Culture media conditions. Medical search. Frequent questions

The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. (scielo.br)
In the first report [2], researchers from Edinburgh and Oxford took cells from the mouse brain marked with transgene 1, and cultured them together with ES cells marked with a second transgene, 2. (i-sis.org.uk)
In the second report [3], mouse bone marrow cells marked with green fluorescent protein were found to fuse with ES cells in culture in the presence of the cytokine, interleukin-3, which is known to encourage cell fusion. (i-sis.org.uk)
But, although the mutation frequency of genes was much lower in ES cells, mutant ES cells accumulated with time in culture. (i-sis.org.uk)
Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
The latter limits the choice of cell culture models, possibly excluding cultures of normal, untransformed cells, which are the most interesting and versatile for basic research. (forschung3r.ch)
The further identification of serum components that are obviously required by cultured cells is of less high priority. (forschung3r.ch)
Of all the components taken into account for cultured cells, the lipids are the most neglected ones. (forschung3r.ch)
This 3D primary cell culture system prepared from embryonic rat brain cells (2-5) contains all types of brain cells (i.e., neurons, astrocytes, oligodendrocytes, and microglia) that are enabled to grow and mature in serum-free medium, and to form highly differentiated structures such as myelinated axons, synapses, and functional neuronal networks. (forschung3r.ch)
BIO-MPM-1 Medium is a multi-purpose, serum-free medium for culture of a wide variety of anchorage-dependent cells. (bioind.com)
After the addition of 2mM glutamine, BIO-MPM-1 Medium is ready to use for adherent cells. (bioind.com)
BIO-MPM-1 Medium is suitable for a wide variety of cells. (bioind.com)
Three-dimensional (3D) in vitro culture can improve the performance and physiological relevance of immortalized and primary cells. (reprocell.com)
Could perfusion reduce metabolic stress in your cultures and improve the functionality of your cells? (reprocell.com)
Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis. (reprocell.com)
The Se-enriched exopolysaccharide fraction significantly enhanced cell viability when incubated with normal (human umbilical vein endothelial cells, HUVEC) cells (but this effect was absent for malignant human cervical HeLa cells) and this fraction also protected the cells from oxidative stress conditions. (preprints.org)
Centromeric heterochromatin formation was augmented upon transfer to the rich medium in wild-type cells. (nih.gov)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
At each passage, the cells were counted, photographed using an inverted phase-contrast microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA) and cyro-preserved in cyrovial tube for further culture and later for RNA analysis. (bvsalud.org)
After washing, 1x10ˆ6 cells were seeded and cultured in KGM (Lonza Group Ltd., Basel, Switzerland). (bvsalud.org)
Total RNA was extracted from the cells cultured in KGM after 1, 3 and 7 days. (bvsalud.org)
Researchers from Lund have developed a completely animal-free preparation for cell culture, a medium that can be used to grow human cells, including for cancer research. (lu.se)
We show that it is possible to grow cells successfully with a completely defined and animal product-free medium," says Stina Oredsson. (lu.se)

more specifically, the effect of osteocyte-conditioned medium on myogenic differentiation. (reprocell.com)
The aim of this study was to detect the expression of molecules associated with Notch signallng pathway in SHED cultured in specific differentiation medium, namely, KGM. (bvsalud.org)

The present study characterized hydrosalpinx epithelial cell culture and examined the effects of its conditioned medium (CM) on sperm motility, acrosome reaction and embryo development. (hku.hk)
Methods: Normal Fallopian tubes (n = 6) and hydrosalpinges (n = 9) were used to prepare epithelial cell culture and CM. Epithelial cell characterization was confirmed using electron microscopy. (hku.hk)
The established epithelial cell culture system may provide a model to further investigate the mechanisms underlying the toxic effects of HF on embryo development and the adverse effects on IVF outcomes. (hku.hk)

Diagnosis is by demonstrating parasites in smears or cultures and increasingly by polymerase chain reaction (PCR)-based assays at reference centers. (msdmanuals.com)

Nous avons procédé à une analyse rétrospective des hospitalisations liées au VIH au centre médical de Tripoli en 2013. (who.int)

As the name implies, viable bioaerosol sampling involves collecting a bioaerosol and culturing the collected particulate. (cdc.gov)

The result of this study was that the sporulation was affected by differences of culture medium, Iight and isolate. (tubitak.gov.tr)
Send the isolate (or CIDT-positive sample, if unable to culture) to the state public health laboratory using appropriate shipping conditions. (cdc.gov)

THe pH range narrowed as the media aged, because the test media slowly equilibrated with atmospheric carbon dioxide due to aeration. (europa.eu)
One medium for both embryo handling in atmospheric conditions and culturing in a CO 2 Incubator. (sigmaaldrich.com)

LabX.com is the premier marketplace for buying and selling, new, used and refurbished Cell Culture Media and Reagents and other laboratory equipment. (labx.com)
Find Cell Culture Media and Reagents that are available to purchase online, or send a quote request to the vendor directly. (labx.com)
Find informative news, articles, product insights and comparisons about Cell Culture Media and Reagents in our News & Insights area. (labx.com)
Looking to sell an item related to Cell Culture Media and Reagents? (labx.com)
To enable embryo collection, manipulation, and transfer techniques, we offer a wide selection of mouse embryo media and reagents including M2, modified M16, FHM and proprietary KSOM mouse embryo media formulations. (sigmaaldrich.com)

By manipulating the media and culture conditions, different developmental fates were elicited. (i-sis.org.uk)
In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)

M2 embryo media is a modified Krebs-Ringer solution buffered with HEPES, is commonly used for collection of embryos and when handling them outside the incubator for extended periods of time. (sigmaaldrich.com)
FHM is typically used as an embryo medium for washing and handling embryos outside the CO2 incubators. (sigmaaldrich.com)
Classic embryo culture media based on modified Krebs-Ringer bicarbonate solution. (sigmaaldrich.com)

Results: The percentage of human motile sperm incubated in hydrosalpinx CM was significantly different from those in normal Fallopian tube (NFT) CM and modified human tubal fluid medium (hTF) (control) (P (hku.hk)
We are united in our efforts to under- example, it could address the relationship between language stand, explain and improve our world and the human condition. (lu.se)
In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)
But in 2018, researchers in Lund started using different human proteins needed for cell survival in a cell culture medium. (lu.se)
Macrophage-conditioned media was collected and applied to cultures of human pulmonary fibroblast line WI38-VA13 to induce myofibroblast transformation. (cdc.gov)

With a broad understanding of the political life - the political - and a recognition of space and place as central aspects determining young people's condition for political socialization, the use of stake in a political conversation between young people is analyzed as an approach to describe and discuss aspects of political socialization in a public room within the social media - the net community. (budrich-journals.de)
The findings indicate that young people's political conversation - their political acting - in social media could be a new form of political communication with its own unique characteristics, creating conditions for young people's political socialization. (budrich-journals.de)
The influence of the media has thus transformed the material conditions of life by people's habits. (bvsalud.org)

The strain, grown in the medium with 1-2% chicken feathers and a yeast extract supplement, produced keratinases of 32 KU and lower level of proteases, 6 PU. (scielo.br)
TOA-1, grown on a skim milk/yeast extract medium, biosynthesized a highly stable keratinolytic enzyme with an optimum activity at pH 11.0-11.5 and temperature of 70-75 °C. This 20 kDa serine protease exhibited a high specific activity towards keratin and lower towards casein. (scielo.br)
The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. (scielo.br)

Method of cultivation: Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. (europa.eu)
The master cultures were maintained in the laboratory under constant aeration and constant illumination at 24 ± 1 °C. (europa.eu)
This report provides CLIAC recommendations for good laboratory practices for ensuring the quality of molecular genetic testing for heritable diseases and conditions. (cdc.gov)
These recommendations are intended for laboratories that perform molecular genetic testing for heritable diseases and conditions and for medical and public health professionals who evaluate laboratory practices and policies to improve the quality of molecular genetic laboratory services. (cdc.gov)
Nonculturable organisms do not reproduce in the laboratory because of intracellular stress or because the conditions (e.g., culture medium or incubation temperature) are not conducive to growth. (cdc.gov)

Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)

Our medium is open source,' says Stina Oredsson, the researcher behind the breakthrough who chose to give away the 'recipe' so that more researchers can work with more scientifically accurate and animal-free methods. (lu.se)
To build up a network of South Asian and Swedish media researchers and journalists to discuss and exchange ideas about work methods, trends within the media industry and business models. (lu.se)

In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. (ejbiotechnology.info)
The application of in vitro propagation techniques may offer the possibility of producing large number of uniform plants for breeding programs and further field culture. (ejbiotechnology.info)
KSOM media allows outbred zygotes to overcome the two-cell block and supports in vitro and in vivo Development of various mouse strains. (sigmaaldrich.com)

Potato Ieaf extract agar (PLEA) was found as the most suitable medium for sporulation. (tubitak.gov.tr)
When sampling for culturable bacteria and fungi, the bioaerosol is generally collected by impaction onto the surface of a broad spectrum solid medium (agar), filtration through a membrane filter, or impingement into an isotonic liquid medium (water-based). (cdc.gov)

In 1931, Tarr and Hibbert published the first detailed study of the formation of bacterial cellulose by conducting a series of experiments to grow A. xylinum on culture mediums. (wikipedia.org)
Previous work showed that this culture system was ideal for the study of hormones, growth factors and other signaling molecules on cellular growth, maturation and homeostasis. (forschung3r.ch)
This was considered to have had no adverse effect on the results of the study given that the increase in cell concentration in the control cultures exceeded the validation criterion given in the Test Guidelines. (europa.eu)
Sinsky spoke with HealthLeaders Media about her study. (healthleadersmedia.com)
The Sweden-South Asia Media Project aims to study and report on the growing digital media landscape in South Asia and link these results together with the development of the Swedish media landscape. (lu.se)

The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. (scielo.br)

Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
1,3-DNB can undergo biodegradation under aerobic and anaerobic conditions in water and soil. (cdc.gov)

While bacterial cellulose is produced in nature, many methods are currently being investigated to enhance cellulose growth from cultures in laboratories as a large-scale process. (wikipedia.org)
Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
In this situation CO2 required for photosynthesis and growth would be derived from bicarbonate in solution which results in an increase in the pH of the culture. (europa.eu)
The protein content of BIO-MPM-1 is therefore less than 30 mg/L, and the medium contains no growth factors or hormones other than insulin. (bioind.com)
Predictive microbiology uses mathematical models to predict the growth of bacteria based on environmental conditions. (bvsalud.org)

The vasculitis reveals granulomatous inflammation to the vessel wall in small and medium-sized arteries and veins, making granulomatosis with polyangiitis a true vasculitis. (medscape.com)

Therefore the pH of the fresh media increased slightly at higher test concentrations. (europa.eu)

The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. (scielo.br)
The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase. (scielo.br)
The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)

For the purposes of the range-finding test, initial experiments and definitive test, additional sodium bicarbonate (250 mg/L) was added to the prepared culture medium prior to use. (europa.eu)
With a function similar to M2, the FHM medium is a modification of KSOM where part of bicarbonate is replaced with HEPES buffer. (sigmaaldrich.com)

Pulmonary fibrosis is a poorly understood pathologic condition. (cdc.gov)

The produced keratinolytic enzymes were mainly a combination of alkaline serine or thiol proteases, active at the optimum pH 9.4, 55 °C. Four main protease fractions of 62, 185, 139 and 229 kDa were identified in the crude culture fluid. (scielo.br)

Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): Not applicable. (europa.eu)

In his present investigation he used this serum-free aggregating brain cell cultures as a reference culture system. (forschung3r.ch)
This can be addressed by the use of a perfusion system that circulates a large quantity of media over or around the 3D culture. (reprocell.com)
When sampling is indicated, it is advisable to sample before, during, and after the sampling area is occupied, including times when the heating, ventilating, and air conditioning system is activated and inactivated. (cdc.gov)
The Reportable Events Table (RET) reflects what is reportable by law ( 42 USC 300aa-25 ) to the Vaccine Adverse Event Reporting System ( VAERS ) including conditions found in the manufacturers package insert. (cdc.gov)

Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. (bvsalud.org)

It has worked very well, despite both animal ethics and scientific concerns - which is why no one has really tackled developing a medium with defined content and without animal proteins. (lu.se)

Endocarditis is seen in a minority of these patients, and is typically culture negative, as the causative agent Tropheryma whipplei does not grow in ordinary culture media. (lu.se)

Using qualitative research methods, candidates interested in this PhD position investigate the role of digital (platforms, technologies) and traditional media in shaping and perpetuating cancel culture as a phenomenon. (historici.nl)

Since that time, advances in scientific research and technology have led to a substantial increase both in the health conditions for which genetic defects or variations can be detected with molecular methods and in the spectrum of the molecular testing methods ( 1 ). (cdc.gov)
As the number of molecular genetic tests performed for patient testing has steadily increased, so has the number of laboratories that perform molecular genetic testing for heritable diseases and conditions ( 2,3 ). (cdc.gov)

However, the foundations of a political culture necessary to consolidate liberal democracy are still weak in most African states. (lu.se)

When nature, as well as courses focused on specific literary genres, applying for the Master's in Literature - Culture - Media, themes or historical periods. (lu.se)
The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)

Adding FBS to a cell culture medium is like working with a black hole. (lu.se)

Ultimately, the project aims to inform critical discussions on and strategies to navigate the complexities of cancel culture. (historici.nl)

This innovative exploration offers insights into the multifaceted nature of cancel culture, while also providing an understanding of 'uncanceling. (historici.nl)

An internationally oriented and varied job in an enthusiastic team, with good working conditions in accordance with the Collective Labour Agreement for Dutch Universities (CAO NU). (historici.nl)
Photos and audio-visual recordings (hereafter: materials) may be used free of charge for all non-commercial publications that constitute promotion of Slovenia as a tourist destination, abroad or in the Republic of Slovenia , in accordance with these General Terms and Conditions. (slovenia.info)

Sample storage conditions before analysis: 50mL test solution were extracted three times with ethyl acetate (15 mL per extraction). (europa.eu)
Sample storage conditions before analysis: Duplicate samples were taken and stored at approximately - 20°C for further analysis if necessary. (europa.eu)

Most of these cell culture systems rely on the use of fetal bovine serum (FBS), a supplement of undefined and variable composition. (forschung3r.ch)
The ultimate goal remains to find a chemically defined culture medium appropriate for a broad range of cell culture systems. (forschung3r.ch)

In continuation of our research on the influence of selenium incorporation on the biosynthesis, structure, immunomodulatory and antioxidant activities of polysaccharides of fungal origin, we have isolated from a post-culture medium of Lentinula edodes a selenium (Se)-containing exopolysaccharide fraction composed mainly of a highly branched 1-6-α-mannoprotein of molecular weight 4.5x106 Da, with 15% protein component. (preprints.org)

The structure of this fraction resembled mannoproteins isolated from yeast and other mushroom cultures, but it was characterized by a significantly higher molecular weight. (preprints.org)

Popular culture and its social context are deeply intertwined, with each influencing and reflecting the other. (historici.nl)
With technological advances in radio, television and cinematography in subsequent decades, the importance of entertainment media in ideological, social and moral proselytizing became more pronounced. (theinterim.com)
Jacob Silverman offers a thorough thinking-through of the conditions that social-media culture has placed on modern life. (csmonitor.com)
Terms of Service: Social Media and the Price of Constant Connection by Jacob Silverman HarperCollins 448 pp. (csmonitor.com)
As becomes clear in Jacob Silverman's Terms of Service , a thorough thinking-through of the conditions that social-media culture has placed on modern life, the jump to such a conclusion isn't very far. (csmonitor.com)
Africa has seen a renewed quest for democracy expressed itself in the streets, popular culture, the Internet, and social media. (lu.se)

Have 4 connected wells, allowing for greater volume provision for highly metabolic monocultures, or active solute flow for paracrine or cell migration co-cultures. (reprocell.com)

The Erasmus School of History, Culture and Communication (ESHCC) at the Erasmus University Rotterdam is pleased to announce a challenging PhD position that delves into the intricate dynamics and implications of (un)cancel culture within the realm of global popular culture, both in digital and non-digital spaces. (historici.nl)

The pH value of the control cultures was observed to increase from pH 7.9 at 0 hours to pH 10.2 at 72 hours. (europa.eu)

Such conditions cause major limitations for nearly one in 10 of our citizens and account for 75% of the 2 ½ tril ion dol ars that this country spends every year on medical costs and care. (cdc.gov)

At the forefront of this intersection lies cancel culture, encompassing both cancel practices and cancel discourses. (historici.nl)

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