• Counterstains stain cell nuclei and are packaged as convenient, ready-to-use solutions for use on slides or in staining dishes. (2bscientific.com)
  • Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. (sigmaaldrich.com)
  • Product Description google The Biosensis AG-400-AG kit utilizes an ethidium bromide counter stain for a quick and effective way to visualize cell nuclei and cell bodies of cells while under UV illumination allowing the assessment of amyloid plaques and cell/tissue positioning as well in one step. (biosensis.com)
  • EtBr counter stain stains nuclei and cell bodies for easy identification and spacial orientation. (biosensis.com)
  • Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
  • The nuclei were counterstained with Hoechst 33258. (fsu.edu)
  • The nuclei of cells samples from the endometrium, appendix, are stained by its active product, haematin, gall bladder, spleen and other gastric and which is produced when an oxidizing agent pleural tissues obtained by surgery, curet- is added. (who.int)
  • Five sections (5 µm thick) ity to stain nuclei without combining with a were prepared from each paraffin block mordant, such as aluminium, iron or other and placed on different slides. (who.int)
  • In Gram staining, crystal violet stains only Gram-positive bacteria, and safranin counterstain is applied which stains all cells, allowing the identification of Gram-negative bacteria as well. (wikipedia.org)
  • A secondary counterstain such as safranin is applied which stains the decolorized Gram negative cells pink or red. (colorwithleo.com)
  • The composition of the cell wall of a microorganism determines whether it will retain crystal violet dye or be decolorized and made visible only with the counterstain, safranin. (who.int)
  • Those organisms that retain the crystal violet dye will stain blue, those that do not are stained red with safranin. (who.int)
  • What is the conclusion of the Gram stain? (colorwithleo.com)
  • The Gram stain is a differential staining technique used to classify bacteria into two large groups based on the physical properties of their cell walls. (colorwithleo.com)
  • The Gram stain process involves staining bacteria with crystal violet dye, then treating with an iodine solution, followed by a decolorizer, and finally a counterstain. (colorwithleo.com)
  • Based on their cell wall structure, bacteria will either retain the crystal violet dye (Gram positive) or lose the crystal violet dye and take on the color of the counterstain (Gram negative). (colorwithleo.com)
  • The conclusion of the Gram stain provides information about the broad classification of bacterial species which can aid in diagnosis and treatment of bacterial infections. (colorwithleo.com)
  • How does the Gram stain work? (colorwithleo.com)
  • The Gram stain relies on the different properties of Gram positive and Gram negative cell walls. (colorwithleo.com)
  • The Gram stain provides immediate, useful information to clinicians about unknown bacterial samples. (colorwithleo.com)
  • The Gram stain provides a rapid, inexpensive method to categorize bacteria as Gram positive or Gram negative based on properties of their cell wall structure. (colorwithleo.com)
  • The conclusion of the Gram stain gives clinicians key preliminary information about potential pathogens to help guide diagnosis and empiric treatment while definitive culture results are pending. (colorwithleo.com)
  • Gram staining technique is used for staining bacteria, yeasts and aerobic actinomycetes. (who.int)
  • Gram stains can be made from blood culture and solid culture. (who.int)
  • Record the QC results on the Gram stain QC sheet provided in Annex 1. (who.int)
  • Gram stain is the most common stain used in microbiology to identify bacteria. (medscape.com)
  • The appearance of organisms that stain gram-positive or -negative means that the smear contains organisms. (medscape.com)
  • A variation of Gram staining is called Atkin Gram stain, in which the primary stain is gentian violet and mordant is Atkin iodine. (medscape.com)
  • Gram-negative organisms take the counterstain and stain pink/red. (medscape.com)
  • Gram stain is used to identify the organism causing a disease or pathological condition. (medscape.com)
  • Organisms without a cell wall (eg, Mycoplasma species) and small bacteria (eg, Chlamydia and Rickettsia species) do not stain with Gram stain. (medscape.com)
  • Gram stain is the most common stain for general bacterial identification. (msdmanuals.com)
  • Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. (biosensis.com)
  • If still unacceptable, check the quality of the reagent, check if the staining procedure was followed correctly and check if the correct reference strains were used. (who.int)
  • This IHC protocol provides a basic guide for the fixation, microtome sectioning, and staining of paraffin-embedded tissue samples. (rndsystems.com)
  • For example some fixation methods destroy epitopes of certain proteins and therefore do not allow antibody staining afterwards. (neb.com)
  • Use the Alcian Blue (pH 2.5) Stain Kit to visulaize sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins). (2bscientific.com)
  • Alcian blue pH 2.5 - for the detection of acidic mucosubstances - for microscopy, is a ready-to-use staining kit used to stain acidic mucopolysaccharides in human-histological specimen materials, for example histological sections of e. g. the intestine or stomach. (sigmaaldrich.com)
  • An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. (sigmaaldrich.com)
  • To study the effect of tap water `softening' (i.e. calcium extraction) on H&E stains, 5 sets of slides from 30 different paraffin-embedded human pathologic tissue blocks were prepared in the same way except for washing with 5 different types of water. (who.int)
  • Soft tissues were dissected out and frozen sectioned en bloc into 15 micrometer longitudinal sections, while bones were paraffin embedded and cut into 5 micrometer sections, before mounting on coated slides. (cdc.gov)
  • Tissue sections on slides were analyzed for the presence of calcium salts using von Kossa staining. (cdc.gov)
  • Tray for staining slides. (medscape.com)
  • DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. (sigmaaldrich.com)
  • This mordant is more effective in retaining the primary stain. (medscape.com)
  • Is there a product or procedure for removing tough stains from granite? (hubpages.com)
  • Counterstains are often applied haphazardly at the end of an IHC procedure with little thought given to optimizing conditions for providing the best contrast with the specific antigen staining. (2bscientific.com)
  • Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain. (wikipedia.org)
  • ABSTRACT Haematoxylin and eosin (H&E) is the most popular routine stain used in pathology laboratories for highlighting cellular structures. (who.int)
  • They are also sold with rubber feet on the bottom, giving the shelf a bit of a raise from your counter while protecting it from scratches and bacteria build up. (barsupplies.com)
  • These make stain less susceptible to decolorization. (medscape.com)
  • Stain solution for demonstrating acid mucopolysaccharides. (mcssl.com)
  • Cells can be counterstained with any live-cell dye that is compatible with the fluorescent properties of the CoA substrate for simultaneous microscopic detection. (neb.com)
  • We routinely add 5 µM Hoechst 33342 to the medium prior to the first wash (Step 3) as a DNA counterstain and leave this on the cells for 2 minutes prior to completing the wash steps. (neb.com)
  • To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
  • The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
  • When cells are stained with crystal violet, crystal violet binds to the cell wall. (medscape.com)
  • 10% HER2 expression is deemed negative as long as the result of immunohistochemical staining is weak and incomplete or the result of in situ hybridization (ISH) is negative. (medscape.com)
  • Application Details Staining of amyloid plaques in human and animal tissues, see included protocol. (biosensis.com)
  • Consistent quality dye makes a stable, self-differentiating solution for staining amyloid. (mcssl.com)
  • How do you remove rust stains from a Stainless Steel sink? (hubpages.com)
  • No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. (bvsalud.org)
  • For the measurements of area and intensity in total and stained region, the average mean of difference in percentage between SQ and ImageJ were all less than 0.05. (bvsalud.org)
  • Results: Discrete sites of von Kossa staining were present in forelimb tendons at the level of the wrist in week 6 and 12 HRLF rats, but not in controls. (cdc.gov)
  • Von Kossa staining was greatest in HRLF week 12 forelimb tendons in matrix regions surrounding tendon fi broblasts. (cdc.gov)
  • Supraspinatus tendons were also examined, but no von Kossa staining was present at any time point in either exposure group. (cdc.gov)
  • The algorithm was developed in R4.0.2 with over a thousand in-house histological images containing Alizarin Red (AR) and Von Kossa (VK) staining , and was validated using external images. (bvsalud.org)
  • Although the causes of non-specific background staining in immunohistochemistry (IHC) and immunofluorescence (IF) applications may differ slightly, they can obscure the detection of your specific signal. (2bscientific.com)
  • A decolorizing agent such as ethanol or acetone is used to wash away excess stain. (colorwithleo.com)
  • Removes excess stain from background for clean, crisp nuclear staining. (mcssl.com)
  • Dilution: 1:100 using IHC-Tek TM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. (ihcworld.com)
  • A similar picture emerged for the antibody anti-mOR37, a small number of glomeruli in the ventral domain of the bulb was stained. (jneurosci.org)
  • Acidic mucosubstances are selectively stained in the tissue, after which the specimen is counterstained with nuclear fast red. (sigmaaldrich.com)
  • In the case of CD24 staining, there was no significant difference between NPC and chronic nasopharyngitis tissues. (hindawi.com)
  • Rapid, brilliant counterstain that produces multi-hued effects on properly fixed tissues. (mcssl.com)
  • With a uniquely flexible formula, theBalmJour starts as a gloss, finishes as a stain, and delivers the high-pigment payoff of a lipstick. (mycosmeticcounter.com)
  • theBalmJour Lip Gloss Stains help you up your gloss game for a perfect mix of stain and shine! (mycosmeticcounter.com)
  • DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. (sigmaaldrich.com)
  • Fluorochrome stains (eg, auramine-rhodamine) also identify acid-fast organisms, but a special fluorescent microscope is required. (msdmanuals.com)
  • Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups. (bvsalud.org)
  • Development of an image processing software for quantification of histological calcification staining images. (bvsalud.org)
  • Quantification of the histological staining images gives important insights in biomedical research . (bvsalud.org)
  • In wet lab, it is common to have some stains off the target to become unwanted noisy stains during the generation of histological staining images. (bvsalud.org)
  • Our results indicated that the SQ tool is well established for automatic histological staining image quantification. (bvsalud.org)
  • In this protocol, signal visualization is achieved using R&D Systems' Cell and Tissue Staining Kits . (rndsystems.com)
  • Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (abcam.com)
  • Counter Caddies™ - STAINED Finish - 1. (barsupplies.com)
  • Our trademarked Counter Caddies™ in a gingerbread Christmas theme is designed to accommodate the baker who favors organization in their kitchen. (barproducts.com)
  • Our trademarked Counter Caddies™ are designed to accommodate the chef who favors organization in their kitchen. (barproducts.com)
  • Our Customizable Monogram LED Counter Caddies™ make for a great gift! (barproducts.com)
  • We updated our popular Counter Caddies™ by adding LED lighting! (barproducts.com)
  • The beautiful ready-to-use stain made with our high quality EZ Carmine dye that allows for decreased staining time. (mcssl.com)
  • Counterstains are sometimes used to separate animals from organic detritus in microbiology studies. (wikipedia.org)
  • Haematoxylin stain is extracted from formalin, were processed routinely for his- the logwood of a tropical tree, Haematoxy- topathology. (who.int)
  • 4 solved in soft water, used as a counterstain to haematoxylin. (who.int)
  • A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope. (wikipedia.org)
  • After staining, the slide is examined under microscope with oil immersion. (medscape.com)
  • Legionella species do not stain but are visualized from growth in a culture medium. (medscape.com)
  • R&D Systems offers VisUCyte™ HRP Polymer secondary antibodies and staining kits to provide cleaner and faster results. (rndsystems.com)
  • The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. (cellsignal.com)
  • Introducing a simple and fun way to choose your Counter Caddy design from several pre-made pattern templates. (barproducts.com)
  • Similarly, more intense staining of CD44v6 and ALDH1A1 was detected in an NPC cell line than in an immortalized nasopharyngeal epithelial cell line. (hindawi.com)
  • Sections with tendons were stained in 3% silver nitrate solution for 1.5 hours under intense light, rinsed in distilled water, 5% sodium thiosulfate for 2 minutes, and counterstained with nuclear red. (cdc.gov)
  • Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. (sigmaaldrich.com)
  • Longer incubation times tend to result in stronger background staining and do not necessarily increase the signal to background ratio. (neb.com)
  • Learn about the sources of background staining and how to troubleshoot unwanted background in IHC and IF applications. (2bscientific.com)
  • instead, the stained region is identified based on assumptions that the background is pure and clean. (bvsalud.org)
  • 10% that is weak and incomplete and negative on ISH should be classified as HER2 low, while staining ≤10% should be classified as HER2 negative. (medscape.com)
  • Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. (biosensis.com)
  • Low calcium and magnesium ions and high sodium ions in soft water adversely affect the results of routine H&E stain. (who.int)
  • Ziehl-Neelsen and Kinyoun stains are acid-fast stains used to identify mainly mycobacteria, particularly M. tuberculosis . (msdmanuals.com)
  • This trademarked counter caddy is designed to accommodate the home coffee connoisseur. (barsupplies.com)
  • Make your morning routine more organized and breeze through making your delightful java concoctions with this beautiful counter caddy. (barsupplies.com)