• The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. (qiagen.com)
  • The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)
  • When tri(ethylene glycol) alone was used as the plasticizer, the cross-linking reaction did not occur as readily. (usda.gov)
  • During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. (qiagen.com)
  • The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues. (qiagen.com)
  • Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. (qiagen.com)
  • Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. (qiagen.com)
  • PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis. (qiagen.com)
  • IHC antigen retrieval reagent (citrate, pH 7.0) is used to recover antigens masked by fixation in cross linking fixatives such as formalin. (enzolifesciences.com)
  • Finally, amine reactive heterobifunctional cross-linkers can be used to introduce other functional groups for coupling ligands. (gbiosciences.com)
  • PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. (qiagen.com)
  • Knockout cells or tissue, as well as non-cross-linked cells, are good negative controls, while knockdown cells are not recommended. (abcam.com)
  • in vitro induction of apoptosis 3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining 4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. (biolegend.com)
  • immunochemical staining of dermal tissue and enzyme-linked immunoassays (ELISA). (cdc.gov)
  • In this work, we tested the procedures to crosslink functional groups in polar domains of humic acids by using carbodiimide coupling and analyzed to which extent influenced the modification their hydration properties and stability. (researchgate.net)
  • Alternatively, carbodiimide cross-linkers can be used to couple ligands to the amines via their carboxyl groups. (gbiosciences.com)
  • This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)
  • Fixatives that contain formaldehyde cross-link biomolecules and modify nucleic acids and proteins. (qiagen.com)
  • In order to reduce the impact of moisture on physical properties of articles based on zein, the use of various reagents to induce cross linking were evaluated on a torque rheometer. (usda.gov)
  • EOS9.1 antibody can induce apoptosis without cross-linking. (biolegend.com)
  • The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme's substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. (justia.com)
  • This additional cross-linking step prevents contamination of the purified antigen with antibody. (gbiosciences.com)
  • In order to develop zein fibers with improved physical properties and solvent resistance, formaldehyde was used as the cross-linking reagent before spinning. (usda.gov)
  • The formaldehyde cross-linked zein fabrics had double the tensile strength of control fibers. (usda.gov)
  • CLIP is an antibody-based technique used to study RNA-protein interactions related to RNA immunoprecipitation (RIP) but differs from RIP in the use of UV radiation to cross-link RNA-binding proteins to the RNA that they are bound to. (abcam.com)
  • Optional 4-thiouridine pre-incubation and UV-A crosslinking could be used for certain proteins. (abcam.com)
  • 4-thiouridine enhances crosslinking of some proteins. (abcam.com)
  • A reagent that forms a bond to its reaction partner (the electrophile) by donating both bonding electrons. (mcw.edu)
  • Our detailed protocol and tips for carrying out UV cross-linking and immunoprecipitation (CLIP). (abcam.com)
  • G-Biosciences Cross-Linking Immunoprecipitation kit contains all the reagents necessary to complete all aspects of immunoprecipitation, with the exception of labeling. (gbiosciences.com)
  • Based on our Classical Immunoprecipitation kit , the Cross-Linking Immunoprecipitation kit incorporates the homobifunctional cross-linker DSS to covalently couple the antibody to the Immobilized Protein A/G resin . (gbiosciences.com)
  • detection of gene probes, enzyme-linked immuno solid-phase assay, monoclonal antibody drug targeting and medical imaging. (justia.com)
  • TotalSeq™-D reagents are designed to profile protein expression at single cell level. (biolegend.com)
  • A hybridoma that produces MDI-protein specific IgM mAbs, which also has good cross-reactivity to hexamethylene diisocyanate conjugated protein, has recently been isolated and cloned. (cdc.gov)
  • To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
  • With suitable amounts of these reagents in the presence of water as a plasticizer, cross-linking occurred as evidenced by the very large increase in viscosity. (usda.gov)
  • Higher moisture uptake and water holding capacity were observed in humic acids, which were pre-wetted prior to crosslinking for at least 24 h. (researchgate.net)
  • For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. (biolegend.com)
  • The PowerPoint slide set and the webinar link can be found on our COCA Web page at emergency.cdc.gov/coca. (cdc.gov)
  • Mice homozygous for a targeted null mutation are viable and fertile but exhibit increased sensitivity to the DNA interstrand cross-linking agent mitomycin C. (jax.org)
  • Characterization of prepared derivatives showed that both methods lead to formation of crosslinked humic structures. (researchgate.net)
  • SDS-PAGE analysis clearly showed cross-linking has occurred. (usda.gov)
  • Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. (cdc.gov)
  • To view the papers in pdf format, click on the "PDF Full-text" link, and use the free Adobe Reader to open them. (mdpi.com)
  • Using of EDC resulted in lower degree of crosslinking, but better hydration properties. (researchgate.net)
  • The high- f junctions can provide load-bearing abilities similar to a covalent cross-link while retaining the ability to self-repair and concurrently confer stimuli-responsive properties arising from the high- f junction species. (aip.org)
  • Select check boxes to automatically save entries, or use 'save all' or 'save page' links above. (cdc.gov)
  • As both countries are considered to be a single, epidemiological block, cross-border activities continue to operate. (who.int)
  • Use the link below to share a full-text version of this article with your friends and colleagues. (wiley.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)