• MPF was measured in vivo using the fast single-point method in three animal groups (control, cuprizone-induced demyelination, and remyelination after cuprizone withdrawal) and compared to quantitative immunohistochemistry for myelin basic protein (MBP), myelinating oligodendrocytes (CNP-positive cells), and oligodendrocyte precursor cells (OPC, NG2-positive cells) in the corpus callosum, caudate putamen, hippocampus, and cortex. (tsu.ru)
  • BACKGROUND: Cuprizone leads to demyelination of the corpus callosum (CC) and activates progenitor cells in the adjacent subventricular zone (SVZ), a stem cell niche which contributes to remyelination. (ox.ac.uk)
  • After 6 weeks of demyelination, extent of demyelination, oligodendrocyte density, microglia infiltration, and axonal damage were analyzed in the corpus callosum. (neurology.org)
  • Histological correlation of diffusional kurtosis and white matter modeling metrics in cuprizone-induced corpus callosum demyelination. (musc.edu)
  • By the third week of cuprizone feeding, consistent demyelination can be observed in the corpus callosum, the largest white matter tract in the mouse brain. (msdiscovery.org)
  • In parallel and despite the presence of the toxicant, oligodendrocyte precursors mature and begin partial remyelination of the corpus callosum at the height of demyelination ( Matsushima and Morell, 2001 ). (msdiscovery.org)
  • In the demyelination group, MPF, MBP-stained area, and oligodendrocyte count were significantly reduced, while OPC count was significantly increased as compared to both control and remyelination groups in all anatomic structures (p (tsu.ru)
  • Most importantly, antibody-dependent TREM2 activation on microglia increased density of oligodendrocyte precursors in areas of demyelination, as well as the formation of mature oligodendrocytes thus enhancing remyelination and axonal integrity. (quanterix.com)
  • To test whether Toll-like receptor (TLR) signaling plays a key role for reduced nuclear factor B (NF-κB) activation after laquinimod treatment in the model of cuprizone-induced demyelination, oligodendrocyte apoptosis, inflammation, and axonal damage. (neurology.org)
  • This might be due to the fact that remyelination is a sophistically orchestrated process that calls for the interplay between oligodendrocyte lineage cells, neurons, central nervous system (CNS) resident innate immune cells, and peripheral immune infiltrates and that this process may somewhat differ in humans and rodent models used in research. (encyclopedia.pub)
  • It showed that Olesoxime dose-dependently promoted oligodendrocyte maturation and myelination in several in vitro (oligodendrocyte progenitor cells, oligodendrocyte - neuron co-cultures, organotypic brain slice cultures) and two in vivo models (cuprizone and lysophosphatidyl choline induced demyelination). (rdworldonline.com)
  • These studies revealed that Gpr37−/− mice exhibit dramatically increased loss of myelin in response to cuprizone, yet do not show any increased loss of oligodendrocyte precursor cells or mature oligodendrocytes. (smith.edu)
  • These findings reveal that loss of GPR37 alters oligodendrocyte physiology and increases susceptibility to demyelination, indicating that GPR37 could be a potential drug target for the treatment of demyelinating diseases such as multiple sclerosis. (smith.edu)
  • Cuprizone treatment is the most frequently used among the toxicant-induced MS models, which include lysolecithin and ethidium bromide, and is used to study mechanisms of oligodendrocyte turnover, astrogliosis, and microgliosis ( Blakemore and Franklin, 2008 ). (msdiscovery.org)
  • Investigators inject cuprizone-fed mice with rapamycin, a drug that blocks mTOR and spontaneous remyelination, allowing better quantification of oligodendrocyte turnover. (msdiscovery.org)
  • Unraveling the molecular mechanisms of this potentially predetermined remyelination pattern and identifying oligodendrocyte progenitor populations with repair capacity may be the basis for future disease-modifying therapies in MS. A vital but still poorly understood component of this endogenous replacement mechanism is the prominent astrogliosis and microgliosis at the demyelination sites. (msdiscovery.org)
  • Cuprizone-induced oligodendrocyte loss and demyelination impairs recording performance of chronically implanted neural interfaces. (kozailab.com)
  • Effects of dietary intervention on MRI activity, de- and remyelination in the cuprizone model for demyelination. (uib.no)
  • CONCLUSIONS: This study reveals a uniquely regulated inflammatory response in the SVZ and shows that Gal-3 participates in remyelination in the cuprizone model. (ox.ac.uk)
  • Cuprizone increased the density of CD45+/Iba1+ microglial cells and also increased Gal-3 expression in the CC. Surprisingly, the number of Gal-3+ and CD45+ cells decreased in the SVZ after cuprizone, suggesting inflammation was selectively reduced therein. (ox.ac.uk)
  • Inflammation can regulate SVZ proliferation and indeed the number of phosphohistone H3+ (PHi3+) cells decreased in the SVZ but increased in the CC in both genotypes after cuprizone treatment. (ox.ac.uk)
  • This contrasts with more severe models of demyelination which induce SVZ inflammation and suggests the extent of demyelination affects the SVZ neurogenic response. (ox.ac.uk)
  • MS is a chronic inflammatory demyelinating disease of the CNS causing focal lesions in white and gray matter, 1 , 2 which are pathologically characterized by inflammation, demyelination, microglial activation, astrogliosis, and axonal injury. (neurology.org)
  • However, the precise role of CCR6 has been controversial, in part because no effective monoclonal antibody (mAb) inhibitors against this receptor exist for use in mouse models of inflammation. (jci.org)
  • Two groups have reported that administration of COX-2 inhibitors in experimental autoimmune encephalomyelitis (EAE) diminished the severity and incidence of disease and decreased demyelination and inflammation [11, 12]. (healthandwellnesssource.org)
  • MS is usually a debilitating central nervous system (CNS) autoimmune disease that consists of CNS-directed inflammation demyelination and axonal degeneration. (bio-cavagnou.info)
  • Crude Turmeric Extract Improves the Suppressive Effects of Lactobacillus rhamnosus GG on Allergic Inflammation in a Murine Model of House Dust Mite-Induced Asthma. (shengsci.com)
  • This study aimed to assess the capability of MPF to quantify remyelination using the murine cuprizone-induced reversible demyelination model. (tsu.ru)
  • To investigate the impact of episodes of reversible demyelination on long-term locomotor performance and neuro-axonal integrity, cuprizone-treated animals were monitored using motor skill sequence (MOSS), to observe whether a functional recovery was attained and maintained in the long-term. (uni-goettingen.de)
  • Dietary vitamin D3 supplements reduce demyelination in the cuprizone model. (uib.no)
  • In addition, our group has shown that COX-2 inhibitors reduce demyelination in the TMEV model of MS [8]. (healthandwellnesssource.org)
  • When administered orally in mice, cuprizone, a copper chelator, causes rapid demyelination and gliosis, or rapid proliferation of glia subtypes. (msdiscovery.org)
  • Trem2 Activation On Microglia Promotes Myelin Debris Clearance And Remyelination In A Model Of Multiple Sclerosis. (quanterix.com)
  • Microglia are critical for the clearance of myelin debris in areas of demyelination, a key step to allow remyelination. (quanterix.com)
  • The combined secretion of cytokines by lymphocyte infiltrates, activated M1 microglia, and A1 astrocytes lead to OL death and demyelination [ 4 , 5 ]. (encyclopedia.pub)
  • Clearance of myelin debris by macrophages and microglia following demyelination is a crucial process to allow remyelination. (encyclopedia.pub)
  • We observed that it results in a fatal disease where demyelination of white matter tracts is accompanied by microglia activation and axonal damage. (uni-goettingen.de)
  • RESULTS: Cuprizone caused loss of myelin basic protein (MBP) immunofluorescence in the CC suggesting demyelination. (ox.ac.uk)
  • To study the short-term impact of demyelination, histological analysis was performed in a mouse model where myelinating oligodendrocytes are ablated by the expression of diphtheria toxin receptor in mature oligodendrocytes and systemic diphtheria toxin injection. (uni-goettingen.de)
  • To further investigate whether myelin defects can cause plaque deposition, they induced acute demyelination in 5xFAD mice by feeding them Cuprizone. (biologists.com)
  • Objective To investigate the difference in tissue distribution of ligustrazine hydrochloride and Salvia miltiorrhiza (Danshen) in acute myocardial ischemia (AMI) model rats. (alljournal.net.cn)
  • In the acute cuprizone paradigm, male C57BL/6 mice at 6 to 9 weeks of age are fed a diet of chow mixed with 0.2% cuprizone over the course of 6 weeks. (msdiscovery.org)
  • Cuprizone demyelination induces a unique inflammatory response in the subventricular zone. (ox.ac.uk)
  • Prolonged cuprizone exposure induces chronic demyelination and impairs the capacity of the brain to repair. (msdiscovery.org)
  • Macromolecular proton fraction (MPF) has been established as a quantitative clinically-targeted MRI myelin biomarker based on recent demyelination studies. (tsu.ru)
  • Demyelination on exposure to cuprizone is accompanied by predictable microglial activation and astrogliosis, and, after cuprizone withdrawal, this activation reproducibly diminishes during remyelination. (elsevierpure.com)
  • A salmon based diet protects mice from behavioural changes in the cuprizone model for demyelination. (uib.no)
  • METHODS: We studied the inflammatory response to cuprizone in the SVZ and CC in Gal-3 knockout mice using immunohistochemistry and with the in vitro neurosphere assay. (ox.ac.uk)
  • BrdU+ SVZ cell numbers also decreased in the SVZ after cuprizone, and this effect was significantly greater at 3 weeks in Gal-3 (-/-) mice compared to WT, suggesting Gal-3 normally limits SVZ cell emigration following cuprizone treatment. (ox.ac.uk)
  • Ten-week-old C57BL/6J, TLR4 − / − , and MyD88 − / − mice received 0.25% cuprizone for 6 weeks and were treated daily with 25 mg/kg laquinimod or vehicle. (neurology.org)
  • Vehicle-treated controls from C57BL/6J, TLR4 − / − , and MyD88 − / − mice displayed extensive callosal demyelination as well as microglial activation. (neurology.org)
  • The demyelination score was significantly higher in all untreated mice compared to mice treated with laquinimod. (neurology.org)
  • assessed amyloid deposition in AD mouse models (5xFAD and APP NLGF , respectively) crossed with mice showing minor structural myelin defects driven by the lack of myelin-proteins like CNP (CNP -/- ) or PLP (PLP -/y ). 5XFAD mice harbor five familial AD (FAD) mutations triggering Aβ overproduction, and already exhibit amyloid deposition two months after birth (4). (biologists.com)
  • Although this copper chelator could possibly interfere with plaque formation, Cuprizone-treated mice showed a substantial increase of amyloid aggregates in regions of strong demyelination. (biologists.com)
  • Similarly, induction of experimental autoimmune encephalomyelitis (EAE) - a model for chronic immune-mediated myelin loss - caused the formation of amyloid plaques in the spinal cord of 5xFAD mice. (biologists.com)
  • Therefore, they created a mouse model with sparsely myelinated cortical axons (forebrain shiverer mice, Emx-Cre MBP fl/fl ) and combined them with 5xFAD mice. (biologists.com)
  • Inhibition of LtβR signaling by an LtβR-Ig fusion decoy protein successfully delayed demyelination in wild-type mice. (elsevierpure.com)
  • In the non- T cell mediated cuprizone model of demyelination MSC administration had a positive effect with an overall increase in myelin abundance in the brain of MSC-treated mice compared to controls. (bio-cavagnou.info)
  • Rescue of synaptic and cognitive functions in polysialic acid-deficient mice and dementia models by short polysialic acid fragments. (mhh.de)
  • As loss of MAG has previously been shown to result in increased susceptibility to brain insults, we additionally assessed Gpr37-knockout (Gpr37−/−) vs. wild-type mice in the cuprizone model of demyelination. (smith.edu)
  • and non-T cell mediated settings using the MOG35-55 experimental autoimmune encephalomyelitis (EAE) and cuprizone-mediated demyelination models respectively. (bio-cavagnou.info)
  • MSCs have been shown to improve experimental models of several autoimmune diseases including Type 1 Diabetes systemic lupus erythematosus rheumatoid arthritis and multiple sclerosis (MS) [1-5]. (bio-cavagnou.info)
  • Machine learning based white matter models with permeability: An experimental study in cuprizone treated in-vivo mouse model of axonal demyelination. (movit.paris)
  • In experimental animals, high-dose HD intoxication was found to cause demyelination in PNS and spinal cords and has been used to study the mechanisms of HD neurotoxicity and evaluate the therapeutical potential of various reagents for demyelinating conditions [ 9 ]. (biomedcentral.com)
  • This targeted anti-inflammatory agent improves physical deficits in experimental autoimmune encephalomyelitis (EAE), an animal model of MS. Here, we sought to determine whether tuftsin is also effective in combination with benztropine, an FDA-approved drug that stimulates remyelination, in both EAE and in the cuprizone model of demyelination. (shengsci.com)
  • Treatment with a new TREM2 agonistic antibody promoted the clearance of myelin debris in the cuprizone model of CNS demyelination. (quanterix.com)
  • Demyelination results in large amounts of myelin debris, composed of cholesterol, phospholipids, glycolipids, and myelin-associated proteins [ 17 ]. (encyclopedia.pub)
  • The neurotoxicant-induced, cuprizone model of demyelination is ideally suited for the analysis of inflammatory events. (elsevierpure.com)
  • We show that combining these two agents to promote anti-inflammatory and remyelinating mechanisms alleviates symptoms in EAE and lessens pathological hallmarks in both MS models. (shengsci.com)
  • Importantly, tuftsin is required to transform the inflammatory CNS environment normally present in EAE/MS into one of an anti-inflammatory nature, and benztropine is required in the cuprizone model to improve remyelination. (shengsci.com)
  • We offer a selection of in vitro, ex vivo and in vivo models for testing new and innovative compounds within the challenging research area of neurodegenerative diseases. (innoserlaboratories.com)
  • We offer highly reproducible in vitro models and assay s using human and rodent cell lines and primary mouse cell types , including monocultures and mixed cultures of neuronal and glial cells. (innoserlaboratories.com)
  • Our in vitro assays and models allow you to investigate the effect of your investigational compound on specific neurological pathways. (innoserlaboratories.com)
  • Phagocytosis assay is an excellent, highly reproducible assay to test compound efficacy using pHRODO -labelled amyloid-β (Aβ) fibrils in an in vitro model of Alzheimer's disease using the human microglial cell line (HMC3). (innoserlaboratories.com)
  • Myelin dysfunction drives amyloid-β deposition in models of Alzheimer's disease. (mpg.de)
  • Diffusion MRI detects basal forebrain cholinergic abnormalities in the 3xTg-AD mouse model of Alzheimer's disease. (musc.edu)
  • She was then a postdoctoral fellow at Sanofi-Aventis (France) in Jesus Benavides'team and at the French Alternatives Energy and Atomic Energy Commission(NeuroSpin, MIRCen) in Marc Dhenain's team, where she developed MRI methods for the longitudinal evaluation of amyloid plaque load in a mouse model of Alzheimer's disease. (movit.paris)
  • 6. Androuin A, Abada YS, Ly M, Santin M, Petiet A, Epelbaum S, Bertrand A,Delatour B. Activity-induced MEMRI cannot detect functional brain anomalies in the APPxPS1-Ki mouse model of Alzheimer's disease. (movit.paris)
  • The aim of our study was to investigate the effect of prenatal stress induced by exposure to variable frequency ultrasound (US PS) throughout the gestational period on the adult rat offspring, namely to identify features of behavioral alterations and neurochemical brain parameters that can be associated with certain mental disorders in humans, to determine the possibility of creating a new model of psychopathology. (bvsalud.org)
  • Chronic hexane exposure causes segmental demyelination in the peripheral nerves, and high-dose intoxication may also affect central nervous system. (biomedcentral.com)
  • The animal's gender, age, and exposure time to cuprizone are important determinants of the reproducibility and sequence of de- and remyelination events. (msdiscovery.org)
  • The cuprizone mouse model captures several aspects of MS pathology, bypassing the autoimmune component in other preclinical models. (msdiscovery.org)
  • Drug development for neurodegenerative diseases is currently limited by poor translation of preclinical animal models to clinical application in humans. (innoserlaboratories.com)
  • Although remyelination can be demonstrated by the occurrence of thin myelin using electron microscopy and animal models at different time points after a demyelinating lesion [ 9 ], it has been difficult to conclude whether the thinly myelinated axons in postmortem MS patients are partly demyelinated or incompletely remyelinated axons [ 10 ]. (encyclopedia.pub)
  • Our neurology models are initiated quickly and set up upon agreement with our dedicated neurology study team. (innoserlaboratories.com)
  • Unlike other toxicant-induced models, which are introduced in the brain by stereotaxic microinjections and result in focal demyelination, oral administration of cuprizone produces a global insult. (msdiscovery.org)
  • A novel brain metastases model developed in immunodeficient rats closely mimics the growth of metastatic brain tumours in patients. (uib.no)
  • Our previous study found that BMSCs promoted motor function recovery in rats modeling hexane neurotoxicity. (biomedcentral.com)
  • Results Rat body weight, the relative abundance of intestinal probiotics, and fecal butyric acid and valeric acid contents were decreased in rats in the model group compared with the normal group, while the abdominal withdrawal reflex score, fecal water content, and relative abundance of Blautia spp. (alljournal.net.cn)
  • while rats are resistant to demyelination and therefore do not represent a suitable model to study remyelination events ( Love, 1988 ). (msdiscovery.org)
  • Different rodent strains react in idiosyncratic ways to cuprizone, which is best characterized in the C57BL/6 strain. (msdiscovery.org)
  • Uncoupling the widespread occurrence of anti-NMDAR1 autoantibodies from neuropsychiatric disease in a novel autoimmune model. (mpg.de)
  • Our results confirm that laquinimod prevents demyelination in the cuprizone mouse model for multiple sclerosis via downregulation of NF-κB activation. (neurology.org)
  • Liver kinase B1 depletion from astrocytes worsens disease in a mouse model of multiple sclerosis. (mpg.de)
  • To investigate the impact of impaired myelin on AD pathogenesis, they used various mouse models with genetically or chemically induced myelin dysfunction. (biologists.com)
  • In addition, the cuprizone-induced mouse model of de- and remyelination was used to specifically test the hypothesis that CDP-choline directly increases remyelination. (feedabrain.com)
  • Evidence of altered age-related brain cytoarchitecture in mouse models of down syndrome: a diffusional kurtosis imaging study. (musc.edu)
  • A knock-in mouse model of CACH/VWM, which shows a developmental white matter abnormality, is a promising new tool for the research of this devastating disease. (medlink.com)
  • This page was printed from http://surf.rutgers.edu/2019/11/bile-acid-homeostasis-in-a-cholesterol-7%CE%B1-hydroxylase-and-sterol-27-hydroxylase-double-knockout-mouse-model/ at 12:07 PM Wednesday, September 27, 2023. (rutgers.edu)
  • This study aimed to identify associations between neurogenesis in the neurogenic niches, oligodendrogenesis, and myelination in the murine cuprizone demyelination/remyelination model. (ismrm.org)
  • These studies suggest that COX-2 may have an important role in demyelinating diseases like MS. Studies with COX-2 inhibitors in animal models of MS also support a role for COX-2 as a contributor to disease pathology [11, 12]. (healthandwellnesssource.org)
  • Cortical neuronal densities and cerebral white matter demyelination in multiple sclerosis: a retrospective study. (uib.no)
  • Myelin is also relevant from a clinical perspective, given that demyelination is often observed in several neurological diseases such as multiple sclerosis ( Höftberger & Lassmann, 2018 ). (biorxiv.org)
  • The objective of this study was to determine potential regenerative effects of the substance cytidine-5′-diphospho (CDP)-choline in two different murine animal models of multiple sclerosis. (feedabrain.com)
  • We also have shown that COX-2 is expressed in dying OLs at the onset of demyelination in Theilers Murine Encephalomyelitis Virus (TMEV) model of multiple sclerosis (MS) [9] and in dying OLs in MS lesions [8]. (healthandwellnesssource.org)
  • Demyelination and remyelination of the spinal cord before and after BMSC treatment were examined microscopically. (biomedcentral.com)
  • The development of animal models of mental disorders is an important task, since such models are useful for studying the neurobiological mechanisms of psychopathologies and for trial of new therapeutic drugs. (bvsalud.org)
  • Overall, tuftsin effectively combined with benztropine to significantly improve MS-like pathologies in both models. (shengsci.com)
  • 5. Petiet A , Adanyeguh I, Aigrot MS,Poirion E, Nait-Oumesmar B, Santin M, Stankoff B. Ultrahigh field imaging of myelin disease models: Toward specific markers of myelin integrity? (movit.paris)
  • This study demonstrates enhanced expression of lymphotoxin β receptor (LtβR) during the demyelination phase of this model, and LtβR is found in areas enriched with microglial and astroglial cells. (elsevierpure.com)
  • In addition to its neuroprotective capability, CDP-choline potentiates neurorecovery, which has led to its evaluation as treatment for both stroke and TBI in animal models and in human clinical trials (Cohadon et al. (feedabrain.com)
  • She has been developing biomarkers in various animal models (both rodents and small primates) of brain diseases such as Parkinson's disease and more. (movit.paris)
  • Her research focuses on the validation of translational MRI and MRS biomarkers and on therapeutical evaluations in animal models of brain diseases. (movit.paris)
  • Current and Emerging MR Methods and Outcome in Rodent Models of Parkinson's Disease: A Review. (movit.paris)
  • We demonstrated that this model is therefore well suited for studying demyelination-mediated axonal injury and myelin degradation in the short term. (uni-goettingen.de)
  • According to the "two-step model," the intrathymic generation of CD4⁺ regulatory T (T(reg)) cells segregates into a first, T cell receptor (TCR)-driven phase and a second, cytokine-dependent phase. (shengsci.com)