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  • progenitor
  • We have created a 3D microenvironment through electrospinning to study the impact of geometry and different extracellular proteins on the development of cardiac progenitor cells (Flk-1 +) from resident stem cells and their differentiation into functional cardiovascular cells. (naver.com)
  • Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche. (naver.com)
  • Here we explore the proof of concept that treatments based on transplants of human oligodendrocyte progenitor cells can replace or remodel myelin and, eventually, contribute to axonal regeneration in traumatic axonal injury. (biomedcentral.com)
  • We derived human oligodendrocyte progenitor cells from the human embryonic stem cell line H9, purified and characterized them. (biomedcentral.com)
  • We then transplanted these human oligodendrocyte progenitor cells into the deep sensorimotor cortex next to the corpus callosum of nude rats subjected to traumatic axonal injury based on the impact acceleration model of Marmarou. (biomedcentral.com)
  • At the time of transplantation, over 90 % of human oligodendrocyte progenitor cells expressed A2B5, PDGFR, NG2, O4, Olig2 and Sox10, a profile consistent with their progenitor or early oligodendrocyte status. (biomedcentral.com)
  • Human oligodendrocyte progenitor cells displayed a striking preference for white matter tracts and were contained almost exclusively in the corpus callosum and external capsule, the striatopallidal striae, and cortical layer 6. (biomedcentral.com)
  • Over 3 months, human oligodendrocyte progenitor cells progressively matured into myelin basic protein(+) and adenomatous polyposis coli protein(+) oligodendrocytes. (biomedcentral.com)
  • The injured environment in the corpus callosum of impact acceleration subjects tended to favor maturation of human oligodendrocyte progenitor cells. (biomedcentral.com)
  • They are especially useful for investigating the development of early hematopoietic progenitor cells (HPCs). (jove.com)
  • Differentiation of galactocerebroside + (GalC) and O4 + oligodendrocyte precursor or progenitor cells (OPC) from neural precursor cells has been reported using second trimester fetal brain. (jove.com)
  • In order to address this issue, we investigated how osteoblasts (OBs) can affect the molecular and functional phenotype of Hematopoietic Stem/Progenitor Cells (HSPCs) and vice versa. (jove.com)
  • Moreover, this procedure allows for the retrieval and characterization of cells enriched in the BM such as hematopoietic stem and progenitor cells (HSPCs) without sacrifice of mice. (jove.com)
  • Given that the cellular composition of peripheral blood is not necessarily reflective of proportions and types of stem and progenitor cells present in the marrow, procedures which provide access to this compartment without requiring termination of the mice are very helpful. (jove.com)
  • The use of femoral bone marrow aspiration is illustrated here for cytological analysis of marrow cells, flow cytometric characterization of the hematopoietic stem/progenitor compartment, and culture of sorted HSPCs obtained by femoral BM aspiration compared with conventional marrow harvest. (jove.com)
  • assays
  • However, our knowledge regarding human pluripotent stem cell glycosphingolipid expression was until recently mainly based on immunological assays of intact cells due to the very limited amounts of cell material available. (deepdyve.com)
  • cardiomyocytes
  • In this study, the functional contributions of Ca 2+ transporters to [Ca 2+ ] i decline in mouse embryonic stem cell-derived cardiomyocytes (mESCMs), a suitable model for investigation of early cardiogenesis, at various differentiation stages were investigated. (physiology.org)
  • It is, therefore, postulated that NCX would play a more prominent role in cytosolic Ca 2+ removal than the contribution of SERCA Ca 2+ uptake during early differentiation of cardiomyocytes. (physiology.org)
  • On the other hand, recent reports showed that functional SR and SR proteins are already present in embryonic cardiomyocytes and contribute to the regulation of [Ca 2+ ] i transients ( 24 , 25 , 33 ). (physiology.org)
  • A recent study provides more direct evidence showing that a majority (∼74%) of cytosolic Ca 2+ removal is mediated by SERCA in mouse embryonic cardiomyocytes 8.5-9.5 days old ( 32 ). (physiology.org)
  • MEF2C has been implicated as a calcium-dependent transcription factor that enhances survival and affects synapse formation of neurons as well as differentiation of cardiomyocytes. (jneurosci.org)
  • scaffolds
  • We found that the number of Flk-1 + cells was significantly higher in 3D scaffolds coated with laminin or vitronectin compared to colIV-coated scaffolds. (naver.com)
  • Both scaffolds and cells have been shown to play a crucial role in regenerating tissues that emulate structural and functional characteristics of native tissues. (springer.com)
  • Feeder-free self-renewal of human embryonic stem cells in 3D porous natural polymer scaffolds. (nih.gov)
  • mouse
  • It is envisaged that the development of methods enabling controlled differentiation of mouse ES cell counterparts from human blastocysts would enable the provision of an unlimited supply of tissue for cell and tissue transplantation therapies for the repair and replacement of diseased, injured, and senescent tissue. (currentprotocols.com)
  • Furthermore, derivation of mouse ES cells has allowed for the generation of thousands of gene‐targeted mouse mutants. (currentprotocols.com)
  • This unit describes protocols for isolating mouse embryonic stem cells and methods for propagating, freezing, and producing EBs from both mouse and human embryonic stem cells. (currentprotocols.com)
  • Using a growth cone collapse assay, we demonstrate that mouse embryonic stem cell-derived spinal motor neurons (ES-MNs) respond to ephrin-A5, Sema3f, and Sema3a in a concentration-dependent manner. (jneurosci.org)
  • MilliporeSigma offers a broad range of tools and technologies to culture mouse embryonic stem cells including the gold standard ESGRO/mLif supplement, ESGRO complete and ESGRO2i serum-free/feeder-free media. (sigmaaldrich.com)
  • Pluripotent ES cells can be purchased or isolated from mouse blastocysts. (sigmaaldrich.com)
  • For over a decade, stem cell researchers have trusted their cultures with ESGRO ® mouse LIF supplement for maintaining the pluripotent state of their mouse ES cell lines. (sigmaaldrich.com)
  • However, due to its undefined composition, lots of material must be pre-screened in order to ensure undifferentiated mouse ES cell phenotypes. (sigmaaldrich.com)
  • These nutrients include hormones, vitamins, the growth factors mLIF and BMP4, as well a selective GSK3β inhibitor for enhanced mouse ES cell growth and viability at clonal densities in serum-free conditions. (sigmaaldrich.com)
  • One day before transfection, mouse D3 ES cells were trypsinized and plated in DMEM plus 20% FBS and 1000 U/ml LIF in six-well tissue culture plates coated with gelatin at a density resulting in ∼90% confluency within 1 d. (jneurosci.org)
  • Erythroid
  • Moreover, clonogenic assay and long-term culture results showed that co-culture with OBs induces a strong increase in mono/macrophage precursors coupled to a decrease in the erythroid ones. (jove.com)
  • proteins
  • Blastocyst formation requires the proper expression and localization of tight junction, polarity, ion gradient and H 2 O channel proteins in the outer cell membranes. (biologists.org)
  • Members of several gene families are important for blastocyst formation, including claudins, zonal occludins (TJ proteins), occludins, jam proteins, cell polarity proteins, Na/K-ATPases and aquaporins. (biologists.org)
  • Skin Remodeling is the process that removes proteins and older cells from the skin thus removing scars, lesions, and wrinkles while smoothing the skin. (skinbiology.com)
  • MEF2 proteins are highly expressed in cardiac myocytes and induce myogenesis from precursor cells ( Black and Olson, 1998 ). (jneurosci.org)