• We examined the effect of crotoxin, the neurotoxic complex from the venom of the South American rattlesnake Crotalus durissus terrificus, on the uptake of ³H-choline in minces of smooth muscle myenteric plexus from guinea pig ileum. (scielo.org)
  • Venom phospholipase A2 (PLA2) enzymes share similarity in structure and catalytic function with mammalian enzymes. (fapesp.br)
  • Crotalus durissus terrificus snake venom and its major toxin, crotoxin or type II PLA2 (CB) subunit of this toxin, modulates immune and inflammatory responses, interfering with the activity of leukocytes. (fapesp.br)
  • Higher concentrations of crotoxin or more extensive incubation times with this toxin resulted in elevation of LDH activity detected in the assay supernatant. (scielo.org)
  • The inhibitory effect of crotoxin on ³H-choline uptake seems to be associated with its phospholipase activity since the equimolar substitution of Sr2+ for Ca2+ in the incubation medium or the modification of the toxin with p-bromophenacyl bromide substantially decreased this effect. (scielo.org)
  • Moreover, this toxin also up-regulated protein expression of COX-2 isoform, whereas an inhibitor of phospholipases A2 enzyme activity, abrogated this effect. (fapesp.br)
  • Phospholipases attack cell and mitochondrial membranes destroying nerve and muscle function. (scienceblogs.com)
  • Secreted phospholipases A 2 (sPLA 2 s) participate in a very broad spectrum of biological processes through their enzymatic activity and as ligands for membrane and soluble receptors. (ijbs.com)
  • We suggest that this inhibition could explain, at least in part, the blocking effect of crotoxin on neurotransmission. (scielo.org)
  • 6. Biochemical, functional, structural and phylogenetic studies on Intercro, a new isoform phospholipase A2 from Crotalus durissus terrificus snake venom. (nih.gov)
  • 7. Role of crotoxin, a phospholipase A2 isolated from Crotalus durissus terrificus snake venom, on inflammatory and immune reactions. (nih.gov)
  • This paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA 2 ) from Crotalus durissus terrificus , which is the main toxin involved in the pharmacological effects of this snake venom. (hindawi.com)
  • Several recent studies have shown that snake venom sPLA 2 s present a mechanism of action that is very similar to that of human sPLA 2 s [ 4 , 5 ], and some secretory phospholipase A 2 s purified from humans can induce pharmacological events similar to those of snake venom phospholipase A 2 [ 6 ]. (hindawi.com)
  • Thus, there is great interest in using snake venom sPLA 2 s as molecular target model to evaluate and investigate for natural compounds that potentially inhibit the activities of phospholipase A 2 homologous molecules in other organisms [ 7 - 9 ]. (hindawi.com)
  • 4. A comparative study of biological activities of crotoxin and CB fraction of venoms from Crotalus durissus terrificus, Crotalus durissus cascavella and Crotalus durissus collilineatus. (nih.gov)
  • 8. Preliminary X-ray crystallographic studies of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom. (nih.gov)
  • 9. Crystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venom. (nih.gov)
  • 11. Antiplasmodial effect of the venom of Crotalus durissus cumanensis, crotoxin complex and Crotoxin B. (nih.gov)
  • 13. Insights into the role of oligomeric state on the biological activities of crotoxin: crystal structure of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom. (nih.gov)
  • 14. Insights on the structure of native CNF, an endogenous phospholipase A2 inhibitor from Crotalus durissus terrificus, the South American rattlesnake. (nih.gov)
  • 16. Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom. (nih.gov)
  • 17. The origin of the diversity of crotoxin isoforms in the venom of Crotalus durissus terrificus. (nih.gov)
  • This technique was used to assemble a partial venom gland transcriptome for the Middle American Rattlesnake (Crotalus simus tzabcan) by amplifying sequences for phospholipases A2, serine proteases, C-lectins, and metalloproteinases from within venom. (nih.gov)
  • 15. Crotoxin: novel activities for a classic beta-neurotoxin. (nih.gov)