Cross reactions of monoclonal antibodies. Medical search. Frequent questions

The AGID test can detect antibodies that have persisted for years in BTV-exposed animals and can cross-react with related orbiviruses, thereby producing high numbers of false negatives (low sensitivity and specificity). (purdue.edu)
The next stage is a rapid primary screening process, which identifies and selects only those hybridomas that produce antibodies of appropriate specificity. (wikipedia.org)
The specificity for and function of monoclonal antibodies against Tritrichomonas foetus were characterized. (docksci.com)
In order to overcome serological cross-reactions among orbivirus serogroups, which can hinder the accurate diagnosis of bluetongue virus (BTV) infection of livestock, a blocking ELISA (B-ELISA) incorporating a monoclonal antibody (20E9B7G2) with specificity for the BTV serogroup was developed. (ox.ac.uk)
The sensitivity and specificity of the assay was evaluated with a range of experimental and field sera and compared to a sensitive indirect ELISA (I-ELISA) for the detection of BTV-specific antibodies. (ox.ac.uk)
The specificity of the B-ELISA was absolute for antibodies to BTV, showing no cross-reaction with experimental antisera to serotypes of the closely related orbivirus causing epizootic haemorrhagic disease of deer. (ox.ac.uk)
Monoclonal and polyclonal antibodies were raised to the apoproteins of the chlorophyll a/b-binding peripheral light-harvesting complex of photosystem I (LHC I). Assays of polyclonal antibody specificity showed immunological cross-reaction between individual LHC I apoproteins and between the apoproteins of the LHC I and LHC II. (warwick.ac.uk)
The LHC I apoproteins were found to fall into two distinct immunological groupings which were supported by assays of monoclonal antibody specificity and by a comparison of their partial peptide maps. (warwick.ac.uk)
No cross-reaction and high specificity. (ballyabio.com)

The competitive ELISA (C-ELISA) has proven to be the best serologic test for BTV antibody detection. (purdue.edu)
Monoclonal antibody detection is used with ELISA to decrease the chance of cross-reaction. (purdue.edu)
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on goat IgG and with those common to other goat immunoglobulins. (jacksonimmuno.com)
The antibody has been tested by ELISA to ensure minimal cross-reaction with horse, human, mouse, rabbit and rat immunoglobulins, but it may cross-react with immunoglobulins from other species. (jacksonimmuno.com)
Supernatants were tested for antibody by an enzyme-linked immunosorbent assay (ELISA), as described below, with whole T. foetus as the antigen. (docksci.com)
Evaluation of a monoclonal antibody blocking ELISA for the detection of group-specific antibodies to bluetongue virus in experimental and field sera. (ox.ac.uk)
In particular, the B-ELISA detected a BTV-specific antibody response much earlier after infection that the I-ELISA, while still exhibiting full sensitivity to BTV antibody titres several months after infection. (ox.ac.uk)
This ELISA kit shows no cross-reactivity with other relevant proteins. (biovision.com)
Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
Using these antibodies acompetitive ELISA (enzyme-linked immunosorbent assay) wasdeveloped, and successfully used to enumerateNitrobacterin pure cultures as well as in activatedsludge. (avhandlingar.se)
Detected by radioimmunoassay technique, fragments exist for C-telopeptide of type 1 collagen (CTX, CrossLaps) and cross-linked N-terminal telopeptide of type 1 collagen by ELISA technique (NTX, Osteomark). (medscape.com)

Antigen and antibody testing for histoplasmosis may be negative in some patients with active infection. (empr.com)
This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
A type of white blood cell, the B cell, produces antibodies that bind to the injected antigen. (wikipedia.org)
Laboratory animals (mammals, e.g. mice) are first exposed to the antigen against which an antibody is to be generated. (wikipedia.org)
Small peptide and fragment antigens may not be good antigens-monoclonal antibody may not recognize the original antigen. (denovobiotech.com)
Chances of getting a better response to the antigen is increased- can try different animal sources as antibody produced recognizes different epitopes on the same antigen. (denovobiotech.com)
Non specific interaction with the antigen- considerable heterogeneity within the antibody pool. (denovobiotech.com)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. (wikidata.org)
A 250 kDa antigen implicated in the induction of protective immunity to Plasmodium chabaudi was examined with a panel of 11 monoclonal antibodies in cloned parasite lines. (ox.ac.uk)
Using cloned lines of P. falciparum and a second series of monoclonal antibodies, similar antigenic and structural diversity was evident in the equivalent antigen from the important human pathogen. (ox.ac.uk)
Serologic cross-reactivity of T11 target structure and lymphocyte function-associated antigen 3. (ox.ac.uk)
A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myeloma cells. (thecannabisadvisory.com)
Of the resulting antigen/antibody complexes, the T3 analogue/antibody complexes are bound to the streptavidin coated solid phase. (cdc.gov)

The study has identified valuable new reagents for studies of cattle and confirmed that most common cross-reactive MAb are to epitopes on integrins. (ox.ac.uk)
Antibodies directed at epitopes unique to or overexpressed on the cancer cells could be of therapeutic utility. (ias.ac.in)
The demonstration of epitopes cross-reactive with hsp 65 of mycobacteria in normal and, in higher quantity, in arthritic rat joints, suggests, together with our preliminary biochemical findings, that a recently identified mammalian counterpart to bacterial hsp 65 is both preferentially expressed in normal joints and subject to increased expression in arthritis of different aetiologies. (ox.ac.uk)
The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. (thermofisher.com)
The first two antibodies (TF3.8 and TF3.2) did not react with the surface of T. foetus, as determined by live-cell immunofluorescence, agglutination, and immobilization, whereas two other monoclonal antibodies (TF1.17 and TF1.15) did react with surface epitopes, as determined by these criteria. (docksci.com)
Neutralization potency of monoclonal antibodies recognizing dominant and subdominant epitopes on SARS-CoV-2 Spike is impacted by the B.1.1.7 variant. (ox.ac.uk)
RBD is the dominant target for neutralizing antibodies, and several neutralizing epitopes on RBD have been molecularly characterized. (ox.ac.uk)
100 monoclonal antibodies targeting epitopes on RBD, NTD, and S2 from SARS-CoV-2-infected individuals. (ox.ac.uk)
This work demonstrates that neutralizing antibodies targeting subdominant epitopes should be considered when investigating antigenic drift in emerging variants. (ox.ac.uk)

Four monoclonal antibodies generated by immunization of mice with live T. foetus were selected on the basis of enzyme-linked immunosorbent assay reactions. (docksci.com)
This study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) based on a monoclonal antibody specific to thermal stable-soluble protein (TSSP) in pork fat tissue and apply it to detect pork fat tissue in heat-processed (autoclave, steam, roast, and fry) beef meatballs. (kosfaj.org)

The production and characterization of monoclonal antibodies against toluene diisocyanate (TDI)-conjugated proteins. (cdc.gov)

Bluetongue is an orbivirus which cross-reacts with many antigenically related viruses including Palyam virus and the viruses that cause epizootic hemorrhagic disease of deer and African Horse sickness. (purdue.edu)
Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. (thermofisher.com)
Cross reacts with mouse and rat protein. (covalab.com)
Reacts with influenza A hemagglutinin H1 (A/Michigan/45/2015/H1N1) and H1(A/California/07/2009/H1N1), no or minimal cross reaction to other HA subtypes as tested. (eenzyme.com)
When the body reacts to a substance that is in no way innately harmful (an allergen), this is called an allergic or hypersensitivity reaction (Haith, 2005). (ceufast.com)
The hTSH binds to the immobilized monoclonal anti-hTSH on the solid phase while the goat anti-hTSH-alkaline phosphatase conjugate reacts with a different antigenic site on the hTSH. (cdc.gov)
During the incubation, free T3 in the sample reacts with the anti-T3 antibody. (cdc.gov)

This assay employs a monoclonal antibody specific for rat IL-6 coated on a 96-well plate. (biovision.com)
The overall assay time wasshortened and a lower detection limit could be obtained byusing the biosensor.The cross-reactivity of a number of bacterial strains andisolates were determined. (avhandlingar.se)
The Access HYPERsensitive hTSH assay has virtually no cross-reactivity with other peptide hormones. (cdc.gov)
The NTX assay employs a monoclonal antibody directed against a urinary pool of collagen cross-links (originating from a patient with Paget disease). (medscape.com)

Assays have been developed based on specific antibodies raised in an immune response against isolated collagen peptides containing cross-links. (medscape.com)
Urine from healthy individuals has produced a detectable reaction from these assays, and elevated turnover has produced large increases. (medscape.com)
HCV-specific antibodies were detected in 376 (67.3%) serum samples using third-gen- eration enzyme immunoassay and confirmatory immunoblot assays. (who.int)

We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
Thus, our results can establish the potential risk for autoimmunity and multi-system disorders with COVID-19 that may come from cross-reactivity between our own human tissues and this dreaded virus, and thus ensure that the badly-needed vaccines and treatments being developed for it are truly safe to use against this disease. (frontiersin.org)
While, naturally, the search for a successful vaccine and efficient treatment protocols are paramount, immunologists who focus on autoimmunity have been concerned whether the infection or even a newly developed vaccine itself can trigger autoimmunity via cross-reactivity. (frontiersin.org)
Cross-reactivity occurs when amino acid sequence homology exists between a pathogen and self-tissue proteins ( 1 ). (frontiersin.org)
In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
There are three important questions regarding the role of cross-reactivity with SARS-CoV-2. (frontiersin.org)
First, does cross-reactivity play a role in the multi-system disorders associated with SARS-CoV-2 infection? (frontiersin.org)
Second, how does cross-reactivity contribute to the pathophysiology of SARS-CoV-2-induced autoimmunity? (frontiersin.org)
We will begin with the first question of whether cross-reactivity can be involved in the multi-system response of COVID-19 infection. (frontiersin.org)
A fraction of COVID-19 convalescent individuals mount a potent antibody response to SARS-CoV-2 with cross-reactivity to SARS-CoV-1. (nih.gov)
We isolated and analyzed 126 monoclonal antibodies, many of which were sarbecovirus cross-reactive, with some displaying merbecovirus- and embecovirus-reactivity. (nih.gov)
Cross-reactivity with bovine cells of monoclonal antibodies submitted to the 6th International Workshop on Human Leukocyte Differentiation Antigens. (ox.ac.uk)
Twelve subpanels of monoclonal antibodies (MAb) included within the 6th International Workshop on Human Leukocyte Differentiation Antigens (6th HLDA) were assayed for reactivity with bovine peripheral blood leukocytes. (ox.ac.uk)
A monoclonal antibody reactive with the mycobacterial 65 kDa heat shock protein (ML 30) was investigated for reactivity with biopsies from normal rat joints and with inflamed joints due to adjuvant arthritis (AA) or collagen induced arthritis (CIA). (ox.ac.uk)
The peptide maps also revealed some apparently conserved structure which may have been responsible for the antigenic cross reactivity. (ox.ac.uk)
The frequency and the degree of cross-reactivity to other legumes are not known. (tno.nl)
Here we show that this functional cross-reactivity is most likely due to a structural homology of these molecules. (ox.ac.uk)
The immunological cross-reactivity between the polypeptides of the LHC I and LHC II were shown to be spurious. (warwick.ac.uk)
Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. (biovision.com)
These similarities result in varying degrees of cross-reactivity of different antisera. (cdc.gov)
This report describes generation of a recombinant Sudan virus and demonstrates its utility by quantifying antibody cross-reactivity between Ebola and Sudan virus glycoproteins after human infection or vaccination with a licensed Ebola virus vaccine. (cdc.gov)
This MoAb Showed no cross-reactivity with 12 other heterologous parasite antigens, including promastigotes of Leishmani. (bvsalud.org)
In 2002 and at that time to check for cross-reactivity. (cdc.gov)
It also confirms the tive samples, 12 negative samples and 16 presence of infection in patients with incon- with indeterminate results by confirma- clusive antibody reactivity. (who.int)

Since the antibodies in a well are produced by the same B cell, they will be directed towards the same epitope, and are thus monoclonal antibodies. (wikipedia.org)
The configuration of E Rosettes with sheep red blood cells and the expression of the epitope detected for the monoclonal antibody anti-CD2 was detected in mononuclear cells from 9 Cebus apella monkeys. (bvsalud.org)

Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). (wikipedia.org)
These antibody producing B-cells are then harvested from the mouse and, in turn, fused with immortal B cell cancer cells, a myeloma,[clarification needed] to produce a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and the longevity and reproductivity of the myeloma. (wikipedia.org)
The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which consists of genetically identical hybridomas which produce one antibody per culture (monoclonal) rather than mixtures of different antibodies (polyclonal). (wikipedia.org)
In contrast to polyclonal antibodies, which are mixtures of many different antibody molecules, the monoclonal antibodies produced by each hybridoma line are all chemically identical. (wikipedia.org)
Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal bovine serum) and produce antibodies. (wikipedia.org)
Hybridoma serves as an immortal source of monoclonal antibody. (denovobiotech.com)

In any case, the detection of BTV antibodies is poorly correlated with BTV viremia. (purdue.edu)
A biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently. (biovision.com)
In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. (nordicmubio.com)
An enzyme, conjugated tothe secondary antibody, catalyzes the formation of anelectroactive product, which can be amperometrically detected.Quantitative detection ofNitrobacterusing the immunosensor was compared to theELISA showing compatible results. (avhandlingar.se)
The former was utilized for detection of anti-HCV antibodies while the Before the introduction of screening of latter was used for HCV-RNA detection and blood donors for hepatitis C virus (HCV), subsequent genotyping/subtyping. (who.int)

Several isolated broadly neutralizing antibodies were effective against B.1.1.7, B.1.351, B.1.429, B.1.617, and B.1.617.2 variants and 19 prominent potential escape sites. (nih.gov)
However, infectious disease, baseline data are necessary to allow for 16 samples had detectable neutralizing antibodies against future public health assessment of its impact. (cdc.gov)

Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)

Other cross-reacting MAb either recognized antigens already defined in cattle or antigens not yet clustered in humans. (ox.ac.uk)

The product of a hybrid cell clone, P 3 W 80 , obtained as ascites fluid from mouse peritoneal cavity had high titres of anti-human chorionic gonadotropin antibodies e.g. 30 to 40% binding of 125 I-human chorionic gonadotropin at 10 7 dilution in a radioimmunoassay. (ias.ac.in)

A sample is added to a reaction vessel with goat anti-hTSH-alkaline phosphatase conjugate, buffered protein solution, and paramagnetic particles coated with immobilized mouse monoclonal anti-hTSH antibody. (cdc.gov)

We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
A monoclonal antibody to the mycobacterial 65 kDa heat shock protein (ML 30) binds to cells in normal and arthritic joints of rats. (ox.ac.uk)
A cross-reacting protein found in both coconut and hazelnut was identified as the cause. (perfect10nailstudio.co.uk)
It's possible to be allergic to an apple, but it has no pollen and so little protein content, any reaction may be too muted to even be detectable. (perfect10nailstudio.co.uk)
Iron vegan sprouted protein bars - allergic reaction? (perfect10nailstudio.co.uk)
Possibly having an adverser reaction to the whey protein or another ingredient in the bars- check out all the ingredients to see if you have had a problem with any of the other items. (perfect10nailstudio.co.uk)
Two people allergic to walnuts were found to react to coconuts due to a cross-reacting protein. (perfect10nailstudio.co.uk)
Hearing of allergic reactions from customers, RXBAR expanded a December recall of its protein bars that might have an undeclared peanut allergen. (perfect10nailstudio.co.uk)
Allergic reactions to properties inside of protein powder prohibit many people from taking certain kinds of supplements. (perfect10nailstudio.co.uk)
Protein is one major constituent of food that is known to trigger allergic reactions in pets. (perfect10nailstudio.co.uk)
As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). (nordicmubio.com)
This kit is specifically designed for the PAT bar protein expressed by the bar/pat gene in transgenic plants, and is developed by using monoclonal antibody preparation, immunochromatography and colloidal gold tracing techniques. (ballyabio.com)
Other mAbs cross-reacted with the above conjugates, methylene diphenyldiisocyanate- and hexamethylene diisocyanate protein conjugates. (cdc.gov)

In cases of a positive DAT, further investigation using specific monoclonal reagents to detect IgG or a complement (C3d) was carried out. (banglajol.info)

RPA-T4 blocks HIV binding and mixed lymphocyte reaction. (thermofisher.com)

A monoclonal antibody (Moab) 2C4 has been generated, which binds with cells of two androgenindependent prostate cancers, DU145 and PC3, and does not bind to peripheral blood leukocytes (PBLs) of healthy donors. (ias.ac.in)
Monoclonal Antibody AP3 Binds Galactomannan Antigens Displayed by the Pathogens Aspergillus flavus, A. fumigatus, and A. parasiticus. (olmdiagnostics.com)

Viruses or even bacteria can be allergens as they can trigger a reaction unrelated to their normal viral or bacterial influence in the body (Haith, 2005). (ceufast.com)
In addition, we found that some therapeutic monoclonal antibodies (REGN10987 plus REGN10933, COV2-2196 plus COV2-2130, and S309) and antiviral drugs (molnupiravir, nirmatrelvir and S-217622) can restrict viral infection in the respiratory organs of BA.2-infected hamsters. (nature.com)
One vial containing 420 mg of trastuzumab, a humanised IgG1 monoclonal antibody produced by mammalian (Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)
A broad antibody response was detected that included immunoglobulin G (IgG) and IgA subtypes with demonstrable Fc-mediated effector function targeting multiple viral antigens. (cdc.gov)

The antibody had heavy chain of IgG 1 and light chain of kappa type. (ias.ac.in)
This monoclonal antibody is specific to the lambda light chain of immunoglobulins and shows no cross-reaction with the kappa light chain or any of the five heavy chains. (teomics.com)

These properties are suggestive of the heterogeneity of the antigenic determinants in zona and emphasize the employment of appropriate bioassays for screening and selection of bioeffective antibodies. (ias.ac.in)

For blocking, normal serum (5% v/v) from the host species of the labeled secondary antibody is recommended. (jacksonimmuno.com)

These cells produce antibodies (a property of B cells) and are immortal (a property of myeloma cells). (wikipedia.org)
Can produce antibodies when needed. (denovobiotech.com)

The latter two monoclonal antibodies also mediated complementdependent killing of T. foetus and prevented of adherence of organisms to bovine vaginal epithelial cells. (docksci.com)

The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)

The cultures should be confirmed by microscopic identification of the characteristic intracytoplasmic inclusions, preferably by fluorescein-conjugated monoclonal antibodies specific for C. trachomatis. (cdc.gov)
that LTA and LPS activated specific reactions in SH-SY5Y cells by in a different way changing the expressions of iron uptake, BMS-777607 biological activity aswell as cytosolic and mitochondrial iron storage space proteins. (bioskinrevive.com)
This leads to a strong and specific agglutination without cross reaction. (generon-food-safety.com)
2 antibodies cross-reacted with the different parasite lines while 9 were specific for one line. (ox.ac.uk)
More specific antibodies are therefore needed. (olmdiagnostics.com)
NTD-specific antibodies formed two distinct groups: the first was highly potent against infectious virus, whereas the second was less potent and displayed glycan-dependant neutralization activity. (ox.ac.uk)
Mutations present in B.1.1.7 Spike frequently conferred neutralization resistance to NTD-specific antibodies. (ox.ac.uk)
The optimum working dilution of the antibody for any specific application should be established by titration. (nordicmubio.com)
screened together with and without specific template to find oligo combinations, which do not give false positive reactions. (9pdf.co)
The T-helper 1 responses are more specific, and the body develops antibodies to these allergens when the T-helper 1 system is activated. (ceufast.com)
The production of TDI-specific monoclonal antibodies (mAb) will allow the development of standardized immunoassays for exposure assessment. (cdc.gov)
The pyridinium compounds PYD and DPD, as well as hydroxypyridinium cross-links collagen, are among the more specific markers. (medscape.com)
ABSTRACT The seroprevalence of hepatitis C virus (HCV) specific antibodies and HCV genotypes distribution were studied among 559 Iraqi children with thalassaemia in receipt of repeated blood transfusions. (who.int)
They were tested for HCV-RNA positivity lence of hepatitis C virus specific antibodies and subsequent HCV-genotyping using an among children with thalassaemia in receipt advanced molecular method. (who.int)

Three of the four monoclonals prepared in a subsequent batch gave strong immunofluorescent reactions and had high titres as determined by enzyme immunoassay. (ias.ac.in)

Titers of 64 for IgG and 32 for IgM analysis, and a negative control was created by using an in patient serum specimens were considered evidence of irrelevant monoclonal mouse antibody. (cdc.gov)
We used this system to evaluate the neutralization activity of monoclonal and polyclonal antibodies, plasma from NiV-infected hamsters, and serum from human patients. (cdc.gov)

An autoimmune phenomenon has also been postulated to underlie guttate psoriasis because some streptococcal products and components have been found to cross-react with normal human epidermis. (medscape.com)
The antibody does not react with the heavy chain of goat IgG. (jacksonimmuno.com)
this antibody does not react with CLIP (aa 17-39 of ACTH). (teomics.com)

Since these functions are in vitro correlates of protection, the antigens recognized by these monoclonal antibodies may induce protective immunity. (docksci.com)

However, the use of monoclonal antibodies (mAbs) for the diagnosis of IA is often associated with false positives due to cross-reaction with bacterial polysaccharides. (olmdiagnostics.com)

Development and characterization of new monoclonal antibodies against porcine Interleukin-17A and Interferon-gamma. (usda.gov)
Monoclonal antibodies derived from ten hybrid cell clones, generated against porcine zona pellucida gave strong immunofluorescence with zona but the pattern varied from patchy, thin rim to heavy precipitation type of rim. (ias.ac.in)
Five of the 6 monoclonals studied prevented the binding of the porcine epididymal sperm to homologous oocytes in vitro , whereas the sixth one was partially effective. (ias.ac.in)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

No staining with ML 30 was seen in biopsies from inflammatory lesions due to delayed type hypersensitivity reactions in the skin of rats. (ox.ac.uk)

Monoclonal antibody TF3.8 recognized a predominant band at -155 kilodaltons, whereas TF3.2 reacted with several bands. (docksci.com)

The recent emergence of SARS-CoV-2 Omicron (B.1.1.529 lineage) variants possessing numerous mutations has raised concerns of decreased effectiveness of current vaccines, therapeutic monoclonal antibodies and antiviral drugs for COVID-19 against these variants 1 , 2 . (nature.com)
These findings suggest that the replication and pathogenicity of BA.2 is similar to that of BA.1 in rodents and that several therapeutic monoclonal antibodies and antiviral compounds are effective against Omicron BA.2 variants. (nature.com)
In addition, we evaluated the efficacy of therapeutic monoclonal antibodies and antiviral drugs for COVID-19 against BA.2 variants in vivo. (nature.com)

Triggers may induce an autoimmune reaction by mimicking molecular sequences in the epidermal basement membrane (molecular mimicry, as with drugs and possibly infections), by exposing or altering normally tolerated host antigens (as with physical triggers and certain disorders), or by other mechanisms. (msdmanuals.com)

We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
The myeloma cell line that is used in this process is selected for its ability to grow in tissue culture and for an absence of antibody synthesis. (wikipedia.org)
These mediators cause the common symptoms of an atopic reaction by causing vasodilation, capillary permeability, mucus production, smooth muscle spasm, eosinophil and T-infiltration into tissue as well as other inflammatory cells. (ceufast.com)

Mouse monoclonal antibody to CaMKIV. (bioskinrevive.com)
Goat anti-mouse antibody is used to immobilize the mouse anti-hTSH antibody. (cdc.gov)

The myeloma cells are selected beforehand to ensure they are not secreting antibody themselves and that they lack the hypoxanthine-guanine phosphoribosyltransferase (HGPRT) gene, making them sensitive to the HAT medium (see below). (wikipedia.org)

Allergic reactions to meals consumed outside the home are common and can be severe and sometimes fatal. (tno.nl)
Background: The use of lupine in food has been increasing during the last decade and allergic reactions to lupine have been reported, especially in peanut-allergic patients. (tno.nl)
related to the cross-allergic reactions. (perfect10nailstudio.co.uk)
The lack of nuts can keep those with nut allergies from various unpleasant allergic reactions. (perfect10nailstudio.co.uk)
Allergic reactions can vary depending on the cause - but symptoms include itching, sneezing, vomiting, diarrhoea or even an anaphalactic shock, which can be deadly. (perfect10nailstudio.co.uk)
Allergic Reactions. (perfect10nailstudio.co.uk)
Allergic reactions are mediated through the T-helper 2 responses which activate eosinophils and IgE production. (ceufast.com)
As mentioned above, there are four different categories into which allergic reactions lie. (ceufast.com)

We therefore highly recommend centrifuging (microcentrifuge) the reconstituted antibody before use and using the supernatant. (nordicmubio.com)

Increased chance for cross reaction - false positives. (denovobiotech.com)
Because the test strip is very sensitive, it is easy to cause cross-contamination and cause false positives. (ballyabio.com)

This antibody, along with the previously developed Moab 730, kills 100% of both DU145 and PC3 cells in the presence of complement and does not have a deleterious effect on PBLs of healthy males. (ias.ac.in)
Amonoclonal antibody (MoAb), designated TCY-3, against Trypanosoma cruzi was obtained from spleen cells of BALB/c mice immunized with crude soluble antigens of T. curzi trypomastigotes. (bvsalud.org)

Have choice of producing antibodies in different animals. (denovobiotech.com)

To determine the pathologic characteristics associated with R. africae infection at the cutaneous level, we analyzed skin biopsy specimens of patients with confirmed ATBF and compared the results with those from patients with Mediterranean spotted fever, the rickettsiosis caused by R. conorii , with respect to the pattern of inflammatory reaction. (cdc.gov)
with genital and anal specimens, false-positive test results occur because of cross-reaction with fecal flora. (cdc.gov)

Herein, by cross linking sodium alginate (SA), agar (AG) with Ti3C2Tx MXene and Zinc ions (Zn2+), a biosafe composite hydrogel (MSG-Zn2+) was developed for fast and efficient sterilization treatment. (bvsalud.org)
After reconstitution antibodies should be aliquoted and stored at -20 °C or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. (nordicmubio.com)

We evaluated the diagnostic value of immunohistochemical techniques by using a monoclonal antibody to R. africae . (cdc.gov)
Immunohistochemical stainings with the anti-hsp 65 antibody on paraffin sections from normal rat joints revealed a weak but exclusive staining of cells within the synovial lining. (ox.ac.uk)

Degradation products derived from the enzymatic hydrolysis of type 1 collagen, particularly peptides related to regions of cross-linking with PYD, are the best markers of bone resorption. (medscape.com)
The 2 categories of C-telopeptide methods are C-telopeptide of type 1 collagen (CTX) and type 1 collagen cross-linked C-telopeptide (ICTP). (medscape.com)

Guttate psoriasis is believed to result from an immune reaction triggered by a previous streptococcal infection in a genetically susceptible host. (medscape.com)
An allergy is "an acquired, abnormal immune response to a substance (allergen) that does not normally cause a reaction" (Thomas, 1997, pp. 66-67). (ceufast.com)
To find out the prevalence of alloantibodies and autoantibodies in repeatedly transfused patients so that serious hazards due to immune reaction may be avoided. (banglajol.info)
Total 370 patients who had received at least five units of transfusions were enrolled in this study but known patients of auto immune haemolytic anaemia, patients in whom antibody was previously detected and pregnant women were excluded from the study. (banglajol.info)

While difficult to perform, complement fixation is still used to determine BTV exposure status for export since it can detect shorter-lived antibodies. (purdue.edu)
One antibody, TF1.15, also killed in the absence of complement. (docksci.com)
NP antibodies are not associated with protection through neutralizing activity, but VRP vaccination results in NP antibodies possessing Fc-mediated antibody effector functions, such as complement activation (ADCD) and antibody-mediated cellular phagocytosis (ADCP). (cdc.gov)

This descriptive cross-sectional study was carried out in Department of Immunology, BIRDEM and Armed Forces Institute of Pathology (AFIP) during the period of July 2015 to June 2016. (banglajol.info)

However, because of BTV s wide pathogenic variability and the fact that cross-reaction may occur between other orbiviruses (especially EHD), a positive result on Bluetongue group test does not mean clinical signs seen were caused by BTV itself. (purdue.edu)

It has proven especially useful as an alternative to J2 to resolve cross-reactions and/or remove unwanted background, in those rare experimental setups where J2 did not provide satisfactory results. (nordicmubio.com)

The B cell that produces the desired antibodies can be cloned to produce many identical daughter clones. (wikipedia.org)

Furthermore, nonspecific reaction to the gelatin particles alon. (bvsalud.org)

Anatomy7

Organisms3

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes15

Disciplines and Occupations1

Information Science3

Health Care1

Specificity9

ELISA12

Antigen14

Epitopes9

Enzyme-linke2

Characterization of monoclonal antibodies1

Reacts7

Assay4

Assays3

Reactivity24

Epitope2

Hybridoma6

Detection5

Neutralizing Antibodies2

Antigens and Antibodies1

Humans1

Clone1

Conjugate1

Protein13

Reagents1

Lymphocyte1

Binds2

Viral4

Kappa2

Antigenic1

Secondary antibody1

Produce antibodies2

Bovine1

Differentiation1

Specific14

Immunoassay1

Serum2

React3

Immunity1

MAbs1

Porcine3

Affinity chromatography1

Delayed type hypersensitivity reac1

Predominant1

Therapeutic3

Induce1

Tissue3

Mouse2

Myeloma1

Allergic reactions8

Supernatant1

False positives2

Moab2

Animals1

Specimens2

Sodium2

Immunohistochemical2

Collagen2

Immune4

Complement3

Immunology1

Bluetongue1

Experimental1

Clones1

Particles1