Covalently bound to proteins. Medical search. Frequent questions

Here we report the development of small molecules that irreversibly bind to a common oncogenic mutant, K-Ras(G12C). (rcsb.org)
ABC (ATP binding cassette) transporters catalyze the transport of a wide variety of molecules across lipid bilayers into or out of cells and organelles. (rug.nl)
Small molecules capable of acylating this prosthetic group will provide a simple and reversible means of introducing novel functionality onto carrier protein domains. (rsc.org)
Targeting one of these sites with covalently tethered molecules or mutations allosterically inhibits enzyme activity. (rcsb.org)
Over the last decades, a variety of RNA methods have been developed for the study of RNA-DNA, RNA-RNA, and RNA-protein interactions including RNA complexes with ligand molecules. (biosyn.com)
RNA molecules are functionally diverse and involved in many cellular processes such as catalysis, ligand binding, and protein recognition. (biosyn.com)
RNA molecules in solution may adopt secondary structures that are only partially determined by thermodynamics since RNA molecules can undergo conformational changes during interaction with other RNAs, RNA binding proteins or RNA binding peptides. (biosyn.com)
Knowledge of the three-dimensional structures of the carbo-hydrate molecules is indispensable for a full understanding of the molecular processes in which carbohydrates are involved, such as protein glycosylation or protein-carbohydrate interactions. (iucr.org)
Further, these molecules were covalently docked with isocitrate lyase using SeeSAR. (biosolveit.de)
The affinity of selected molecules towards the defined binding site was noted and that lead to the discovery of forty three molecules which have lesser inhibition constant when compared to Itaconate. (biosolveit.de)
Then these molecules were covalently docked using SeeSAR. (biosolveit.de)
Then its binding site was defined for estimating the affinity of screened molecules against the protein. (biosolveit.de)
Different poses of the chosen molecules were generated and covalently docked. (biosolveit.de)
These are subsequently covalently linked to yield hybrid molecules, whose affinities and specificities for a given target are studied and continuously improved. (uni-due.de)
Will the presence of molecules that are covalently added on the protein through using the No-Stain Protein Labeling Reagent affect the ability of the labeled protein to bind antibody and thus impact the resultant immunodetection signal? (thermofisher.com)
Scientists at the Pacific Northwest National Laboratory in Richland, Wash., have used an established technique to observe real-time interactions between single protein molecules for the first time. (the-scientist.com)
PGs, molecules which consist of a protein core that is covalently modified with GAG chains, are distributed both to the ECM "proper" associated with the cell membrane as well as located to intracellular compartment. (hindawi.com)
However, most drugs act as haptens, binding covalently to serum or cell-bound proteins, including peptides embedded in major histocompatibility complex (MHC) molecules. (msdmanuals.com)
Haptens may also bind directly to class II MHC molecules, directly activating T cells. (msdmanuals.com)
Recent oxygen-17 magnetic relaxation dispersion (MRD) experiments have shown that the vast majority of water molecules in the protein hydration layer suffer a mere twofold dynamic retardation compared with bulk water. (lu.se)

One of the ABC transporters that is studied in detail is the osmoregulatory ABC transporter OpuA (class D). Osmotic control of OpuA involves gating by intracellular ionic strength and is mediated by lipid-protein interactions. (rug.nl)
The mapping of RNA-protein or RNA-RNA interactions by protein pull-down or affinity pull-down methods allow studying RNA structures, as well as RNA-protein, and RNA-RNA interactions. (biosyn.com)
In particular, the identification of dominant interactions of electrostatics and hydrophobicity was observed given the sensitivity of binding assays to salt disruption and the manipulation of block polymer copolymer architecture. (umass.edu)
Furthermore, limitations of current assays to quantify polymer-protein binding were discussed and a new method of Covalent- Labeling Mass Spectrometry was introduced to quantify non-covalent polymer interactions with model protein surface patches. (umass.edu)
This represents the first use of this method for identifying non-covalent polymer-protein interactions. (umass.edu)
This method allowed for increased understanding of the binding interactions between polymer-based CPPMs and anionic and hydrophobic surface patches on protein cargo. (umass.edu)
The role of polymer self-assembly was observed to impact not only initial binding interactions with protein cargo, but also internalization and intracellular activity. (umass.edu)
Proposed by Peter Wolynes at the University of California, San Diego, 2 this theory states that weak interactions between relatively unstructured proteins initiate protein-protein binding. (the-scientist.com)
To make proteins, such as binding, recognition and catalysis, often progress, it is therefore helpful to adopt a sceptical atti- involve specific interactions with individual water mol- tude. (lu.se)

Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. (biosyn.com)
With solvent-assisted digestion (80% acetonitrile in digestion solution), the protein was digested for peptide identification, especially for some peptides with low mass. (cdc.gov)
Meanwhile, 4 peptides covalently bound to phenols were identified. (bvsalud.org)

Approximately 30% of melphalan is (covalently) irreversibly bound to plasma proteins. (nih.gov)
The arsenic covalently binds to the sulfhydral group in cysteine, thereby becoming irreversibly bound to the hair. (cdc.gov)

A small high affinity ligand, biotin, was covalently attached to loop 6 of OmpG and used to capture biotin-binding proteins. (umass.edu)
Signaling is initiated when the Wnt ligand binds to the Frizzled receptor on the cell membrane and the LDL receptor-associated protein 5/6 (LRP5/6) co-receptor. (frontiersin.org)
One mechanism underlying CRPC and resistance to hormonal therapies is the expression of constitutively active splice variant(s) of androgen receptor (AR-Vs) that lack its C-terminus ligand-binding domain. (bcgsc.ca)
Please, note that similar approaches have been suggested earliear, but primarily for ligand-binding studies, whereas our approach is directed towards studies of enzyme active sites, for which the QM system is covalently bound to the MM system. (lu.se)

The chemical reactions and pathways resulting in the formation of cell wall mannoproteins, any cell wall protein that contains covalently bound mannose residues. (yeastgenome.org)
The No-Stain Protein Labeling Reagent provides two active ingredients that result in a fluorophore being covalently attached to the side chains of some of the lysine residues of a protein. (thermofisher.com)
includes residues 201 to 321 of WASP, which contains the CRIB domain essential for Cdc42 binding. (the-scientist.com)
Thin-layer chromatography of dinitrophenyl derivatives prepared from Pronase-digested microsomal protein and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis of solubilized microsomes indicated that the radiolabeled products were covalently bound to amino acid residues of microsomal protein. (aspetjournals.org)

Enzymes (and other proteins) are composed of amino acid chains called polypeptide chains. (newworldencyclopedia.org)
Interacting selectively and non-covalently with a domain within the same polypeptide. (yeastrc.org)
Some protein and large polypeptide drugs (eg, insulin , therapeutic antibodies) can directly stimulate antibody production. (msdmanuals.com)
Flavinylation of Fp in B. subtilis does not require the Ip subunit or the membrane anchor but depends on folding of the Fp polypeptide into a state that can bind FAD and subsequent mediate covalent binding of the FAD [3, 4]. (lu.se)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)
The B. subtilis Fp polypeptide was found to lack covalently bound FAD when produced in E. coli although E. coli Fp in the same cell was flavinylated [8]. (lu.se)

Serum albumin is the major binding protein, accounting for approximately 40% to 60% of the plasma protein binding, while α 1 -acid glycoprotein accounts for about 20% of the plasma protein binding. (nih.gov)
Here, we study the interaction of PEG-covered carbon nanotubes (CNTs) with the serum complement protein C1q. (nih.gov)
Our work was the first revelation that a "noisy" nanopore sensor could not only discriminate among homologous proteins but could maintain this sensitivity and specificity in a serum sample. (umass.edu)
The effects of gender and alcohol consumption on serum enzymes, protein and bilirubin in heavy, moderate and non-drinkers were investigated. (scialert.net)
Whole blood was taken via puncture of the cubical vein and serum protein, albumin, total and direct bilirubin concentrations were determined using Synchron CX 5 autoanalyzer. (scialert.net)
Serum protein, albumin and bilirubin levels were significantly different in both male and female heavy and moderate drinkers. (scialert.net)
Serum protein, albumin and bilirubin levels were impaired by alcohol consumption in both males and females and may provide additional information in the diagnosis and management of alcoholism. (scialert.net)

Non-covalently bound complexes, which can include membrane and soluble proteins as well as nucleotides and ligands, play an essential role in many cellular processes. (europa.eu)
This allows us to assess binding strength of biomolecular complexes. (europa.eu)
The Protein Data Bank (PDB) is a valuable resource for three-dimensional structural information on glycoproteins and protein-carbohydrate complexes. (iucr.org)
New assays to investigate competition of polymer-cargo complexes by intracellular proteins were introduced and revealed that competition of xii polymer-cargo is influenced by initial binding strength. (umass.edu)
These results indicated that protein-phenol covalent complexes had great potential as functional components in the food field. (bvsalud.org)

These compounds rely on the mutant cysteine for binding and therefore do not affect the wild-type protein. (rcsb.org)
Organophosphorus (OP) compounds can bind covalently to many types of proteins and form protein adducts. (cdc.gov)
Binds to protein and other compounds containing SH group. (medscape.com)
Pin1 inhibition improves the efficacy of ralaniten compounds that bind to the N-terminal domain of androgen receptor. (bcgsc.ca)

van den Bogaart, G., Krasnikov, V., and Poolman, B. (2006) Dual-color fluorescence burst analysis to probe protein diffusion through the mechanosensitive channel MscL. (rug.nl)
We investigate the mobility of proteins in the bacterial cytoplasm and cytoplasmic membrane as well as membrane model systems, using optical microscopy-based techniques such as fluorescence correlation spectroscopy (FCS) and pulsed-FRAP (panel a). (rug.nl)
The fluorescence properties of a labeled protein and free dye are very similar. (forextrading-madeeasy.com)
Characterization and quantification of polymer-protein binding relationships were established using fluorescence quenching assays. (umass.edu)
The No-Stain Protein Labeling Reagent is compatible with a wide-range of imagers that have a UV, Green LED, or fluorescence light source, for example, the iBright imagers. (thermofisher.com)
Whereas most fluorescent labels require genetic modification of target proteins, I-SO attaches covalently to the protein of interest and changes its fluorescence during a binding event. (the-scientist.com)
Fourier-transform infrared, circular dichroism and fluorescence emission spectroscopy showed that the binding of phenol can change the secondary/tertiary structure of protein. (bvsalud.org)

How does crowding affect mobility of proteins and lipids? (rug.nl)
This includes for example the screening of potential drugs against Alzheimer's disease, or the development of handling procedures that allow the investigation of membrane proteins in dependence of the lipids which compose the cell membranes. (europa.eu)
From this procedure tailored ligands evolve for proteins, lipids and nucleic acids, which are able to interfere with biological processes by entirely new mechanisms of action. (uni-due.de)

Hair contains a high content of the protein, keratin, which is rich in the amino acid, cysteine. (cdc.gov)
Raney nickel desulfunization of Pronase hydrolysates released several HCP metabolites that were apparently bound to sulfur-containing amino acids such as cysteine and homocysteine. (aspetjournals.org)
The most common resistance mechanism in patients whose disease progresses on covalent BTK inhibitors (BTKis) is a mutation in the BTK 481 cysteine residue to which the inhibitors bind covalently. (lu.se)

Most enzymes are proteins , though certain nucleic acids , called ribozymes , are also capable of catalytic activity. (newworldencyclopedia.org)

Biotin is covalently linked to the protein surface. (tcichemicals.com)
According to the lectin-blotting analysis, the rMOA-Biotin specifically binds to α(1-3)Gal-terminated Cetuximab but not to non-α(1-3)galactosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B. (tcichemicals.com)

If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

OmpG specifically interacted with target proteins and with a high sensitivity. (umass.edu)
In addition, we found that OmpG could discriminate among eight target proteins, four avidin homologues and four antibody homologues. (umass.edu)

Covalent binding of the organophosphate insecticide profenofos to tyrosine on α- and β-tubulin proteins. (cdc.gov)
The thus created gas phase ions are comparably low charged and depending on the intensity of the desorption laser the non-covalent bonds between the proteins can be preserved or destroyed. (europa.eu)
To investigate the effects of structure, multiple binding sites and antioxidant property of Tartary buckwheat protein-phenols covalent complex, protein was combined with different concentrations of phenolic extract. (bvsalud.org)
The covalent bond to FAD in SQR is essential for enzyme activity and the main reason for this is that the bond raises the midpoint redox potential of the flavin by more than 80 mV compared to non-covalently bound flavin. (lu.se)

eg, penicillin itself is not antigenic, but its main degradation product, benzylpenicilloic acid, can combine with tissue proteins to form benzylpenicilloyl (BPO), a major antigenic determinant. (msdmanuals.com)

SQR consists of a membrane-peripheral heterodimer domain (often called succinate dehydrogenase) which is tightly bound to a membrane-spanning anchor, which is a cytochrome with one or two heme groups depending on the organism. (lu.se)

Binding of these inhibitors to K-Ras(G12C) disrupts both switch-I and switch-II, subverting the native nucleotide preference to favour GDP over GTP and impairing binding to Raf. (rcsb.org)
The enhancement in binding of [ 14 C]HCP to microsomal protein in the presence of epoxide hydrase inhibitors was consistent with the formation of HCP arene oxides or structurally related species. (aspetjournals.org)

Previous work in the Tew group has demonstrated polymer cell-penetrating peptide mimics (CPPMs) as effective transporters of biological agents, including proteins and antibodies. (umass.edu)
As antibodies and protein antigens are more stable than RNA, how patient samples are transported and handled isn't as critical for accurate detection, which reduces the number of potential false-negatives 3,4 . (abcam.com)

Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). (ibecbarcelona.eu)
With the full complement of prosthetic groups they are then bound to the heme-containing membrane-anchor to form the active SQR. (lu.se)

HA is synthesized in the absence of a protein core at the inner face of the plasma membrane and consequently found in the form of free chains whereas other GAG types are covalently bound into protein cores to form proteoglycans (PGs). (hindawi.com)

Post-translational modifications (PTMs) of proteins, including phosphorylation, acetylation, ubiquitination, and SUMOylation, can regulate the function of proteins, determine the active state and subcellular location of proteins, and dynamically interact with other proteins related to carcinogenesis and progression ( 17 - 20 ). (frontiersin.org)

This lectin preferably binds to a [Galα(1-3)Gal] glycan epitope. (tcichemicals.com)

The binding makes the protein-drug complex immunogenic, stimulating antidrug antibody production, T-cell responses against the drug, or both. (msdmanuals.com)

It is found that the cryptochrome binding to the ISCA1 polymer is not uniform and that the binding affinity depends on its placement along the ISCA1 polymer. (nature.com)

When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. (bvsalud.org)

Since RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression precise knowledge of their binding sites is critical for determining their molecular function and for understanding their roles in cell development and disease. (biosyn.com)

Oncogenic mutations result in functional activation of Ras family proteins by impairing GTP hydrolysis. (rcsb.org)

The MALDI-TOF-MS and LC-ESI-TOF-MS analysis results showed that profenofos bound covalently to Tyr83 in porcine a-tubulin (TGTY*83R) and to Tyr281 in porcine ß-tubulin (GSQQY*281R) with a mass increase of 166.02 Da from the original peptide fragments of porcine tubulin proteins. (cdc.gov)
Finally, future directions for designing next generation cell-penetrating peptide mimics as carriers of protein cargo were discussed. (umass.edu)
This understanding can be applied beyond cell penetrating peptide mimics to the design of smart carriers that are capable of binding to a range of novel cargo, have controllable cargo release properties, and even preferentially binding to proteins. (umass.edu)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)

The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)
The 414K-MDI conjugation appears to covalently cross- link GSH to albumin via GSH's NH2-terminus, a unique conformation possibly resulting from cyclized mono(GSH)-MDI or asymmetric ( S , N -linked) bis(GSH)-MDI conjugates. (cdc.gov)

In our group we design, synthesize and optimize new noncovalent binding motifs for biomacromolecules. (uni-due.de)

Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). (bvsalud.org)

The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)

The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. (ibecbarcelona.eu)
These synthetic polymers non-covalently bind to cargo, offering a mechanism to deliver proteins in a way that does not alter protein secondary structure. (umass.edu)

Notably, certain B. subtilis mutants with mutated Fp lack covalently bound flavin and do not assemble the SQR polypeptides in the membrane [3]. (lu.se)

The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)
Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)
The Nobel Prize-winning modification that prevents the innate immune system from recognizing injected mRNA as foreign and blocking transcription of the protein it encodes has been found on some occasions to cause ribosomal frameshifting. (bioworld.com)
It has been determined that VPS13A encodes for a protein called chorein. (medscape.com)

For OmpG we probed its potential to be a nanopore sensor for protein detection and quantification. (umass.edu)

As a result, its cytotoxicity appears to be related to the extent of its interstrand cross-linking with DNA, probably by binding at the N 7 position of guanine. (nih.gov)
Cisplatin is a platinum-containing compound that exerts an antineoplastic effect by covalently binding to DNA, with preferential binding to N-7 position of guanine and adenosine. (medscape.com)

The proteostasis network (PN) is an assembly of distinct dynamic molecular pathways that control the functionality of the proteome (proteome homeodynamics) during protein synthesis, folding, trafficking, and degradation. (hindawi.com)
Through molecular dynamics we have analysed the stability of the ISCA1-cryptochrome complex and characterized the interaction at the binding sites between individual cryptochrome and ISCA1. (nature.com)
One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)
The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
The proteins that make up the molecular machinery of life tends to fragment the research field into method-oriented have been perfected by several billion years of evolution subspecialties that rarely confront each other. (lu.se)
One contribution of 16 to a Discussion Meeting Issue `The molecular interact with a protein can, with little ambiguity, be classi- basis of life: is life possible without water? (lu.se)

The high mobility of hydration water ensures that all thermally activated processes at the protein-water interface, such as binding, recognition and catalysis, can proceed at high rates. (lu.se)

SN-38 binds to and stabilizes the topoisomerase I-DNA complex and prevents the relegation of DNA after it has been cleaved by topoisomerase I, inhibiting DNA replication. (medscape.com)

Cells express a pool of thousands of different proteins that need to be tightly controlled for proper cellular structure, organization, and function. (hindawi.com)
Like fly fishing, you keep casting to that position, never tightly binding to that position, but also never completely dissociated from that position," he says. (the-scientist.com)

They have been defined as monotropic integral membrane proteins located primarily in the endoplasmic reticulum (COX-1) and the perinuclear envelope (COX-2). (medscape.com)
Covalently bound radioactivity in the liver was associated primarily with proteins in the microsomal and 105,000 x g supernatant fractions. (aspetjournals.org)

The possible mechanism to explain the biophysics of this compass sense involves electron transfers within the photoreceptive protein cryptochrome. (nature.com)
The researchers found that the two proteins fluctuated between bound and loosely associated states, in support of the so-called fly-casting mechanism of protein-protein interaction. (the-scientist.com)
SUMOylation of proteins is an important mechanism in cellular responses to environmental stress ( 21 , 22 ). (frontiersin.org)

Keratin ( / ˈ k ɛr ə t ɪ n / [1] [2] ) is one of a family of structural fibrous proteins also known as scleroproteins . (wikipedia.org)
Spider silk is classified as keratin, [9] although production of the protein may have evolved independently of the process in vertebrates. (wikipedia.org)

Carbohydrates differ from proteins in two important features. (iucr.org)
After activation C3b can bind covalently, via its reactive thiolester, to cell surface carbohydrates or immune aggregates. (lu.se)

Crystallographic studies reveal the formation of a new pocket that is not apparent in previous structures of Ras, beneath the effector binding switch-II region. (rcsb.org)
A structural genomics approach is used to determine to the structures of proteins belonging to other classes of ABC transporters. (rug.nl)

The mitochondria have their own chaperones and proteolytic enzymes that remove damaged or unfolded proteins [ 18 - 20 ]. (hindawi.com)
For instance, many household cleaners use enzymes to speed up the breakdown of protein or starch stains on clothes. (newworldencyclopedia.org)

Large proteins are typically not suited for oral administration, as such drugs would be degraded in the digestive tract and poorly absorbed. (pharmtech.com)
Pore forming proteins are typically the proteins that form channels in membranes. (umass.edu)

This region, where the binding of the substrate(s) and the reaction occur, is known as the active site of the enzyme. (newworldencyclopedia.org)
There are two models to explain how the binding of enzyme and substrate occurs: the "lock and key" model and induced fit. (newworldencyclopedia.org)
This model of exact fit, introduced in the 1890s, is often referred to as the "lock and key" model, because the enzyme binding to a substrate is analogous to the specific fit of a lock into a key. (newworldencyclopedia.org)

Yes, the No-Stain Protein Labeling Reagent provides uniform labeling of proteins in gels or on membranes, provided they are imaged the same way. (thermofisher.com)
Can I get a similar signal intensity from two same-type gels or two same-type membranes that I label using the No-Stain Protein Labeling Reagent? (thermofisher.com)
Yes, reproducible signal intensity will be obtained when using the No-Stain Protein Labeling Reagent to label either the same type, identically loaded gels or the same type membranes from the transfer of identically loaded, same-type gels: quantitation results will be similar between the gel pairs and the membrane pairs. (thermofisher.com)

3 The tandem CBS domain 4 , which is linked to the nucleotide binding protein, plays a pivotal role in the regulation. (rug.nl)

The polymer complex consists of ISCA1 protein monomers with internally bound iron sulphur clusters and simultaneously binds ten cryptochromes. (nature.com)
The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)

However, correlations of the protein binding-delivery relationship or the role of polymer-protein complexation on intracellular activity of protein cargo are understudied. (umass.edu)
The work presented herein connects a fundamental understanding of polymer-protein complexation with intracellular internalization and cargo activity. (umass.edu)
Studies that facilitate understanding in CPPM-mediated intracellular protein delivery provide unprecedented insight for how non-covalently bound carriers deliver cargo. (umass.edu)

In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed. (ibecbarcelona.eu)
At present it offers more information about protein-protein interaction dynamics than can be obtained with conventional structural biology techniques such as nuclear magnetic resonance (NMR) and X-ray crystallography. (the-scientist.com)
and (ii) the perturbation progress within the field of protein hydration has been of the structure and dynamics of bulk water caused by the interaction with the solute. (lu.se)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center . (thermofisher.com)
Fluorophores for multiplexing that are compatible with the No-Stain Protein Labeling Reagent are listed here, under the Secondary Abs tab (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/total-protein-normalization.html). (thermofisher.com)

SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)

This work focuses on two E. coli pore forming proteins that have vastly differing roles in nature. (umass.edu)
SQR in E. coli and B. subtilis can be assembled in the membrane without covalently bound FAD but are enzymatically inactive [3, 4, 6]. (lu.se)
It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)
The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)

Heide van der T., and Poolman, B. (2002) ABC transporters: one, two or four extracytoplasmic substrate-binding sites? (rug.nl)
Their primary function is to shuttle intermediates between active sites via a covalently bound phosphopantetheine arm. (rsc.org)
Our results converge on allosteric sites that are conformationally coupled to the active-site WPD loop and are hotspots for fragment binding. (rcsb.org)
Porcine tubulin was incubated with profenofos and was then digested with trypsin, followed by mass spectrometric identification of the profenofos-modified tubulin and binding sites. (cdc.gov)

We have proposed that under conditions of severe osmotic stress (panel b, lower figure), the cell membrane contacts the nucleoid, which creates pools of proteins and hinders free diffusion through the cell. (rug.nl)

New modeling approaches reveal 'hidden' low-occupancy conformational states for protein and ligands. (rcsb.org)

They form one of the largest known protein families and are found in organisms from all kingdoms of life. (rug.nl)
SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin signaling pathway. (frontiersin.org)
It was also found that both nests share a common 77 KDa protein that has properties similar to those of the ovotransferrin protein in eggs. (researchgate.net)

In this review, we consider some of the theoretical aspects of protein haptenation, how mechanisms of protein haptenation can be investigated experimentally and how we can use such knowledge in the development of novel, alternative approaches for predicting skin sensitization potential in the future. (cdc.gov)

Biotheryx Inc. has developed new proteolysis targeting chimeras (PROTACs) comprising a E3 ubiquitin ligase binding moiety covalently bonded to a CDK protein-targeting moiety through a linker. (bioworld.com)

Acyl carrier proteins are critical components of fatty acid and polyketide biosynthesis. (rsc.org)
Selectivity for the holo over apo -form of acyl carrier proteins is demonstrated indicating that only the phosphopantetheine - thiol is modified. (rsc.org)

These main PG groups are further classified into families according to their gene homology, core protein properties, size, and modular composition. (hindawi.com)
During this evolutionary process, the experimental basis of the current (multiple) views on proteins have adapted to and exploited the unique physi- protein hydration, with an emphasis on the dynamic cal properties of liquid water (Eisenberg & Kauzmann aspects. (lu.se)

Outer membrane protein G (OmpG) is an innocuous β-barrel porin while Cytolysin A (ClyA) is an α-helical pore forming toxin. (umass.edu)
These components are the Haemophilus influenzae type b capsular polysaccharide [polyribosylribitol phosphate (PRP)] that is covalently bound to an outer membrane protein complex (OMPC) of Neisseria meningitidis and hepatitis B surface antigen (HBsAg) from recombinant yeast cultures. (theodora.com)

Anatomy9

Organisms17

Chemicals and Drugs135

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes53

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science3

Health Care3

Geographicals2

Molecules20

Interactions9

Peptides3

Irreversibly2

Ligand4

Residues4

Polypeptide6

Serum7

Complexes5

Compounds4

Fluorescence7

Lipids3

Cysteine3

Nucleic acids1

Biotin2

Soluble1

Target proteins2

Covalent4

Tissue Proteins1

Cytochrome1

Inhibitors2

Antibodies2

Complement2

Chains1

Phosphorylation1

Glycan1

Antibody1

Affinity1

Cytoplasm1

Transcriptional1

Mutations1

Peptide4

Albumin2

Noncovalent1

Receptor1

Genes1

Synthetic2

Polypeptides1

Encodes4

Quantification1

Position of guanine2

Molecular6

Catalysis1

Replication1

Tightly2

Primarily2

Mechanism3

Keratin2

Carbohydrates2

Structures2

Enzymes2

Typically2

Enzyme3

Membranes3

Regulation1

Clusters2

Intracellular3

Interaction3

Assays2

Structural1

Coli4

Sites4

Cell membrane1

Conformational1

Found3

Approaches1

Linker1

Carrier2

Properties2

Outer2