- Alfa-amylase was coupled to cyanogen bromide activated Sephadex G200 at pH8.3 and 7.0. (who.int)
- The serum samples were analyzed for total IgE content by a commercial radioimmunoassay (PRIST), and for specific IgE antibodies by the radioallergosorbent test (RAST) using extracts of swine urine, serum, blood, and dander coupled to cyanogen bromide-activated cellulose beads. (cdc.gov)
- Cyanogen chloride (CK) is a highly volatile and toxic chemical asphyxiant that interferes with the body's ability to use oxygen. (cdc.gov)
- Exposure to cyanogen chloride (CK) can be rapidly fatal. (cdc.gov)
- Cyanogen chloride (CK) has strong irritant and choking effects. (cdc.gov)
- Cyanogen chloride (CK) is a chemical warfare agent (military designation CK). (cdc.gov)
- Indoor Air: Cyanogen chloride (CK) can be released into indoor air as a liquid spray (aerosol) or as a gas. (cdc.gov)
- Water: Cyanogen chloride (CK) can be used to contaminate water. (cdc.gov)
- Agricultural: If cyanogen chloride (CK) is released into the air as a liquid spray (aerosol), it has the potential to contaminate agricultural products. (cdc.gov)
- If cyanogen chloride (CK) is released as a gas, it is highly unlikely to contaminate agricultural products. (cdc.gov)
- Cyanogen chloride (CK) can affect the body by inhalation, ingestion, skin contact, or eye contact. (cdc.gov)
- Cyanogen chloride (CNCl or CK) is heavier and will sink to low-lying areas and increase the risk of exposure. (medscape.com)
- the other is cyanogen chloride (NATO designation CK). (medscape.com)
- The introduction of cyanogen chloride by the French in 1916 made available a compound that, being both more toxic and less volatile, was a more effective chemical agent. (medscape.com)
- Plate XXVII (Fig. 1): Lungs of dog, surviving 5 hours, gassed with a high concentration of cyanogen chloride. (sciencehistory.org)
- Cyanogen (chloride and bromide) poisoning. (sciencehistory.org)
- Cyanogen bromide is the inorganic compound with the formula (CN)Br or BrCN. (wikipedia.org)
- Like some other cyanogen compounds, cyanogen bromide undergoes an exothermic trimerisation to cyanuric bromide ((BrCN)3). (wikipedia.org)
- The varied speciality and pharmaceutical companies that we deal in consists of Cyanogen Bromide Cyclopropyl Isothiocyanate, Malonate Derivatives, Methyl 4-Chlorobutyrate, Thiophosgene and many more. (moltuslab.net)
- Methyl bromide poisoning primarily occurs after inhalational exposure, but concurrent dermal exposure might also occur. (cdc.gov)
- Methyl bromide is an ocular, dermal, and mucous membrane irritant. (cdc.gov)
- Detection of bromide below toxic levels does not rule out methyl bromide poisoning. (cdc.gov)
- Detection of methyl bromide in environmental samples, as determined by NIOSH. (cdc.gov)
- A clinically compatible case in which a high index of suspicion (credible threat or patient history regarding location and time) exists for methyl bromide exposure, or an epidemiologic link exists between this case and a laboratory-confirmed case. (cdc.gov)
- Health effects associated with sulfuryl fluoride and methyl bromide exposure among structural fumigation workers. (cdc.gov)
- Methyl bromide intoxication during grain store fumigation. (cdc.gov)
- Death and injury caused by methyl bromide-an insecticide fumigant. (cdc.gov)
- For the final step, several affinity gels were tested and the 1 containing cycloserine coupled to CNBr [cyanogen bromide]-activated Sepharose-4B was selected. (eurekamag.com)
- HCN + HOBr}}} The main uses of cyanogen bromide are to immobilize proteins, fragment proteins by cleaving peptide bonds, and synthesize cyanamides and other molecules. (wikipedia.org)
- Further proteolysis of the cyanogen bromide-generated fragment containing cysteine 5 through cysteine 8, combined with mass spectrometry and Edman degradation, showed that disulfide bonds link cysteine 5 (aa 386) to cysteine 8 (aa 442) and cysteine 6 (aa 390) to cysteine 7 (aa 419). (nih.gov)
- If the sulfur in cysteine attacked cyanogen bromide, the bromide ion would deprotonate the cyanide adduct, leaving the sulfur uncharged and the beta carbon of the cysteine not electrophilic. (wikipedia.org)
- In addition, a number of cyanide-containing compounds, known as cyanogens, may release cyanide during metabolism. (medscape.com)
- Cyanogen bromide is often used to immobilize proteins by coupling them to reagents such as agarose for affinity chromatography. (wikipedia.org)
- Because of its simplicity and mild pH conditions, cyanogen bromide activation is the most common method for preparing affinity gels. (wikipedia.org)
- This vaccine was prepared from the C. parvum oocysts antigen using immune affinity chromatography with cyanogen bromide-activated Sepharose-4B beads. (bvsalud.org)
- Order of cyanogen bromide fragments in the polypeptide chain of human plasma albumin. (cas.cz)
- The carbon atom in cyanogen bromide is bonded to bromine by a single bond and to nitrogen by a triple bond (i.e. (wikipedia.org)
- The electron density in cyanogen bromide is shifted away from the carbon atom, making it unusually electrophilic, and towards the more electronegative bromine and nitrogen. (wikipedia.org)
- Also, cyanogen bromide activation involves the attachment of a ligand to agarose by an isourea bond, which is positively charged at neutral pH and thus unstable. (wikipedia.org)
- however, detection of elevated bromide levels in serum (reference level: 50-100 mg/L) might indicate that an exposure has occurred. (cdc.gov)
- To determine the disulfide bond arrangement of the eight cysteines of gC1(457t),the protein was cleaved with cyanogen bromide. (nih.gov)
- The disadvantages of this approach include the toxicity of cyanogen bromide and its sensitivity to oxidation. (wikipedia.org)
- ATTYGALLE, AB, Studies on immobilization of alfa- amylase to cyanogen bromide activated sephadex g 200, University of Sri Lanka (Colombo Campus) UC(MED), 1977: 50p. (who.int)
- The volume of the cyanogen bromide activated gel, at this pH decreased by about 50 percent, compared to that of the unactivated Sephadex G200. (who.int)
- Chemical cleavage with hydroxylamine and cyanogen bromide also revealed a similar pattern for liganded and unliganded AhR. (nih.gov)
- The polypeptide chain of the highly purified nuclease was subjected to cleavage at its four methionyl bonds with cyanogen bromide. (nih.gov)
- Sequence data were obtained by the manual and solid-phase sequencing of peptides derived from enzymatic digestions with trypsin, chymotrypsin, pepsin, and Staphylococcus aureus protease as well as by chemical cleavage with cyanogen bromide. (elsevierpure.com)
- Cyanogen bromide is often used to immobilize proteins by coupling them to reagents such as agarose for affinity chromatography. (wikipedia.org)
- Cyanogen bromide is also often used because it reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. (wikipedia.org)
- Also, cyanogen bromide activation involves the attachment of a ligand to agarose by an isourea bond, which is positively charged at neutral pH and thus unstable. (wikipedia.org)
- We offer a variety of amino acids coupled through the amino group to cyanogens bromide activated 4% beaded agarose. (sigmaaldrich.com)
- Cyanogen bromide-activated agarose matrices are used for preparation of resins for affinity chromatography. (actascientific.com)
- Cyanogen bromide in base reacts with hydroxyl groups on agarose to form cyanate esters or imidocarbonates (Figure 1). (actascientific.com)
- If the sulfur in cysteine attacked cyanogen bromide, the bromide ion would deprotonate the cyanide adduct, leaving the sulfur uncharged and the beta carbon of the cysteine not electrophilic. (wikipedia.org)
- Isolation and alignment of the five cyanogen bromide fragments and the amino acid sequences of four of the fragments. (nih.gov)
- Cyanogen bromide digestion of the nuclease yielded five fragments, designated A, B, C, D, and E. These fragments have been purified and analyzed for amino acid composition. (nih.gov)
- The chemical cyanogen bromide is commonly used to break polypeptides into smaller fragments. (themasterchemistry.com)
- Brenner, Sydney, "Immunology Cyanalation and Cyanogen Bromide Digestion (6 of 8) ," CSHL Archives Repository , Reference SB/6/2/139, accessed December 10, 2023, https://libgallery.cshl.edu/items/show/75424. (cshl.edu)
- Historically, cyanogen bromide activated matrices have been very popular. (sigmaaldrich.com)
- The synthesis of norlysergamides (26-33) by reaction of lysergamides (4) (LSD) and (10-16) with cyanogen bromide to (18-15) and subsequent hydrolysis with HCl-dioxane is reported. (erowid.org)
- Nor-LSD (26) was converted to its N6-allyl, -ethyl and -propyl - derivatives by reaction with allyl bromide, EtI or PrBr in the presence of NaH. (erowid.org)
- [6] It is preferable over benzyl bromide for the preparation of this reagent, since the reaction of the bromide with magnesium tends to form the Wurtz coupling product 1,2-diphenylethane . (wikipedia.org)
- The disadvantages of this approach include the toxicity of cyanogen bromide and its sensitivity to oxidation. (wikipedia.org)
- Cyanogen bromide is indicated as well, but with a different sensitivity. (whiteoakenv.com)
- Labeling was effected with cyanogen bromide activation and aminoalkylation for specific detection of endogeneous sugar receptors, especially lectins. (uni-regensburg.de)
- Cyanogen bromide-activated sepharose is a readily available commercial product. (actascientific.com)
- The serum samples were analyzed for total IgE content by a commercial radioimmunoassay (PRIST), and for specific IgE antibodies by the radioallergosorbent test (RAST) using extracts of swine urine, serum, blood, and dander coupled to cyanogen bromide-activated cellulose beads. (cdc.gov)
- The carbon atom in cyanogen bromide is bonded to bromine by a single bond and to nitrogen by a triple bond (i.e. (wikipedia.org)
- These interactions are inhibited by denatured, single collagen chains or a subset of their cyanogen bromide peptides in a dose-dependent manner. (nih.gov)