These mitotic kinesins are spatially and temporally regulated to stringently control their localized activity towards both ends of MTs at each stage of mitosis, thereby ensuring mitotic fidelity [ 3 ]. (biomedcentral.com)
The pre-replication complex (pre-RC) assembly or the DNA replication licensing is the first step in DNA replication initiation, characterized by the sequential recruitment of ORCs, Cdc6, Cdt1 and MCMs to the DNA replication origins to form the pre-RC at the end of mitosis ( Bell and Dutta 2002 ). (intechopen.com)
Our data reveal a spindle-directed signalling pathway that regulates SCAR activity, migration and adhesion at mitosis. (biologists.com)
Highly regulated2
Cell cycle regulation of centrosome duplication Centrosomes are only supposed to replicate once in each cell cycle and are therefore highly regulated. (wikipedia.org)
The spindle microtubule (MT) exhibits highly regulated dynamic instability, with frequent polymerization and depolymerization occurring at both the plus and minus ends. (biomedcentral.com)
Centrosomes6
Centrosomes are the major microtubule organizing centers (MTOC) in mammalian cells. (wikipedia.org)
When centrosomes fail to recruit γ-tubulin complexes, they still nucleate microtubules via the TOG domain protein Mini-spindles (Msps), but these microtubules have different dynamic properties. (bvsalud.org)
Our data, therefore, help explain the dispensability of the GCP4/5/4/6 core and highlight the robustness of centrosomes as microtubule organizing centers. (bvsalud.org)
Plant cells lack centrosomes and instead utilize acentrosomal microtubule organizing centers (MTOCs) to rapidly increase the number of microtubules at the onset of spindle assembly. (bvsalud.org)
Centrosomes are the major microtubule organizing structures in vertebrate cells. (biomedcentral.com)
In animal cells, centrosomes represent the major microtubule-organizing structures (MTOC). (biomedcentral.com)
Spindle6
Since the centrosome organizes the microtubules of a cell, it has to do with the formation of the mitotic spindle, polarity and, therefore, cell shape, as well as all other processes having to do with the mitotic spindle. (wikipedia.org)
At spindle poles, where microtubule minus ends are concentrated, microtubule nucleation and depolymerization, the latter required for poleward microtubule flux, happen side by side. (bvsalud.org)
We find that the spindle pole-localized kinesin-13 KIF2A is a microtubule minus-end depolymerase, in contrast to its paralog MCAK. (bvsalud.org)
Together, these results provide insight into the molecular mechanisms by which a minimal protein module coordinates microtubule nucleation and depolymerization at spindle poles consistent with their role in poleward microtubule flux. (bvsalud.org)
Depletion of PIP4KIIγ enhanced the spindle pole accumulation of mitotic centromere-associated kinesin (MCAK), a microtubule (MT)-depolymerizing kinesin, and resulted in a less stable spindle pole-associated MT. Depletion of MCAK can ameliorate PIP4KIIγ depletion-induced spindle abnormalities. (biomedcentral.com)
In addition, coordination of MT polymerization/depolymerization at both ends to maintain the size and shape of mitotic spindles is also critical for the proper formation and function of the mitotic spindle. (biomedcentral.com)
Depolymerization2
How these seemingly antagonistic processes of nucleation and depolymerization are coordinated is not understood. (bvsalud.org)
These results indicate that PIP4KIIγ and PIP2 may negatively regulate the MT depolymerization activity of MCAK by reducing PLK1-mediated phosphorylation of MCAK. (biomedcentral.com)
Vertebrate cells1
These data are the first to characterize the dynamics of centrosome-associated γ-tubulin in vertebrate cells in vivo and to demonstrate the microtubule-independent nature of these dynamics. (silverchair.com)
Nucleate1
For example, the mother centriole can nucleate and organize microtubules, whereas the daughter centriole can only nucleate. (wikipedia.org)
Tightly regulated2
Centrosome duplication is tightly regulated and limited at once per cell cycle through a mechanism that prevents re-duplication. (biomedcentral.com)
This continues for 10 minutes after division before the daughter cells revert to normal random motility, indicating that this is a tightly regulated process. (biologists.com)
Actin1
We show that the actin nucleation factor SCAR/WAVE is strongly activated during Dictyostelium cytokinesis. (biologists.com)
Efficient γTuRC uncapping requires the combined action of KIF2A and a microtubule severing enzyme, leading to treadmilling of the uncapped microtubule driven by KIF2A. (bvsalud.org)
Signaling pathway1
In addition, CRB3-depleted cells are unresponsive to the activation of the Hh signaling pathway, while CRB3 regulates the Wnt signaling pathway. (bvsalud.org)
Centriole1
To template these cilia, each MCC undergoes a process termed centriole amplification to produce hundreds of centrioles, barrel-shaped microtubule structures that form the base upon which cilia are assembled. (elifesciences.org)
Protein4
CP110 is an important centrosomal protein that is phosphorylated by both mitotic and interphase Cdk/cyclin complexes and is thought to influence centrosome duplication in the S phase. (wikipedia.org)
To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
The PCM is an ordered lattice that anchors a large number of microtubule (MT)-associated proteins, many of which bear putative coiled-coil domains, a tertiary structure known to facilitate protein-protein interactions [ 4 ]. (biomedcentral.com)
We also find that the microtubule end-binding protein EB1 is required to restrict SCAR localisation and direct migration. (biologists.com)
The primary cilium plays important roles in regulating cell differentiation, signal transduction, and tissue organization. (bvsalud.org)
The overarching goal of the research in my lab is to understand how signal transduction pathways regulate morphogenesis - the emergence of spatial organization - during development. (stanford.edu)
It has also been shown that Cdk2 complexes with both cyclin A and cyclin E and this complex is critical for centrosome duplication. (wikipedia.org)
Processes1
In multicellular organisms, signal transduction is essential to these processes, yet while our understanding of how signals regulate gene expression is relatively advanced, our understanding of how signals direct the acquisition of specific shapes and forms is less advanced. (stanford.edu)
Mitotic1
In this study, we explored how PIP4KIIγ regulates the assembly of mitotic spindles. (biomedcentral.com)
Reveal1
Therefore, our studies reveal the molecular mechanisms by which CRB3 recognizes Rab11-positive endosomes to facilitate ciliogenesis and regulates cilium-related signaling pathways in tumorigenesis. (bvsalud.org)
Generate1
Each MCC contains hundreds of motile cilia, microtubule-based organelles that generate the motive force to move fluid over the surface of the cell. (elifesciences.org)
Cell cycle2
In this chapter, we mainly discuss the coordination regulations between DNA replication initiation and other cell cycle events that ensure genomic integrity. (intechopen.com)
DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
Activity1
Due to its asymmetric activity, KIF2A still allows microtubule nucleation from the γ-tubulin ring complex (γTuRC), which serves as a protective cap shielding the minus end against KIF2A binding. (bvsalud.org)
Major1
However, a major unresolved question is how each cell regulates the precise number of its motile cilia during differentiation. (elifesciences.org)
Dynamic1
They also suggest that the dynamic properties of microtubules are influenced by how they are nucleated. (bvsalud.org)
Cells1
In each case, PCP is critical to the function of these cells and tissues, and errors in the signaling system controlling PCP lead to human diseases and developmental defects, including congenital deafness, neural tube closure defects and cardiac outflow tract anomalies. (stanford.edu)
Complex1
Here, we show that CRB3, as a navigator, regulates vesicle trafficking in γ-tubulin ring complex (γTuRC) assembly during ciliogenesis and cilium-related Hh and Wnt signaling pathways in tumorigenesis. (bvsalud.org)
Assembly1
Thus, the proper assembly of hundreds of motile cilia is critical for the functions of MCC, and the overall health of the associated tissues. (elifesciences.org)