tumorigenicity grafts in nude mice (P (bvsalud.org)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Heterozygous mutation of Trp53 decreases p53 and results in attenuated apoptosis and G1-S checkpoint control, allowing Brca1Delta11/Delta11 cells to proliferate. (nih.gov)
Reactivation of p53 in established tumors typically results in one of two cell fates, cell cycle arrest or apoptosis, but it remains unclear how this cell fate is determined. (nih.gov)
We hypothesized that high mitochondrial priming prior to p53 reactivation would lead to apoptosis, while low priming would lead to survival and cell cycle arrest. (nih.gov)
Using a panel of Kras-driven, p53 restorable cell lines derived from genetically engineered mouse models of lung adenocarcinoma and sarcoma (both of which undergo cell cycle arrest upon p53 restoration), as well as lymphoma (which instead undergo apoptosis), we show that the level of mitochondrial apoptotic priming is a critical determinant of p53 reactivation outcome. (nih.gov)
Cells with high initial priming (e.g., lymphomas) lacked sufficient reserve antiapoptotic capacity and underwent apoptosis after p53 restoration. (nih.gov)
the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
Objective: To investigate the effects of prion protein 106-126 peptide on inducing apoptosis in differentiated PC12 cells. (researchgate.net)
Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
If cells containing damaged DNA were to divide, the errors would be transmitted to daughter cells, generating genomic instability and resulting in tumorigenesis or apoptosis . (tocris.com)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
In SCO syndrome mouse model, PRPS2 overexpression significantly inhibited cell apoptosis and promoted cell cycle transition in TM4 Sertoli cells. (medscape.com)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)

Developmental changes in interkinetic nuclear migration dynamics with respect to cell-cycle progression in the mouse cerebral cortex ventricular zone. (sbri.fr)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Cell-cycle distribution analyses revealed that Gal-9 maintained further progression of cell cycle from the G1 phase. (elsevierpure.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Checkpoint control kinases is a term used to describe a group of enzymes that regulate progression of a cell through the cell cycle. (tocris.com)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
Ovol1 and Ovol2, a family of zinc finger transcription factors, are expressed in spermatocytes at the pachytene stage and are suggested to be critical regulators of pachytene progression in male germ cells. (medscape.com)
Cancer-associated fibroblasts (CAFs) are key stromal cells in the tumor microenvironment (TME) that critically contribute to cancer initiation and progression. (bvsalud.org)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

Jovic D, Haque A, Hexig B, Nagaoka M, Akaike T (2011) Control of Singular Cell Cycle Synchronization of Mouse ES Cells for Hepatocyte Differentiation on E-Cadherin Substratum. (omicsonline.org)
Moreover, synchronized cells re-enter into the normal cell cycle with the elimination of hydroxyurea for differentiation. (omicsonline.org)
Our strategy for ES cell cycle synchronization before differentiation induction possibly helps to increase the yield of hepatocyte-like cells under homogeneous culture condition. (omicsonline.org)
Is the Subject Area "Cell differentiation" applicable to this article? (plos.org)
Myogenin also identified as myogenic factor 4 is a muscle-specific transcription factorassociated with muscle differentiation and cell cycle. (sigmaaldrich.com)
Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
Furthermore, we used this assay to determine the effects of BRCA1 variants on cell cycle regulation, differentiation, and genomic stability. (jci.org)

However, most female Brca1Delta11/Delta11 Trp53+/- mice develop mammary tumors with loss of the remaining Trp53 allele within 6-12 months. (nih.gov)
Xpc −/− mice displayed a significant increase in lung tumors and a trend toward increased liver tumors compared with Xpa -deficient or wild-type mice. (aacrjournals.org)
Xpa −/− mice showed a significant elevation in liver tumors. (aacrjournals.org)
Unlike cancer arising in the human population, tumors in genetically engineered mouse models arise in mice with well defined genetic backgrounds where genetic variability can be minimized. (molvis.org)
Investigators at the Icahn School of Medicine at Mount Sinai have designed an innovative RNA-based strategy to activate dendritic cells - which play a key role in immune response - that eradicated tumors and prevented their recurrence in mouse models of melanoma. (worldpharmanews.com)
This immunosuppressive environment that impedes activation of cancer-killing T cells allows tumors to grow, say the researchers. (worldpharmanews.com)
This is because tumors have a tendency to evolve in different ways to switch off each stage of the cancer-immunity cycle," says Yizhou Dong, PhD, corresponding author of the study, Professor of Oncological Sciences, and a member of the Icahn Genomics Institute and the Marc and Jennifer Lipschultz Precision Immunology Institute at Icahn Mount Sinai. (worldpharmanews.com)
As part of the regimen, the researchers used new types of lipid nanoparticles to deliver two mRNA therapeutics - a process similar to that used successfully for COVID-19 vaccines - to ensure the dendritic cells were sufficiently activated to enhance the cancer-immunity cycle in established tumors. (worldpharmanews.com)
This caused a change in the tumor's microenvironment, shifting it from having cell types that weaken T cells' ability to fight cancer to having cell types that actually support and enhance their ability to fight tumors. (worldpharmanews.com)
Their investigations revealed encouraging results, as the regimen reduced tumors in mouse models of B cell lymphoma by 83 percent. (worldpharmanews.com)
In theory, the CATCH regimen using this particular RNA-based technology has the potential to provide a much more effective approach for using dendritic cells for cancer immunotherapy to treat a wide range of solid tumors," says Brian Brown, PhD, Director of the Icahn Genomics Institute and Associate Director of the Marc and Jennifer Lipschultz Precision Immunology Institute at Icahn Mount Sinai. (worldpharmanews.com)
LMP1/CD40 tumors B-cells expressed high levels of PD-L1. (springer.com)
[ 2 ] Targeted disruption of TP53 in the mouse leads to the development of various tumors (see image below). (medscape.com)
Tumors that developed in mice deficient in INK4A were enhanced by the topical application of carcinogens and ultraviolet light. (medscape.com)

In this study, the effect of Gal-9 on Gl-phase cell-cycle arrest, one of the hallmark pathologic changes in early diabetic nephropathy, was investigated. (elsevierpure.com)
Forced BCL-2 or BCL-XL expression reduced priming and resulted in survival and cell cycle arrest. (nih.gov)
Cells with low initial priming (e.g., lung adenocarcinoma and sarcoma) survived and proceeded to arrest in the cell cycle. (nih.gov)
Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)

For further confirming the cell-cycle regulation by Gal-9, conditionally immortalized mouse podocyte cells were cultured under 5.5 and 25 mM D-glucose supplemented with Gal-9. (elsevierpure.com)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. (tocris.com)
The participation of autophagy in hemopoietic stem cell regulation in mice Knockouts. (usp.br)
Although several studies have examined the protective mechanisms of dietary EA including the induction of detoxifying enzymes, regulation of cell cycle, chelation of nickel, and prevention of DNA methylation, none have addressed the role of EA in immunological surveillance. (cdc.gov)

To elucidate differences in the functions of these two proteins, comprehensive survival studies with Xpa −/− , Xpc −/− and wild-type control female mice in a pure C57BL/6J background were done. (aacrjournals.org)
This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
This pathway stimulates the production of proteins important in alerting the stem cells. (stanford.edu)
All taken together, these observations underline the complexity of the mechanisms responsible for the selective destruction of proteins within cells. (cnrs.fr)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Mice Swiss males, adults, had been fed or with ration I contend 2% of protein (unfed group, D) or with ration with 20% of protein (group has controlled, C). The used protein source was casein. (usp.br)
In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. (frontiersin.org)
ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Methods: After differentiated by nerve growth factor (NGF), the PC12 cells were infected by prion protein 106-126 peptide. (researchgate.net)
Serial dilutions of Mouse IL-11 Recombinant Protein were added to B9 cells. (cellsignal.com)
The purity of Mouse IL-11 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mIL-11 and staining with Coomassie Blue. (cellsignal.com)
Mouse IL-11 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. (cellsignal.com)
Pretreatment with a stem-cell-activating protein significantly enhances healing in mice, Stanford researchers say. (stanford.edu)
Like drag car racers revving their engines at the starting line, stem cells respond more quickly to injury when they've been previously primed with one dose of a single protein, according to a study from the Stanford University School of Medicine . (stanford.edu)
Mice given the priming protein recover muscle function more quickly after damage, their skin heals more rapidly and even the shaved area around the injury regrows hair more quickly, the study found. (stanford.edu)
Harnessing the power of this protein may one day help people recover more quickly from surgery or restore youthful vigor to aging stem cells. (stanford.edu)
Rodgers and his colleagues found that a protein called hepatocyte growth factor, which exists in a latent form in the spaces between muscle cells and tissue, can activate a critical signaling pathway in the cells by binding to their surfaces. (stanford.edu)
TSEs are characterized by the accumulation in the central nervous system and, less often, in lymphoid tissues of TSE-associated prion protein (PrP TSE ), a conformational variant of a normal host cellular prion protein (PrP C ). PrP C is a nonessential protein but, at least in mice and cows, must be expressed by animals susceptible to TSE infection. (cdc.gov)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green ¯uorescent protein (GFP)-expressing cells. (lu.se)
The aggregation of the protein Amyloid-beta (Abeta) into plaques outside the nerve cells has been recognized in patients with Alzheimer's disease since 1905. (lu.se)
Hence, plaques sucking Abeta out of the cells may trigger even more production of that protein in a vicious cycle. (lu.se)

The lungs of the mice were monitored for transcriptomic changes on a genome-wide level, which confirmed an impaired expression of antioxidant and phase 2-related genes in CS-exposed Nrf2(-/-) mice. (pmiscience.com)
Results Primary B cells exogenously expressing Bcl2 , Myc and Ccnd1 showed factor-independent growth ability, enhanced colony-forming capability and aggressive oncogenicity, unlike the cases observed with the expression of any combination of only two of the genes. (haematologica.org)
These genes are transcriptionally deregulated as the partner genes of IgH translocation, and are thus thought to perform crucial roles in human B-cell lymphomagenesis. (haematologica.org)
2 A synergistic effect resulting from a combination of two genes from BCL2 , MYC and CCND1 has been implicated in human B-cell lymphomagenesis. (haematologica.org)
7 Importantly, it is believed that other hitherto unknown genes also play important roles in lymphomagenesis in addition to the synergistic effects of the aforementioned two oncogenes since human B-cell lymphomas often show a variety of genes subject to alterations and/or deregulated expression. (haematologica.org)
7 That multiple genes are involved in human lymphoma formation has also been suggested by experimental mouse models. (haematologica.org)
In humans, the wide variety of carcinogens and varying degrees of exposure make identifying the predisposing genes difficult, but in a mouse model, such confounding variables can be controlled. (bmj.com)
Identification of the genes predisposing to mouse lung cancer could have considerable implications for diagnosis, treatment, or chemoprevention of lung cancer in humans. (bmj.com)
Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). (rndsystems.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)

Journal of Cell Biology (2019) 218 (9): 2854. (biologists.com)
Journal of Cell Biology (2018) 217 (10): 3416. (biologists.com)
Journal of Cell Biology (2017) 216 (10): 3133. (biologists.com)
Rando, who also directs Stanford's Glenn Center for the Biology of Aging , is the senior author of the study, published April 18 in Cell Reports . (stanford.edu)
Rodgers is now an assistant professor of stem cell biology and regenerative medicine at the University of Southern California. (stanford.edu)
Frontiers in cell and developmental biology 2021 9 698558. (cdc.gov)

Eight-week-old male db/db mice received injections of recombinant Gal-9 or vehicle for 8 wk. (elsevierpure.com)
In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed. (rndsystems.com)
Recombinant mouse IL-11 was expressed in E. coli and is supplied in a lyophilized form. (cellsignal.com)

More importantly, we show that MESF treatment leads to significant inhibition of tumor growth and a 3-fold increase in the life span of tumor bearing animals compared to untreated tumor mice. (nature.com)
We could confirm the increased polyploidy upon Mps1 inhibition in neuroblastoma cell lines and PDX model, leading to mitotic catastrophe activating the caspase-dependent mitochondrial apoptotic pathway. (lu.se)
In addition, Mps1 inhibition decreased tumour growth in a xenograft mouse model. (lu.se)
Inhibition of pro-inflammatory myeloid cell responses by short-termS 100A9 blockade improves cardiac function after myocardial infarction. (lu.se)

However, preparation of specific lineages at high purity from embryonic stem cells remains a challenge. (omicsonline.org)

Using multiple bioassays to gain insights on the effects of the CATCH regimen on different types of immune cells, the researchers showed that their strategy not only reactivated the cycle but also removed obstacles at other stages. (worldpharmanews.com)
Escape from immune control must be important in the natural course of B-cell lymphomas, especially for those with activation of NF-κB. (springer.com)
As reviewed recently, various publications have indicated that disruption of immune checkpoints is also a critical step in B-cell non-Hodgkin's Lymphomas (NHL) [ 3 ]. (springer.com)
Here, we wanted to explore the putative interest of PD-L1 immune therapy against B-cell lymphoma with NF-κB activation. (springer.com)
Spleen from mice were collected and immune cells were filtered through a sterile nylon membrane. (springer.com)
This study investigates the status of immune function in B6C3F1 mice exposed continuously to EA in drinking water at 0.5, 1.0, or 2.0 mg/kg/day for 28 days. (cdc.gov)
There were also significant differences in microbial composition in dust samples and in innate immune cells between the two groups of children. (medscape.com)

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. (cellsignal.com)
The pre-clinical LMP1/CD40-expressing transgenic mouse model is characterized by B-cell specific CD40 signaling responsible for NF-κB continuous activation with a spleen monoclonal B-cell tumor after 1 year in 60% of cases. (springer.com)
FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)

Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate. (sigmaaldrich.com)
Immunological parameters assessed included natural killer (NK) cell activity, cytotoxic T lymphocyte (CTL) activity, IgM antibody plaque forming cell (PFC) response, thymus, spleen, kidney, and liver mass, and total cellularity for the thymus and spleen. (cdc.gov)
Subchronic exposure to EA for 28 days in drinking water caused significant suppression of specific IgM antibody responses in the 2.0 mg/kg EA treatment group and suppressed cytotoxic T-cell function in the 0.5 and 1.0 mg/kg EA treatment groups. (cdc.gov)

Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)

In this work we evaluate the effect of the DP on the cellular cycle of cells of bone marrow of mice. (usp.br)
After loss of 25% of corporeal weight of group D, harvested blood and cells of bone marrow and spleen. (usp.br)
The cells of the blood had been quantified as well as the ones of spleen and bone marrow. (usp.br)
The cells of the bone marrow later had been coradas with orange of acridine (AO) and propidium iodide of (PI), evaluating, for citometria of flow, respectively DNA/RNA and DNA. (usp.br)
Hematopoietic stem/progenitor cell cycle evaluation from bone marrow of malnourished. (usp.br)
We've shown that muscle and bone marrow stem cells enter a stage of alertness in response to distant injury that allows them to spring into action more quickly. (stanford.edu)

Design and Methods Growth in culture, colony formation and oncogenicity in vivo were assessed in mouse primary B cells exogenously expressing various combinations of Bcl2 , Myc and Ccnd1 . (haematologica.org)
In this issue of the JCI , Chang, Sharan, and colleagues describe a novel system to evaluate human BRCA1 alleles for in vivo function using BACs containing human BRCA1 vectors in mouse cells and embryos (see the related article beginning on page 3160). (jci.org)
This expression was dependent on activation of either NF-κB, JAK1/JAK2 or BTK pathways since these pathways were activated in tumor B-cells and ex vivo treatment with the inhibitory molecules PHA-408, ruxolitinib and ibrutinib led to decrease of its expression. (springer.com)
Contrast enhanced MRI on cochlea of the mouse inner ear in vivo imaging acquired using a Bruker Cryocoil. (lu.se)
In vivo imaging of the mouse brain using a Bruker Cryocoil. (lu.se)

Cyclin dependent-kinase 2 (CDK2) plays important functions during the mitotic cell cycle and also facilitates several key events during germ cell development. (lu.se)
This offers significant advantages for studying tumor pathogenesis and molecular mechanisms of oncogenesis caused by a single initiating oncogenic event introduced through the mouse germ line. (molvis.org)
Sertoli-cell-only (SCO) syndrome, also called germ cell aplasia or Del Castillo syndrome, describes a condition of the testes in which only Sertoli cells line the seminiferous tubules. (medscape.com)
A congenital absence of germ cells due to failure of migration of gonocytes is theoretically possible. (medscape.com)

We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)

The injection of Gal-9 into db/db mice significantly inhibited glomerular hypertrophy and mesangial matrix expansion and reduced urinary albumin excretion. (elsevierpure.com)
Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)

NF-κB, one of the most cited transcription factor in B-cell lymphomas, is able to increase tumor cell expression of PD-L1 either directly or indirectly [ 3 ]. (springer.com)
Taniguchi and colleagues reported that while hOvol1 and hOvol2 were detected by reverse transcription-polymerase chain reaction (RT-PCR) in the testes of patients capable of spermatogenesis, they were not detected in those with Sertoli cell-only syndrome. (medscape.com)
As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)

02:47 and red blood cells in vitro. (yale.edu)
NV556 treatment in an in vitro 3D human liver Extracellular Matrix (ECM) cultured with a TGFβ1-activated human hepatic stellate cell line led to decreased collagen production. (lu.se)

Background A synergistic effect resulting from a combination of BCL2 and MYC or MYC and CCND1 has been implicated in human B-cell lymphomas. (haematologica.org)
Genetic alterations of BCL2 (B-cell CLL/lymphoma 2), MYC [v-myc myelocytomatosis viral oncogene homolog (avian)] and CCND1 (cyclin D1) are the most frequently found alterations in human B-cell lymphomas. (haematologica.org)
NF-κB constitutive activation is found either in aggressive diffuse large B-cell lymphomas (DLBCL) with an activated phenotype (ABC-DLBCL), or in indolent B-cell lymphomas such as chronic lymphocytic leukemia, Waldenström Macroglobulinemia, marginal zone B-cell lymphomas (MZL) [ 4 ]. (springer.com)

Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit. (antikoerper-online.de)
Cell viability was determined by using the MTT assay and the morphological changes were observed. (researchgate.net)
The combination of NV651 and cisplatin, a DNA damage reagent, resulted in a synergistic effect in cell viability, and a significant increase on cell death in HCC cell lines. (lu.se)

Gal-9 reversed the high-glucose-mediated upregulation of p27 Kip1 and p21 Cip1 and inhibited cell-cycle-dependent hypertrophy, i.e., reduced [ 3 H]proline incorporation. (elsevierpure.com)
We also demonstrated upregulation of ETS-1 and ETS-2 target expressions in Tyrp-1-TAg mice when comparing with the same target expressions in control mice. (molvis.org)

Cell Rep, 2019 Sep 10. (nih.gov)
Developmental Cell (2019) 51 (2): 145. (biologists.com)

We looked at this both in cultured cell lines as well as in mouse models carrying mutations in the Abeta gene. (lu.se)
We then looked in mice with AD mutations where we also had accelerated the Abeta aggregation and found lots of aggregated Abeta inside the neurons at the first time points. (lu.se)

Methods: The human multiple myeloma SKO-007 cells in logarithm growth phase were selected and randomly divided into control group, LDM group, BZM group, and BZM combined with LDM group. (researchgate.net)

Previously, classical genetic studies involving cross breeding of mouse strains with differing susceptibilities have identified chromosomal areas associated with predisposition to developing spontaneous and chemically induced lung adenomas. (bmj.com)
6 Inbred mouse strains vary in their susceptibility to cancer and two extreme strains are A/J (susceptible) and C57BL/6J (B6, resistant). (bmj.com)
Mouse pulmoary adenoma susceptibility loci (Pas) as mapped in crosses of strains A/J and C57BL/6. (bmj.com)

Importantly, in comparison to wt mice, an attenuated cell cycle/mitotic response and intensified stress gene expression pattern were observed in exposed Nrf2(-/-) mice, which was paralleled by clear dose-dependent effects on alveolar destruction and impaired lung function. (pmiscience.com)
The objective of this study was to identify additional cooperative gene(s) associated with BCL2 and MYC or MYC and CCND1 in human B-cell lymphomagenesis. (haematologica.org)
Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family. (frontiersin.org)
Based on these findings, the team now returns with a new study demonstrating that giving a mouse just a little more shelterin (by TRF1 gene therapy) promotes extended healthy aging! (epigenie.com)
The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. (lu.se)

Recent studies demonstrate that similar epigenetic clocks for mice (Mus Musculus) can be slowed by gold standard anti-aging interventions such as calorie restriction and growth hormone receptor knock-outs. (sens.org)
We show that, while the axon guidance molecule Netrin-1 promotes nerve invasion by blood vessels via the endothelial receptor UNC5B during embryogenesis, myelinated Schwann cells negatively control intra-nervous vascularization during post-natal period. (elifesciences.org)
IL-11 acts on cells expressing gp130 and the IL-11 receptor (IL-11R) α subunit. (cellsignal.com)
1994. Ah receptor in embryonic mouse palate and effects of TCDD on receptor expression. (cdc.gov)

Past studies have mapped four susceptibility loci (Pas1-4) for pulmonary adenoma in which A/J and C57BL/6J (B6) mice have different alleles that affect incidence and multiplicity of tumours. (bmj.com)

For a cell to progress through the cycle and replicate, it must pass through checkpoints between phases to ensure that DNA is replicated correctly and that chromosomes segregate. (tocris.com)
These checkpoints may stop the cell cycle after DNA damage, loss of DNA replication or disruption of the mitotic spindle, in order for repair processes to take place. (tocris.com)

During early stages of embryogenesis, by a process called vasculogenesis, a part of the mesoderm differentiates into endothelial cells, forms a lumen, and deposits a basal lamina to create a vascular plexus de novo. (elifesciences.org)
This mechanism involves specialized endothelial cells named tip cells. (elifesciences.org)
VEGF is a highly specific mitogen for vascular endothelial cells. (sigmaaldrich.com)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)

Drinking grape juice 30 minutes before meals for a week following each cycle of chemotherapy doesn't seem to reduce nausea or vomiting caused by chemotherapy. (medlineplus.gov)

It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death. (tocris.com)

Galectin-9 (Gal-9) was identified previously and demonstrated to have apoptotic potential to thymocytes in mice and activated CDS+ T cells in nephrotoxic serum nephritis model. (elsevierpure.com)
Mitochondrial apoptotic priming is a key determinant of cell fate upon p53 restoration. (nih.gov)
Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. (researchgate.net)

Conclusions Bcl2 , Myc and Ccnd1 or Bcl2 , Myc and CCND3 synergistically transformed mouse primary B cells into aggressive malignant cells. (haematologica.org)

Later, when Abeta also accumulates in critical amounts outside the cells, this may suck out the internal Abeta, like osmosis of molecules over a membrane to have equal concentrations on both sides. (lu.se)

Importantly, human B-cell lymphoma cases with concurrent multiple translocations including BCL2 , MYC and CCND1 have been reported. (haematologica.org)
Importantly, we discovered that ES cells rescued by S1497A BRCA1 exhibited significant hypersensitivity after γ-irradiation. (jci.org)

In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)

MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination. (nih.gov)
Here, we sought to elucidate the underlying mechanism(s) using mice deficient in the Brca1 exon 11 isoform (Brca1Delta11/Delta11). (nih.gov)
Additionally, Xpc -deficient mice exhibited a strong increase in mutant frequency in lung compared with Xpa −/− mice, whereas in both models mutant frequency is increased in liver. (aacrjournals.org)
NV556 decreased liver fibrosis in two animal models of Non-alcoholic steatohepatitis (NASH), STAM and methionine-choline-deficient (MCD) mice. (lu.se)

Subchronic exposure to ellagic acid impairs cytotoxic T-cell function and suppresses humoral immunity in mice. (cdc.gov)

In testis extracts from mice lacking Cdk2, there are changes in expression of PMS2, MSH2, and HEI10, which may be reflective of the loss of CDK2 phosphorylation. (lu.se)

1994. Investigations of the genotoxicity and cell proliferative activity of dichlorvos in mouse forestomach. (cdc.gov)

Many investigators once believed that TSE agents infected mainly, if not exclusively, cells of neuronal and lymphoid lineages. (cdc.gov)

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia. (nih.gov)
For the functional screening, Bcl2 - and Myc- expressing primary B cells were infected with a retroviral cDNA library. (haematologica.org)
Inserted cDNA of transformed cells in culture were then identified. (haematologica.org)

as they undergo cell division. (lu.se)

Several pathways involved in cell cycle and DNA repair were affected upon NV651 treatment. (lu.se)

Simultaneously, SC precursors (SCP) derive from the neural crest cells and migrate from the neural tube around embryonic day E10.5 to contact axons and differentiate into immature SC (iSC) around E15/E16 ( Woodhoo and Sommer, 2008 ). (elifesciences.org)

Using mouse genetic models, the authors show that Schwann cells regulate vascularization of the sciatic nerve and are required for a decrease in vascular density postnatally. (elifesciences.org)

pointed out that additional genetic alterations were involved in lymphoma development in an Emu mouse model ectopically expressing Myc and Bcl2 . (haematologica.org)
Catastrophic genetic damage can occur if cells progress to the next phase of the cell cycle before the previous phase is properly completed. (tocris.com)

We have previously reported a mouse model in which Brca1 exon 11 is eliminated in mammary epithelial cells through Cre-mediated excision. (nih.gov)

Direct evidence linking the INK4A locus to tumorigenesis was provided by the targeted disruption of exon 2 of INK4A in mice. (medscape.com)

Stem cells have enormous potential for therapeutic applications due to their ability to differentiate into diverse cell types. (omicsonline.org)
We have previously reported that embryonic stem (ES) cells on E-cadherin substratum form single-cell scattering morphology. (omicsonline.org)
00:39 police is these are hematopoietic stem cells. (yale.edu)
02:32 what makes us Ella stem cell? (yale.edu)
Normally, adult, tissue-specific stem cells are held in a kind of cellular deep freeze called quiescence to avoid unnecessary cell division in the absence of injury. (stanford.edu)
In a 2014 paper published in Nature , Rodgers and Rando showed in laboratory mice that an injury to the muscle of one leg caused a change in the muscle stem cells of the other leg. (stanford.edu)
These cells entered what the researchers called an "alert" phase of the cell cycle that is distinct from either fully resting or fully active stem cells. (stanford.edu)
The fact that muscle stem cells distant from the injury were alerted indicated that the damaged muscle must release a soluble factor that can travel throughout the body to wake up quiescent stem cells. (stanford.edu)
After 2.5 days, the researchers found that muscle stem cells from the recipient animals were in an alert state and completed their first cell division much more quickly than occurred in animals that had received blood serum from uninjured mice. (stanford.edu)
Clearly, blood from the injured animal contains a factor that alerts the stem cells," said Rando. (stanford.edu)

Anatomy29

Organisms20

Diseases7

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes85

Disciplines and Occupations1

Information Science3

Health Care2

Proliferation19

Apoptosis19

Progression9

Differentiation7

Tumors14

Arrest6

Hematopoietic cells2

Regulation5

Proteins6

Protein19

Genes11

Metastasis1

Biology6

Recombinant3

Inhibition4

Embryonic stem1

Immune7

Signaling3

Antibody3

Induces1

Bone marrow6

Vivo5

Germ4

Transgenic2

Significantly3

Transcription3

Vitro2

Lymphomas3

Viability3

Upregulation2

20192

Mutations2

MYELOMA1

Strains3

Gene5

Receptor4

C57BL1

Checkpoints2

Endothelial cells3

Transformation1

Chemotherapy1

Mitotic catastrophe1

Apoptotic3

Malignant1

Membrane1

Importantly2

Degradation1

Deficient4

Suppresses1

Phosphorylation1

19941

Lineages1

CDNA3

Undergo1

Pathways1

Neural crest1

Regulate1

Genetic2

Epithelial1

Disruption1

Stem10