Many different restriction fragments derived from these two contigs were found to be evolutionarily conserved and hybridized to distinct message transcripts. (elsevierpure.com)
One cosmid, ICRFc104C03.184, identified potential junctional-fragments in several restriction digests of AnLy hybrid DNA. (ox.ac.uk)
Twenty-four of these clones were purified, analyzed, and found to comprise two groups according to the reactivity of the recombinant proteins with LL sera and to the DNA restriction patterns of the recombinant plasmids and cosmids. (elsevierpure.com)
Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
Genomic4
A similar analysis with DNA fragments derived from these two genomic regions also failed to detect any alterations. (elsevierpure.com)
Cosmids of genomic libraries from M. smegmatis and Mycobacterium bovis BCG were screened for complementation of the mutation. (elsevierpure.com)
The patient-derived material included genomic DNA from an EDA male (EDA family 1015) with an interstitial deletion, and a cell line GM0705A, obtained from an isolated female patient with a de novo balanced (X;9) translocation, and the somatic hybrid, AnLy, derived from this cell line. (ox.ac.uk)
In this genomic stretch of DNA we have identified sequence homologous to the U7 RNA gene including its potential regulatory elements. (wustl.edu)
Recombinant2
The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
What was the first recombinant DNA based product, produced and marketed in India? (mcqexams.com)
Plasmids1
Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
Chromosomal2
This was confirmed by FISH analysis of the GM0705A cell line with total cosmid ICRFc104C03.184, in which both chromosomal elements of the (X;9) translocation were identified. (ox.ac.uk)
Sequences homologous to the A15 gene were detected in chromosomal DNA from Mycobacterium avium, Mycobacterium bovis BCG, and M. tuberculosis. (elsevierpure.com)
Genes5
Consistent loss of DNA sequences from several regions on the short arm of human chromosome 3 has suggested that multiple tumor suppressor genes reside on chromosome 3p in various types of cancer cells. (elsevierpure.com)
We have identified five new genes from these two contigs and there is evidence to suggest that several additional genes reside within these cosmid contigs. (elsevierpure.com)
The genes identified from 3p21.1 were then hybridized to DNA, isolated from a series of lung cancer cell lines and matched normal and tumor DNA from lung cancer patients. (elsevierpure.com)
Sequencing of the DNA revealed four open reading frames whose genes were named fxuA. (elsevierpure.com)
To contribute to the identification and analysis of novel genes, we undertook the study of a cosmid clone in the Xq27 region of human DNA. (wustl.edu)
Clones4
Starting with two cosmids within 3p21.1, D3S92 and D3S93, we have isolated two separate contigs of overlapping cosmids within 3p21.1, by screening a library of 5700 chromosome 3-specific cosmid clones. (elsevierpure.com)
DNA probes from DXS732 and DXS453, identified as the closest flanking marker loci to the EDA locus, were used to identify homologous Yeast Artificial Chromosome (YAC) clones. (ox.ac.uk)
These two YACs were also screened against the ICRF human X chromosome cosmid library and identified 36 cosmid clones. (ox.ac.uk)
Direct cosmid-cosmid hybridisation analysis placed subsets of these clones within four different cosmid contigs. (ox.ac.uk)
Chromosome1
YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
Proteins5
Screening of the Mycobacterium leprae cosmid library with pooled sera from lepromatous leprosy (LL) patients by a colony immunoblot technique resulted in the identification of about 100 colonies that produced immunologically reactive proteins. (elsevierpure.com)
Proteins were known to be composed of 20 distinct amino acids whereas DNA is composed of only 4 nucleotides. (proprofs.com)
Different proteins were known to have unique sequences, whereas it was thought that all DNA molecules have the same sequence. (proprofs.com)
In the early twentieth century, it was believed that proteins might carry genetic information because proteins were known to be composed of 20 distinct amino acids, whereas DNA is composed of only 4 nucleotides. (proprofs.com)
DNA, RNA and proteins can be separated using this technique. (mcqexams.com)
Clone2
A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. (biologyexams4u.com)
It is a single standard DNA or RNA tagged with a radioactive molecule, which is complementary to the DNA in a clone of cells. (mcqexams.com)
Homologous1
These fragments were used to identify homologous cDNAs from an adenogastric cDNA library, and several of these cDNAs were partially sequenced. (elsevierpure.com)
Isolation1
1994. Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture . (wisc.edu)
Sequence2
Sequence analysis of a 1.2-kb fragment containing the entire coding sequence of A15 revealed three open reading frames (ORFs), only one of which (ORF II) contains sufficient genetic information to encode for A15. (elsevierpure.com)
1996. Mutation detection by cleavase fragment length polymorphism . (wisc.edu)
Evolutionarily conserved1
The cloned fragment was previously observed to have a high number of evolutionarily conserved sequences. (wustl.edu)
Molecule2
a) The cell must be made competent in biotechnology experiments because DNA being a hydrophilic molecule, cannot pass through cell membrane. (mcqexams.com)
Hence, the cell should be made competent to accept the DNA molecule. (mcqexams.com)
Molecular1
Characterisation of molecular DNA rearrangements within the Xq12-q13.1 region, in three patients with X-linked hypohidrotic ectodermal dysplasia (EDA). (ox.ac.uk)
Gene1
Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. (mcqexams.com)
Vectors1
Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
Probes1
No alterations were detected with any of these probes, both at the DNA or RNA levels. (elsevierpure.com)
Primers1
Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand at 54$$^0$$C. (mcqexams.com)
Copies1
It is responsible for forming new copies of DNA from nucleic acids. (mcqexams.com)
Cells4
The flow of genetic information in cells is that DNA is first transcribed into RNA through a process called transcription. (proprofs.com)
These cells are subjected to hypotonic lysis and individual chromosomes or fragments are isolated. (mcqexams.com)
The fragments of the chromosomes are incubated with whole cells/ eggs for transfection. (mcqexams.com)
In this method, microscopic particles of gold / tungsten are coated with the DNA of interest and bombarded onto the cells. (mcqexams.com)
Identification1
1994. Statement on use of DNA testing for presymptomatic identification of cancer risk . (wisc.edu)
Unique1
DNAs from cosmids of group II all contain a 10-kb PstI fragment that hybridized to the unique repetitive M. leprae DNA. (elsevierpure.com)
Individual1
Which type of bonds link the individual nucleotides together in DNA? (proprofs.com)
A single-copy probe pC03.184E2, derived from this cosmid, also identified the der(9)-derived junctional fragment when hybridised against AnLy DNA. (ox.ac.uk)
Important1
Calcium ions play an important role in neutralizing the natural negative charges possessed by both DNA and cell surface to avoid electrostatic repulsion between the two. (mcqexams.com)