• Cancer cells and mononuclear cells were isolated, cultured overnight, and analyzed for viability using flow cytometry (FC). (aiche.org)
  • Dermatophatologists observe mononuclear leukocytes in close apposition to keratinocytes (KCs) in graft versus host disease and in other lymphocyte-mediated skin diseases, such as lichen planus, erythema multiforme, and lupus erythematosus. (elsevierpure.com)
  • Since the KCs are Class II histocompatibility antigen (HLA-DR) positive in these diseases (indicating local production of gamma interferon, IFN-γ, by activated T-cells), we sought to determine whether IFN-γ treatment of KCs would influence the ability of allogeneic peripheral blood mononuclear leukocytes (PBMLs) to adhere to cultured KCs in vitro. (elsevierpure.com)
  • Nickoloff, BJ , Lewinsohn, DM & Butcher, EC 1987, ' Enhanced binding of peripheral blood mononuclear leukocytes to γ-interferon-treated cultured keratinocytes ', American Journal of Dermatopathology , vol. 9, no. 5, pp. 413-418. (elsevierpure.com)
  • We analyzed the expression of PD1 and PD-L1 using qRT-PCR in RT4 bladder cancer (BCa) epithelial cells co-cultivated with peripheral blood mononuclear cells (PBMC) after treatment with BCG. (biomedcentral.com)
  • In the present work, we performed an investigation to determine whether the Th17/Treg balance was abnormal in peripheral blood mononuclear cells (PBMCs) of patients with arsenicosis caused by burning coal. (ijbs.com)
  • Genetically modified pECs were co-cultured with human peripheral blood mononuclear cells (PBMCs) for E:T ratio of 10:1 for 2 hours. (iupui.edu)
  • Methods This study used human peripheral blood mononuclear cells from healthy donors and in vitro co-culture of MM cells and BM stromal cells to recapitulate the resistant BM niche. (bmj.com)
  • TruCulture® is a whole blood collection and incubation system that reduces and eliminates variables introduced by traditional culturing of peripheral blood mononuclear cells (PBMCs). (q2labsolutions.com)
  • In the first phase of the project cell lines of specific leukocyte subsets (THP-1/monocyte, IM-9/T lymphocyte, H9/B lymphocyte), PBMCs (peripheral blood mononuclear cells) from healthy donors, respectively will be treated in vitro with hgh to identify candidate genes influenced by hghapplication. (wada-ama.org)
  • To this purpose cell lines of specific leukocyte subsets (THP-1/ monocyte, IM-9/ B- lymphocyte, H9/ T-lymphocyte) and PBMCs (peripheral blood mononuclear cells) from healthy donors, respectively were treated in vitro with hgh to identify candidate hgh-sensitive genes. (wada-ama.org)
  • We therefore investigated the combined effects of physiologic-stress doses of in vitro CT and CS upon the type-1/type-2 cytokine balance and expression of B7 costimulatory molecules of human peripheral blood mononuclear cells (PBMC) as a model to study the immunomodulatory effects of physiologic stress. (tmc.edu)
  • Salicru, Adriano N, "The roles of costimulatory molecules in the cooperative effects of combination epinephrine and dexamethasone on type-1/type-2 cytokine production in tetanus-toxoid specific stimulated human peripheral blood mononuclear cells" (2007). (tmc.edu)
  • NK cells were obtained from peripheral blood (PB) of healthy volunteers. (springer.com)
  • NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
  • 2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
  • A carrier state of mumps virus in human conjunctiva cells. (wikidata.org)
  • A great many noxious agents capable of inducing SCE in tissue culture cells are carcinogenic (see, for example, Wolff, 1977 ). (biologists.com)
  • For metastasis to occur, cancer cells detach from the primary tumor, invade through the basement membrane, and intravasate into the peripheral circulation as circulating tumor cells (CTCs) (2). (aiche.org)
  • 205 or PC-3 cancer cells suspended in buffer or spiked in peripheral human blood were subjected to shear flow in a cone-and-plate viscometer. (aiche.org)
  • Mouse blood was removed via cardiac puncture, and viable and apoptotic cancer cells were analyzed using FC. (aiche.org)
  • Exposure of functionalized ES/TRAIL leukocytes into cancer cell-spiked blood successfully targeted and killed cancer cells under shear flow, compared to controls (Fig. 1B). (aiche.org)
  • Cancer cells lodged within the mouse lung were apoptotic after treatment with ES/TRAIL leukocytes, as determined by multiphoton microscopy. (aiche.org)
  • Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)
  • However, our understanding of how pathogens interact with cellular and humoral host factors in blood is limited, mainly due to technical issues: While it is relatively easy to study the interaction of pathogens with isolated blood cells, or their survival in serum or plasma, such approaches lack the complexity of interactions between different types of immune cells and additional factors, e.g., complement, present in blood. (frontiersin.org)
  • Therefore, we previously established an ex vivo human whole-blood infection model that allowed us to define which immune cells interact with the human fungal pathogen Candida albicans ( 4 ), to identify cross-talk between different components of the host response ( 5 ), and to detect substantial differences between related fungal pathogens ( 6 ). (frontiersin.org)
  • electron-optic demonstration of rotaviruses in human small intestine epithelial cells]. (liverpool.ac.uk)
  • Peripheral blood leukocytes from all normal humans tested have generated trinitrophenyl (TNP)-dependent cytotoxic activity after in vitro sensitization with TNP-modified autologous cells. (aai.org)
  • Effectors lysed not only TNP-modified autologous cells but all TNP-modified human leukocyte targets tested, including those that shared no serologically defined HLA-A, -B, or -C locus determinants with the specific target. (aai.org)
  • Furthermore, unlike ADCC mediated by anti-TNP antibody, the cytotoxic activity was inhibited by TNP-modified autologous cells, but not by TNP-lysine, or TNP-conjugated chicken red blood cells. (aai.org)
  • We conclude that some human cytotoxic T cells raised against TNP-modified autologous cells because they express a receptor for TNP in conjunction with cell surface determinant(s) that are widely shared among humans but not shared with mice. (aai.org)
  • The fibrocyte is one of many populations of bone marrow-derived cells that can be detected in the peripheral blood and is considered to represent a mesenchymal progenitor cell [ 1 ]. (ersjournals.com)
  • In animal models employing surgically implanted wound chambers, it can be shown that ∼16% of cells that accumulate over a number of days are putative blood-derived fibrocytes [ 4 ]. (ersjournals.com)
  • Similarly, CD34+ cells can be identified in human scar tissue, suggesting that these cells may contribute to tissue remodelling and repair [ 5 ]. (ersjournals.com)
  • Recent studies by F rid and co-workers [ 9 - 11 ] in the chronically hypoxic neonatal calf and in rats have shown that a proportion of the adventitial cells of pulmonary hypertensive animals are of a blood-derived fibrocyte phenotype. (ersjournals.com)
  • CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. (bdbiosciences.com)
  • RESULTS: People with and without atopic dermatitis have detectable Der p 2-specific T cells in the peripheral blood, which can proliferate in response to Der p 2 peptides. (ox.ac.uk)
  • Affected individuals are either homozygous for a single pathogenic mutation or compound heterozygous for two different pathogenic variants affecting the function of the pyruvate kinase enzyme in red blood cells and liver. (conduent.com)
  • Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo, in human promyelocytic leukemic cell lines, and ex vivo in human blood neutrophils. (cdc.gov)
  • We analyzed differential gene expression in the peripheral white blood cells (WBC) from 7 female patients diagnosed with occupational benzene poisoning and 7 matched controls. (cdc.gov)
  • Mechanistic analysis revealed that phenol , a major metabolite of benzene, significantly induced the expression of CYP4F3A at both mRNA and protein levels in cultured promyelocytic leukemia cells (HL-60) similarly to all-trans retinoic acid (ATRA). (cdc.gov)
  • Induction of CYP4F3 by phenol was also observed in differentiated HL-60 cells, in pro-erythroid cell line K562, and ex vivo in human neutrophiles. (cdc.gov)
  • The findings demonstrated, for the first time, that benzene and metabolites induce CYP4F3 in human blood cells both in vivo and in vitro. (cdc.gov)
  • HLA-G expression was also examined in cultured thyroid follicular cells (TFCs). (bmj.com)
  • Controversy and debate surround the role of the non-classic human lymphocyte antigen (HLA) class I molecule HLA-G. 1, 2 Initially, the expression of HLA-G was reported to occur only in a limited subset of trophoblast cells at the fetal-maternal interface. (bmj.com)
  • In addition, an approximately 20-kDa soluble form of the K12 protein derived from the N-terminal domain is specifically secreted by cells into the culture medium. (consensus.app)
  • Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. (consensus.app)
  • Replication of dengue and Junin viruses in cultured rabbit and human endothelial cells. (microbiologyresearch.org)
  • MM cells co-cultured with BM stromal cells displayed resistance to reovirus-induced oncolysis and bystander cytokine-killing but remained susceptible to killing by reovirus-activated NK cells and MM-specific cytotoxic T lymphocytes. (bmj.com)
  • The initial step of leukocyte extravasation at sites of inflammation is mediated by selectins leading to the rolling of leukocytes on activated endothelial cells. (uni-giessen.de)
  • To investigate the influence of HMO on leukocyte rolling and adhesion, monocytes, lymphocytes, or neutrophils from human peripheral blood were passed over human endothelial cells in a parallel flow chamber. (uni-giessen.de)
  • Cells were cultured in 6-well plate wells that were pre-coated with anti-CD3 plus mouse IgG2a isotype control (open histogram) or with anti-CD3 plus anti-human TIGIT (clone A15153G) (filled histogram). (biolegend.com)
  • By culturing whole blood, TruCulture retains all leukocyte populations, all Fc receptor bearing cells, and all circulating soluble factors. (q2labsolutions.com)
  • Depending on the culture conditions, cuboidal cells could be produced that contained lamellar bodies, a characteristic marker of type II pneumocytes. (biomedcentral.com)
  • Formation of human composite respiratory mucosa using normal human bronchial cells cocultured with bone marrow mesenchymal stem cells under air-interface conditions allowed for the formation of mucus-producing cells in vitro [ 12 ]. (biomedcentral.com)
  • Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
  • Furthermore, in cultured Fanconi anemia cells, cell cycle arrest in gap 2/mitosis (G2/M) occurs at lower concentrations of clastogens than in normal cells. (medscape.com)
  • The present project aims to introduce microarray technology as a new analytical tool into the field of doping control analysis to gain insight in specific effects of recombinant human growth hormone (hgh) on blood cells (leukocytes). (wada-ama.org)
  • Whole genome cDNA microarrays as well as oligonucleotide microarrays including leukocyte-relevant genes will be used to characterize potential hgh-specific genes by comparing gene expression profiles of treated and untreated cells. (wada-ama.org)
  • The basic conclusion is that MSCs or their closely relevant cousins can reside in or pass via most tissues, and that this kind of cells may be isolated and/or selelck kinase inhibitor cultured by standard methods this kind of as fluorescence activated cell sorting or plastic adherence, and will be shown to possess multipotency. (plasignaling.com)
  • In this research, we hypothesized that Sp110 may be involved in the infection of human promyelocytic HL-60 cells with A. phagocytophilum . (biomedcentral.com)
  • The human Sp110 and A. phagocytophilum msp4 mRNA levels were evaluated by real-time RT-PCR in infected human HL-60 cells sampled at 0, 12, 24, 48, 72 and 96 hours post-infection. (biomedcentral.com)
  • The A. phagocytophilum infection levels were evaluated in HL-60 cells after RNAi by real-time PCR of msp4 and normalizing against human Alu sequences. (biomedcentral.com)
  • These results demonstrated that Sp110 expression is required for A. phagocytophilum infection and multiplication in HL-60 cells, and suggest a previously undescribed mechanism by which A. phagocytophilum modulates Sp110 mRNA levels to facilitate establishment of infection of human HL-60 cells. (biomedcentral.com)
  • In the laboratory, A. phagocytophilum can be propagated in undifferentiated human promyelocytic HL-60 cells. (biomedcentral.com)
  • Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
  • Our products are intended for in-vitro use only for manufacturing and research and are not suitable for human or animal use. (innov-research.com)
  • Here, we describe a novel approach to functionalize human and murine leukocytes with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on their surface, along with E-selectin (ES), to capture and induce cancer cell apoptosis both in vitro in human blood and in vivo in mouse circulation. (aiche.org)
  • We have shown that leukocytes functionalized with ES/TRAIL can capture and induce cancer cell apoptosis in vitro in human blood and in vivo in mouse experiments. (aiche.org)
  • A continued challenge in investigating human immunity is the ability to correlate in vitro data to in vivo responses. (q2labsolutions.com)
  • Development of good in vitro human tissue models would help to bridge the gap in our current knowledge of lung responses and provide a better understanding of lung development, physiology and pathology. (biomedcentral.com)
  • In vitro models of simple one-cell or two-cell culture systems as well as complex multicellular lung analogs that reproduce defined components of specific human lung responses have already been realized. (biomedcentral.com)
  • A benefit of current in vitro lung models is that hypotheses generated from review of data from human or animal disease studies can be tested directly in engineered human tissue models. (biomedcentral.com)
  • Because of these problems, most researchers have utilized in vitro cell culture models to answer basic questions related to human cellular responses in the lung. (biomedcentral.com)
  • One of the earliest models used to study lung development, an in vitro culture of pieces of intact fetal lung, indicated that there were critical differences in the development of submersion-cultured fetal lung cell- scaffold constructs versus air-interface-cultured fetal lung constructs [ 4 ]. (biomedcentral.com)
  • To find additional hgh-candidate genes, suppression subtractive hybridization (SSH) technology will be applied on hgh-treated PBMCs and on those cultured leukocyte subsets which showed a response to hgh before during in vitro studies. (wada-ama.org)
  • Although individual pharmacologic doses of CS and CT can inhibit the expression of T-helper 1 (Th1, type-1 like) and promote the production of T-helper 2 (Th2, type-2 like) cytokines in antigen-specific and mitogen stimulated human leukocyte cultures in vitro, little attention has been focused on the effects of combination physiologic-stress doses of CT and CS that may be more physiologically relevant. (tmc.edu)
  • Furthermore, we developed flow cytometry based assays to detect total β2AR in human CD4+ T-lymphocytes that demonstrated decreased expression of β2AR in mitogen stimulated CD4+ T-lymphocytes in the presence of physiologic stress levels of CS and CT as single in vitro agents, however, when both CS and CT were combined, significantly higher expression of β2AR was observed. (tmc.edu)
  • The authentic characteristic of MSCs their capacity to form fibroblastic colonies in vitro from BM or other tis sues plated onto tissue culture plastic has been extensively exploited, and there's a huge literature on their several phenotypical qualities. (plasignaling.com)
  • Co-localization intersection of 5GKO.HLA-E.HLA-G with stimulated PBMCs was not significantly different than when cultured with media (0.067 vs. 0.059, p=0.57). (iupui.edu)
  • However, co-localization of HLA-E and HLA-G didn't change when cultured with stimulated PBMCs. (iupui.edu)
  • Human PBMCs were labeled with CFSE. (biolegend.com)
  • The "hgh-chip" will then be thoroughly evaluated by performing numerous gene expression experiments on hgh-treated and untreated leukocyte subsets, cultured PBMCs as well as PBMCs obtained from hgh-patients. (wada-ama.org)
  • Human granulocytic anaplasmosis is a tickborne rick- chemical tests for Ehrlichia chaffeensis , the causative ettsial infection of neutrophils caused by Anaplasma agent of human monocytic ehrlichiosis (HME) were nega- phagocytophilum . (cdc.gov)
  • This coincided with less fungal association with leukocytes, especially neutrophils. (frontiersin.org)
  • The lower neutrophil number in murine blood only partially explains insufficient infection and filamentation control, as spiking with murine neutrophils had only limited effects on fungal killing. (frontiersin.org)
  • Strains of SIV have been successfully cultured in human lymphocyte cell lines (e.g. (cdc.gov)
  • SIV appears to be primarily tropic for CD4 (T4)-positive leukocytes and has not been successfully propagated in B-lymphocyte cell lines (e.g. (cdc.gov)
  • It thus defines L-selectin as a human lymphocyte homing receptor for peripheral lymph node HEV. (bdbiosciences.com)
  • The purified BDC elicited strong allogeneic mixed leukocyte reactions and HLA classes II- and I-restricted antigen-specific primary immune responses. (nih.gov)
  • Perform human leukocyte antigen (HLA) or DNA typing in patients who are potential candidates for allogeneic transplantation . (medscape.com)
  • An elective genetic test analyzes selected sites in the human genome while an elective genomic test analyzes the entire human genome. (wikipedia.org)
  • In recent years the development of high-throughput or next-generation sequencing has dramatically lowered the cost of DNA sequencing permitting laboratories to evaluate all 20,000 genes of the human genome at once through exome sequencing and whole genome sequencing. (wikipedia.org)
  • genome double-stranded rnas isolated from purified human reovirus (serotype 3) and rotavirus (wa strain) were modified at the 3' termini by addition of oligo(c) approximately 15 with t4 rna ligase. (liverpool.ac.uk)
  • After confirming SSH-genes to be differentially expressed in a series of microarray experiments, oligonucleotide probes will be designed for both SSH-genes and the candidate genes identified along gene expression profiling using whole genome/leukocyte relevant arrays. (wada-ama.org)
  • Genetic testing for a variety of disorders has seen many advances starting with cytogenetics to evaluate human chromosomes for aneuploidy and other chromosome abnormalities. (wikipedia.org)
  • Since the genetic code was deciphered much has been learnt about the chromosome structure shared by all organisms from yeast to human. (biomedcentral.com)
  • The investigation of a DNase-hypersensitive site upstream of the CD7 gene on chromosome 17q25 has led to the discovery of a novel human gene designated K12 (SECTM1, the HGMW assignment). (consensus.app)
  • 2 ] in 1994 and is defined by the expression of a variety of cell surface antigens, including the stem cell marker CD34, the pan-leukocyte marker CD45, and monocyte markers CD14 and CD11. (ersjournals.com)
  • Infection enhancement of dengue type 2 virus in the human U-937 monocyte line by antibodies to Flavivirus cross-reactive determinants. (microbiologyresearch.org)
  • 90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral, but not mucosal lymphoid tissue. (bdbiosciences.com)
  • They can skin and can extend to the bone marrow, simulate benign skin disorders, making them blood lymphocytes, lymph nodes, and a diagnostic challenge for dermatologists1,2. (bvsalud.org)
  • In an effort to investigate the impact of RhuDex1 on lamina propria and autologous peripheral blood leukocytes in a standardized setting resembling the in vivo scenario, we employed an ex vivo human organ culture model of intestinal inflammation [15]. (deubiquitinaseinhibitor.com)
  • It is believed that adhesive interactions between selectins on the blood vessel wall and selectin ligands on the CTC surface facilitate metastatic progression, in a manner similar to the leukocyte adhesion cascade essential in the inflammatory response (3). (aiche.org)
  • We also observed host-dependent differences for interaction of platelets with C. albicans , showing enhanced platelet aggregation, adhesion and activation in murine blood. (frontiersin.org)
  • In a physiological range between 12.5 and 125 µg/ml, the acidic HMO fraction inhibited leukocyte rolling up to 24.0% and leukocyte adhesion up to 52.8% in a concentration dependent manner. (uni-giessen.de)
  • Similar results were obtained using biotinylated CMRF-56 mAb, an antibody identifying a comparable population in cultured PBMC. (nih.gov)
  • Expression system for standard: Tumor necrosis factor receptor superfamily member 18(TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. (innov-research.com)
  • Early reports by Blau and colleagues indicated that rabbit fetal lung tissue cultured on Englebreth-Holm-Swarm tumor membrane could support production of type II pneumocytes [ 7 ]. (biomedcentral.com)
  • Plasma from peripheral blood was collected to measure tumor necrosis factor-a (TNF-α) and interleukin-8 (IL-8) by ELISA and nitrite (NO3) using a Griess assay. (bvsalud.org)
  • The Working Group was not aware of any good rodent models that simulate the occurrence of acute myeloid leukemia in humans. (who.int)
  • Exposure to benzene elicits a spectrum of hematotoxicity ranging from reduction of peripheral blood cell counts to aplastic anemia and leukemia. (cdc.gov)
  • Methods: This study aims to demonstrate the use of HLA class I molecules in inducing human NK cell tolerance. (iupui.edu)
  • Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • an electron microscope study of wild human rotavirus replication in cell cultures. (liverpool.ac.uk)
  • morphological studies of rotavirus replication in cell cultures]. (liverpool.ac.uk)
  • This gene spans approximately 14 kb and encodes a 1.8-kb mRNA detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. (consensus.app)
  • Review of the peripheral blood smear confirms the findings from the CBC count. (medscape.com)
  • The duration of tuberculosis treatment depends on the regimen chosen, the patient's clinical and radiographical responses, smear and culture results, and susceptibility of Mycobacterium tuberculosis isolates from the patient or the suspected source case. (who.int)
  • Also, despite the induction of HLA-DR on cultured human fibroblasts, no increased binding of PBMLs after IFN-γ treatment was observed. (elsevierpure.com)
  • All patients exhibited elevated expression of CYP4F3A encoding the leukotriene B4 (LTB4) -hydroxylase critical in the inactivation of LTB4 in polymorphonuclear leukocytes (PMN) with a fold of induction between 3 and 71. (cdc.gov)
  • DS patients also present a T cell immunodeficiency causing functional defects of polymorphonuclear leukocytes, reduced chemotaxis, diminished phagocytic ability, defective oxidative response and abnormal bactericidal activity 3 . (bvsalud.org)
  • Also, SIV is distinct from simian T-cell lymphotropic virus type I (STLY-I) which shares extensive genomic sequences with human T-lymphotropic virus type I and is associated with T-cell lymphomas in nonhuman primates (12). (cdc.gov)
  • The cell tropism of SIV in culture depends partially on the strain of virus propagated and conditions of cell culture. (cdc.gov)
  • These findings suggest that cell fusion might have induced epigenetic SCE frequency changes possibly in the same way as modulation of SCE frequencies is known to occur in the human leukocyte series. (biologists.com)
  • Xia S, Sun Q, Zou Z, Liu Y, Fang X, Sun B, Wei S, Wang D, Zhang A, Liu Q. Ginkgo biloba extract attenuates the disruption of pro-and anti-inflammatory T-cell balance in peripheral blood of arsenicosis patients. (ijbs.com)
  • AIM: To identify CD4(+) T-cell epitopes within Der p 2 recognized by commonly expressed human leucocyte antigen (HLA) alleles. (ox.ac.uk)
  • Pyruvate kinase deficiency is an inherited red blood cell enzyme disorder that causes chronic hemolysis. (conduent.com)
  • Applying our model, we demonstrate that RhuDex1 is capable of blocking a human memory T cell response, providing evidence that RhuDex1 could be expected to also influence inflammatory responses in vivo. (deubiquitinaseinhibitor.com)
  • Animal modeling and human mechanistic data are summarized to support the view that vitamin D probably influences thymic negative selection, effector Th1 and Th17 pathogenesis and responsiveness to extrinsic cell death signals, FoxP3 + CD4 + T-regulatory cell and CD4 + T-regulatory cell type 1 (Tr1) cell functions, and a Th1-Tr1 switch. (frontiersin.org)
  • However, acute xenograft rejection (AXR), driven by immune cell activation,continues to be a barrier in pig-to-human xenotransplantation. (iupui.edu)
  • NK cell tolerance occurs naturally in utero where human leukocyte antigen (HLA)-E and HLA-G are present. (iupui.edu)
  • Expressing HLA-E/G in xenografts may provide immune protection from human NK cell cytotoxicity. (iupui.edu)
  • Conclusion and Impacts: Co-expression of HLA-E and HLA-G in 5GKO pECs significantly reduced human NK cell degranulation, compared to 5GKO.HLA-E, 5GKO.HLA-G, and 5GKO pECs. (iupui.edu)
  • The cell culture media were supplemented with different HMO fractions or oligosaccharide standards. (uni-giessen.de)
  • These early simple one-cell or two-cell culture models provided information related to physiologic function of cell types found in the upper and lower airways but did not allow for assessment of more complex cell-cell or cell-matrix interactions. (biomedcentral.com)
  • Patients with AML can have high, normal, or low white blood cell (WBC) counts. (medscape.com)
  • The polymorphonuclear leukocyte-neutrophil is a key cell type and an essential part of the host's inflammatory response. (bvsalud.org)
  • Although no reports of infection in humans have been documented, the expanding use of SIV as a model of HIV infection has raised concern about the potential risk of SIV transmission to humans. (cdc.gov)
  • The clinical course of this infection in monkeys, like that of AIDS in humans, is complicated by various opportunistic infections (7). (cdc.gov)
  • Subsequently meat, dairy products and water serve as key vehicles in the transmission of MAP infection to humans (from farm to fork) who have a genetic predisposition, thus leading to the development of CD. (wjgnet.com)
  • Dissemination of pathogens from a primary site of colonization or infection can occur via different routes, including lymphatic vessels and the blood stream ( 1 ). (frontiersin.org)
  • The tick-borne intracellular pathogen, Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae) causes human granulocytic anaplasmosis after infection of polymorphonuclear leucocytes. (biomedcentral.com)
  • Appropriate cultures should be obtained in patients with fever, or with signs of infection even in the absence of fever. (medscape.com)
  • To determine leukocyte functionalization with ES/TRAIL, fluorescent (FL) liposomes were exposed to human blood under shear flow, and leukocytes were isolated and analyzed for ES/TRAIL attachment using FC and confocal microscopy. (aiche.org)
  • Upon exposure to shear flow, ES/TRAIL liposomes in human blood were functionalized to the surface of leukocytes, as confirmed by confocal microscopy (Fig. 1A) and FC. (aiche.org)
  • monoclonal antibodies were derived against the sa11 simian, nic bovine, and wa human rotavirus strains and characterized by enzyme-linked immunosorbent assay, plaque neutralization, and hemagglutination inhibition. (liverpool.ac.uk)
  • The different P. parvum strains were grown in batch cultures in 2 trials under different experimental conditions to test the development of intra- and extracellular toxicity during growth. (rafinhibitors.com)
  • The hematogenous is by far the most frequent route for systemic infections of various bacterial and fungal pathogens, in the most severe cases leading to blood stream infections ( 2 ). (frontiersin.org)
  • We adapted the human whole-blood model to murine blood. (frontiersin.org)
  • Unlike human blood, murine blood was unable to reduce fungal burden and more substantial filamentation of C. albicans was observed. (frontiersin.org)
  • Our results reveal substantial differences between murine and human whole-blood models infected with C. albicans and thereby demonstrate limitations in the translatability of this ex vivo model between hosts. (frontiersin.org)
  • In mice, as the most commonly used laboratory animal for in vivo experiments, the blood volume that can be withdrawn repeatedly is very limited and thereby hampers in depth analysis of interactions within blood. (frontiersin.org)
  • People commonly use black seed for asthma, hay fever, diabetes, high blood pressure, eczema, weight loss, menstrual cramps, and many other conditions, but there is no good scientific evidence to support many of these uses. (medlineplus.gov)
  • For instance, mice don't personal a real homologue of human interleukin eight, and presumably the function of this cytokine in mice is subsumed by other molecules. (plasignaling.com)
  • Microarray technology will be used to search for changes in leukocyte gene expression which are directly related to the application of human growth hormone (hgh). (wada-ama.org)
  • During the proposed project new, nucleic acid-based analytical techniques will be used to gain insight into specific effects of hgh on gene expression of leukocytes. (wada-ama.org)
  • However, cultures without the presence of MSCs showed higher NK proliferation, expression of activation markers (CD16 and NKG2D) and related cytotoxic activity. (springer.com)
  • SIV can be isolated from a variety of tissues and body fluids-including blood, plasma, cerebrospinal fluid, and parenchyma tissues-of infected nonhuman' primates. (cdc.gov)
  • Further noteworthy, our final results show that the intestinal organ culture model represents a beneficial experimental system applicable in pre-clinical studies evaluating therapeutic compounds for intestinal inflammation. (deubiquitinaseinhibitor.com)
  • mixed human rotavirus electropherotypes were detected in stool samples from patients with acute gastroenteritis in santiago, chile. (liverpool.ac.uk)
  • This study was designed to characterize the nocturnal fall of blood pressure (NFBP) of elderly hypertensive patients (EH), with or without cerebrovascular disease or diabetes mellitus, as measured by automated blood pressure (BP) monitoring. (go.jp)
  • Enzyme replacement therapy (ERT) appears safe and effective for peripheral manifestations in patients with Gaucher disease types I and III, Fabry disease, mucopolysaccharidosis I (Hurler, Hurler-Scheie, and Scheie syndromes), mucopolysaccharidosis II (Hunter syndrome), mucopolysaccharidosis VI (Maroteaux-Lamy syndrome), and Pompe disease. (medscape.com)
  • Typically, a total treatment duration of 18-20 months and a treatment duration of 15-17 months after culture conversion are suggested for most patients, with the duration being modified according to the patient's response to therapy. (who.int)
  • To compare the activity and function of NE in the peripheral blood from DS patients with and without PD, assisted at the Center for Dental Assistance to Patients with Special Needs affiliated with the School of Dentistry of Araçatuba, Brazil. (bvsalud.org)
  • Purified NE were collected from peripheral blood of 22 DS patients. (bvsalud.org)
  • Next, we explored the potential for reovirus-induced immunotherapy using human co-culture models to mimic the myeloma-supportive BM niche. (bmj.com)
  • Within this regard, the scientific worth of an ani mal selleck SRC Inhibitors model depends upon how accurately it can mimic the human sickness, and an evaluation with the animal versions similarity to human disorder state is requisite. (plasignaling.com)
  • Simian immunodeficiency virus (SIV) belongs to the family Retroviridae (subfamily Lentivirinae) and is closely related to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2), the etiologic agents of acquired immunodeficiency syndrome (AIDS). (cdc.gov)
  • It is a WHO sociated with human immunodeficiency vi- collaborative centre in the Eastern Mediter- rus/acquired immune deficiency syndrome ranean Region (EMR). (who.int)
  • Since these early observations, fibrocytes have been implicated in the tissue remodelling that occurs in a variety of human diseases, including idiopathic pulmonary fibrosis, asthma, pulmonary hypertension, atherosclerosis and renal fibrosis [ 6 ]. (ersjournals.com)
  • This human TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. (innov-research.com)
  • Formaldehyde is carcinogenic to humans (Group 1) . (who.int)
  • Therefore, on the basis of the data available at this time, it was not possible to identify a mechanism for the induction of myeloid leukemia in humans. (who.int)
  • Interleukin-4-specific responses, both ex vivo and cultured to Der p 2 peptides, had a significant positive correlation with HDM-specific serum IgE. (ox.ac.uk)
  • Model design in the future will also allow for evaluation of multiple organ systems at once, providing a more holistic or whole-body approach to understanding human physiology and responses. (biomedcentral.com)