• To conduct the study, the plant extracts in concentrations of 1%, 0.5% and 0.25% and antibiotic controls were each tested on growing as well as non-growing B. burgdorferi cultures. (lymedisease.org)
  • In the search for antimalarials from ethnobotanical origin, plant extracts are chemically fractionated and biological tests guide the isolation of pure active compounds. (biomedcentral.com)
  • Table 1 Compilation of data from the literature on the anti-plasmodial effects of plant extracts and their fractionated active compounds. (biomedcentral.com)
  • Ethnobotanical plant extracts, including Ghanaian quinine and Japanese knotweed, show strong activity against B.burgdorferi, outperforming current antibiotics for the treatment of Lyme disease. (neurosciencenews.com)
  • The researchers show that plant extracts from black walnut, cat's claw, sweet wormwood, Mediterranean rockrose, and Chinese skullcap had strong activity against B. burgdorferi, outperforming both tested antibiotics. (neurosciencenews.com)
  • Therefore, there is a new approach to reduce the spread of pathogenic microbes by using alternative and natural methods such as using essential oils or plant extracts 5 . (microbiologyjournal.org)
  • Methods: The cytotoxicity of the plant extracts was determined with the AlamarBlue cell viability assay using the human glioblastoma cell line U87.CD4.CXCR4. (bvsalud.org)
  • Using regression analysis, the half maximal cytotoxic concentration (CC50) of the plant extracts were calculated from exponential curve fits, since they provided the highest coefficient of determination, R2. (bvsalud.org)
  • However, the cytotoxicity of most of the plant extracts (15/22) was low to moderate (CC50 = 21-200 µg/ml). (bvsalud.org)
  • Of these products, the Cryptolepis sanguinolenta extract caused complete eradication, while doxycycline and cefuroxime and other active herbs did not. (lymedisease.org)
  • Andrographis paniculata extract (standardized to 10% andrographolides) - 1065 mg, Cryptolepis sanguinolenta extract (10:1) - 426 mg, Houttuynia cordata extract (10:1) - 390 mg, Glycyrrhiza glabra extract (standardized to 26% glycyrrhizic acid) - 69 mg. (onlyx.com)
  • In subculture studies, only 1% Cryptolepis sanguinolenta extract caused complete eradication, while doxycycline and cefuroxime and other active herbs could not eradicate B. burgdorferi stationary phase cells as many spirochetes were visible after 21-day subculture. (s4me.info)
  • This study is believed to be the first time this extract has been documented to have a potential impact on B. burgdorferi , and additional laboratory and clincial studies should be conducted to investigate the potential role Cryptolepis sanguinolenta could play in the treatment of Lyme disease. (lymedisease.org)
  • The study found that seven of these natural product extracts at 0.25%-0.5% had better activity against the stationary phase B. burgdorferi culture than the control antibiotics doxycycline and cefuroxime, both of which are commonly used to treat Lyme disease. (lymedisease.org)
  • This study provides the first convincing evidence that some of the herbs used by patients such as Cryptolepis, black walnut, sweet wormwood, cat's claw, and Japanese knotweed have potent activity against Lyme disease bacteria, especially the dormant persister forms, which are not killed by the current Lyme antibiotics," says Dr Ying Zhang from the Johns Hopkins Bloomberg School of Public Health. (neurosciencenews.com)
  • What can Cryptolepis extract do for Lyme disease? (runtheyear2016.com)
  • Cryptolepis extract has proved to have antibacterial activity against broad spectrum bacteria such as Borrelia burgdorferi, which causes Lyme disease. (runtheyear2016.com)
  • Cryptolepis benefits include support for a wide range of diseases, including malaria, Lyme disease, and bacterial infections. (runtheyear2016.com)
  • A study published in the European Journal of Microbiology and Immunology discovered that stevia extracts are more effective in killing Lyme disease bacterium in the lab than the standard antibiotics. (lymetalk.net)
  • Scientific research has proven that several herbs and their extracts can successfully fight the spirochete bacteria that cause lyme disease. (plantmedicines.org)
  • The roots of cryptolepis, also known as nibima, kadze, gangamau, Ghanaian quinine and yellow-dye root (Cryptolepis sanguinolenta) have been used in Ghanaian traditional medicine for treatment of malaria for many generations. (runtheyear2016.com)
  • In a new study published in Frontiers in Medicine , researchers from the Johns Hopkins Bloomberg School of Public Health, with colleagues at the California Center for Functional Medicine and Focus Health, surveyed the power of 14 plant-based extracts to kill Borrelia burgdorferi, compared to the currently used Lyme antibiotics doxycycline and cefuroxime. (neurosciencenews.com)
  • Among them, 7 natural product extracts at 1% were found to have good activity against the stationary phase B. burgdorferi culture compared to the control antibiotics doxycycline and cefuroxime. (s4me.info)
  • grapefruit seed extract 250 mg 1 pill 2 times a day. (lymetalk.net)
  • Grapefruit seed extract is better tolerated than both tinidazole and metronidazole and supports killing of Lyme cysts. (lymetalk.net)
  • Take 1 tablespoon of Grapefruit seed extract, 2 times a day. (herbpathy.com)
  • Is fresh grapefruit as effective as Grapefruit Seed Extract? (herbpathy.com)
  • Further, Cryptolepis sanguinolenta and Polygonum cuspidatum (Japanese knotweed) showed strong activity against both growing B. burgdorferi (MIC=0.03%-0.06% and 0.25%-0.5% respectively) and non-growing stationary phase B. burgdorferi . (lymedisease.org)
  • These active botanicals include Cryptolepis sanguinolenta, Juglans nigra (Black walnut), Polygonum cuspidatum (Japanese knotweed), Artemisia annua (Sweet wormwood), Uncaria tomentosa (Cat's claw), Cistus incanus , and Scutellaria baicalensis (Chinese skullcap). (s4me.info)
  • Cryptolepis/cryptolepine is anti-inflammatory and cytotoxic with the inhibition of COX-2 and NF-kB as principal mechanisms. (runtheyear2016.com)
  • Different concentrations of crude methanolic extracts of seaweeds showed potential antimicrobial activity by well diffusion method. (google.com)
  • Ideally, the extracts are then tested against erythrocytic stages of Plasmodium falciparum in vitro to validate anti-plasmodial activity. (biomedcentral.com)
  • The researchers tested these extracts' effectiveness in vitro (outside of a living organism) against the free-swimming "planktonic" form of the bacterium as well as against microcolonies. (neurosciencenews.com)
  • AIM OF THE STUDY: To determine the ex vivo and in vitro antiplasmodial activities of the extracts of the roots, stem bark and leaves of Caesalpinia decapetala. (bvsalud.org)
  • In vitro Analysis of Antioxidant, Antimicrobial and Antiproliferative Activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata Extracts. (google.com)
  • The failure to isolate active constituents from active extracts may be due to the lability/instability of the active compounds that are degraded during the extraction process. (biomedcentral.com)
  • Constituents will be continually extracted with each brew. (diseasesolutions.net)
  • All constituents in this formula are extracted using Distilled Water and Organic Cane Alcohol (Gluten Free) handmade in small batches, blended and packaged at our facility in Maine. (sustenanceherbs.com)
  • An extract from the root is traditionally used in West Africa for malaria. (wikipedia.org)
  • This extract has been used for the treatment of malaria as well as the tick-borne infection Babesia , a malaria-like parasite. (lymedisease.org)
  • What are the benefits of Cryptolepis sanguinolenta for malaria? (runtheyear2016.com)
  • Cryptolepis sanguinolenta has proved to contain anti-parasitic activities against Plasmodium falciparum, a parasite that causes malaria. (runtheyear2016.com)
  • The golden Cryptolepis sanguinolenta we receive from Cameroon is the same species as that from Ghana but the roots are rouge tinted. (woodlandessence.com)
  • Depending on which roots are used to prepare our extract or glycerite, you will notice either a bright yellow-gold or rouge- gold color. (woodlandessence.com)
  • What are the roots of Cryptolepis used for? (runtheyear2016.com)
  • METHODOLOGY: The roots, stem bark and leaves of Caesalpinia decapetala (Roth) Alston (Caesalpiniaceae) were collected and air-dried under a shade then extracted consecutively with dichloromethane and methanol (1:1 (v/v) (4 × 0.8 L). The extracts were tested for antiplasmodial activities against four strains of Plasmodium falciparum (W2, DD2, 3D7, and D6) and fresh P. falciparum field isolates using the SYBR green I assay. (bvsalud.org)
  • Similarly, the roots extract showed an IC50 value of 6.49 µg/mL when tested against field isolates ex vivo. (bvsalud.org)
  • The roots extract showed the highest antiplasmodial activities among the samples when tested against W2 (IC50 = 6.12 µg/mL), DD2 (IC50 = 8.17 µg/mL), and D6 (IC50 = 16.02 µg/mL) strains of P. falciparum whereas the leaves showed the highest activity (IC50 = 9.3 µg/mL) when tested against the 3D7 strain of P. falciparum. (bvsalud.org)
  • The mouse treated with 2000 mg/kg of the roots extracts regained weight by day 12 of the observation period. (bvsalud.org)
  • Antioxidant properties of rosemary extracts vary due to genetic and growth conditions, region and geographical origin, climatic conditions, extraction process, main plant quality and date of harvest 27 . (microbiologyjournal.org)
  • The methanolic extracts of seaweeds Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata possess high total phenolic content and shows a good free radical scavenging activity and hence are proven to have better antioxidant activity and they might be good candidates for further investigations in order to develop potential anticancer drugs. (google.com)
  • Sometimes, the loss of activity is due to the fact that the compounds display their activity only when they interact in the crude extract. (biomedcentral.com)
  • In order to evaluate such interactions, mostly synergistic, it is necessary to know the inhibitory activity of the crude extract and the purified fractions (i.e., their IC 50 values) and the yields of extraction of the purified compounds to allow calculation of their absolute quantitative prevalence in the extract. (biomedcentral.com)
  • To determine the quantitative contribution of the pure compounds to the activity of a crude extract, data from the literature were compiled selecting those publications in which the activities of the crude extracts and of the purified compounds (and their yields) were reported. (biomedcentral.com)
  • Taking for example the case of the crude extract of Alstonia macrophylla and one of the most active compounds, macrocarpamine: one obtains a yield for macrocarpamine of 0.95 % and it is straightforward to calculate that it is present in the extract at 0.054 mg at the IC 50 of the extract. (biomedcentral.com)
  • Herbal compounds extracted in an organic alcohol base. (diseasesolutions.net)
  • Ingredients: Dried Cryptolepis root (from Africa), certified organic non-GMO cane alcohol, organic apple cider vinegar and pure Adirondack water. (woodlandessence.com)
  • Cryptolepis root contains Neocryptolepine as the main bioactive compound along with Biscryptolepine, a secondary component. (woodlandessence.com)
  • Paeonia lactiflora (White Peony Root) extract may provide support for common neurodegenerative diseases such as Parkinson's disease. (lindenbotanicals.com)
  • Bioactive components were extracted by using various solvents. (google.com)
  • Cryptolepis sanguinolenta is a species of flowering plant in the family Apocynaceae. (wikipedia.org)
  • o tinctures, powders, or dried extracts? (onlyx.com)
  • NutriFlair D-Mannose Supplement, 120 Vegan Capsules Delivers 1200 mg of a combination of DMannose, Cranberry, Dandelion Extract, per Serving of 2 Capsules. (florenmolamarts.com)
  • Extracts of grapefruit seeds, green chiretta, ashwagandha, candy leaf (also known as stevia), fuller's teasel, and Japanese teasel had little or no effect, and neither did the chemicals colloidal silver, monoglyceride monolaurin, or antimicrobial peptide LL37 from human immune cells. (neurosciencenews.com)
  • ️ Ultra Potent Cleanser: The NutriFlair D Mannose Pills features powerful ingredients such as D-Mannose, Cranberry Juice Powder (from fruit), and Dandelion Extract, proven to promote bladder wellness. (florenmolamarts.com)
  • Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)
  • This is the case of hundreds of thousands of purified fractions of natural extracts that have been evaluated by cell-based inhibition tests. (biomedcentral.com)
  • To establish the responsibility of isolated active compound(s) to the whole antiplasmodial activity of a crude extract, the literature in this field was scanned and results were analysed quantitatively to find the contribution of the pure compound to the activity of the whole extract. (biomedcentral.com)
  • It was found that, generally, the activity of isolated molecules could not account on their own for the activity of the crude extract. (biomedcentral.com)
  • Classically, when biological tests identify significant activity, crude extracts are submitted to a bioguided fractionation procedure, aiming to isolate the active compound(s). (biomedcentral.com)
  • Unfortunately, in most cases, when plants are extracted and fractionated, the activity of the crude extract is not determined and the yields are not reported or not determined altogether. (biomedcentral.com)
  • RESULTS: The leaves and stem bark extracts showed good antiplasmodial activities with IC50 values of 4.54 and 4.86 µg/mL, respectively, when tested against the fresh field isolates ex vivo. (bvsalud.org)
  • Fresh and dried rosemary leaves are used for their distinctive aroma in food or herbal tea cooking, while rosemary extracts are commonly used as natural antioxidants that help a person's healthy life 25 . (microbiologyjournal.org)
  • Higher activity of ethanolic extract was found against S. aureus (2.4 cm) than E. coli (1.8 cm). (microbiologyjournal.org)
  • It was found that methanolic extracts elicited higher total phenolic content, higher percentage scavenging activity of nitric oxide, hydrogen peroxide, hydroxyl radicals, free radical scavenging (DPPH), FRAP (ferric reducing ability plasma) ability and reducing power. (google.com)
  • International Plant Names Index Cryptolepis sanguinolenta in West African plants - A Photo Guide. (wikipedia.org)
  • Deficiencies in endocannabinoids can be balanced interim with extracts from cannabis plants which contain phytocannabinoids. (hempistani.com)
  • The EC 50 is the IC 50 of the isolated compound and "x" is the yield-dependent calculated concentration of the compound at the IC 50 of the extract. (biomedcentral.com)
  • Frequently, many promising extracts are discarded because the anti-plasmodial activity disappears along the fractionation process. (biomedcentral.com)
  • To calculate the contribution of the pure active compound to the activity of the extract, the respective IC 50 values and the yield of the purified compound are used. (biomedcentral.com)
  • Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis. (google.com)
  • In addition, the repetitive element PCR (Rep-PCR) significantly showed that several genetic numbers of polymorphic bands were observed in S. aureus and E. coli treated bacteria with leaves extracts and not observed in the control. (microbiologyjournal.org)
  • These results indicate that these extracts have a genotoxicity effect on the two bacterial genomes. (microbiologyjournal.org)
  • Results: The ethanol extracts of W. ugandensis (CC50 = 7.6 µg/ml) and A. coriaria (CC50 = 1.5 µg/ml) as well as the DMSO-reconstituted extracts of W. ugandensis (CC50 = 6.4 µg/ml) and A. coriria (CC50 = (bvsalud.org)
  • No mortality was observed for the mice treated with 2000 mg/kg of the leaves and stem bark extracts. (bvsalud.org)
  • E. compressa extracts alleviated the IgE levels raised against ovalbumin and otherallergens in mice. (google.com)
  • There have been various anecdotal reports on the use of herbal extracts for treating patients with persisting symptoms with varying degree of improvements. (s4me.info)