Cultured in medium conditioned. Medical search. Frequent questions

Cultured meat is produced by culturing/proliferating animal muscle cells in vitro. (nature.com)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Three-dimensional (3D) in vitro culture can improve the performance and physiological relevance of immortalized and primary cells. (reprocell.com)
In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. (ejbiotechnology.info)
The application of in vitro propagation techniques may offer the possibility of producing large number of uniform plants for breeding programs and further field culture. (ejbiotechnology.info)
This research considered the remodelling aspects of these conditions using both in vitro and pre-clinical models. (surrey.ac.uk)

First, rat liver epithelial RL34 cells were cultured by adding Chlorella vulgaris extract (CVE) to inorganic salt solution. (nature.com)
The present study characterized hydrosalpinx epithelial cell culture and examined the effects of its conditioned medium (CM) on sperm motility, acrosome reaction and embryo development. (hku.hk)
Methods: Normal Fallopian tubes (n = 6) and hydrosalpinges (n = 9) were used to prepare epithelial cell culture and CM. Epithelial cell characterization was confirmed using electron microscopy. (hku.hk)
The established epithelial cell culture system may provide a model to further investigate the mechanisms underlying the toxic effects of HF on embryo development and the adverse effects on IVF outcomes. (hku.hk)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)

The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. (scielo.br)
Animal cells are generally cultured in basal media supplemented with fetal bovine serum (FBS) which provides the various factors essential for cell growth. (nature.com)
The culture supernatant of animal cells, also known as 'conditioned medium' (CM), contains various factors secreted by the cells themselves, such as growth factors and cytokines. (nature.com)
We hypothesized that by selecting optimal animal cells and using their CM to culture animal myoblasts, the cells could proliferate without the need for animal sera. (nature.com)
CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
Studies of a pckA-lacZ gene fusion indicated that when cells were grown in minimal media with various carbon sources, such as succinate, malate, pyruvate, lactate, or ethanol, under both anaerobic light and aerobic dark conditions, the pckA gene was induced in log phase, irrespective of the carbon source. (asm.org)
Polyethylene glycol is used to fuse adjacent plasma membranes, but the success rate is low, so a selective medium in which only fused cells can grow is used. (wikipedia.org)
This medium is selective for fused (hybridoma) cells. (wikipedia.org)
Only fused hybrid cells referred to as hybridomas, are able to grow indefinitely in the medium because the spleen cell partner supplies HGPRT and the myeloma partner has traits that make it immortal (similar to a cancer cell). (wikipedia.org)
Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)
Electron microscopy demonstrated that the CAL 27 cells treated with GEF under AAS culture conditions exhibited swelling of the cytosol and organelles with an increased number of autophagosomes and autolysosomes, but without chromatin condensation and nuclear fragmentation. (spandidos-publications.com)
Could perfusion reduce metabolic stress in your cultures and improve the functionality of your cells? (reprocell.com)
Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis. (reprocell.com)
Say goodbye to cell culture contamination nightmares and hello to happy cells. (coleparmer.com)
Culturing suspension cells can be tricky because they need specialized culture vessels and techniques to maintain their viability and growth. (coleparmer.com)
Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
These cells support the growth and maintenance of other cells in culture. (coleparmer.com)
Feeder cells can provide essential growth factors, nutrients, and signaling molecules to support the growth of cells that are difficult to culture on their own. (coleparmer.com)
For suspension cells, use conical tubes or specialized culture vessels to keep cells in suspension and prevent aggregation. (coleparmer.com)
For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)
And for feeder cells, use flasks or dishes with a proper surface and growth media to support their growth and function. (coleparmer.com)
Bioreactor flasks are specialized vessels used to culture cells at a large scale, making them a valuable tool for the production of mAbs. (coleparmer.com)
While hybridoma cells can be cultured in standard culture vessels, bioreactor flasks provide several advantages when scaling up the production process. (coleparmer.com)
What are the challenges of culturing primary cells compared to established cell lines? (coleparmer.com)
Culturing primary cells presents unique challenges compared to established cell lines. (coleparmer.com)
Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)
Conditioned media from the CBox exposed macrophage cultures was used to treat mouse endothelial cells (C166). (cdc.gov)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
At each passage, the cells were counted, photographed using an inverted phase-contrast microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA) and cyro-preserved in cyrovial tube for further culture and later for RNA analysis. (bvsalud.org)
After washing, 1x10ˆ6 cells were seeded and cultured in KGM (Lonza Group Ltd., Basel, Switzerland). (bvsalud.org)
Total RNA was extracted from the cells cultured in KGM after 1, 3 and 7 days. (bvsalud.org)
Researchers from Lund have developed a completely animal-free preparation for cell culture, a medium that can be used to grow human cells, including for cancer research. (lu.se)
We show that it is possible to grow cells successfully with a completely defined and animal product-free medium," says Stina Oredsson. (lu.se)

However, technical challenges persist in the standardization of culture growth conditions and differentiation protocols. (the-scientist.com)
more specifically, the effect of osteocyte-conditioned medium on myogenic differentiation. (reprocell.com)
The aim of this study was to detect the expression of molecules associated with Notch signallng pathway in SHED cultured in specific differentiation medium, namely, KGM. (bvsalud.org)

For most of these conditions, the diagnosis can be ascertained through clinical history and presentation, but, on occasion, biopsy may be necessary. (medscape.com)
His condition was septic with clinical symptoms of meningismus and pulmonary congestion. (cdc.gov)
Although colonization is more common in children, only a small percentage of positive cultures findings have clinical significance in the pediatric population. (medscape.com)
However, all cultures positive for M catarrhalis had clinical importance in adults. (medscape.com)

Method of cultivation: Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. (europa.eu)

We found that 20 (10.6%) of 188 patients with chronic sup- nities, and P. aeruginosa was the predominant species in purative otitis media in Angola were co-colonized with fluo- 10 (50%) of the patients. (cdc.gov)
We tested A. faecalis isolates against a series of select- hronic suppurative otitis media (CSOM) is a common ed antimicrobial drugs by using broth microdilution (on- condition in developing countries and in original pop- line Technical Appendix Table) and found 100% were sus- ulations such as the Inuit ( 1 ). (cdc.gov)
Some During studies of ear disharge caused by otitis media, patients in the geographic area also used cockroach paste, we detected the gram-negative bacillus Alcaligenes faeca- palm oil, sweet olive oil, sewing machine oil, or breast lis in addition to the commonly isolated bacterial species milk to prevent ear discharge. (cdc.gov)
Topical treatment using antimicrobial drugs in combi- in patients who had acute otitis media, peritonitis, and eye nation with keeping the ear canal clean and dry is the main- or urinary tract infections ( 4-6 ). (cdc.gov)
Although the commensal status of M catarrhalis in the nasopharynx is still accepted, the organism is a common cause of otitis media and sinusitis and an occasional cause of laryngitis. (medscape.com)
M catarrhalis is the third most common cause of otitis media and sinusitis in children (after Streptococcus pneumoniae and Haemophilus influenzae ). (medscape.com)
M catarrhalis is estimated to be responsible for 3-4 million cases of otitis media annually, with an associated health care cost (direct and indirect) of $2 billion each year. (medscape.com)
The most significant infections caused by M catarrhalis are upper respiratory tract infections (URTIs) such as otitis media and sinusitis in children and lower respiratory tract infections (LRTIs) in adults. (medscape.com)

The best antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa (MIC 90 μg/ml), and against Aspergillus fumigatus (MIC 130 μg/ml) were found in extracts from cultures grown in medium containing 3.1% (w/v) sucrose and 0.1% (w/v) sodium nitrate at pH 4.0 after 9 days of incubation. (scirp.org)
Cultures grown in medium without modification also showed antiparasitic and antitumoral activities after 9 days at pH 4.0, antifungal activity against Candida albicans at pH 4.0 - 7.0 (MIC 140 μg/ml), but against A. fumigatus just after 27 days of incubation. (scirp.org)
The strain, grown in the medium with 1-2% chicken feathers and a yeast extract supplement, produced keratinases of 32 KU and lower level of proteases, 6 PU. (scielo.br)
TOA-1, grown on a skim milk/yeast extract medium, biosynthesized a highly stable keratinolytic enzyme with an optimum activity at pH 11.0-11.5 and temperature of 70-75 °C. This 20 kDa serine protease exhibited a high specific activity towards keratin and lower towards casein. (scielo.br)
The hybridomas can be grown indefinitely in a suitable cell culture medium. (wikipedia.org)

The supernatant, containing the RL34 cell-secreted growth factors, was used as the conditioned medium (CM). This CM, with CVE added as a nutrient source, was applied to primary bovine myoblast cultures. (nature.com)
The next day, S. zooepidemicus was cultured from the CSF and four of the four blood culture bottles, leading to a primary diagnosis of meningitis and sepsis. (cdc.gov)

Predictive microbiology uses mathematical models to predict the growth of bacteria based on environmental conditions. (bvsalud.org)

Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen. (atcc.org)

The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. (scielo.br)
The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase. (scielo.br)
In Rhizobium species, a PEPCK-deficient mutant fails to grow on minimal medium with succinate or other citric acid cycle intermediates but can grow on glucose and glycerol as a sole carbon source, suggesting that PEPCK functions here as a key gluconeogenic enzyme ( 30 ). (asm.org)
Growth continued at slower rates on glucose and leucine as carbon and nitrogen sources respectively, and at the end of culture (185 hrs), leucine was completely exhausted. (ejbiotechnology.info)
The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)

The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)
In contrast to the influx of amino acids, the degradation of proteins is also the important intracellular mechanism for releasing free amino acids both under steady-state conditions and during cellular stresses. (spandidos-publications.com)

To prevent this, it's important to create single-cell suspensions by gently pipetting or using specialized culture vessels. (coleparmer.com)

This serum-free and grain-derived-nutrient-free medium promoted the proliferation of bovine myoblasts, the main cell source for cultured beef. (nature.com)
MLO-Y4 osteocytes were cultured in Alvetex scaffold in either perfused or static conditions (using a perfusion plate or well inserts respectively) and the resulting conditioned medium was added to C2C12 myoblasts. (reprocell.com)

LabX.com is the premier marketplace for buying and selling, new, used and refurbished Cell Culture Media and Reagents and other laboratory equipment. (labx.com)
The master cultures were maintained in the laboratory under constant aeration and constant illumination at 24 ± 1 °C. (europa.eu)
This report provides CLIAC recommendations for good laboratory practices for ensuring the quality of molecular genetic testing for heritable diseases and conditions. (cdc.gov)
These recommendations are intended for laboratories that perform molecular genetic testing for heritable diseases and conditions and for medical and public health professionals who evaluate laboratory practices and policies to improve the quality of molecular genetic laboratory services. (cdc.gov)
Send the isolate (or CIDT-positive sample, if unable to culture) to the state public health laboratory using appropriate shipping conditions. (cdc.gov)

Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
1,3-DNB can undergo biodegradation under aerobic and anaerobic conditions in water and soil. (cdc.gov)

Compared with pristine CB, CBox demonstrated 2-4-fold greater potential to generate free radicals under acellular, cellular and in vivo conditions. (cdc.gov)

For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. (atcc.org)
All samples were collected at mid-depth in the test chambers, placed in glass vials (completely filled with no headspace) and processed immediately for analysis or stored under refrigerated conditions until analysis. (europa.eu)

The findings indicate that young people's political conversation - their political acting - in social media could be a new form of political communication with its own unique characteristics, creating conditions for young people's political socialization. (budrich-journals.de)
Our findings will allow us to take a major step toward reducing production costs and environmental impacts, leading to an expansion of the cultured meat market. (nature.com)

The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. (scielo.br)

Results: The percentage of human motile sperm incubated in hydrosalpinx CM was significantly different from those in normal Fallopian tube (NFT) CM and modified human tubal fluid medium (hTF) (control) (P (hku.hk)
We are united in our efforts to under- example, it could address the relationship between language stand, explain and improve our world and the human condition. (lu.se)
In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)
But in 2018, researchers in Lund started using different human proteins needed for cell survival in a cell culture medium. (lu.se)
Macrophage-conditioned media was collected and applied to cultures of human pulmonary fibroblast line WI38-VA13 to induce myofibroblast transformation. (cdc.gov)

Any of a number of antimicrobial drugs may be used to treat M catarrhalis infection, depending on the need for use of oral or parenteral medication, the age of the patient, any underlying conditions present, the sensitivity of the organism, and the desired spectrum of coverage. (medscape.com)

The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)

On the other hand, they really hate the people who have health problems like heart conditions, epilepsy, cancer, etc. (truthout.org)
Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)

Sinsky spoke with HealthLeaders Media about her study. (healthleadersmedia.com)
This was considered to have had no adverse effect on the results of the study given that the increase in cell concentration in the control cultures exceeded the validation criterion given in the Test Guidelines. (europa.eu)
In one study, 9% of cultures positive for M catarrhalis in children younger than 5 years and 33% of isolates from children aged 6-10 years were found to be clinically significant. (medscape.com)

Furthermore, it is composed of nutrients derived from grains and heterotrophic microorganisms and fetal bovine serum (FBS), which will impact the sustainability of cultured meat in future. (nature.com)
Here, we developed a novel medium containing nutrients extracted from microalga and cell-secreted growth factors. (nature.com)
Here, we propose a new approach wherein the nutrients in the culture medium are provided by a microalgae extract, and the FBS is replaced by growth factors in the CM. Figure 1 illustrates the preparation process of our serum-free and grain-derived-nutrient-free medium. (nature.com)

B, Pairing the Alvetex Perfusion Culture Plate with Alvetex well inserts combines the advantages of medium flow with 3D cell culture. (reprocell.com)

Confirmation of the diagnosis of M catarrhalis infection is based on culture. (medscape.com)

It has worked very well, despite both animal ethics and scientific concerns - which is why no one has really tackled developing a medium with defined content and without animal proteins. (lu.se)

The Scientist brings together a panel of experts to discuss challenges and strategies for optimizing your pluripotent stem cell culture. (the-scientist.com)

Our medium is open source,' says Stina Oredsson, the researcher behind the breakthrough who chose to give away the 'recipe' so that more researchers can work with more scientifically accurate and animal-free methods. (lu.se)

The produced keratinolytic enzymes were mainly a combination of alkaline serine or thiol proteases, active at the optimum pH 9.4, 55 °C. Four main protease fractions of 62, 185, 139 and 229 kDa were identified in the crude culture fluid. (scielo.br)
Samples for blood and synovial fluid cultures were obtained and anti-microbial therapy with intravenous cefuroxime was started. (cdc.gov)

This document also describes revised pediatric definitions for two acquired immunodeficiency syndrome-defining conditions. (cdc.gov)

Considering the amount of global resources and energy consumed, and animal welfare issues associated with traditional meat production, cultured meat production has been proposed as a solution to these problems and is attracting worldwide attention. (nature.com)
This process requires significant amounts of culture medium, which accounts to a major portion of the production cost. (nature.com)
The issues with culture medium are inescapable in cultured meat production. (nature.com)
Culturing microalgae in industrial wastewater provides the dual benefits of nutrient removal which prevents eutrophication as well as low-cost biomass production 6 . (nature.com)
examined the environmental impacts of meat production using the life cycle assessment method and reported that producing cultured meat using microalgal nutrient sources has a drastically lower environmental impact compared to conventional meat production 8 . (nature.com)
The effect of culture conditions on the production of bioactive secondary metabolites by the endophytic fungus Arthrinium State of Apiospora Montagnei Sacc. (scirp.org)
A specific bioactive compound may have its production improved if the culture conditions parameters that affect or influence the production were known, which in turn makes the purification process easier. (scirp.org)
They also can control environmental conditions, such as temperature, pH, and oxygen levels, to optimize cell growth and antibody production. (coleparmer.com)

The pH value of the control cultures was observed to increase from pH 7.9 at 0 hours to pH 10.2 at 72 hours. (europa.eu)

Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. (bvsalud.org)
The medium was changed 3 days after culture and were sub-cultured once they reached 70% confluence. (bvsalud.org)

With a broad understanding of the political life - the political - and a recognition of space and place as central aspects determining young people's condition for political socialization, the use of stake in a political conversation between young people is analyzed as an approach to describe and discuss aspects of political socialization in a public room within the social media - the net community. (budrich-journals.de)
While acknowledging the legacy of earlier studies and research in Nordic culture, our aim is to approach the discipline in a new way. (lu.se)

Since that time, advances in scientific research and technology have led to a substantial increase both in the health conditions for which genetic defects or variations can be detected with molecular methods and in the spectrum of the molecular testing methods ( 1 ). (cdc.gov)
As the number of molecular genetic tests performed for patient testing has steadily increased, so has the number of laboratories that perform molecular genetic testing for heritable diseases and conditions ( 2,3 ). (cdc.gov)

Popular culture and its social context are deeply intertwined, with each influencing and reflecting the other. (historici.nl)
With technological advances in radio, television and cinematography in subsequent decades, the importance of entertainment media in ideological, social and moral proselytizing became more pronounced. (theinterim.com)
Africa has seen a renewed quest for democracy expressed itself in the streets, popular culture, the Internet, and social media. (lu.se)

Extremist political parties have declared the need to preserve "genuine" Scandinavian culture from the threat of outside influence. (lu.se)

CBox NPs were generated under controlled conditions (2 ppm O3 for 3 hours) in a custom developed inhalation exposure chamber. (cdc.gov)
1994). This pattern of localized toxicity emphasizes the impor- inhaled organic acid vapor, acrylic acid, than the olfactory epi- tance of local tissue dose for interspecies extrapolation and risk thelium of the rodent nasal cavity when the exposure conditions assessment. (cdc.gov)

Pulmonary fibrosis is a poorly understood pathologic condition. (cdc.gov)

When nature, as well as courses focused on specific literary genres, applying for the Master's in Literature - Culture - Media, themes or historical periods. (lu.se)

Hydrolysis is not expected to be an important fate process since aromatic nitro compounds are generally resistant to chemical hydrolysis under environmental conditions. (cdc.gov)
Society frequently blames the victim rather than addressing environmental conditions that contribute to obesity. (cdc.gov)

The Erasmus School of History, Culture and Communication (ESHCC) at the Erasmus University Rotterdam is pleased to announce a challenging PhD position that delves into the intricate dynamics and implications of (un)cancel culture within the realm of global popular culture, both in digital and non-digital spaces. (historici.nl)

Ultimately, the project aims to inform critical discussions on and strategies to navigate the complexities of cancel culture. (historici.nl)

Find informative news, articles, product insights and comparisons about Cell Culture Media and Reagents in our News & Insights area. (labx.com)
This innovative exploration offers insights into the multifaceted nature of cancel culture, while also providing an understanding of 'uncanceling. (historici.nl)

For example, preconception testing for cystic fibrosis and other heritable diseases has become standard practice for the care of women who are either pregnant or considering pregnancy and are at risk for giving birth to an infant with one of these conditions ( 4 ). (cdc.gov)

Photos and audio-visual recordings (hereafter: materials) may be used free of charge for all non-commercial publications that constitute promotion of Slovenia as a tourist destination, abroad or in the Republic of Slovenia , in accordance with these General Terms and Conditions. (slovenia.info)
An internationally oriented and varied job in an enthusiastic team, with good working conditions in accordance with the Collective Labour Agreement for Dutch Universities (CAO NU). (historici.nl)

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