Cross linker for proteins cross linking reagents. Medical search. Frequent questions

Chemical cross-linking of proteins with mass spectrometry (XL-MS) is a rapidly developing structural biology technique able to provide valuable insight into protein conformations and interactions, even as they exist within their native cellular environment. (tuberculosistest.net)
Gaither C, Popp R, Zahedi RP, Borchers CH. Multiple reaction monitoring-mass spectrometry enables robust quantitation of plasma proteins regardless of whole blood processing delays that may occur in the clinic. (mcgill.ca)
Petrotchenko EV, Borchers CH. Protein Chemistry Combined with Mass Spectrometry for Protein Structure Determination. (mcgill.ca)
Cross-Linking Mass Spectrometry: A sneak peak into its world! (protrein.eu)
For this month's journal club, we therefore chose the review paper «Cross-linking mass spectrometry: methods and applications in structural, molecular, and systems biology» by F. O'Reilly and J. Rappsilber[1]. (protrein.eu)
Cross-linking mass spectrometry (CLMS) has emerged as a useful technique in structural biology research, complementing traditional approaches such as x-ray crystallography and electron microscopy. (protrein.eu)
General cross-linking mass spectrometry (CLMS) workflow. (protrein.eu)

SuperAmine 2 Microarray Substrate Slides contain covalent amine groups (2 x 10^13 per cm2) on linker arms that allow stable attachment of negatively-charged nucleic acids (cDNAs and oligos), proteins, small molecules, extracts and cells via electrostatic bonding with the positively charged amine groups. (arrayit.com)
In the cross-linking reaction, covalent bonds are formed between the reactive groups of the cross-linker and surface residues of proteins, peptides and/or nucleic acids (DNA and RNA). (protrein.eu)
This way, residues that are within a certain reach of each other can be linked, thereby providing information about tertiary structure as well as interactions between protein complexes and/or nucleic acids. (protrein.eu)
CLMS is not limited to studying the structure and interactions between proteins but can also be performed to gain knowledge about interactions between proteins and nucleic acids, however this blog post focuses specifically on the application and workflows involving proteins and peptides. (protrein.eu)

Anti-rabbit IgG, HRP-linked Antibody ( #7074 ). (cellsignal.com)
T opics that will be covered in the course  History of biosensor development, applications and requirements of biosensors and classification  Principles of molecular recognition and transduction signal acquisition Sourcesof Biological Recognition elements - enzymes/proteins, ssDNAs, antibody and Others Design considerations for use of recognition elements in biosensors Modeling of reactions for various biosensor applications‐ electrochemical, optical, piezoelectric, colorimetric, fluorometric and others. (slideshare.net)
However, over the years, we have expanded to other protein-related areas like protein, antibody services, and products. (lifetein.com)

[ 11 , 23 , 25 ] The highly repetitive protein subunit structure of viral capsids makes them well-equipped for induction of humoral immunity, due to efficient B-cell receptor (BcR) crosslinking and induction of neutralizing antibodies. (medscape.com)
Monoclonal antibodies against toluene diisocyanate haptenated proteins from vapor-exposed mice. (cdc.gov)
Monoclonal antibodies (MAbs) that recognize TDI bound proteins would be valuable tools/reagents, both in exposure monitoring and in TDI-induced asthma research. (cdc.gov)
These antibodies may be useful tools for the isolation of endogenous diisocyanate-modified proteins after natural or experimental exposures and for characterization of the toxicity of specific dNCOs. (cdc.gov)
The use of a fish protein, a non-mammalian protein, is that it eliminates or minimizes the interaction of antibodies raised in mammals. (gbiosciences.com)
The Blocking Agents Trial Packs are a set of four G-Biosciences proprietary blocking agents for immunoassays with one being a non-animal protein blocker and another protein free that minimizes the risk of non-specific binding of antibodies during the immunoassay. (gbiosciences.com)

2012 ). When using enzymes for digesting food proteins, an enzyme specific peptide composition, the so-called peptide fingerprint is formed. (springeropen.com)
To this end, the enzymes are chemically modified by adding crosslinkers like glutaraldehyde to the peptide chain (Walt and Agayn 1994 ). (springeropen.com)
For example, this brain-homing peptide can cross the blood-brain barrier. (lifetein.com)
Viral RNA annealing activities of human immunodeficiency virus type 1 nucleocapsid protein require only peptide domains outside the zinc fingers. (nih.gov)

Genipin is an excellent natural cross-linker for proteins, collagen, gelatin, and chitosan cross-linking. (wikipedia.org)
14. Interaction of specific platelet membrane proteins with collagen: evidence from chemical cross-linking. (nih.gov)

L-Cysteine hydrochloride salt is routinely used with Ellman's Reagent assays as a sulfhydryl standard. (gbiosciences.com)

Synthesis of a novel photoactivatable glucosylceramide cross-linker. (nih.gov)

In addition, a set of subgenomic mRNAs encoding structural and accessory proteins is produced by a sophisticated mechanism involving nested mRNA synthesis using the full-length genomic RNA as template. (iucr.org)
Associated with the cytoskeletal networks are a large variety of accessory proteins that serve to control their assembly, disassembly, dynamic properties, ability to cross link to each other both homotypically and heterotypically, and to various organelles. (nih.gov)

2. A membrane-impermeant, cleavable cross-linker. (nih.gov)
8. Cross-linking of intact erythrocyte membrane with a newly synthesized cleavable bifunctional reagent. (nih.gov)
11 The 4,5-dimethoxy-2-nitrobenzyl (DMNB)-containing linkers used in our previous study allowed optical MO uncaging, but cyclic cMOs could accommodate enzyme-cleavable linkers as well. (cdc.gov)
18 , 19 We surmised that cyclic cMOs containing an NfsB-cleavable linker could be used with analogous lines to knock down gene function in a tissue-specific manner (Figure 1 a). (cdc.gov)
Depending on the question you want answered and the workflow, the cross-linker may need to be cleavable in the mass spectrometer, be isotopically labeled or have properties that allow for enrichment. (protrein.eu)

In an activated form, they hydrolyze the protein substrate at specific sites and the released peptides can be absorbed. (springeropen.com)
The entirety of produced protein fragments and peptides is called proteolysome (Pimenta and Lebrun 2007 ). (springeropen.com)
This strategy relies on isotope encoded chemical cross-linkers that have the same molecular mass yet produce unique and specific isotope signatures upon fragmentation in the mass spectrometer which can be used for quantitative analysis of cross-linked peptides. (tuberculosistest.net)
Other peptides can cross the skin as transdermal peptides, target heart tissues as cardiac targeting peptides, and nuclear localization signal peptides. (lifetein.com)
After the cross-linking reactions, the proteins in the sample are digested by a protease (c) yielding a mix between cross-linked and linear peptides. (protrein.eu)
In some workflows, the cross-linked peptides are enriched (d) before data acquisition by MS/MS (e). (protrein.eu)
The last step is data analysis that aims at identifying cross-linked peptides (f). (protrein.eu)

3. Bis(sulfo-N-succinimidyl) [15N,2H16]doxyl-2-spiro-4'-pimelate, a stable isotope-substituted, membrane-impermeant bifunctional spin label for studies of the dynamics of membrane proteins: application to the anion-exchange channel in intact human erythrocytes. (nih.gov)
Bifunctional molecules that are designed to recruit E3 UBIQUITIN LIGASE to a specific target protein. (nih.gov)

15. Membrane-impermeant cross-linking reagents for structural and functional analyses of platelet membrane glycoproteins. (nih.gov)
Helical proteins designed with SEWING contain structural features absent from other de novo designed proteins and, in some cases, remain folded at more than 100°C," wrote the authors. (pharmaceuticalintelligence.com)
Essentially, the UNC scientists confirmed that the proteins they had synthesized contained the unique structural varieties that had been designed on the computer. (pharmaceuticalintelligence.com)
Conventional analytical techniques often cannot evaluate structural details of mAb microcrystals in the native suspension due to the presence of visible particles, relatively small crystal size, high protein concentration, and multicomponent nature of a liquid formulation. (tuberculosistest.net)
In circumstances where the complex topology is unknown, information concerning distance limits between surface residues of proteins that are known to be complex can be used with other structural research tools to aid modeling of the complex topology. (protrein.eu)

It has a low acute toxicity, with LD50 i.v. 382 mg/kg in mice, therefore, much less toxic than glutaraldehyde and many other commonly used synthetic cross-linking reagents. (wikipedia.org)
20. Cross-linking and coupling of rabbit muscle aldolase and glyceraldehyde-3-phosphate dehydrogenase by glutaraldehyde. (nih.gov)

The blue color of genipin is stabilized in milk due to the natural pH of milk In vitro experiments have shown that genipin blocks the action of the transporter uncoupling protein 2. (wikipedia.org)
5. In vitro properties and organ uptake of rat band 3 cross-linked erythrocytes. (nih.gov)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
The nucleocapsid (NC) protein NCp7 of human immunodeficiency virus type 1 (HIV-1) is important for encapsidation of the virus genome, RNA dimerization, and primer tRNA annealing in vitro. (nih.gov)

In industrial processes, protein hydrolysis is often carried out in batch processes and is mostly used in fermentation and enzyme based processes, like brewing, cheese manufacturing, meat tenderization or baking (Godfrey and Reichelt 1982 ). (springeropen.com)
Hydrolysis is conducted by an enzymatic or acidic breakdown of proteins (Tsugita and Scheffler 1982 ). (springeropen.com)

Conjugation of OVA was achieved by utilizing a heterobifunctional crosslinking reagent that reacted selectively with the cysteine thiol of the sHSP protein and lysine residues on OVA. (medscape.com)
12. Functions of extracellular lysine residues in the human erythrocyte anion transport protein. (nih.gov)
This type of cross-linker will create a highly dense mapping of distance restraints between residues on the protein surface. (protrein.eu)

Moreover, isotopic labeling and heteronuclear 13 C and 15 N spectroscopies have been utilized to identify the binding of caffeine in the pembrolizumab crystal lattice, providing molecular insights into the cocrystallization of the protein and ligand. (tuberculosistest.net)
With the ongoing progress in protein crystallography and NMR, structure-based ligand design has become more and more important. (combichem.net)
Proteolysis targeting chimera consist of a target protein ligand connected via a linker to an E3 ligand. (nih.gov)

High-resolution structures of the designed proteins CA01 and DA05R1 were solved by x-ray crystallography (2.2 angstrom resolution) and nuclear magnetic resonance, respectively, and there was excellent agreement with the design models. (pharmaceuticalintelligence.com)
During the five year initial phase of this effort the PSI pilot centers have made a significant progress in establishing high throughput protein structure determination pipelines using x-ray crystallography and NMR. (nih.gov)

HN - 2018(1987) MH - AAA Domain UI - D000074182 MN - G2.111.570.820.709.275.500.913 MS - An approximately 250 amino acid domain common to AAA ATPases and AAA Proteins. (nih.gov)
HN - 2018 FX - ATPases Associated with Diverse Cellular Activities MH - AAA Proteins UI - D000074582 MN - D8.811.277.40.13 MN - D12.776.157.25 MS - A large, highly conserved and functionally diverse superfamily of NTPases and nucleotide-binding proteins that are characterized by a conserved 200 to 250 amino acid nucleotide-binding and catalytic domain, the AAA+ module. (nih.gov)

SNAP-tag substrates are dyes, fluorophores, biotin, or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. (neb.com)

Simulation of the Protein-Shedding Kinetics of a Fully Vascularized Tumor. (stanford.edu)

Broadly defined, the cytoskeleton is a complex array of three major protein systems: microtubules, intermediate filaments and actin/microfilaments. (nih.gov)

Cysteine residue placement, as well as linker length within the heterobifunctional crosslinker, were screened to gain constructs that allowed optimal crosslinking of ovalbumin to the cage. (medscape.com)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)

1-Fluoro-2,4-dinitrobenzene [DNFB], commonly known as Sanger's reagent is a chemical amino acid modifier that reacts with N-terminal amino acid of polypeptides. (gbiosciences.com)

To facilitate an exchange of developments and advancements among participants in the PSI, the National Institute of General Medical Sciences (NIGMS) has organized annual workshops on gene cloning, protein expression, purification, protein crystallization and rapid NMR structure determination. (nih.gov)

The NHS ester group of Sulfo NHS Acetate reacts with primary amines of protein in non-amine containing buffers at pH 7-9. (gbiosciences.com)
FirstChoice™ Blocking Buffers contain a proprietary protein formulation that offers greater versatility and lack of cross-reactivity. (gbiosciences.com)

A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins. (deshaieslab.com)
They promote association of E3 with specific target proteins tagged for degradation via the PROTEASOME . (nih.gov)

10. Partial covalent labeling with pyridoxal 5'-phosphate induces bis(sulfosuccinimidyl)suberate crosslinking of band 3 protein tetramers in intact human red blood cells. (nih.gov)
SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. (neb.com)
However, most of our work is to put the CPPs and your target together by covalent links. (lifetein.com)

The H-NS protein is a global regulator of gene expression in bacteria and can also bind transposition complexes (transpososomes). (biomedcentral.com)
The protein encoded by this gene is similar to bovine and porcine proteins which accelerate transfer of certain glycosphingolipids and glyceroglycolipids between membranes. (nih.gov)

The modification of soy protein isolate (SPI) with different amounts of a naturally occurring cross-linking agent (genipin, Gen) and glycerol used as plasticizer was carried out in this work. (uchile.cl)

Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. (springeropen.com)

1. N-hydroxysulfosuccinimide active esters: bis(N-hydroxysulfosuccinimide) esters of two dicarboxylic acids are hydrophilic, membrane-impermeant, protein cross-linkers. (nih.gov)
Dimers of human erythrocyte band 3 subunits cross-linked at the extracytoplasmic membrane face. (nih.gov)
13. Detection of bis(sulfosuccinimidyl) suberate binding in electrophoresis: determination of membrane sidedness of proteins. (nih.gov)
17. Modulated red blood cell survival by membrane protein clustering. (nih.gov)
18. Organizational differences in the membrane proteins of normal and irreversibly sickled erythrocytes. (nih.gov)
Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. (gbiosciences.com)
Glycolipid transfer protein knockout disrupts vesicle trafficking to the plasma membrane. (nih.gov)

To determine whether a protein binds to filaments or monomers of actin. (cytoskeleton.com)
The actin binding protein spin-down assay kit provides G- or F-actin plus positive ( α -actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. (cytoskeleton.com)
F-actin binding proteins will co-sediment with actin filaments and form a pellet at the bottom of the centrifugation tube. (cytoskeleton.com)
Proteins with F-actin bundling activity can be detected since they will cause the F-actin to sediment at lower centrifugal forces than normal (14,000 x g vs 150,000 x g). (cytoskeleton.com)
F-actin severing proteins, G-actin binding proteins or non-actin binding proteins will stay in the supernatant. (cytoskeleton.com)
Severing proteins will be expected if more G-actin remains in the supernatant than in the negative control sample, and this activity should be tested further by measuring F-actin length distributions before and after adding the test protein. (cytoskeleton.com)
G-actin binding proteins can be measured by adding the test protein to G-actin and inducing polymerization, if the test protein sequesters G-actin then during the spin-down assay more actin will be left in the supernatant compared with the control. (cytoskeleton.com)
Both forms of actin interact with a plethora of proteins in the cell. (cytoskeleton.com)
To date there are over 50 distinct classes of Actin-Binding Proteins (ABPs), and the inventory is still far from complete. (cytoskeleton.com)
Actin Binding Proteins allow the actin cytoskeleton to respond rapidly to cellular and extracellular signals and are integral to cytoskeletal involvement in many cellular processes. (cytoskeleton.com)
The skeletal muscle actin spin-down kit may be more appropriate to use to study actin binding proteins from muscle tissues. (cytoskeleton.com)

Dr. Kuhlman and his colleagues, however, believe that by removing the limitations of a predetermined blueprint and taking cues from evolution they can more easily create functional proteins. (pharmaceuticalintelligence.com)

Hybridomas were screened in a solid-phase indirect enzyme-linked immunosorbent assay (ELISA) against a 2,4 TDI-human serum albumin (2,4 TDI-HSA) protein conjugate. (cdc.gov)
The properties of these MAbs (isotype and reactivity to various protein-isocyanate conjugate epitopes) were characterized using ELISA, dot blot, and Western blot analyses. (cdc.gov)
Western blot analyses demonstrated that some TDI conjugates form inter-and intra-molecular links, resulting in multimers and a change in the electrophoretic mobility of the conjugate. (cdc.gov)
The final conjugate will have a large linker. (lifetein.com)

Crosslinking can also be carried out in a two step system with reagents such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). (springeropen.com)
Sulfo-NHS is used to generate amine reactive esters from carboxylate groups and is primarily used to increase the efficiency of the carbodiimide EDC cross‐linking. (gbiosciences.com)

The SNAP-tag is a small protein based on human O 6 -alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein. (neb.com)

The densely packed and highly reactive surfaces allowed them to easily immobilize amino acids and proteins. (dojindo.com)
Chemical cross-linkers are molecules with a spacer region flanked by reactive end groups that are extremely specific, while others are not. (protrein.eu)
The chemical properties of the reactive groups and the length of the spacer region establish the limits of a cross-linker and therefore can be designed to accommodate different CLMS workflows. (protrein.eu)

References sequences are provided for genomes, transcripts, and proteins. (nih.gov)

Dual transcript and protein quantification in a massive single cell array. (stanford.edu)
The model RefSeq records produced from this pipeline have a distinguishing accession prefix (XM, XR, XP), are derived from the genomic sequence, have varying levels of transcript or protein homology support, and are not subject to further manual curation. (nih.gov)

In Sanger reaction DNFB is used to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary a. (gbiosciences.com)
Brzhozovskiy A, Kononikhin A, Bugrova AE, Kovalev GI, Schmit PO, Kruppa G, Nikolaev EN, Borchers CH . The Parallel Reaction Monitoring-Parallel Accumulation-Serial Fragmentation (prm-PASEF) Approach for Multiplexed Absolute Quantitation of Proteins in Human Plasma. (mcgill.ca)
Using a semi-specific cross-linker makes the data highly complex due to the large number of crosslinks formed in the reaction. (protrein.eu)

6. Cross-linking of wheat germ RNA polymerase II with dithiobis(succinimidyl propionate). (nih.gov)

For food protein hydrolysates, the substrate and enzymes are mixed in huge, tempered tanks, and require continuous stirring, as well as a constant temperature and pH level for several hours. (springeropen.com)

It is often unclear as to whether host proteins directly or indirectly regulate transposition reactions. (biomedcentral.com)

One of the major goals of Protein Structure Initiative (PSI) is to develop, apply and disseminate methods for faster, cheaper and more reliable determination of protein structure. (nih.gov)

Quantitative analysis of cross-links can reveal protein conformational and interaction changes that occur as a result of altered biological states, environmental conditions, or pharmacological perturbations. (tuberculosistest.net)
Our laboratory recently developed an isobaric quantitative protein interaction reporter (iqPIR) cross-linking strategy for comparative interactome studies. (tuberculosistest.net)
Multiplexed iqPIR is demonstrated on MCF-7 breast cancer cells treated with five different Hsp90 inhibitors revealing large scale protein conformational and interaction changes specific to the molecular class of the inhibitors. (tuberculosistest.net)
We propose that NCp7 binds to the RNA via a direct interaction of one zinc-binding motif to stem-loop 1 followed by binding of the other zinc-binding motif to stem-loop 1, stem-loop 2, or the linker region of the second RNA molecule, forming a bridge between the two RNAs. (nih.gov)

4 5 6 In most experimental systems, HIF-1α protein is constitutively expressed but gets rapidly degraded by the ubiquitin-proteasome pathway under normoxic conditions. (kosinmedj.org)
The relative abundance of distinct cross-links generated in each sample can be determined by adding isotopically labeled cross-linkers to samples from different experimental conditions. (protrein.eu)

Taken together these three networks are comprised of proteins which represent the major architectural building blocks of both the cytoplasm (classical cytoskeleton) and the nucleus (nucleoskeleton) of vertebrate cells. (nih.gov)

This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)

This can be done by protein engineering, chemical modification, immobilization or adding additives for stabilization. (springeropen.com)

Emerging roles for human glycolipid transfer protein superfamily members in the regulation of autophagy, inflammation, and cell death. (nih.gov)

Modeling of protein complex topology is one of the most common uses of CLMS to investigate how proteins are ordered with respect to one another as they form complexes, a process known as protein complex topology. (protrein.eu)

[ 23 ] The size and particulate nature of viruses is commonly thought to be responsible for their ability to directly cross the wall of lymphatic vessels into the lymphatic system. (medscape.com)

They have developed a computational protocol called SEWING that builds new proteins from connected or disconnected pieces of existing structures. (pharmaceuticalintelligence.com)
SEWING is a computational protocol that describes how to stitch together new proteins from connected or disconnected pieces of existing structures. (pharmaceuticalintelligence.com)
We can now begin to think about engineering proteins to do things that nothing else is capable of doing," said UNC's Brian Kuhlman, Ph.D. "The structure of a protein determines its function, so if we are going to learn how to design new functions, we have to learn how to design new structures. (pharmaceuticalintelligence.com)
Dr. Kuhlman's UNC team developed a protein design approach that emulates natural mechanisms for shuffling tertiary structures such as pleats, coils, and furrows. (pharmaceuticalintelligence.com)
Putting the approach into action, the UNC team mapped 50,000 stitched together proteins on the computer, and then it produced 21 promising structures in the laboratory. (pharmaceuticalintelligence.com)
The study of protein structures and interactions is critical to understand their function. (tuberculosistest.net)

Several ESRs are working on developing new tools and data analysis pipelines to improve cross linking mass spectrometer data analysis. (protrein.eu)

Tertiary protein structure modeling can benefit from High Density (HD) CLMS. (protrein.eu)
Using this information can help exclude certain arrangements of the secondary structure elements of the proteins, thereby supporting modeling of the tertiary structure. (protrein.eu)

9. Cross-linking and chymotryptic digestion of the extracytoplasmic domain of the anion exchange channel in intact human erythrocytes. (nih.gov)
A Robust Protocol for Protein Extraction and Digestion. (stanford.edu)

it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. (gbiosciences.com)
For improved assay sensitivity, minimal non-specific binding, and a high signal-to-background ratio, NAP-BLOCKER™, an animal-free protein blocking agent, is recommended. (gbiosciences.com)
Read about the NEB's set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins. (neb.com)

16. Differential induction of macrophage recognition of carrier erythrocytes by treatment with band 3 cross-linkers. (nih.gov)

Many scientists have concerns about the bulky size of the linker. (lifetein.com)

Traditionally, researchers have used computational protein design to recreate in the laboratory what already exists in the natural world. (pharmaceuticalintelligence.com)
Currently, the UNC researchers are using SEWING to create proteins that can bind to several other proteins at a time. (pharmaceuticalintelligence.com)
CLMS allows researchers to investigate proteins in solution, capturing them in a dynamic state that is closer to physiological settings. (protrein.eu)

We were excited that some had clefts or grooves on the surface, regions that naturally occurring proteins use for binding other proteins," said the Science article's first author, Tim M. Jacobs, Ph.D., a former graduate student in Dr. Kuhlman's laboratory. (pharmaceuticalintelligence.com)

However, some drugs contain bulky linkers without issues or side effects. (lifetein.com)

Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. (deshaieslab.com)
When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal and complementary labeling of two proteins simultaneously in the same cells. (neb.com)

Anatomy19

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Phenomena and Processes81

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Cleavable5

Peptides7

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Structural5

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Vitro4

19822

Residues3

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Crystallography2

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Purification1

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Gene2

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