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  • genome
  • I am looking for a cosmid library of A. tumefaciens genome. (bio.net)
  • Cosmids were provided by the European Drosophila Genome Project (EDGP). (fruitfly.org)
  • Two independent sets (A and B) of five cosmids were identified whose inserts overlap and represent the entire viral genome, and set C was obtained by replacing two cosmids in set B. Each set gave rise to viral plaques when digested with PacI and transfected into cells in culture. (nih.gov)
  • Five overlapping type 1 Epstein-Barr virus (EBV) DNA fragments constituting a complete replication- and transformation-competent genome were cloned into cosmids and transfected together into P3HR-1 cells, along with a plasmid encoding the Z immediate-early activator of EBV replication. (asm.org)
  • fragments
  • Epstein-Barr virus recombinants from overlapping cosmid fragments. (asm.org)
  • These recombinants probably result from recombination among the transfected cosmid-cloned EBV DNA fragments. (asm.org)
  • Of the recombinants which had incorporated the marker-rescuing cosmid DNA fragment and the fragment encoding the type 1 EBNA 3A gene, most had incorporated markers from at least two other transfected cosmid DNA fragments, indicating a propensity for multiple homologous recombinations. (asm.org)
  • overlap
  • Two cosmids common to sets B and C ostensibly contain one of the origins of viral DNA replication (oriL) in a region of overlap between inserts, but both actually consist of a minority of apparently intact (ori+L) forms and a majority of deleted (ori-L) forms. (nih.gov)
  • deletion
  • P3HR-1 cells harbor a type 2 EBV which is unable to transform primary B lymphocytes because of a deletion of DNA encoding EBNA LP and EBNA 2, but the P3HR-1 EBV can provide replication functions in trans and can recombine with the transfected cosmids. (asm.org)
  • markers
  • Ferretti, L. 2009-03-24 00:00:00 Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for the presence of microsatellite markers and physically mapped to chromosomes by FISH. (deepdyve.com)
  • prepare
  • This can be easily achieved by saving 1-2 mL liquid cell culture when you first prepare the cosmid DNA from freshly streaked plate. (bio.net)
  • After you have checked and know that the cosmid DNA is good, spin down the cells, discard the supernatant and resuspend the pellet in 1 mL sterile 20% glycerol solution (better to prepare two microtubes of the cell stock). (bio.net)
  • cells
  • cells and we can make good cosmid DNA from them. (bio.net)
  • Are the cosmid-containing cells less viable? (bio.net)
  • Overall, the type 1 EBNA 3A gene was incorporated into 26% of the transformation marker-rescued recombinants, a frequency which was considerably higher than that observed in previous experiments with two-cosmid EBV DNA cotransfections into P3HR-1 cells (B. Tomkinson and E. Kieff, J. Virol. (asm.org)
  • either
  • Cosmids are available either singly or on high-density filters. (fruitfly.org)
  • When either of these cosmids was replaced by a derivative comprising only ori-L forms, ori+L and ori-L progeny were obtained, and only ori-L progeny were produced when both were replaced. (nih.gov)
  • Viral mutants with lesions in either or both of genes UL2 and UL44, which are not essential for growth in cell culture, were constructed using cosmids containing specifically introduced frameshift mutations. (nih.gov)
  • near
  • The frequency of incorporation of the nonselected transfected type 1 EBNA 3C gene, which is near the end of two of the transfected cosmids, was 26% overall, versus 3% in previous experiments using transfections with two EBV DNA cosmids. (asm.org)
  • contains
  • One cosmid in set A contains the oriL locus in a nonoverlapping region and lacks ori+L forms. (nih.gov)
  • type
  • A cosmid-based system for constructing mutants of herpes simplex virus type 1. (nih.gov)
  • EBV recombinants which have the type 1 EBNA LP and 2 genes from the transfected EcoRI-A cosmid DNA were selectively and clonally recovered by exploiting the unique ability of the recombinants to transform primary B lymphocytes into lymphoblastoid cell lines. (asm.org)
  • The one recombinant virus examined in detail by Southern blot analysis has all the polymorphisms characteristic of the transfected type 1 cosmid DNA and none characteristic of the type 2 P3HR-1 EBV DNA. (asm.org)