- However, a comprehensive evaluation of the involvement of RNA processing factors in the reprogramming of somatic mammalian cells is lacking. (nih.gov)
- Here, we used data from a large number of studies carried out in three mammalian species, mouse, chimpanzee and human, to uncover consistent changes in gene expression upon reprogramming of somatic cells. (nih.gov)
- The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
- Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
- Overview of Single Cell Combinatorial Indexing for labeling the transcriptome of single cells The cells of mouse embryos at day 9.5 to 13.5 were isolated and distributed into different wells of a microtiter plate. (mpg.de)
- Initially, all these cells are identical, but soon, they start to form the three germ layers, which represent the first stage of differentiation of the developing embryo. (mpg.de)
- Within a short time, the cells of the three germ layers are transformed into an embryo containing most of its major internal and external organs. (mpg.de)
- Together with researchers from Berlin, the American team used this method to study the activity of all cells from mouse embryos at the age of 9.5 to 13.5 days. (mpg.de)
- Chicken embryo fibroblast cells and chicken embryos were resistant. (cdc.gov)
- A variety of primary cells and established cell lines support the replication of flaviviruses: Green monkey (Vero), hamster (BHK-21), human (SW-13, HeLa), porcine (PS), and mosquito cell lines, as well as primary chicken and duck embryo cells have been used for flavivirus isolation and propagation in routine diagnostic applications. (cdc.gov)
- Importantly, recent advances in generating human embryo-like structures from human pluripotent stem cells and in vitro cultured human embryos have led to exciting new human-related embryological models that are promising for advancing human embryology. (birs.ca)
- From there, Dr. Brinster became interested in modifying the development of animals and their germ lines, and he went on to become the first person to show that it was possible to colonize a mouse blastocyst with stem cells from older embryos. (avma.org)
- Styrene -oxide is mutagenic in a series of in-vitro tests utilizing bacterial, yeast, and mammalian cells in culture. (cdc.gov)
- Scientists at the University of Cambridge have managed to create a structure resembling a mouse embryo in culture, using two types of stem cells - the body's 'master cells' - and a 3D scaffold on which they can grow. (cam.ac.uk)
- Once a mammalian egg has been fertilised by a sperm, it divides multiple times to generate a small, free-floating ball of stem cells. (cam.ac.uk)
- The particular stem cells that will eventually make the future body, the embryonic stem cells (ESCs) cluster together inside the embryo towards one end: this stage of development is known as the blastocyst. (cam.ac.uk)
- Both the embryonic and extra-embryonic cells start to talk to each other and become organised into a structure that looks like and behaves like an embryo," explains Professor Magdalena Zernicka-Goetz from the Department of Physiology, Development and Neuroscience, who led the research. (cam.ac.uk)
- Professor Zernicka-Goetz and colleagues found a remarkable degree of communication between the two types of stem cell: in a sense, the cells are telling each other where in the embryo to place themselves. (cam.ac.uk)
- Yamanaka worked to find new ways to acquire embryonic stem cells to avoid the social and ethical controversies surrounding the use of human embryos in stem cell research during the late twentieth and early twenty-first centuries. (asu.edu)
- Takahashi and Yamanaka placed the retrovirus-infected cells into cell culture with G418 antibiotic and cells to provide nourishment, called feeder cells. (asu.edu)
- Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
- Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
- Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
- The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
- In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
- Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
- [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
- Dr. Henri Woelders, of Wageningen Livestock Research (WLR) and the Centre for Genetic Resources, the Netherlands (CGN) presented on the potential of cryopreservation and use of various types of germplasm in pigs and chicken, focussing on embryos, gonads, primordial germ cells and semen, and including some highlights from the EU Horizon 2020 IMAGE project (LINK). (wur.nl)
- Cryopreservation and transfer of gonads was shown to be an effective means in bird and mammalian species, while primordial germ cells offer possibilities and potential advantages in bird and fish species. (wur.nl)
- Embryonic stem cells (ESCs) from the 'inner cell mass' of an early embryo give rise to all future cells and tissue of an animal, including the primordial germ cells, which ultimately form the germ cells (sperm or oocytes). (wur.nl)
- DESCRIPTION (provided by applicant): Project Summary We propose to develop a non-invasive method to predict mammalian oocyte quality by analyzing the surrounding cumulus cells' gene expression patterns (cGEP). (sbir.gov)
- We find that differentiated mammalian cells operate with high DNA methylation stability, consistent with earlier reports. (nottingham.ac.uk)
- 13. Tuby H, Maltz L, Oron U. Low-Level Laser Irradiation (LLLI) Promotes Proliferation of Mesenchymal and Cardiac Stem Cells in Culture. (medline.ru)
- After 24 h of culture, ARS2-null blastocysts contained a significantly greater number of apoptotic cells than wild-type or heterozygous blastocysts. (monash.edu)
- Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
- Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
- Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
- Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
- Microgravity led to an overall reduction in the rate of blastocyst formation after 96 hours of culture, and closer examination of these blastocysts revealed that the differentiation of embryonic cells into trophectoderm-the tissue that nourishes the embryo and ultimately contributes to placenta formation-was markedly impaired. (medgadget.com)
- The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
- In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
- Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)
- Recently, we have determined that embryos cultured from the blastocyst stage release growth factors that induce the soft agar growth of NRK cells. (nebraska.edu)
- Created by Lewis Wolpert in the late 1960s, the model uses the French tricolor flag as visual representation to explain how embryonic cells can interpret genetic code to create the same pattern even when certain pieces of the embryo are removed. (asu.edu)
- Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
- A germ layer is a group of cells in an embryo that interact with each other as the embryo develops and contribute to the formation of all organs and tissues. (asu.edu)
- Platt followed the paths of cells in developing mudpuppy embryos to see how embryonic cells migrated during the formation of the head. (asu.edu)
- With her research, Platt challenged then current theories about germ layers, the types of cells in an early embryo that develop into adult cells. (asu.edu)
- The mouse genetics laboratory includes well-furnished infrastructure for culture of embryonic stem cells, cryopreservation of embryos, micro-manipulation of embryos, animal surgery, construction of transgenes & gene knock-out vectors, identification of transgenic & knock-out mice, and analysis of gene expression. (ccmb.res.in)
- This hypothesis was evaluated in skin fibroblasts cultured from heterozygous (+/ j ) and homozygous ( j/j ) UGT-deficient Gunn rats and in mouse embryo culture, with confirmation of direct N 3 -glucuronidation of phenytoin in Gunn rats in vivo . (aspetjournals.org)
- Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. (bvsalud.org)
- The TGF-like growth factors from embryos were initially observed in extracts prepared from mouse embryos at mid and late stages of gestation. (nebraska.edu)
- Schumacher A., Fisher B. Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study. (medline.ru)
- 5. Oh S.J., Gong S.P., Lee, S.T. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. (medline.ru)
- 6. Schumacher A., Fischer B. Influence of visible light and room temperature on cell proliferation in preimplantation rabbit embryos. (medline.ru)
- 8. Oh S.J., Gong S.P., Lee, S.T. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. (medline.ru)
- Utrecht can do ovum pick-up from mares and collaborate with Avantea, Italy for in vitro production and cryopreservation of embryos. (wur.nl)
- For his doctoral thesis, Dr. Brinster developed the first reliable in vitro culture system for early mammalian embryos. (avma.org)
- By 48 h of in vitro culture, null blastocysts invariably collapsed and failed to proliferate. (monash.edu)
- After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
- Most of our current knowledge of mammalian embryology is derived from studies of the mouse embryo, leading to astonishing discoveries of self-organizing and emergent properties ("the laws of development") manifest during embryonic development. (birs.ca)
- Furthermore, methods for preservation of endangered avian species and the development of transgenic birds using surrogate egg shell culture have been created by scientists across the globe. (wikipedia.org)
- Figure 2: The self-organization theory of early mammalian development. (nature.com)
- The mammalian embryo is a paradigm of regulative development and self-organization, making it a fascinating system for quantitative experimentation and analysis and mathematical modeling. (birs.ca)
- Using this method of embryo manipulation, he next worked out many aspects of the metabolism and development of eggs and early embryos. (avma.org)
- Understanding the very early stages of embryo development is of interest because this knowledge may help explain why a significant number of human pregnancies fail at this time. (cam.ac.uk)
- This is because early embryo development requires the different types of cell to coordinate closely with each other. (cam.ac.uk)
- However, in a study published today in the journal Science , Cambridge researchers describe how, using a combination of genetically-modified mouse ESCs and TSCs, together with a 3D scaffold known as an extracellular matrix, they were able to grow a structure capable of assembling itself and whose development and architecture very closely resembled the natural embryo. (cam.ac.uk)
- Comparing their artificial 'embryo' to a normally-developing embryo, the team was able to show that its development followed the same pattern of development. (cam.ac.uk)
- To do so, it would likely need the third form of stem cell, which would allow the development of the yolk sac, which provides nourishment for the embryo and within which a network of blood vessel develops. (cam.ac.uk)
- Professor Zernicka-Goetz recently developed a technique that allows blastocysts to develop in vitro beyond the implantation stage, enabling researchers to analyse for the first time key stages of human embryo development up to 13 days after fertilisation. (cam.ac.uk)
- She believes that this latest development could help them overcome one of the main barriers to human embryo research: a shortage of embryos. (cam.ac.uk)
- We are very optimistic that this will allow us to study key events of this critical stage of human development without actually having to work on embryos. (cam.ac.uk)
- From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development. (ivf.net)
- The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
- 3. Takenaka M., Horiuchi T., Yanagimachi R. Effects of light on development of mammalian zygotes. (medline.ru)
- Determining the functions of novel genes implicated in cell survival is directly relevant to our understanding of mammalian development and carcinogenesis. (monash.edu)
- These data indicate ARS2 is essential for early mammalian development and is likely involved in an essential cellular process. (monash.edu)
- Given the successful development of non-mammalian embryos in microgravity, these findings were surprising, and Wakayama and colleagues intend to pursue further gravity-manipulation studies to zoom in on the source of the developmental problem. (medgadget.com)
- Although Arl13b-which is required for cilium biogenesis and embryo development-is known to be mutated in patients with JS, the specific cilial and molecular basis of Arl13b function has been poorly understood. (medicalxpress.com)
- Cell Signaling During Mammalian Early Embryo Development one, Moreover. (markusfraedrich.de)
- Medienreferent am Kölner Dom The Cell Signaling During Mammalian Early Embryo Development in the term's fove and Choice of 20 to 30 developer of technical occult Users Do expressed national ander to unfollow Social notions, now as writing of letter mechanisms was the worth for the ornate delivery of falce thinkers. (markusfraedrich.de)
- Cell Signaling During Mammalian Early Embryo Development Ethnicity sine poultry in addition. (markusfraedrich.de)
- Ut groups Cell Signaling During Mammalian Early Embryo Development 2015 men, importance crates respondents. (markusfraedrich.de)
- The roles of hormones and growth factors during the early stages of mammalian development are poorly understood. (nebraska.edu)
- During the past decade, it has become apparent that embryos at several stages of development produce growth factors, some of which exhibit the properties of transforming growth factors (TGFs). (nebraska.edu)
- Cerebral organoids, three-dimensional cultures that model organogenesis, provide a new platform to investigate human brain development. (fsu.edu)
- Recording and contextualizing the science of embryos, development, and reproduction. (asu.edu)
- Myogenesis is the formation of muscle that occurs throughout an animal's development, from embryo to the end of life. (asu.edu)
- Changes in genes and regulatory elements are associated with exceptional mammalian traits, such as hibernation, that could inform therapeutic development. (bvsalud.org)
- The 5-HT neurotransmitter regulates important pathways of mammalian metabolism and is synthesized from the phenylalanine, tyrosine and tryptophan amino acids 5 . (bvsalud.org)
- Louvet-Vallee, S., Vinot, S. & Maro, B. Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. (nature.com)
- Subsequently, all cell nuclei were extracted and a molecular barcode specific to the respective embryo was introduced to the RNA molecules of each cell. (mpg.de)
- Each cell could thus be assigned a specific transcriptome, which in turn could be assigned to an individual embryo. (mpg.de)
- We investigated the susceptibility to Usutu virus ( Flavivirus ) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos. (cdc.gov)
- however, viral multiplication was detected in all mammalian cell types by immunohistochemical tests. (cdc.gov)
- We investigated the in vitro susceptibility of various cell cultures and embryonated eggs to Usutu virus infection. (cdc.gov)
- To 1-day-old confluent monolayers of the permanent cell lines and primary cell cultures, grown on the surface of chamber slides, the Austrian Usutu virus strain Vienna 2001-blackbird (GenBank accession no. (cdc.gov)
- Today, this technique continues to form the foundation for research on mammalian embryos, including technologies such as transgenic engineering, embryonic stem cell therapy, human in vitro fertilization, mammalian cloning, and knockout engineering. (avma.org)
- The oocyte is the largest and most mysterious cell in the body of mammalian organisms. (ivf.net)
- Takahashi and Yamanaka also experimented with human cell cultures in 2007. (asu.edu)
- Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
- Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
- Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
- The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
- This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
- Trastuzumab-pkrb is produced by recombinant DNA technology in a mammalian cell (Chinese Hamster Ovary) culture. (rxlist.com)
- Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
- The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)
- Microgravity-cultured embryos successfully reached the two-cell stage and yielded viable offspring upon implantation into female mice, but at a significantly lower rate than their 1G counterparts. (medgadget.com)
- Oliver Blacque and colleagues (University College Dublin) used C. elegans and mammalian cell culture systems to investigate Arl13b function. (medicalxpress.com)
- Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
- This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
- Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
- The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
- As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
- Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
- The Cell Signaling During Mammalian Early Embryo of an subject symbolization usually comes about a Contemplator of neighboring production. (markusfraedrich.de)
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- In the plows, the Cell Signaling During Mammalian Early Embryo of signup favored to be, as respective regulation writing others were and the believable forum were to restrict. (markusfraedrich.de)
- mythological For Educators OCW Educator Portal Search for Instructor Insights Search for Teaching Materials OCW Collections Instructor Insights by Department MIT Courses about Teaching and Education K-12 OCW Highlights for High School MIT+K12 Videos Higher Ed Teaching Excellence at MIT MIT Undergraduate Curriculum MapResidential Digital instructions take not Borrow a Cell Signaling During Mammalian Why See? (markusfraedrich.de)
- 9 million legs or traditionally 31 Cell Signaling During Mammalian Early Embryo of the occult voting newsletter. (markusfraedrich.de)
- and SIC at Lisbon area and Faro) the EuBI users will find expertise in analysis of 2D/3D cell cultures, small model organisms and biomaterials from advanced light microscopy techniques and transmission electron microscopy. (eurobioimaging.eu)
- Although the precise developmental stage when production of these factors begins has not been established, the data suggest that these factors are released by cell types associated with early postimplantation embryos. (nebraska.edu)
- We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
- By 1981, he and Richard D. Palmiter, PhD, a professor of biochemistry at the University of Washington School of Medicine, were able to show for the first time that new genes could be introduced into the mammalian genome. (avma.org)
- To understand regulation of gene expression and to delineate the function of mammalian genes, we routinely create transgenic & gene knockout mice by performing DNA microinjection into mouse eggs, and genetic manipulation of mouse embryonic stem cells. (ccmb.res.in)
- With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
- Driever, W. & Nüsslein-Volhard, C. A gradient of bicoid protein in Drosophila embryos. (nature.com)
- Driever, W. & Nüsslein-Volhard, C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. (nature.com)
- The Casa Matemática Oaxaca (CMO) will host the "Modeling and Engineering of the Mammalian Embryo" workshop in Oaxaca, from June 5 - June 10, 2022. (birs.ca)
- Koichu B., Schwarz B. B., Heyd-Metzuyanim E., Tabach M. & Yarden A. (2022) Learning, Culture and Social Interaction. (weizmann.ac.il)
- Shell-less chick embryo culture is the process of growing chick embryos in vitro, without their protective egg shells, for scientific observation. (wikipedia.org)
- Chick embryos and other avian embryos have been used as biological models to visualize the developmental stages of embryos for education and to perform embryological manipulations. (wikipedia.org)
- The technique was used in India to scrutinize glucose-induced deformities in chick embryos. (wikipedia.org)
- Styrene -oxide is embryotoxic and teratogenic in chick embryo. (cdc.gov)
- His claim was based on chick-heart tissue cultures in his laboratory that seemed to be able to proliferate forever. (asu.edu)
- Figure 1: Classic models for lineage segregation in the early mouse embryo. (nature.com)
- Piotrowska, K. & Zernicka-Goetz, M. Role for sperm in spatial patterning of the early mouse embryo. (nature.com)
- In the current issue of the journal Nature , the scientists describe how the method has been improved and applied to mouse embryos in order to create an 'atlas' of gene activity during mouse organogenesis. (mpg.de)
- The mouse ARS2 protein is predominantly localized to the nucleus, and this nuclear localization is ablated in ARS2-null embryos, which in turn die around the time of implantation. (monash.edu)
- P53 depletion in naive hPSCs increased their contribution to mouse-human cross-species chimeric embryos upon priming and differentiation. (janelia.org)
- This application proposes to address several important questions concerning TGF-like growth factors released by early mouse embryos: 1) What are their identities? (nebraska.edu)
- Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
- In vitro fertilization (IVF) involves combining an egg and sperm within a laboratory setting to create an embryo. (ivf.net)
- Chromosomal aberrations and sister chromatid exchanges are detected in cultures of human lymphocytes. (cdc.gov)
- Prepared in tissue culture grade water. (sigmaaldrich.com)
- The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
- [6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
- Butylene-oxide is reported as mutagenic in in-vitro Salmonella, Klebsiella, and mammalian all point mutation assays. (cdc.gov)
- Also, recent advances in pig embryo cryopreservation were shown and it was demonstrated how mathematic modelling of cellular events during embryo cryopreservation are extremely helpful. (wur.nl)
- After a brief incubation period, the embryo is placed into the uterus, where it may implant and initiate a pregnancy. (ivf.net)
- Mares were killed 7.5-8.5 days after transfer and the uterus and oviducts flushed for embryo recovery. (bioone.org)
- Previous attempts to grow embryo-like structures using only ESCs have had limited success. (cam.ac.uk)
- Because of the large numbers, this work provides insights into factors affecting success rates of ovum pick-up and in vitro embryo production. (wur.nl)
- Currently, embryos are developed from eggs donated through IVF clinics. (cam.ac.uk)
- Mesoscopic imaging of live or cleared samples of multiple biological models, like 3D organoids, fish (Danio) and mammalian embryos, can be observed by SPIM, OPT, SPIM-OPT and micro-CT, including multiple Zeiss Z.1 light-sheet systems, the ultra-microscope and home-built SPIM-OPT for cm-large samples. (eurobioimaging.eu)
- In addition, the culture was immunofluorescently labeled with Cy2 conjugated to antibodies that target peroxisomal membrane protein 70 ( PMP 70 ), an abundant and integral membrane component of peroxisomes. (fsu.edu)
- Coimbra holds a consolidated expertise in calcium imaging in primary neuronal cultures, organoids, and organotypic neuronal slices. (eurobioimaging.eu)
- Scientists have designed drug delivery tests in mammalian embryos to treat degenerative diseases. (wikipedia.org)
- These findings can be integrated in a new unified framework that regards the early mammalian embryo as a self-organizing system. (nature.com)