• The name cyanobacteria refers to their color (from Ancient Greek κύανος (kúanos) 'blue'), which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. (wikipedia.org)
  • The tiniest of creatures: little ocean-dwelling cyanobacteria (sometimes known as blue-green algae) that had developed a new super-power never before seen on planet Earth: photosynthesis. (universetoday.com)
  • Bryant explained that, in 1967, two groups of researchers concluded that an important energy-making cycle was incomplete in cyanobacteria - photosynthetic bacteria formerly known as blue-green algae. (world.edu)
  • Within the cyanobacteria, only a few lineages colonized the open ocean: Crocosphaera and relatives, cyanobacterium UCYN-A, Trichodesmium, as well as Prochlorococcus and Synechococcus. (wikipedia.org)
  • Here, we characterize the in vitro and in vivo properties of the SeAgo protein from the mesophilic cyanobacterium Synechococcus elongatus . (nih.gov)
  • The circadian clock of the cyanobacterium Synechococcus elongatus is a discrete nanomachine comprising three proteins - KaiA, KaiB, and KaiC - which interact progressively to set up the timekeeping mechanism, and two kinases whose activities are altered by engaging the Kai oscillator. (ucsd.edu)
  • Transcriptomic and Phenomic Investigations Reveal Elements in Biofilm Repression and Formation in the Cyanobacterium Synechococcus elongatus PCC 7942. (ucsd.edu)
  • A microcin processing peptidase-like protein of the cyanobacterium Synechococcus elongatus is essential for secretion of biofilm-promoting proteins. (ucsd.edu)
  • The circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 drives oscillations in global mRNA transcript abundances with 24 h periodicity under continuous light conditions. (harvard.edu)
  • Brian Pfleger, University of Wisconsin-Madison, Madison, WI, USA, and colleagues built a novel inducible small RNA (sRNA) system to control gene expression in the fast-growing, cyanobacterium Synechococcus sp. (chemistryviews.org)
  • Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. (chemistryviews.org)
  • To re-test the 1967 hypothesis, the team performed new biochemical and genetic analyses on a cyanobacterium called Synechococcus sp. (world.edu)
  • The scientists discovered that Synechococcus indeed had genes that coded for one important alternative enzyme, succinic semialdehyde dehydrogenase, and that adjacent to the gene for this enzyme was a misidentified gene that subsequently was shown to encode a novel enzyme, 2-oxo-glutarate decarboxylase. (world.edu)
  • Cyanobacteria (/saɪˌænoʊbækˈtɪəri.ə/), also called Cyanobacteriota or Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. (wikipedia.org)
  • Cyanobacteria are a group of photosynthetic bacteria evolutionarily optimized for environmental conditions of low oxygen. (wikipedia.org)
  • By examining thousands of species of modern bacteria, they were able to find at least 34 examples of horizontal gene transfer in cyanobacteria's history. (universetoday.com)
  • The results show that all cyanobacteria living today can be traced to a common ancestor from 2.9 billion years ago and that the ancestors of cyanobacteria branched off from other bacteria around 3.4 billion years ago. (universetoday.com)
  • They concluded that cyanobacteria lacked the ability to make one enzyme, called 2-oxoglutarate dehydrogenase, and that this missing enzyme rendered the bacteria unable to produce a compound - called succinyl-coenzyme A - for the next step in the TCA cycle. (world.edu)
  • Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
  • Cyanobacteria are the first organisms known to have produced oxygen. (wikipedia.org)
  • The cyanobacteria Synechocystis and Cyanothece are important model organisms with potential applications in biotechnology for bioethanol production, food colorings, as a source of human and animal food, dietary supplements and raw materials. (wikipedia.org)
  • Cyanobacteria are a group of photosynthetic prokaryotes that have a diverse morphology, minimal nutritional requirements and metabolic plasticity that has made them attractive organisms to use in biotechnological applications. (1library.org)
  • Cells of diverse organisms, from cyanobacteria to humans, use a circadian (24 h) clock to control physiological events and gene expression. (ucsd.edu)
  • Because cyanobacteria grow photosynthetically using water and CO 2 and are easy to manipulate genetically, they are attractive organisms for the production of molecules that have industrial applications. (ucsd.edu)
  • However shotgun analysis of multiple uncultured genomes from a variety of sources have established that cyanobacteria are part of a much larger group of organisms, and that the photosynthetic branch now called the Oxyphotobacteria appear to have evolved from a common ancestor that did not possess the photosyntheitc machinery of current cyanobacteria. (dhushara.com)
  • Cyanobacteria are autotrophic prokaryotes , i.e., organisms that produce complex organic compounds themselves. (chemistryviews.org)
  • Kacar and her team compared rubisco gene sequences from modern organisms to infer what the sequence must have been in their common ancestor . (newscientist.com)
  • The genetic components of a approximately 24-hour clock are found in all organisms so far studied, from cyanobacteria to human beings, with few exceptions. (cdc.gov)
  • Besides the morphological diversity, cyanobacteria are versatile microorganisms with simple growth requirements that are able to produce several secondary metabolites of commercial interest [3-5]. (1library.org)
  • Cyanobacteria are photosynthetic microorganisms that can utilise white, far-red or UV light. (exeter.ac.uk)
  • These microorganisms have an ETR1 -like gene, but no CTR1 gene, so their ethylene response system would have to be different from that of land plants. (scientificamerican.com)
  • However, the synthetic biology tools to predictably control gene expression in cyanobacteria are far behind those developed for other microorganisms. (chemistryviews.org)
  • Phylogenetic affiliations of mesopelagic acantharia and acantharian-like environmental 18S rRNA genes off the southern California coast. (mbl.edu)
  • b) A maximum likelihood tree based on 16S rRNA genes from the class Melainabacteria obtained by Di Rienzi et al. (dhushara.com)
  • In the genome are 6312 protein-encoding genes, two sets of rRNA genes, and 42 tRNA genes representing 41 tRNA species. (kenyon.edu)
  • PCC 7120 possesses an intact but partially degenerated gas vesicle gene cluster, indicating that the natural isolate might be able to produce gas vesicles under some given environmental stimuli for better floatation. (biomedcentral.com)
  • Cyanobacteria are a multifaceted group of photosynthetic prokaryotes, with a large morphological diversity including unicellular, colonial and filamentous forms. (1library.org)
  • Blue light induces major changes in the gene expression profile of the cyanobacterium Synechocystis sp. (wetsus.nl)
  • The six genes exhibiting less variation in each organism were evaluated in terms of their expression stability using geNorm, NormFinder and BestKeeper. (1library.org)
  • The comprehensive evaluation of gene expression patterns is an important part of this characterization and, at present, the quantitative real-time polymerase chain reaction (RT-qPCR) is considered the gold standard for measurement of transcript abundance [6]. (1library.org)
  • Our research focuses on understanding the key events that enable the clock to tell time, become set to local time, and regulate global patterns of gene expression and metabolism. (ucsd.edu)
  • Real-time PCR assays showed that the gvpA gene is constitutively transcribed in vivo, and its expression level is upregulated at low light intensity and/or high growth temperature. (biomedcentral.com)
  • Functional expression of this intact gvp gene cluster enables the recombinant Escherichia coli to gain the capability of floatation in the liquid medium, thanks to the assembly of irregular gas vesicles. (biomedcentral.com)
  • This study examines the gene expression of 33 fatty acid-related genes when the cyanobacterium Chlorogloeopsis fritschii PCC 6912 is grown under far-red light compared to white light using RNA-seq data from the NCBI database. (exeter.ac.uk)
  • Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. (frontiersin.org)
  • These findings imply that similar to the situation in mitochondria and plastids, also in P. chromatophora nuclear factors evolved that control metabolite exchange and gene expression in the chromatophore. (frontiersin.org)
  • Apparently these chromatophore-targeted proteins evolved convergently to plastid-targeted expression regulators and are likely involved in gene expression control in the chromatophore. (frontiersin.org)
  • Chromatin plays a vital role in the implementation of gene expression programs. (harvard.edu)
  • Central to this investigation is an understanding of what gene products are required and expressed during a natural infection and how this expression changes over time (from initial colonization to causation of disease and spread of the pathogen to new hosts) and space (in different cells or tissues within the host). (cdc.gov)
  • Although analyses that give information on the expression of a few genes provide insight and have been responsible for a large proportion of the bacterial pathogenesis literature currently available, our ultimate goal is to understand expression changes across the whole genome. (cdc.gov)
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
  • Two of these methods, signature-tagged mutagenesis and in vivo expression technology, do not directly measure gene expression and do not allow true genomic-scale analysis, but they have been devised to identify genes necessary for pathogens during real infections. (cdc.gov)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • J. Glass, A. Poret-Peterson, F. Wolfe-Simon and A. Anbar, "Molybdenum Limitation Induces Expression of the Molybdate-Binding Protein Mop in a Freshwater Nitrogen-Fixing Cyanobacterium," Advances in Microbiology , Vol. 3 No. 6A, 2013, pp. 9-15. (scirp.org)
  • The researchers genetically modified tobacco plants to carry a gene expression system they've called Highlighter. (cosmosmagazine.com)
  • The circadian rhythm is a pattern of approximately 24 hours with characteristic physiologic and metabolic cycles of, among many other attributes, body temperature, hormone secretion, sleep/wake cycle, alertness, and gene expression (Takahashi et al. (cdc.gov)
  • Central to the regulation of autophagy is the transcription factor EB (TFEB), which coordinates the expression of lysosomal biogenesis and autophagy genes. (bvsalud.org)
  • Cyanobacteria are a very large and diverse phylum of photoautotrophic prokaryotes. (wikipedia.org)
  • Fig 3: Phylogenetic trees of the phylum Cyanobacteria. (dhushara.com)
  • a) A maximum likelihood tree of the phylum Cyanobacteria based on the concatenated alignment of 83 phylogenetically conserved proteins. (dhushara.com)
  • Together the Cyanobacteria and Proteobacteria comprised >90% of each bloom bacterial community sample, although the dominant phylum varied between systems. (tennessee.edu)
  • Bacterial community structures, based on 16S rRNA gene amplicon sequencing, were different between the smaller and larger granules. (wattsupwiththat.com)
  • none]] ==Cell structure and metabolism== Microcystis aeruginosa is a unicellular, planktonic freshwater cyanobacterium. (kenyon.edu)
  • To exploit this biotechnological potential and use cyanobacteria as cell factories it is necessary to understand their physiology and metabolism at a systems level. (1library.org)
  • This synthetic biology tool can be used to study the metabolism and generate new industrial strains of cyanobacteria. (chemistryviews.org)
  • In Taihu, the genetic elements associated with the assimilation and metabolism of nitrogen were predominantly associated with Proteobacteria, while these functions in the North American lakes were primarily contributed to by the Cyanobacteria . (tennessee.edu)
  • Unlike heterotrophic prokaryotes, cyanobacteria have internal membranes. (wikipedia.org)
  • Cyanobacteria are globally widespread photosynthetic prokaryotes and are major contributors to global biogeochemical cycles. (wikipedia.org)
  • A total of about 700 genes out of the 3661 predicted protein-coding genes correspond to integral membrane proteins. (harvard.edu)
  • From these lineages, nitrogen-fixing cyanobacteria are particularly important because they exert a control on primary productivity and the export of organic carbon to the deep ocean, by converting nitrogen gas into ammonium, which is later used to make amino acids and proteins. (wikipedia.org)
  • Some cyanobacteria form harmful algal blooms causing the disruption of aquatic ecosystem services and intoxication of wildlife and humans by the production of powerful toxins (cyanotoxins) such as microcystins, saxitoxin, and cylindrospermopsin. (wikipedia.org)
  • Two systems were developed for functional analysis of the gene clusters by gene disruption or replacement. (microbiologyresearch.org)
  • pHZ1358 was shown to be generally useful for generating mutant strains by gene disruption and replacement in NS3226 as well as in several other Streptomyces strains. (microbiologyresearch.org)
  • It was shown in Cyanobacteria that disruption leads to the loss of high-affinity urea transport activity. (nih.gov)
  • Cyanobacteria produce a range of toxins known as cyanotoxins that can pose a danger to humans and animals. (wikipedia.org)
  • Toxins produced by bloom-associated cyanobacteria can have drastic impacts on the ecosystem and surrounding communities, and bloom biomass can disrupt aquatic food webs and act as a driver for hypoxia. (tennessee.edu)
  • Cyanobacteria may adopt different strategies to reduce the possibility of being consumed by their upper trophic level of food web, such as morphology and intracellular toxins. (kenyon.edu)
  • 12 ] reviewed case reports and anecdotal references that describe illnesses associated with exposure to cyanobacteria and related toxins during recreational activities in freshwater environments. (cdc.gov)
  • We studied the effect of molybdenum (Mo) concentration on transcription and translation of a putative Mo-storage protein (Mop) in the freshwater heterocystous cyanobacterium, Nostoc sp. (scirp.org)
  • Cloning and characterisation of a hyp gene cluster in the filamentous cyanobacterium Nostoc sp. (wikigenes.org)
  • hupS and hupL constitute a transcription unit in the cyanobacterium Nostoc sp. (wikigenes.org)
  • The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
  • This pattern was particularly noticeable in the genomic contribution of nitrogen assimilation genes. (tennessee.edu)
  • This proposal focuses on rare cyanobacteria, with the genomic data used for synthetic biology approaches of drug discovery under condition of climate change. (doe.gov)
  • The circadian clock and darkness control natural competence in cyanobacteria. (ucsd.edu)
  • 2007). Meanwhile, new research in the field of genetics has shown that there may be other relevant mechanisms, besides melatonin production, which are also controlled by the genes involved in maintaining the circadian rhythm (Stevens et al. (cdc.gov)
  • Finally, we show that specific gene knockouts in i SO595 exhibit dynamic anomalies compatible with experimental observations, further demonstrating the value of this model as a tool to probe the metabolic dynamic of Prochlorococcus . (biorxiv.org)
  • PCC 7002, scouring its genome for genes that might be responsible for making alternative energy-cycle enzymes. (world.edu)
  • Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria. (wikipedia.org)
  • The cercozoan amoeba Paulinella chromatophora contains photosynthetic organelles-termed chromatophores-that evolved from a cyanobacterium ∼100 million years ago, independently from plastids in plants and algae. (frontiersin.org)
  • Green algae, generally thought to lie between cyanobacteria and land plants in the evolutionary tree, do not perceive ethylene, so how ethylene responses jumped from cyanobacteria directly into land plants also interests researchers. (scientificamerican.com)
  • Chloroplasts originated from an endosymbiotic event where a Gram-negative cyanobacterium was engulfed by an ancestral eukaryotic cell. (uwaterloo.ca)
  • Computer algorithms are used to search for strings of genetic code to identify genes. (world.edu)
  • Furthermore, the identification of a pfa gene cluster may highlight a secondary path of polyunsaturated fatty acids within heterocyst cyanobacteria. (exeter.ac.uk)
  • Also, notably, particularly large amounts of filamentous cyanobacteria were found from same sampling area. (wattsupwiththat.com)
  • Ribosomal RNA analysis confirmed that filamentous cyanobacteria Phormidesmis priestleyi , also found in Antarctic lakes and marshes, is the predominant species contributing to granule formation and growth. (wattsupwiththat.com)
  • The filamentous cyanobacterium Phormidesmis priestleyi was the dominant bacterial species in larger granules. (wattsupwiththat.com)
  • 2006). Genes involved in the maturation of hydrogenase(s) in the nonheterocystous cyanobacterium Lyngbya majuscula CCAP 1446/4 . (up.pt)
  • aeruginosa (species) ==Species== Mycrocystis aeruginosa ==Description and significance== Mycrocystis aeruginosa is a common unicellular colonial cyanobacteria found in fresh water environments. (kenyon.edu)
  • Gene regulation in fatty acid pathways in cyanobacteria exposed to far-red light. (exeter.ac.uk)
  • Additionally, the team showed that the native RNA binding Hfq protein - critical for regulation of sRNA systems - was not involved in the function of the adapted sRNA system.The Hfq interruption suppresses the natural competency of the cyanobacterium strain. (chemistryviews.org)
  • Based on the three algorithms, we provide a list of genes for cyanobacterial RT-qPCR data normalization. (1library.org)
  • Bryant also said that his team found that the genes coding for the two enzymes are present in all cyanobacterial genomes except those of a few marine species. (world.edu)
  • Now that we understand better how cyanobacteria make energy, it might be possible to genetically engineer a cyanobacterial strain to synthesize 1,4-butanediol - an organic compound that is the precursor for making not just biofuels but also plastics,\" Bryant said. (world.edu)
  • Since then, cyanobacteria have been essential players in the Earth's ecosystems. (wikipedia.org)
  • During this time, cyanobacteria experienced a period of enormous growth worldwide, and it may have been this expansion that littered Earth's atmosphere with molecular oxygen. (universetoday.com)
  • A resurrected gene, brought back from the dead in the lab, is allowing molecular biologists to travel billions of years into the past to study one of the most significant transitions in Earth's history. (newscientist.com)
  • Contrasting this date is an earlier date for the major expansion of genetic diversity, which shows an explosion of the key genes occupying central metabolic pathways beginning around 3.3 billion years ago, including electron transport components. (dhushara.com)
  • Fig 2: Tree diagram of the birth, transfer, duplication and loss of key genes in the redox and electron transport pathways, in a founding burst of gene evolution between 3.3 and 2.7 billion years ago (David and Alm 2010). (dhushara.com)
  • RIViT-seq technology could speed up associating transcription factors with their target genes. (doe.gov)
  • In addition to a suite of genetic tools that enable gene inactivation and overexpression and use of reporter genes, we employ a dense bar-coded transposon library that enables the fitness of thousands of mutants to be assessed in a population under specific test conditions. (ucsd.edu)
  • Through development of genetic tools, support of a metabolic model, and characterization of different growth modes such as biofilm formation, we seek to improve the prospects for cyanobacteria as industrial production platforms. (ucsd.edu)
  • The additional information generated by whole genome studies goes far beyond that derived by characterizing in isolation more genes and gene products, because analysis of the whole genome allows complete regulatory networks to be identified and characterized. (cdc.gov)
  • The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. (lu.se)
  • He explained that, after researchers in the 1960s concluded that cyanobacteria had an incomplete TCA cycle, that false assumption was compounded by later researchers who used modern genomics-research methods to confirm it. (world.edu)
  • Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. (lu.se)
  • Using a particle gun, wild type C. reinhardtii cells have been transformed with a plasmid carrying the psaC gene disrupted by an aadA gene cassette designed to express spectinomycin/streptomycin resistance in the chloroplast. (unige.ch)
  • This occurs when genes jump from one species to another, after being eaten, for example (as opposed to vertical gene transfer, which occurs from parent to child). (universetoday.com)
  • With this method, its possible to date certain species, because the species which received the gene is always younger than the species that it originated in. (universetoday.com)
  • Fournier's research showed that just before the Great Oxidation, there was a sudden dramatic period of diversification in cyanobacteria species. (universetoday.com)
  • Putative acquisitions of photosystem and flagella genes are indicated by colored arrows. (dhushara.com)
  • The chloroplast gene psaC encoding the iron sulfur protein of photosystem I (PSI) from the green alga Chlamydomonas reinhardtii has been cloned and characterized. (unige.ch)
  • To date, a series of cyanobacteria have been found to produce GVs, such as A. flos-aquae , Calothrix sp. (biomedcentral.com)
  • The transcriptomic analysis, encompassing a bioinformatic pipeline to process and quantify transcripts, found that only two genes of the 33 examined genes were differentially expressed. (exeter.ac.uk)
  • This protein is found in a conserved five-gene transport operon typically found adjacent to urease genes. (nih.gov)
  • During studies 44 years ago, researchers concluded that cyanobacteria were missing an essential enzyme of the metabolic pathway that is found in most other life forms,\" Bryant explained. (world.edu)
  • For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR) is the standard technique. (1library.org)
  • While authentic transcripts of the psaC gene are no longer detected, aadA gives rise to a few transcripts in the transformants. (unige.ch)
  • Cyanobacteria are ubiquitous in marine environments and play important roles as primary producers. (wikipedia.org)
  • So if researchers don\'t use biochemical methods to validate computer-identified gene functions, they run the risk of making premature and often incorrect conclusions about what\'s there and what\'s not there. (world.edu)
  • A region in cluster A (∼133 kb) seemed to be involved in nanchangmycin production because replacement of several DNA fragments in this region by an apramycin resistance gene [ aac3(IV) ] gave rise to nanchangmycin non-producing mutants. (microbiologyresearch.org)
  • cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme. (microbiologyresearch.org)
  • The deduced amino acid sequence is highly related to that of higher plants and cyanobacteria. (unige.ch)
  • Forty-five percent of the protein-encoding sequences showed sequence similarity to genes of known function, 32% were similar to hypothetical genes with a hypothetical function, and the remaining 23% had no apparent similarity and code for a unique function [2]. (kenyon.edu)
  • Highlighter is an important step forward in the development of optogenetics tools in plants and its high-resolution gene control could be applied to study a large range of fundamental plant biology questions," says Jones. (cosmosmagazine.com)
  • Towards this goal we have selected and analyzed twelve candidate reference genes from three morphologically distinct cyanobacteria grown under routinely used laboratory conditions. (1library.org)
  • An unexpected role for leucyl aminopeptidase in UV tolerance revealed by a genome-wide fitness assessment in a model cyanobacterium. (ucsd.edu)
  • His research interests include investigating the biochemical and molecular changes in microalgae and cyanobacteria exposed to different environmental conditions. (exeter.ac.uk)
  • The team then compared those findings with molecular clock dating models, giving them the best estimate yet for the origin of modern cyanobacteria. (universetoday.com)
  • As Fournier explains, "this work shows that molecular clocks incorporating horizontal gene transfers promise to reliably provide the ages of groups across the entire tree of life, even for ancient microbes that have left no fossil record … something that was previously impossible. (universetoday.com)