Crystallin expression. Medical search. Frequent questions

It was found that CREB significantly suppressed expression of the αB-crystallin gene to sensitize CREB-expressing cells undergoing oxidative stress-induced apoptosis. (aging-us.com)
CREB knockdown via CRISPR/CAS9 technology led to upregulation of αB-crystallin and enhanced resistance against oxidative stress-induced apoptosis. (aging-us.com)
Moreover, overexpression of exogenous human αB-crystallin can restore the resistance against oxidative stress-induced apoptosis. (aging-us.com)
Together, our results demonstrate that CREB is an important transcription factor mediating stress response, and it promotes oxidative stress-induced apoptosis by suppressing αB-crystallin expression. (aging-us.com)
In UV models of ALCs and human lens epithelial cell lines, the expression levels of histone H3, cell apoptosis factors (Bax/Bcl-2, cleaved caspase-3), and inflammation factors (interleukin-6, tumor necrosis factor-α) were all up-regulated. (techscience.com)
Experiments are underway to systematically determine the role of αB-crystallin in conferring resistance of ovarian cancer cells to apoptosis. (bridgew.edu)
Conversely, wildtype αB-Crystallin and αB-Crystallin pseudophosphorylation mutants that alter its chaperone activity will be overexpressed in the OVCAR8 cells to determine the role of this protein in conferring resistance to WA-induced apoptosis. (bridgew.edu)
His Cell biology research incorporates elements of Heat shock protein, Alpha-Crystallin A Chain, Apoptosis, Lens and Genetically modified mouse. (research.com)

Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. (wikipedia.org)
Probing the Changes in Gene Expression Due to Α-Crystallin Mutations in Mouse Models of Hereditary Human Cataract. (wustl.edu)

Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. (nih.gov)
We sought to establish the effects of the loss of AND-34 expression in a mammalian organism. (molvis.org)
Improved mammalian vectors for high expression of G418 resistance. (riken.jp)

αA- and αB-crystallins are small heat shock proteins that bind thermodynamically destabilized proteins thereby inhibiting their aggregation. (nih.gov)
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. (caslab.com)

The present study was conducted to determine whether corneal cells differentially express genes for T4 regulation, keratan sulfate proteoglycan (KSPG) synthesis, crystallins, and endothelial cell ion transporters during transparency development and whether these expressions are altered when E9 embryos are treated with T4. (unboundmedicine.com)
The Vision Molecular Biology Laboratory studies regulation of specific gene expression during functional differentiation and development of the vertebrate eye. (uclahealth.org)
DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
Up-regulation of HSP90, a steroid receptor chaperone, in the AD CP may indicate abnormal hormone receptor expression in this secretory tissue. (iospress.com)
This review describes the regulation and function of the major stress proteins within mycobacteria such as the GroEL, GroES and DnaK homologues along with hspX (alpha-crystallin) and others. (caister.com)
His Molecular biology study combines topics from a wide range of disciplines, such as In vitro, Transgene, Cataracts, Mutant and Regulation of gene expression. (research.com)
Yoshida, T., Characterization of the chicken L-Maf, MafB and c-Maf in crystallin gene regulation and lens differentiation. (riken.jp)
Annexin A2: its molecular regulation and cellular expression in cancer development. (gzsys.org.cn)

Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. (wikipedia.org)
Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. (wikipedia.org)
α-crystallin is a member of the small heat shock protein family, which helps in maintaining and thickening of the cell wall and in providing stability to proteins that allow the bacteria to survive under harsher conditions 7 . (nature.com)
We observed lens abnormalities, including increased reflectance intensity, as a consequence of the interference with expression of these proteins. (nih.gov)
To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
CRYAA is one of the major lens proteins, accounting for 35% of all crystallins. (molvis.org)
Immunohistochemistry was performed to validate the expression of two upregulated (EpCAM, FADD) and two downregulated (NDRG1, αB-crystallin) proteins in 190 paraffin-embedded tissue samples. (biomedcentral.com)
Two upregulated (EpCAM, FADD) and two downregulated (NDRG1, αB-crystallin) proteins were associated with the progression of breast cancer. (biomedcentral.com)
In this study, expression of various stress proteins in the Alzheimer-diseased choroid plexus (CP) was assessed immunohistochemically. (iospress.com)
The bacterium responds with its own defense mechanisms that include the increased expression of stress proteins (also called heat shock proteins). (caister.com)

These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts. (nih.gov)

The aim of the present study is to determine whether histone H3, αA-crystallin (CRYAA), β-galactosidase (GLB1), and p53 are involved in the pathogenesis of ARC. (techscience.com)
A total of 99 anterior lens capsules (ALCs) of patients with ARC of various nuclear grades, ultraviolet models of ALCs, and two human lens epithelial cell lines (FHL-124 and SRA01/04) were used, and the expression of histone H3, CRYAA, GLB1, and p53 were detected by immunoblotting and reverse transcription and real time-quantitative polymerase chain reaction. (techscience.com)
Histone H3 and p53 in ALCs of patients with ARC were up-regulated in a grade-dependent manner, and the expression of CRYAA showed a positive association with histone H3, p53, and GLB1. (techscience.com)

Exogenous d-crystallin gene expression as probe for differentiation of teratocarcinoma stem cells. (riken.jp)

To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)
The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
Patterns of Crystallin Gene Expression in Differentiation State Specific Regions of the Embryonic Chicken Lens. (jefferson.edu)

HspX or α-crystallin, a 16 kDa protein, is one of the dominant antigens expressed during the latent stages of M. tuberculosis infection and under various other conditions like low oxygen tension, nutrient starvation or hypoxia 7 , 8 . (nature.com)

A more consistent and severe lens phenotype was evident in maternal/zygotic αA-crystallin mutants compared to those observed by morpholino knockdown. (nih.gov)
The penetrance of the lens phenotype was reduced by transgenic expression of rat αA-crystallin and its severity was attenuated by maternal αA-crystallin expression. (nih.gov)

To extend and confirm these findings, we generated an αA-crystallin knockout zebrafish line. (nih.gov)
His study explores the link between Crystallin and topics such as Knockout mouse that cross with problems in Anatomy. (research.com)

Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)
The scientist's investigation covers issues in Molecular biology, Cell biology, Crystallin, Heat shock protein and Alpha-Crystallin A Chain. (research.com)
His Alpha-Crystallin A Chain research integrates issues from Cell culture, Transfection, Gene expression, Nucleus and Wild type. (research.com)

Expression of Potassium-Dependent Sodium-Calcium Exchanger in the Murine Lens. (wustl.edu)
Expression of the murine alpha B-crystallin gene is not restricted to the lens. (research.com)

Transfection of 10 nM CTGF siRNA inhibited the TGF-β2-induced upregulation of CTGF, Col4α2, Col1α1, TGM2, and FN, whereas TSP-1 expression was not reduced. (arvojournals.org)

The small Hsps, αB-Crystallin and Hsp27, confer resistance to chemotherapy in several cancer cell lines, however, the role of WA on αB-Crystallin and Hsp27 expression in drug resistance remains unknown. (bridgew.edu)
Importantly, I found that αB-Crystallin and Hsp27 are constitutively expressed in the OVCAR8R cells, while OVCAR8 cells do not endogenously express these Hsps, suggestingthat overexpression of Hsps may confer resistance to chemotherapy and promote a more aggressive tumor type. (bridgew.edu)
Western blot analysis revealed higher HSP27 expression in FTLD than in controls, but without qualitative differences in banding patterns. (biomedcentral.com)
For example, a highly induced expression of HSP27 has been reported in the brains of aged persons and of patients with AD, being present in proliferating astrocytes, especially in AD in those areas rich in senile (Aβ) plaques. (biomedcentral.com)
Similarly, patients with other types of dementia (Parkinson-dementia complex, multi-infarct dementia and normal pressure hydrocephalus) showed less expression of HSP27 in reactive astrocytes than that in AD, but more than that in controls. (biomedcentral.com)

Expression of biomarkers modulating prostate cancer angiogenesis: differential expression of annexin II in prostate carcinomas from India and USA. (gzsys.org.cn)

TGF-β2 is capable of inducing the expression of ECM and basement membrane components in cultured ONH astrocytes via CTGF and upregulated TSP-1, a protein naturally involved in the activation of latent TGF-β. (arvojournals.org)

Small heat shock protein αB-crystallin is part of cell cycle dependent Golgi reorganization J Biol Chem 2004;279:43374-7. (uclahealth.org)

Two interrelated paradigms currently under investigation are: 1) Molecular basis of the differential expression of the gene for the small heat shock protein Alpha B-crystallin, a protein associated with with vision (e.g. in the ocular lens), oncogenesis (e.g. in breast cancer) and neural degeneration (e.g. in Alzheimer's disease). (uclahealth.org)
The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. (caslab.com)
We further identified αB-crystallin as an independent biomarker to predict lymph node metastasis and the outcome of breast cancer patients. (biomedcentral.com)
αB-crystallin, a stress-related protein that has recently been shown to be important for cell invasion and survival, was identified as a potential prognostic biomarker to predict the outcome of breast cancer patients. (biomedcentral.com)
αB-crystallin Expression in Breast Cancer is Associated with Brain Metastasis. (icr.ac.uk)

Finally, we provided first evidence that CREB directly regulates αB-crystallin gene. (aging-us.com)
Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)

These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. (nih.gov)
Introduction and expression of recombinant b-galactosidase genes in cleavage stage mouse embryos. (riken.jp)

Central to this investigation is an understanding of what gene products are required and expressed during a natural infection and how this expression changes over time (from initial colonization to causation of disease and spread of the pathogen to new hosts) and space (in different cells or tissues within the host). (cdc.gov)
mRNA expressions were determined by real-time PCR using cDNA synthesized from whole-cell RNA, cells expressing T4 receptor mRNAs assessed by in situ hybridization, and KS disaccharide sulfation measured by electrospray ionization tandem mass spectrometry (ESI-MS/MS). (unboundmedicine.com)
Bhat SP, Hale IL, Matsumoto B, Elghanayan D. Ectopic Expression of αB-crystallin in Chinese Hamster Ovary cells suggests a nuclear role for this protein. (uclahealth.org)
Following WA treatment for 24h, the endogenous level of αB-Crystallin is upregulated in a dose dependent manner in the cisplatin-resistant OVCAR8R cells, while the cisplatin-sensitive OVCAR8 cells upregulate αB-Crystallin and Hsp-27 at 2.5and 5.0μM WA. (bridgew.edu)
Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration. (gzsys.org.cn)

In this study, we performed a new bioinformatics analysis on microarray-based gene expression data obtained from the public domain to explore genes that were differentially induced by drugs between the group of capreomycin and PA-824 and the group of mainly the first-line TB drugs. (hindawi.com)

The laboratory is interested in (a) elucidating developmental and tissue-specific control of the heat shock promoter of the Alpha B-crystallin gene and (b) understanding the biological function of the Alpha B-crystallin protein. (uclahealth.org)
Demyelination during multiple sclerosis is associated with combined activation of microglia/macrophages by IFN-γ and alpha B-crystallin. (hersenbank.nl)

Zygotic DE-cadherin expression is required for processes of dynamic epithelial cell rearrangement in the Drosophila embryo. (riken.jp)

1991). "Human gamma-crystallin genes. (wikipedia.org)
Expression of 11β-hydroxysteroid dehydrogenase type 1 in the human hypothalamus. (hersenbank.nl)
Analysis of age-related changes in gene expression in human microglia. (hersenbank.nl)
Clinical significance of annexin II expression in human non-small cell lung cancer. (gzsys.org.cn)

Real-time PCR, used to determine the expression patterns of each of the sHsps in different developmental stages of codling moth revealed the presence of transcripts in all stages tested. (usda.gov)
Abnormal patterns of stress protein expression are found in the cerebral cortex and hippocampus of Alzheimer (AD) subjects. (iospress.com)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. (atlasantibodies.com)
Protein expression of protein kinase B, calcium/calmodulin-dependent protein kinase II, and phospholamban was measured by Western immunoblotting. (silverchair.com)

In this study, a genetically stable, marker-free recombinant MVA expressing α-crystallin of M. tuberculosis (rMVA. (nature.com)

The effect of CTGF silencing on the TGF-β2-modulated expression of these genes was investigated by transfection of CTGF small interfering (si)RNA before TGF-β2 treatment. (arvojournals.org)

Developmentally dictated expression of heat shock factors and specific interaction of the αB-crystallin heat shock promoter with HSF4. (uclahealth.org)
Canonical heat shock element in the αB-crystallin gene shows tissue-specific and developmentally controlled interactions with heat shock factor. (uclahealth.org)
A degenerate oligonucleotide primer, designed against the conserved a-crystallin domain, was used in 3' RACE reactions to amplify transcripts encoding sHsps expressed in the codling moth cell line, Cp169, subjected to heat shock. (usda.gov)
Consistent with characteristics of other sHsps, expression of CpHsp transcripts were greatly enhanced when insects were subjected to heat shock. (usda.gov)

Here, we have examined the role of α-crystallins in the development of the vertebrate zebrafish lens. (nih.gov)
Role of the Drosophila EGF receptor in determination of the dorsoventral domains of escargot expression during primary neurogenesis. (riken.jp)
αB-Crystallin will be silenced by CRISPR-Cas9 in the OVCAR8R and clones will be selected to address the role of αB-Crystallin on WA-induced 4apoptosis. (bridgew.edu)
Expression of annexin II in gastric carcinoma and its role in gastric cancer metastasis. (gzsys.org.cn)

His Cell biology research is multidisciplinary, incorporating elements of CD28, Genetically modified mouse, Retinal and Cathepsin D. His Crystallin study combines topics in areas such as Mutant and Notch signaling pathway. (research.com)

Transparency and non-refractive functions of crystallins--a proposal Exp Eye Res 2004;79:809-16. (uclahealth.org)

Characterization of tissue Transglutaminase expression in active white and grey matter MS lesions. (hersenbank.nl)

Its importance has been documented in a number of physiologic and pathologic conditions including neuronal and muscle development, lymphocyte activation, cardiac hypertrophy, switching of skeletal muscle fiber type, and expression of ion channels. (pancreapedia.org)

TGF-β2 treatment upregulated the expression of Col1α1, Col4α2, FN, CTGF, TGM2, and TSP-1 mRNA and protein in cultured astrocytes. (arvojournals.org)

Upregulated expression of annexin II is a prognostic marker for patients with gastric cancer. (gzsys.org.cn)

Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. (wikipedia.org)

The addition of Yoda1, a Piezo1 activator, induced an increase in transglutaminase 2 (TGM2) mRNA expression and activity through the extra-cellular signal-regulated kinase (ERK) 1/2 and c-Jun-NH2-terminal kinase1/2 pathways. (bvsalud.org)

Two of these methods, signature-tagged mutagenesis and in vivo expression technology, do not directly measure gene expression and do not allow true genomic-scale analysis, but they have been devised to identify genes necessary for pathogens during real infections. (cdc.gov)
A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
In ex vivo lenses, Yoda1 treatment induced Î³-crystallin cross-linking via TMG2 activation. (bvsalud.org)

However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
SUMO2 also interfered with the interaction between Sp1 and the coactivator, p300, and recruited a repressor, Sp3 to β-crystallin gene promoters, to negatively regulate their expression. (nebraska.edu)

Mechanistically, specificity protein 1 (Sp1), a major transcription factor that controls expression of lens-specific genes such as β-crystallins, was positively regulated by SUMO1 but negatively regulated by SUMO2. (nebraska.edu)

Anatomy2

Organisms3

Diseases1

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes2

Information Science2

Apoptosis8

Cataract2

Mammalian3

Small heat shock pr2

Regulation8

Proteins10

Cataracts1

CRYAA3

Exogenous1

Differentiation4

HspX1

Phenotype2

Knockout2

Chain3

Murine2

Upregulation1

Hsp275

Angiogenesis1

Basement membrane1

Biol1

Breast cancer5

Regulates2

Embryos2

Cells5

Microarray1

Alpha2

Epithelial cell1

Human4

Patterns2

Protein Expression2

Stable1

Transfection1

Heat4

Role4

Genetically1

Transparency1

Tissue1

Development1

Astrocytes1

Prognostic1

Peptide1

MRNA1

Vivo3

Interaction2

Transcription factor1