Gene Knockdown TechniquesClustered Regularly Interspaced Short Palindromic RepeatsInverted Repeat SequencesRNA InterferenceRNA, Small InterferingCRISPR-Associated ProteinsCRISPR-Cas SystemsZebrafishLentivirusZebrafish ProteinsMorpholinosGene SilencingDNA, IntergenicStreptococcus thermophilusCell Line, TumorEmbryo, NonmammalianRNA, GuideRNA, ArchaealGenetic VectorsOligonucleotides, AntisenseAnimals, Genetically ModifiedBase SequenceGenetic TechniquesGene TargetingGreen Fluorescent ProteinsDoxycyclineCell LineGene Expression Regulation, DevelopmentalCell ProliferationSignal TransductionRNA CleavageTransfectionMolecular Sequence DataGene Expression RegulationRNA, MessengerCells, CulturedReverse Transcriptase Polymerase Chain ReactionSulfolobus solfataricusRNA, Double-StrandedApoptosisArchaeal VirusesDown-RegulationCell MovementHeLa CellsBlotting, WesternPectobacteriumGenome, ArchaealPromoter Regions, GeneticStreptococcus PhagesBacteriophagesTransgenesCell DifferentiationLarvaMutationCell SurvivalGene ExpressionPhenotypeDNA CleavageTranscription FactorsRepetitive Sequences, Nucleic AcidPhosphorylationModels, BiologicalGene Expression Regulation, ArchaealRNA, BacterialMice, KnockoutGenome, BacterialProtein-Serine-Threonine KinasesMice, Inbred C57BLProphagesArchaeal ProteinsFlow CytometryPseudomonas PhagesMice, TransgenicPyrococcus furiosusDisease Models, AnimalNeuronsArchaeaGenetic LociThermoproteusRNA Processing, Post-TranscriptionalDNA, BacterialHEK293 CellsNucleotide MotifsSequence Analysis, DNAPlasmidsTime FactorsThermococcalesClostridium thermocellumHaloferax mediterraneiPyrococcus abyssiDNA, ArchaealProtein BindingGenetic EngineeringErwinia amylovora