PSII core complex is formed by two reaction center proteins D1 (PsbA) and D2 (PsbD), along with intrinsic antenna CP43 (PsbC) and CP47 (PsbB). (elifesciences.org)
Psb34 protein modulates binding of high-light-inducible proteins to CP47-containing photosystem II assembly intermediates in the cyanobacterium Synechocystis sp. (alga.cz)
Photosystem II antenna modules CP43 and CP47 do not form a stable 'no reaction centre complex' in the cyanobacterium Synechocystis sp. (alga.cz)
PSII10
Additionally, the PSBS protein involved in NPQ and the minor PSII antenna protein LHCB4 were probed. (agrisera.com)
Mutation of residues that would disrupt the donor side primary electron transfer pathway in PSII do not inhibit the production of Chl f, while alteration of the putative ChlZ, P680 and QA binding sites rendered ChlF non-functional. (bvsalud.org)
To maintain photosynthetic activity, the PSII reaction center protein D1, which is the primary target of unavoidable photo-oxidative damage, is efficiently degraded by FtsH protease. (elifesciences.org)
Further characterization of Trp-14 using chloroplast transformation in Chlamydomonas indicated that substitution of D1 Trp-14 to Phe, mimicking Trp oxidation enhanced FtsH-mediated D1 degradation under high light, although the substitution did not affect protein stability and PSII activity. (elifesciences.org)
In photosynthetic organisms, maintenance of photosynthetic light reaction is manifested by so called Photosystem II (PSII) repair system, where the reaction center protein D1 is targeted to photo-oxidative damage and rapidly degraded by the processive protease FtsH. (elifesciences.org)
The work supports a role for chemical modification in the recognition and subsequent degradation of a key protein subunit of PSII by a bacterial-type protease, suggesting that tryptophan oxidation of components of the photosynthetic apparatus after high light stress plays a critical role in initiating the PSII repair system. (elifesciences.org)
The first step of photosynthetic electron transfer in the thylakoid membrane occurs at Photosystem II (PSII), where light energy absorbed by P 680 chlorophyll molecules drives water oxidation, and electron is transferred to plastoquinone. (elifesciences.org)
FtsH4 protease controls biogenesis of the PSII complex by dual regulation of high light-inducible proteins. (alga.cz)
LHCSR3 ( A ) and LHCSR1 ( B ) content was then normalized to the amount of CP43 as a reference for PSII. (elifesciences.org)
For PSII, the accumulation of the essential subunits PsbB (CP43) and PsbD (D2) and the LHCB1 antenna protein were determined, while for the cytochrome b6f complex, the accumulation of the essential redox-active subunits PetA (cytochrome f), PetB (cytochrome b6), and PETC (Rieske FeS protein) was tested. (agrisera.com)
Subunit1
Accumulation of Cyanobacterial Photosystem II Containing the 'Rogue' D1 Subunit Is Controlled by FtsH Protease and Synthesis of the Standard D1 Protein. (alga.cz)
Subunits3
Altered protein accumulation and stability of the chloroplast ATP synthase in the cgl160 mutant visualized by immunoblotting.A. Immunoblots with antibodies against essential subunits of the photosynthetic protein complexes of wild-type (Col-4) Arabidopsis and the two cgl160 T-DNA insertion lines grown under long-day and short-day conditions. (agrisera.com)
Accumulation of the cytochrome b6f complex was probed with antibodies against the essential subunits PetA (cytochrome f), PetB (cytochrome b6), and PETC (Rieske protein). (agrisera.com)
Immunoblot analysis was performed using specific antibodies recognizing PsaA, CP43, LHCSR3 ( a ), and LHCII subunits. (elifesciences.org)
UniProt1
Universal protein resource accession number Q55274 for "Iron stress-induced chlorophyll-binding protein" at UniProt. (wikipedia.org)
Photosynthetic1
Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization. (alga.cz)
Mesophyll2
1 µg of chlorophyll from Pisum sativum (1) , Mesembryanthemum crystallinum (2) , mesophyll (3) and bundle sheath (4) of Zea mays , mesophyll (5) and bundle sheath (6) of Echinochloa crus-galli chloroplasts extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl2 and 2 mM EDTA were loaded to lanes. (agrisera.com)
1.0 µg of chlorophyll from mesophyll (M) and bundle sheath (BS) thylakoids of various C4 plants ( Echinochloa crus-galli, Panicum miliaceum, Zea mays ) extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl2 and 2 mM EDTA. (agrisera.com)
Photosystem2
IsiA is also synthesized in cells grown under high light irradiances, protecting from photodamage Photosystem II light-harvesting protein Berera R, van Stokkum IH, d'Haene S, Kennis JT, van Grondelle R, Dekker JP (March 2009). (wikipedia.org)
Assembly of D1/D2 complexes of photosystem II: Binding of pigments and a network of auxiliary proteins. (alga.cz)
Cyanobacteria4
Iron-starvation-induced protein A, also known as isiA, is a photosynthesis-related chlorophyll-containing protein found in cyanobacteria. (wikipedia.org)
Aggregates of the chlorophyll-binding protein IsiA (CP43') dissipate energy in cyanobacteria" (PDF). (wikipedia.org)
In cyanobacteria that undergo far red light photoacclimation (FaRLiP), chlorophyll (Chl) f is produced by the ChlF synthase enzyme, probably by photo-oxidation of Chl a. (bvsalud.org)
Mutations Suppressing the Lack of Prepilin Peptidase Provide Insights Into the Maturation of the Major Pilin Protein in Cyanobacteria. (alga.cz)
Accumulation1
Because the accumulation of most tested proteins was highest under high-light conditions, lanes one to three contain samples diluted to 25%, 50%, and a 100% sample of wild-type tobacco grown under high-light conditions, to allow for semi-quantitative determination of changes in protein abundance. (agrisera.com)
Photoprotection1
It belongs to the chlorophyll-a/b-binding family of proteins, and has been shown to have a photoprotection role in preventing oxidative damage via energy dissipation. (wikipedia.org)
Light1
Total protein extracts from low-light (LL) or high-light (HL) acclimated cells were loaded on a chlorophyll basis (μg of chlorophylls loaded are reported in the figure). (elifesciences.org)
High1
It was originally identified under Fe starvation, and thus received the name iron-starvation-induced protein A. However, the protein has more recently been found to respond to a variety of stress conditions such as high irradiance. (wikipedia.org)
Fluorescence4
In this study, photosynthetic characteristics of four different Triticeae crops (wheat, triticale, barley, and highland barley) were investigated based on chlorophyllfluorescence and the level of photosynthetic proteins under high light. (bvsalud.org)
Photosynthetic performance in TRV-VIGS-treated and uninfiltrated plants was assessed by application of CO 2 exchange, chlorophyllfluorescence, and in vivo absorbance changes at 810 nm. (scirp.org)
All double mutants were viable and indistinguishable from the wild type when grown under low light conditions, but - except for sex1-3/tpt-2 - developed a high chlorophyllfluorescence (HCF) phenotype and growth retardation when grown in high light. (biomedcentral.com)
Immunoblots of thylakoid proteins, Blue-Native gel electrophoresis and chlorophyllfluorescence emission analyses at 77 Kelvin with the adg1-1/tpt-2 double mutant revealed that HCF was linked to a specific decrease in plastome-encoded core proteins of both photosystems (with the exception of the PSII component cytochrome b 559 ), whereas nuclear-encoded antennae (LHCs) accumulated normally, but were predominantly not attached to their photosystems. (biomedcentral.com)
Thylakoid3
The molecule of α-Toc/α-TQ is located close to chlorophyll a 614 of one of the Lhcb1 proteins and its chromanol/quinone head is exposed to the thylakoid lumen. (ciisb.org)
1998). Despite this progress, however, it is still not known how the various proteins in the thylakoid membranes cooperate to convert the light energy so efficiently. (studylib.net)
This type of knowledge is very important, also because structural rearrangements in the thylakoid membranes form the basis of several regulatory processes of the photosynthetic light reactions (e.g., the state transitions, non-photochemical quenching and the xanthophyll cycle, the extremely rapid turnover of some PS II proteins and the repair mechanisms occurring after photoinhibitory damage). (studylib.net)
Complexes3
But in other cases, including the slow rate of plastoquinol oxidation, there has been no compensatory increase in the abundance of catalytically limiting protein complexes. (biomedcentral.com)
Cells of the symbiotic prochlorophyte Prochloron didemni were separated from its ascidian partner, thylakoids isolated, treated to liberate chlorophyll-containing complexes, and negatively stained preparations observed by transmission EM. (ucsd.edu)
Within the last decade, impressive progress has been made on the structure and organization of essential parts of the protein complexes involved in the photosynthetic light reactions (see, e.g. (studylib.net)
Analyses1
Gene expression and subcellular localization analyses reveal that FAME is a membrane-bound protein enriched in the kidneys. (ciisb.org)
Core2
a , The view of the C 2 S 2 supercomplex from the lumenal side with indicated subunits of light-harvesting antenna, Lhcb5, Lhcb8 and the S-LHCII trimer, bound to the dimeric core complex. (ciisb.org)
The image shows an averaged top view of a chlorophyll-photsystem II super complex with an overlay illustrating the putative locations of the core dimer and surrounding CP43 proteins obtained from x-ray analysis data. (ucsd.edu)
Gene2
To assess transient complementation of NPQ, the endogenous psbS gene was silenced using only the transit sequence in the TRV vector followed by Agrobacterium-mediated transient expression of a modified gene consisting of an altered transit sequence fused to the native mature protein sequence. (scirp.org)
The exponential growth in gene sequence information has led to use of reverse genetics to dissect complex functions by selective protein elimination. (scirp.org)
Plants1
PsbS protein was undetectable and both psbS mRNA transcript levels and NPQ capacity were dramatically reduced in new leaf tissue of VIGS-psbS plants only. (scirp.org)
Shown1
It belongs to the chlorophyll-a/b-binding family of proteins, and has been shown to have a photoprotection role in preventing oxidative damage via energy dissipation. (wikipedia.org)