• Overall, this work demonstrates how the ensemble nature of macromolecular structure, revealed here by multitemperature crystallography, can elucidate allosteric mechanisms and open new doors for long-range control of protein function. (rcsb.org)
  • Serial crystallography is a new way of studying macromolecular structures using synchrotron and X-FEL sources around the world. (lightsources.org)
  • BioMAX, the first macromolecular crystallography (MX) beamline and one of the first beamlines in user operation, is designed to support all kinds of established crystallography methodologies. (lu.se)
  • LP3 has been granted beamtime at BioMAX (the X-ray macromolecular crystallography beamline of MAX IV). (lu.se)
  • The extracted parameters in terms of scanning speed, size of the imaged volume and achieved resolutions should provide a baseline for future users designing nano-Tomography experiments on the NanoMAX beamline. (lu.se)
  • To understand how this antibody hinders the spike protein, and to gather the information necessary to reproduce it, the team behind the current study used cryo-electron microscopy (cryo-EM) at the University of Washington Arnold and Mabel Beckman cryoEM center and X-ray crystallography performed at ALS beamline 5.0.2. (lbl.gov)
  • MicroMAX will become a micro-focusing beamline, which will allow investigations of micrometer sized protein crystals at room temperature using serial crystallography. (lu.se)
  • After a four-year construction period, the beamline will be ready for first experiments. (lu.se)
  • Our most popular diffractometer for chemical crystallography, configured with either single or dual microfocus sealed tube X-ray sources and an extremely low noise direct X-ray detection detector. (rigaku.com)
  • An entry-level single crystal X-ray diffractometer for Chemical Crystallography configured with microfocus sealed tube technology and a direct X-ray detection detector. (rigaku.com)
  • Spectacular performance combined with dual wavelength versatility, provides the perfect answer for high-capacity Chemical Crystallography labs or for X-ray facilities that support Chemical Crystallography and Protein Crystallography . (rigaku.com)
  • The final decisive option is to carry out more than one experiment, e.g. multiple X-ray crystallography experiments and ideally neutron crystallography. (rcsb.org)
  • The complementary technique of neutron protein crystallography has provided evidence of the protonation states of histidine and acidic residues in concanavalin A and also the correct orientations of asparagine and glutamine side chains. (rcsb.org)
  • Principles of X-ray crystallography, neutron crystallography, small angle X-ray and neutron scattering. (lu.se)
  • The MYTHEN single-photon-counting silicon microstrip detector has been developed at the Swiss Light Source for time-resolved powder diffraction experiments. (scienceopen.com)
  • We have piloted data search through heterogeneous data sources from a single interface across samples and experiments for Inner Shell Spectroscopy (ISS) and X-ray Powder Diffraction (XPD). (bnl.gov)
  • Weidenthaler, C. An in situ powder diffraction cell for high-pressure hydrogenation experiments using laboratory X-ray diffractometers. (mpg.de)
  • Our most popular diffractometer for Protein Crystallography , configured with a high-flux rotating anode X-ray source and an extremely low noise direct X-ray detection detector. (rigaku.com)
  • Automatic LCP (lipidic cubic phase) experiments are also possible. (lu.se)
  • His work is largely based on diffraction phenomena and combines computational, biochemical and biophysical experiments. (iucr.org)
  • Biochemical experiments have shown that the tigecycline molecule binds to the same site on 16S rRNA as tetracycline but in a different orientation and with greater affinity. (kenyon.edu)
  • BioSAXS is particularly powerful when combined with additional information from other biochemical and biophysical experiments such as crystallography. (stanford.edu)
  • The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)
  • Here, biophysical methods and X-ray crystallography were used to identify the real properties and behaviour of the peptides. (eurekalert.org)
  • At the LP3 crystallisation and biophysical laboratory, you can carry out a wide variety of robot-assisted crystallisation experiments in nano volumes as well as screen for a large number of buffer conditions to stabilize their sample. (lu.se)
  • These 'time resolved' nano crystal experiments are currently beyond the capabilities of the crystallographic method but remain the ultimate quest of many structural biology projects as they could reveal the complex structural changes of enzymes as they react in real time with their substrates. (ox.ac.uk)
  • TR-SAXS (Time-resolved SAXS) is a unique experiment to pursue the fast changes during the reaction (e.g. conformational changes of the sample in the msec to min time range). (stanford.edu)
  • Using a wide bandpass option, MicroMAX can be exploited also for time resolved crystallography down to the microsecond time resolution range. (lu.se)
  • Performs automatic imaging of crystallisation experiments at 20 °C using visible and UV light. (lu.se)
  • Recent technological breakthroughs, such as ultra-fast pulsed lasers, high-performance computing and crystallography of macromolecules, helped to improve the experimental tools of physicists, biologists, and chemists working, in particular, in the area of chemical and biological physics. (cecam.org)
  • The use of molecular mechanics calculations to supplement experimental data in standard X-ray crystallography and NMR refinements is discussed and it is shown that structures can be locally improved by the use of quantum chemical calculations. (lu.se)
  • It is an electron storage ring that runs at a constant beam current (Top-up) and delivers synchrotron radiation (X-rays) simultaneous to 16 experiments, each highly specialized for specific applications (Tomography, protein crystallography, lithography, etc. (psi.ch)
  • Recent years have seen the rapid development and expansion of electron crystallography, much of it highlighted at the IUCr Congress in Prague last year. (iucr.org)
  • For example, in biological physics these details are mainly obtained by x-ray crystallography of a crystallized version of the macromolecules. (cecam.org)
  • It is especially dedicated to crystallography of biological macromolecules. (esrf.fr)
  • The beams are directed into beamlines to support a wide range of scientific techniques, including protein crystallography. (lbl.gov)
  • On the Structural Biology Group beamlines one of the ultimate aims is that users can define protocols for experiments, click 'go' and let the experiments run by themselves", explains Gordon Leonard, head of the Structural Biology group at the ESRF. (lightsources.org)
  • ISPyB is a Laboratory Information Management System (LIMS) combining sample tracking and experiment reporting at synchrotron beamlines. (lu.se)
  • His main research goal has been to apply electron diffraction to structural problems in any field of crystallography. (iucr.org)
  • They also conducted high-throughput laboratory screening experiments, structural studies, and molecular dynamics simulations to learn how these potential inhibitors and the enzyme interact. (bnl.gov)
  • Structural studies using, for example, x-ray crystallography revealed how the candidate molecules fit together with the virus enzyme. (bnl.gov)
  • Recent developments within protein crystallography methods in combination with the opportunities offered by the new upcoming synchrotron sources such as the MAX IV 3 GeV storage ring, make new exciting ways of carrying out research within structural biology possible. (lu.se)
  • gP2S is a web application for the tracking of cryoEM experiments. (jove.com)
  • Once configured, the application allows one to accurately record metadata associated with negative stain and cryoEM experiments. (jove.com)
  • Being at a totally new class of facility we had to master many challenges that nobody had tackled before," says DESY scientist Anton Barty from the Center for Free-Electron Laser Science (CFEL), who led the team of about 125 researchers involved in the first experiments that were open to the whole scientific community. (bionity.com)
  • As announced in the January issue of the journal, Professor Xiaodong Zou (Stockholm University, Sweden) has been appointed as Main Editor of the electron crystallography section. (iucr.org)
  • Five new Co-editors will work with Professor Zou - Mauro Gemmi, Louisa Meshi, Peter Nellist, Jose Rodriguez and Junliang Sun - and will aim to attract articles reporting important electron crystallography results and breakthroughs to the journals. (iucr.org)
  • Since 2015 he has been the PI responsible for the Electron Crystallography research line of the Istituto Italiano di Tecnologia. (iucr.org)
  • Junliang Sun (Peking University, Beijing, China) works mainly on the development of structure characterization methods (electron crystallography and powder X-ray diffraction) and the synthesis of inorganic materials. (iucr.org)
  • An international collaboration led by DESY and consisting of over 120 researchers has announced the results of the first scientific experiments at Europe's new X-ray laser European XFEL . (bionity.com)
  • The groundbreaking work of the first team to use the European XFEL has paved the way for all users of the facility who greatly benefit from these pioneering experiments," emphasises European XFEL managing director Robert Feidenhans'l. (bionity.com)
  • Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals. (iucr.org)
  • Overall, our results illustrate the promise of serial methods for room-temperature crystallography, as well as future avant-garde crystallography experiments, for PTP1B and other proteins. (iucr.org)
  • With this idea in mind and to get as much information as possible from the samples available, the team has already adopted serial crystallography, a technique which involves taking diffraction data from many, sometimes hundreds or thousands, of crystals in order to assemble a complete dataset, piece by piece. (lightsources.org)
  • Artist's impression of the experiment: When the ultra-bright X-ray flashes (violet) hit the enzyme crystals in the water jet (blue), the recorded diffraction data allow to reconstruct the spatial structure of the enzyme (right). (bionity.com)
  • Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. (iucr.org)
  • Experiments with E. coli bacteria and eukaryotic cells revealed that the new peptide system is even suitable for transporting other peptides and proteins. (eurekalert.org)
  • When the structure of the interacting proteins is known, either from experiments or from modeling, several docking algorithms are available which attempt to predict the geometry of the complex. (cecam.org)
  • Today, there is an evolving need for more imaging methods and for biophysics that cannot be addressed with crystallisation and crystallography. (infn.it)
  • A method is called theoretical if it obtains its results from calcula- structure and function of metalloproteins with theoretical methods tions rather than from experiments. (lu.se)
  • New technical solutions for sample handling and presentation, data processing methods and more will be needed for these types of experiments, and these will also be presented and discussed at the workshop. (lu.se)
  • Other teams ran laboratory experiments testing those molecules' ability to inhibit the virus. (bnl.gov)
  • Once your experiment time slot (beamtime) at MAX IV has been scheduled, you log into DUO and create your Experiment Session. (lu.se)
  • X-rays are used in the experiment to probe the sample at high (up to atomic) resolution since the theoretical attainable resolution is related to the wavelength of the light used. (stanford.edu)
  • X-ray crystallography is the leading technique for determining the atomic structure of biological molecules. (ox.ac.uk)
  • Even first experiments on laser control in biological light-harvesting complexes have been performed [9]. (cecam.org)
  • Often atomic-level details are known from experiments in chemical and biological physics these days. (cecam.org)
  • Future experiments will explore whether the molecule can be developed into a new drug for treating COVID-19. (bnl.gov)
  • The key data challenges of complexity, velocity, and volume require a new data and computing infrastructure, including curation, detailed recording of processes acting on data, and advanced computing discovery techniques integrated into computational workflows to enable the autonomous experiments in the future. (bnl.gov)
  • In these video, we only illustrate one of the configuration steps, how to register a cryogenic sample holder, and then to illustrate in more detail how users can register experiments in gP2S. (jove.com)
  • In general, the BioSAXS experiment requires a highly purified sample (technically speaking, a monomodal and monodisperse sample). (stanford.edu)
  • To make the experiment work, we needed the nanofabrication technique at the Center for Nanoscale Materials to make the sample cell as well. (anl.gov)
  • Using X-ray crystallography to view Anf3's structure at an atomic resolution, the team saw that it was similar to a family of oxygen-reducing enzymes. (asbmb.org)
  • Examples of cutting edge diffuse scattering experiments and modern real structure modeling techniques are given. (chimia.ch)
  • For an accurate description of an experiment all modes which can be probed experimentally, e.g., via spectroscopic techniques, have to be treated explicitly. (cecam.org)
  • In facts, often limitations in the availability of high-quality samples for X-ray crystallography call for the use of lower-resolution techniques like cryo-EM, where a compelling reconstruction of the structure of the complex requires the combined use of advanced computational techniques. (cecam.org)
  • The pioneering work not only demonstrates that the new research facility can speed up experiments by more than an order of magnitude, it also reveals a previously unknown structure of an enzyme responsible for antibiotics resistance. (bionity.com)
  • Experiments show the structure and kinetics of a crucial COVID protein. (diamond.ac.uk)
  • X-ray crystallography offers detailed insights into protein structure. (iucr.org)
  • Allows setting up crystallisation drops with volumes as low as 50 nl protein + 50 nl reservoir and 1-3 protein samples per condition, thus 96-288 experiments can be done in one plate. (lu.se)
  • The Crystallography Open Database, an open-access collection of files for inorganic, organic, and metal-organic compounds and minerals, also is available through the same search system. (bnl.gov)
  • To conduct your experiment at MAX IV you must register as a MAX IV user. (lu.se)
  • The interplay between theory and experiment is an essential feature of the program. (lu.se)
  • Recent oxygen-17 magnetic relaxation dispersion (MRD) experiments have shown that the vast majority of water molecules in the protein hydration layer suffer a mere twofold dynamic retardation compared with bulk water. (lu.se)
  • This conference opens opportunities African Crystallographers to exchange ideas and collaborate in use of crystallography in advancing science and engineering is important in the development of sustainable economic growth in Africa. (chemistryviews.org)