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  • Embryo Transfer
  • I was awarded a scholarship in 1988 by the Nordic Ministry Council and spent 3 months studying bovine embryo production in vitro and embryo transfer at the Royal Veterinary and Agricultural University in Copenhagen, Denmark. (ibream.org)
  • gametes
  • Because generation of transgenic and gene-targeted mouse mutants is costly and can take long periods of time, manipulation of female gametes in vitro is a more economical and time-saving strategy. (jove.com)
  • vivo
  • The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. (bioportfolio.com)
  • Although embryos resulting from IVF were less successful in establishing pregnancy than appropriate in vivo fertilized controls, optimization of culture and transfer techniques enabled minimization of embyronic losses and permitted valid use of these techniques. (uwo.ca)
  • Current work focuses on utilising state-of-the-art proteomic techniques and novel in vivo cell imaging systems to investigate the interaction between ram spermatozoa, seminal plasma and the ewe's reproductive tract. (edu.au)
  • recombinant
  • The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses. (bioportfolio.com)
  • In oocytes from hibernation-interrupted toads, the p34(cdc2) protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocytes dependence on a low environmental temperature during winter hibernation. (jove.com)
  • spindle
  • tubulin on the spindle fibers and asters in oocytes after taxol treatment. (jove.com)
  • Our data suggest that ERK3 is crucial for spindle stability and required for the metaphase-anaphase transition in mouse oocyte maturation. (jove.com)
  • In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. (jove.com)
  • cells
  • Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. (jove.com)
  • The soft agar colony formation assay is a well-established method for characterizing this capability in vitro and is considered to be one of the most stringent tests for malignant transformation in cells. (jove.com)
  • Tregs can be generated from naive CD4 T cells in vitro with TCR- and co-stimulation in the presence of TGFβ and IL-2. (jove.com)
  • Culturing cells in a three dimensional hydrogel environment is an important technique for developing constructs for tissue engineering as well as studying cellular responses under various culture conditions in vitro. (jove.com)
  • While this technique is very attractive, it has limitations, such as a theoretical risk of reintroducing malignant cancer cells within the graft. (ac.be)
  • These cells could then be differentiated into the affected cell type of the patient and studied in vitro, or used to replace the damaged or missing cells. (jove.com)
  • The availability of various GFP-tagged proteins means that many cellular processes can be monitored in living cells over the course of minutes or hours, and using this technique, processes such as RNP transport, epithelial morphogenesis, and tissue remodeling have been described in great detail in Drosophila oocytes 1,2 . (jove.com)
  • undergoes
  • Previously, follicles were successfully cultured in alginate, an inert algae-derived polysaccharide that undergoes gelation with calcium ions 6-8 . (jove.com)
  • For several primary oocytes that complete meiosis I each month, only one or a few functional oocyte, the dominant follicles, completes maturation and undergoes ovulation. (wikipedia.org)
  • cleavage
  • No significant difference between all 3 culture media was recorded regarding cleavage rates, showing that culture media had no effect on the subsequent cleavage. (ufs.ac.za)
  • Procedures
  • Throughout these industries, there are no strict guidelines as to the in vitro handling methods for these procedures, leading to the question of whether different in vitro conditions influence the outcomes of semen analyses and ultimately if this impacts upon clinical decision making. (edu.au)
  • Methods
  • Here, we describe methods to isolate prophase-arrested oocytes from mice and for microinjection. (jove.com)
  • chromosome
  • Historically, chromosome-spreading techniques are used for counting chromosome number 6 . (jove.com)
  • Depleting maternal HDAC2 results in hyperacetylation of H4K16 as determined by immunocytochemistry-normal deacetylation of other lysine residues of histone H3 or H4 is observed-and defective chromosome condensation and segregation during oocyte maturation occurs in a sub-population of oocytes. (prolekare.cz)
  • In addition, the results provide additional support that histone deacetylation that occurs during oocyte maturation is critical for proper chromosome segregation. (prolekare.cz)
  • arrest
  • Deletion of ERK3 by microinjection of ERK3 morpholino (ERK3 MO) resulted in oocyte arrest at the MI stage with severely impaired spindles and misaligned chromosomes. (jove.com)
  • Many studies have shown that cyclic adenosine-5-monophosphate (cAMP)-dependent protein kinase A (PKA) and G-protein-coupled receptor 3 (GPR3) are crucial for controlling meiotic arrest in oocytes. (jove.com)
  • Furthermore
  • These results implicate HDAC2 as the major HDAC that regulates global histone acetylation during oocyte development and, furthermore, suggest HDAC2 is largely responsible for the deacetylation of H4K16 during maturation. (prolekare.cz)
  • Alginate
  • Previous in vitro results indicated that alginate beads might be a useful vehicle for food iron fortification. (jove.com)
  • proteomics
  • Computational tools, expression and proteomics assays, and chromatin-immunoprecipitation-based techniques provide important clues for identifying mRNAs that are direct targets of a particular miRNA. (jove.com)
  • incidence
  • The resulting increased incidence of aneuploidy likely accounts for the observed sub-fertility of mice harboring Hdac2 −/− oocytes. (prolekare.cz)
  • mice
  • We report here that mice harboring Hdac1 −/+ / Hdac2 −/− or Hdac2 −/− oocytes are infertile or sub-fertile, respectively. (prolekare.cz)
  • protein
  • This technique encompasses protein precipitation, liquid-liquid extraction, and solid-phase extraction as a means of fractionating metabolites into four distinct classes. (jove.com)
  • Then, we exclude protein availability and ubiquitin carboxyl-terminal hydrolase enzyme activity as factors in the response of oocytes to winter hibernation. (jove.com)
  • tissue
  • Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. (jove.com)
  • Neonatal myocytes are a common cell source used for in vitro studies in cardiac tissue formation and engineering 4 . (jove.com)
  • processes
  • Standard microfabrication processes and bio-analytical techniques have been successfully utilized for decades in the computer and biological industries, respectively. (jove.com)
  • Changes in histone acetylation occur during oocyte development and maturation, but the role of specific histone deacetylases in these processes is poorly defined. (prolekare.cz)
  • Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. (jove.com)
  • conventional
  • We have applied a powerful and novel well-tempered ensemble parallel tempering (PT-WTE) technique along with conventional molecular dynamics (MD) simulation to investigate the molecular-level consequences of selective methionine oxidation of fibrinogen. (jove.com)
  • studies
  • Any material of choice may be introduced into the oocyte, but because meiotically-competent oocytes are transcriptionally silent 4,5 cRNA, and not DNA, must be injected for ectopic expression studies. (jove.com)
  • These studies are fundamental in nature and examine the requirements for maturation and fertilisation of oocytes and viable growth of embryos. (edu.au)
  • respectively
  • A total of 1405 presumptive zygotes were randomly allocated to the 3 different culture media (481, 461 and 463 zygotes in KSOM, SOF and CR1, respectively). (ufs.ac.za)
  • However
  • However, it is unclear how gonadotropins modulate these factors to regulate oocyte maturation, especially by gap junctional communication (GJC). (jove.com)