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CollagenCollagen Type IConnective Tissue Growth FactorFibrillar CollagensCollagen Type IIICollagen Type IVCollagen Type IIReceptors, CollagenCollagen Type VICollagen Type VImmediate-Early ProteinsExtracellular MatrixIntercellular Signaling Peptides and ProteinsFibroblastsCollagen DiseasesProcollagenCollagen Type XVIIIHydroxyprolineCollagen Type XICells, CulturedFibrosisExtracellular Matrix ProteinsTransforming Growth Factor beta1Transforming Growth Factor betaCartilageSkinHydroxylysineBasement MembraneFibronectinsRNA, MessengerGelsFibril-Associated CollagensCollagen Type XTendonsCollagen Type XIIMicrobial CollagenaseCollagen Type VIIConnective TissueProteoglycansPepsin ACartilage, ArticularCollagenasesCattleLamininNon-Fibrillar CollagensCollagen Type VIIIChondrocytesWound HealingCell AdhesionGene Expression RegulationCysteine-Rich Protein 61ElastinOsteogenesis ImperfectaMicroscopy, ElectronGrowth SubstancesProcollagen-Lysine, 2-Oxoglutarate 5-DioxygenaseProlineAminopropionitrileDecorinMolecular Sequence DataCorneaImmunohistochemistryBone and BonesGene ExpressionMatrix Metalloproteinase 1Collagen Type XIIIIntegrin alpha1beta1Reverse Transcriptase Polymerase Chain ReactionAmino Acid SequencePeptide FragmentsChick EmbryoDermisMicroscopy, PolarizationMicroscopy, Electron, ScanningSignal TransductionIntegrinsConnective Tissue CellsCell MovementDisease Models, AnimalGelatinStress, MechanicalHSP47 Heat-Shock ProteinsProcollagen-Proline DioxygenaseBlotting, WesternTensile StrengthCell LineGlycosaminoglycansProtein-Lysine 6-OxidasePulmonary FibrosisTime FactorsTissue EngineeringActinsCell Culture TechniquesScleroderma, SystemicElectrophoresis, Polyacrylamide GelCorneal StromaBase SequenceMatrix MetalloproteinasesIntegrin alpha2Glomerular Mesangium
ConnectiveProteinsFibrosisFibroblastsFibroblastDepositionFibrillarProliferation and collagenFibronectin synthesisProteinImmunohistochemistryPathwaysVEGFContractionGenesCCN2SynthesisEndothelialFibroticIntegrinsTGFB1ActinCol1ProfibroticRemains unclearExpressionCellularInduceTypeManifestationProteoglycansReceptorsStimulateWoundCellsTissuesCellMatrixSkinInclude
Connective12
Proteins12
  • In addition, CTGF also binds growth factors and extracellular matrix proteins. (wikipedia.org)
  • Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
  • Extracellular matrix is a fibrillar network composed of structural (collagens) and adhesion (fibronectin) proteins crucial for tissue architecture and biological pathway Histol Histopathol 27:1515-1528, 2012 . (springer.com)
  • This fibrillar network is composed of many structural proteins, such as collagens, and adhesion proteins, including fibronectin and proteoglycans (perlecan, decorin) [ 13 , 14 ]. (springer.com)
  • UV radiation that causes oxidative stress and hence aging induces the expression of matrix metalloproteinases (MMPs) that degrade dermal collagen and other proteins from the extracellular matrix (Caravalhaes Lago, 2019). (promocell.com)
  • SMAD MifaMurtide proteins will be the major substrates of TGF-β1 receptors [19] whereas we previously discovered that TGF-β1 up-regulated CTGF manifestation had not been via SMAD pathways in rabbit corneal wound curing [14]. (bio2009.org)
  • B) Endogenous IGFBP\4 decreases the TGF\?Cinduced ECM proteins fibronectin, collagen, and tenascin\C in fetal adult and lung lung fibroblasts. (museopedrogocial.org)
  • Heightened production of collagen and additional matrix proteins underlies the fibrotic phenotype of systemic sclerosis (SSc). (antiviralbiologic.com)
  • Plays a role in the degradation of Extracellular domain matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. (enquirebio.com)
  • Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. (enquirebio.com)
  • Moreover, significant adjustments in the expression of genes encoding extracellular matrix proteins had been observed, including collagens kind I, VII and XIV, fibronectin and laminin. (interleukin-receptor.com)
  • Soon after the cells had been grown to full confluence, the fibroblasts had been cultured inside the presence of among the solutions for 7 days, with three media alterations, or 6 days, with two media modifications, every single in the continuous presence of ascorbate, CTGF/CCN2 proteins and anti-CCN2/ CTGF antibodies. (gpr44.com)
Fibrosis10
  • It is thought that CTGF can cooperate with TGF-β to induce sustained fibrosis and to exacerbate extracellular matrix production in association other fibrosis-inducing conditions. (wikipedia.org)
  • Overexpression of CTGF in fibroblasts promotes fibrosis in the dermis, kidney, and lung, and deletion of Ctgf in fibroblasts and smooth muscle cells greatly reduces bleomycin-induced skin fibrosis. (wikipedia.org)
  • In addition to fibrosis, aberrant CTGF expression is also associated with many types of malignancies, diabetic nephropathy and retinopathy, arthritis, and cardiovascular diseases. (wikipedia.org)
  • Several clinical trials are now ongoing that investigate the therapeutic value of targeting CTGF in fibrosis, diabetic nephropathy, and pancreatic cancer. (wikipedia.org)
  • Both treatments attenuated HRHF-induced increases in CTGF and fibrosis by 6 weeks of task performance. (cdc.gov)
  • Myocardial fibrosis, characterized as interstitial fibroblast proliferation and excessive collagen deposition, is the structural basis of myocardial stiffness and the key process of cardiac function transformation from the compensatory phase to heart failure. (dovepress.com)
  • We found that fibrocytes respond to fibrogenic stimuli (AngII and CTGF) by increasing the expression of collagen and CTGF, an early molecular mediator of fibrosis, while also promoting fibrocyte differentiation. (dal.ca)
  • Moreover, we interestingly found overlaps of pathways between renal fibrosis and skin fibrosis, such as GO:1901890-positive regulation of cell junction assembly, as well as common regulatory genes, such as CTGF, which is the key molecule regulating fibrogenesis. (frontiersin.org)
  • While antibodies aimed against these goals are being examined in clinical studies, a short trial of TGF- antibody didn't show efficiency in SSc [9] [5], while CTGF antibody studies in fibrosis are simply starting ("type":"clinical-trial","attrs":"text message":"NCT01217632″,"term_id":"NCT01217632″NCT01217632). (antiviralbiologic.com)
  • Fibrosis driven by excess CTGF leads to scarring and blindness. (medscape.com)
Fibroblasts11
  • This is consistent with additional reports where TGF-β1 improved CTGF manifestation in human being corneal fibroblasts [12]. (bio2009.org)
  • CTGF takes on a critical part in mediating lots of the essential fibroproliferative ramifications of TGF-β1 in corneal fibroblasts. (bio2009.org)
  • There is certainly proof that TGF-β1 induced CTGF manifestation can be mediated through JNK in human being lung fibroblasts [25]. (bio2009.org)
  • In gingival fibroblasts the only real MAPK mediates the TGF-β1 activated CTGF manifestation was JNK [26]. (bio2009.org)
  • ERK mediates TGF-β1 induced CTGF manifestation in pores and skin fibroblasts [27]. (bio2009.org)
  • Inhibition of p38 could suppress collagen MifaMurtide We and CTGF expression induced by TGF-β1 in conjunctival fibroblasts [28] fibronectin. (bio2009.org)
  • Our Previous research show that TGF-β1 induced the activation of JNK in corneal fibroblast inhibition of JNK pathway can efficiently inhibit TGF-β1 induced CTGF manifestation and following corneal fibroblast proliferation and collagen over-expression in corneal fibroblasts [15]. (bio2009.org)
  • These effects will tend to be specific since we previously reported that proliferation and collagen creation were not combined in SSc fibroblasts and an upsurge in collagen creation was not because of elevated fibroblast proliferation [15]. (antiviralbiologic.com)
  • Our experiments uncovered that roscovitine coordinately inhibited the appearance of collagen, fibronectin and 19660-77-6 manufacture CTGF in regular and SSc fibroblasts. (antiviralbiologic.com)
  • Outcomes Inhibition of collagen and fibronectin appearance by roscovitine We motivated the consequences of CDK inhibitors on manifestation of collagen and fibronectin in confluent SSc and regular fibroblasts. (antiviralbiologic.com)
  • Fixation and Sirius Red Assay The Sirius Red dye-binding assay for measuring collagen accumulation in gingival fibroblasts was adapted from a prior study completed in osteoblasts [Tullberg-Reinert and Jundt, 1999]. (gpr44.com)
Fibroblast2
Deposition3
  • Results: The scores for the density buy MG-132 of α-SMA- and ED-1-positive cells, the staining of MCP-1 and osteopontin, interstitial collagen deposition and amounts of TGF-β1 were significantly reduced by MMF or Trip. (vegfrinhibitors.com)
  • Y-27632 inhibits keratocyte-to-myofibroblast transition, and its topical application after a superficial lamellar keratectomy elicits an altered wound healing response, with evidence of an embryonic-type deposition of collagen fibrils. (molvis.org)
  • Three primary pathologic features are found in scleroderma and include increased collagen deposition, perivascular mononuclear cell infiltration, and vascular abnormalities. (medscape.com)
Fibrillar6
Proliferation and collagen1
Fibronectin synthesis2
  • TGF-β1 performed an important part in the activation of quiescent corneal keratocytes [15] CTGF was induced by TGF-β1 and mediated the result of TGF-β1 on collagen fibronectin synthesis [16]. (bio2009.org)
  • [ 1 ] TGF-ß stimulates fibronectin synthesis and collagen contraction by RPE cells. (medscape.com)
Protein7
  • Members of the CCN protein family, including CTGF, are structurally characterized by having four conserved, cysteine-rich domains. (wikipedia.org)
  • IGF-1, collagen I (col-I), and collagen III (col-III) protein and mRNA levels were then detected via ELISA and qPCR, respectively. (hindawi.com)
  • TGF-β2 treatment upregulated the expression of Col1α1, Col4α2, FN, CTGF, TGM2, and TSP-1 mRNA and protein in cultured astrocytes. (arvojournals.org)
  • TGF-β2 is capable of inducing the expression of ECM and basement membrane components in cultured ONH astrocytes via CTGF and upregulated TSP-1, a protein naturally involved in the activation of latent TGF-β. (arvojournals.org)
  • A significant increase in ROS production and CTGF protein expression were observed at 100 mu g L-1 Sb-III. (anid.cl)
  • SSc continues to be associated with dysregulation of immune system replies and of cytokines along with upregulation of matrix protein such as for example collagen and fibronectin [3]. (antiviralbiologic.com)
  • RCS-01 is an autologous cell-based regenerative medicine product comprised of cells highly-expressive of type 1 collagen and other tissue-building protein. (replicel.com)
Immunohistochemistry3
  • Immunohistochemistry confirmed PCR results, and also showed greater increases of CTGF and collagen matrix in 9-week HRHF rats than 9-week HRNF rats. (cdc.gov)
  • Then α-smooth muscle actin (α-SMA) was examined by immunohistochemistry and western blotting, and the contractility of the seeded collagen gels was measured. (molvis.org)
  • Y-27632 eye drops (or vehicle only) were administered to eyes after a superficial keratectomy, and the tissue was examined by immunohistochemistry for α-SMA, collagen types I, II, and III, and keratan sulfate. (molvis.org)
Pathways2
  • Predicated on these results it had been hypothesized that MAPK pathways could mediate CTGF manifestation and corneal skin damage in corneal wound curing. (bio2009.org)
  • They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. (lookformedical.com)
VEGF2
  • The N-terminal half of CTGF interacts with aggrecan, the TSR domain interacts with VEGF, and the CT domain interacts with members of the TGF-β superfamily, fibronectin, perlecan, fibulin-1, slit, and mucins. (wikipedia.org)
  • Increasing levels of CTGF inactivate VEGF, and when the equilibrium between these 2 factors shifts to a certain threshold ratio, the neovascular membranes become more fibrotic and less vascular. (medscape.com)
Contraction1
Genes3
  • The effect of CTGF silencing on the TGF-β2-modulated expression of these genes was investigated by transfection of CTGF small interfering (si)RNA before TGF-β2 treatment. (arvojournals.org)
  • In comparing the expression of these genes with that of hepatic stellate cells (HSCs), we observed a similar pattern, although PSCs expressed type I collagen gene earlier than did HSCs. (elsevierpure.com)
  • We also observed enhanced expression of MMPs, ADAMTS, TIMP and CTGF, amongst other genes. (interleukin-receptor.com)
CCN24
  • Synthetic CTGF/CCN2 peptide RANCLVQTTEWSACSKT is usually a custom-made peptide and was bought from SynPep Corporation, Dublin, CA. Treatment of Cells Cells had been cultured in media described above within the extra presence of ascorbate (0.05 mg/ mL) beginning on day 0 of remedy protocols. (gpr44.com)
  • In addition, TGF-1 (10 ng/ml), CTGF/CCN2 (one hundred ng/mL), N-terminal CTGF/CCN2 (50 or 100 ng/mL), C-terminal CTGF/CCN2 (50 or 100 ng/mL) or anti-CTGF/CCN2 antibody (10 g/mL) with CTGF/CCN2 (100 ng/mL) had been utilized in experiments. (gpr44.com)
  • In treating with antibodies against CCN2/CTGF, antibodies (4 g/ml) were preincubated for 15 minutes 37C in media containing all other elements like CCN2/CTGF just before adding towards the confluent cell cultures to allow for antibody binding to CCN2/CTGF. (gpr44.com)
  • On the other hand, antibodies against integrins have been added into each and every nicely 15 minutes and incubated below 37C prior to adding CCN2/CTGF in an effort to permit antibody-integrin binding. (gpr44.com)
Synthesis2
  • Therefore, we investigated the relationship between mechanical stress and collagen synthesis in the present study. (hindawi.com)
  • Previous studies [ 6 - 9 ] have shown that mechanical stress promotes collagen I (col-I) and collagen III (col-III) synthesis which contributes to LFH. (hindawi.com)
Endothelial1
  • Ctgf-null mice also show defects in angiogenesis, with impaired interaction between endothelial cells and pericytes and collagen IV deficiency in the endothelial basement membrane. (wikipedia.org)
Fibrotic2
  • CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. (wikipedia.org)
  • CTGF is associated with wound healing and virtually all fibrotic pathology. (wikipedia.org)
Integrins2
  • ECM components (i.e., fibronectin or collagens) directly interact with membrane receptors (i.e., integrins), particularly, by influencing cell behavior. (springer.com)
  • Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI. (lookformedical.com)
TGFB11
  • Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. (enquirebio.com)
Actin1
  • Cell morphology and RNA expression level (connecting tissue growth factor, CTGF), alpha-SMOOTH MUSCLE ACTIN (alpha-SMA) and collagen I were analysed. (anid.cl)
Col12
  • Quantitative PCR (qPCR) analyses of HRNF muscles showed increased expression of Col1 in weeks 3-9, and CTGF in weeks 6 and 9. (cdc.gov)
  • Transfection of 10 nM CTGF siRNA inhibited the TGF-β2-induced upregulation of CTGF, Col4α2, Col1α1, TGM2, and FN, whereas TSP-1 expression was not reduced. (arvojournals.org)
Profibrotic1
Remains unclear2
  • However, the mechanism of mechanical stress in the formation of collagen remains unclear. (hindawi.com)
  • Nevertheless the signaling pathway of CTGF creation in corneal wound curing remains unclear. (bio2009.org)
Expression4
  • Other effects of increased oxidative stress and ROS production are a decrease in CTGF, TGF-beta, and type I procollagen expression as well as an age-associated chronic progressive increase in the proinflammatory status - this is termed 'inflammaging' (Zuo, 2019). (promocell.com)
  • MMF or Trip significantly reduced the mRNA expression of TGF-β1, CTGF, MCP-1 and osteopontin. (vegfrinhibitors.com)
  • A randomized, double-blind, placebo-controlled, single-centre, phase I clinical study evaluated RCS-01 produced statistically and clinically significant positive data revealing a nearly two-fold increase in gene expression of collagen-related biomarkers in the skin, after a single injection of RCS-01. (replicel.com)
  • With Y-27632 treatment, α-SMA expression increased and the collagen type III signal became in the weaker subepithelial area. (molvis.org)
Cellular1
  • Experience lifting, toning and smoothing facial massages followed by a cellular collagen mask on the face, neck and décolleté. (montage.com)
Induce1
  • We've previously demonstrated that manifestation MifaMurtide of TGF-β1 and CTGF improved significantly during corneal wound curing TGF-β1 could induce CTGF manifestation in vivo [14]. (bio2009.org)
Type9
  • Some medium from loaded and unloaded explants was immunoblotted for type II collagen, CTGF and TIMP3. (ox.ac.uk)
  • Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. (enquirebio.com)
  • Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. (lookformedical.com)
  • The alpha subunits of collagen type VI assemble into antiparallel, overlapping dimers which then align to form tetramers. (lookformedical.com)
  • Several forms of collagen type V exist depending upon the composition of the subunits that form the trimer. (lookformedical.com)
  • The C-terminal end of the alpha1 chain of collagen type XVIII contains the ENDOSTATIN peptide, which can be released by proteolytic cleavage. (lookformedical.com)
  • Collagen type XI is heterotrimer containing alpha1(XI), alpha2(XI) and alpha3(XI) subunits. (lookformedical.com)
  • After superficial keratectomies, collagen type I and keratan sulfate were unchanged by Y-27632 application. (molvis.org)
  • Collagen type II was not detected in Y-27632 or vehicle-only corneas. (molvis.org)
Manifestation4
  • JNK inhibitor significantly inhibited CTGF collagen and fibronectin We manifestation induced by TGF-β1 in THSF. (bio2009.org)
  • In corneal wound curing the JNK inhibitor considerably inhibited CTGF manifestation markedly improved the structures of corneal stroma and decreased corneal scar development but didn't possess a measurable effect on corneal wound curing in vivo. (bio2009.org)
  • Our outcomes indicate that JNK mediates the manifestation of CTGF and corneal skin damage in corneal wound curing and might be looked at as specific focuses on of medication therapy for corneal skin damage. (bio2009.org)
  • Consequently understanding systems regulating manifestation of CTGF improved by TGF-β1 can be of great importance to inhibit corneal skin damage. (bio2009.org)
Proteoglycans1
  • CTGF exerts its functions by binding to various cell surface receptors in a context-dependent manner, including integrin receptors, cell surface heparan sulfate proteoglycans (HSPGs), LRPs, and TrkA. (wikipedia.org)
Receptors1
Stimulate2
  • The collagen infusion is designed to strengthen and stimulate cells and reveals sublime, youthful, radiant skin. (montage.com)
  • Once in the vitreous cavity, the RPE cells undergo morphologic changes where they attain fibroblastlike activity, secreting growth factors that stimulate collagen and fibronectin production. (medscape.com)
Wound1
  • The age-related alteration of collagen-rich extracellular matrix - promoted by oxidative stress - impairs the skin's structural integrity and creates a tissue microenvironment that promotes age-related skin diseases such as poor wound healing and skin cancer. (promocell.com)
Cells4
  • Mechanical stress promotes human ligamentum flavum cells (LFCs) to synthesize multitype collagens, leading to ligamentum flavum hypertrophy (LFH). (hindawi.com)
  • At 100 mu g L-1, the number of collagen I-positive cells increased. (anid.cl)
  • These results demonstrate that an Sb-III concentration of 100 mu g L-1 is able to promote the induction of CTGF and collagen I along with the induction of ROS, which suggests a cytotoxicity of Sb-III in M-1 kidney collecting duct cells. (anid.cl)
  • Once collagen sheets form, the individual cells pull on them, leading to TRD. (medscape.com)
Tissues1
  • Collagens are crucial for the ECM architecture and particularly collagen I, III and VI are key components in animal tissues. (springer.com)
Cell2
  • CTGF is also important for pancreatic beta cell development, and is critical for normal ovarian follicle development and ovulation. (wikipedia.org)
  • RCS-01 is a patient-specific cell therapy in development to regenerate the extracellular tissue matrix under aging or sun-damaged skin characterized by fine wrinkles and loss collagen structure. (replicel.com)
Matrix1
Skin3
  • Lots of the skin damage ramifications of TGF-β1 are mediated by CTGF [3]. (bio2009.org)
  • TGF-β1 and CTGF are fundamental growth elements in regulating corneal skin damage [12] [13]. (bio2009.org)
  • Repair the signs of aging and bring luminosity back to your skin with this glycolic peel and collagen mask combination. (montage.com)
Include1