• Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. (up.ac.za)
  • Analysis of plasmid, cosmid or genomic DNA in combination with various methods, e.g. (openwetware.org)
  • Highly repetitive regions are unstable in plasmid/cosmids/BACs so their length and sequences couldn't be accurately determined. (blogspot.com)
  • First artificial cloning vector (1977) constructed by Boliver & Rodriguez from E .coli plasmid. (biologyexams4u.com)
  • Cosmids are the novel cloning vectors which possess properties of both plasmid and phage. (biologyexams4u.com)
  • 16 Selection for the active X chromosome yielded 12 clones bearing only the normal X and five clones retaining both X chromosomes. (bmj.com)
  • Back selection for the inactive X on one diploid clone yielded 12 hybrids containing the deleted inactive X chromosome, three of which were selected for analysis. (bmj.com)
  • Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)
  • Die Analyse großer Cosmid- und BAC- (Bacterial Artificial Chromosome) Contigs, welche im Rahmen dieser Arbeit erstellt wurden und die mehr als 1 Mb sowohl des X- als auch des Y-Chromosoms des Platys X. maculatus abdecken, zeigte eine hohe Dichte von Retroelementen und anderen repetitiven Sequenzen, besonders im Bereich des dominanten Onkogens Xmrk und in einer durch das duplizierte Gen ps-criptY gekennzeichneten, Y-spezifischen Region. (uni-wuerzburg.de)
  • YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
  • DNA probes from DXS732 and DXS453, identified as the closest flanking marker loci to the EDA locus, were used to identify homologous Yeast Artificial Chromosome (YAC) clones. (ox.ac.uk)
  • These two YACs were also screened against the ICRF human X chromosome cosmid library and identified 36 cosmid clones. (ox.ac.uk)
  • This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). (tamu.edu)
  • cDNA Cloning and expression of the plastidic copper/zink - superoxide dismutase from spinach (Spinacea vleracea L.) leaves. (scielo.org.za)
  • This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
  • Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
  • We have cloned cosmids containing the trichothecene gene cluster from Fusarium graminearum F15 (i.e. (usda.gov)
  • Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
  • Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
  • Genomic DNA clone containing exons 2-8 for hMSH3 gene. (riken.jp)
  • Name an eukaryotic organism that has plasmids, and can be used as a host in gene cloning experiments. (mcqexams.com)
  • The gene that encodes the CREB binding protein, or CBP (also called CREBBP ), was cloned in 1995, and mutations in this gene have been found in some patients with Rubinstein-Taybi syndrome. (medscape.com)
  • Therefore, the use of 5 cosmids covering the entire gene for fluorescence in situ hybridization (FISH) in order to identify more proximal deletions is recommended. (medscape.com)
  • One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K origin. (addgene.org)
  • Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
  • The cloned genes in these vectors are not expected to express themselves at the transcription or translation level. (biologyexams4u.com)
  • Cloning vectors (Cloning vehicles) are used for creating genomic libraries, preparing probes, genetic engineering, or other basic studies. (biologyexams4u.com)
  • The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
  • Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
  • So that it features which are useful for molecular cloning experiments. (biologyexams4u.com)
  • Twenty-four of these clones were purified, analyzed, and found to comprise two groups according to the reactivity of the recombinant proteins with LL sera and to the DNA restriction patterns of the recombinant plasmids and cosmids. (elsevierpure.com)
  • Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. (wikipedia.org)
  • Recent years have also brought the first successes in molecularly cloning genes of developmental interest defined only by mutation, using transposon tagging as a generally applicable method for identification of the desired DNA sequences. (cshlpress.com)
  • A physical map of the genome has been assembled with a combination of cosmid and YAC genes. (cshlpress.com)
  • Finally, we succeeded in cloning of the genomic deoxyribonucleic acid (DNA) which contains genes encoding melanoma antigen with species specific determinant (M562). (go.jp)
  • Characterization (polymerase chain reaction, nucleic acid sequencing) and mutagenisis of cloned DNA fragments. (up.ac.za)
  • A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. (biologyexams4u.com)
  • One cosmid, ICRFc104C03.184, identified potential junctional-fragments in several restriction digests of AnLy hybrid DNA. (ox.ac.uk)
  • This will help make sense of the restriction pattern when run on an agarose gel and/or isolating a specific fragment for sub-cloning. (openwetware.org)
  • Should have unique restriction sites as polylinkers or multiple cloning site (MCS). (biologyexams4u.com)
  • The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning. (qiagen.com)
  • A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid. (tamu.edu)
  • DNAs from cosmids of group II all contain a 10-kb PstI fragment that hybridized to the unique repetitive M. leprae DNA. (elsevierpure.com)
  • The B16-melanoma DNA cosmid libraries were constructed with the shuttle vector pCV103. (go.jp)
  • This was confirmed by FISH analysis of the GM0705A cell line with total cosmid ICRFc104C03.184, in which both chromosomal elements of the (X;9) translocation were identified. (ox.ac.uk)
  • Reintroduction of cloned DNA sequences into the genome has recently been accomplished. (cshlpress.com)
  • We investigated the roles of storage and parsing in the visual domain for the productive Dutch plural suffix -en.Two experiments are reported that show that storage occurs for high-frequency noun plurals. (mpi.nl)
  • Direct cosmid-cosmid hybridisation analysis placed subsets of these clones within four different cosmid contigs. (ox.ac.uk)
  • Mapping of anchor clones from each contig, against the mapping panel, localised all these contigs within the Xq12-q13.1 region. (ox.ac.uk)
  • It is a single standard DNA or RNA tagged with a radioactive molecule, which is complementary to the DNA in a clone of cells. (mcqexams.com)
  • It is a 4362 kb long widely used cloning vector. (biologyexams4u.com)
  • The first widely used Cloning vector $$pBR322$$ that contains around 4300 base pairs. (mcqexams.com)
  • BAC-Optimized Replicator Cells are components of Lucigen's CopyRight®Cloning Kits . (bjzblg.com)
  • The first competent cells developed by Lucigen specifically for large DNA cloning. (bjzblg.com)
  • M. leprae clones previously isolated in our laboratory by immunological screening with LL patients' sera. (elsevierpure.com)
  • pathogens with the closely related human pathogen, en- teropathogenic E. coli ( 4 , 5 ). (cdc.gov)
  • The human MSH3 genomic DNA clone f9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. (riken.jp)
  • Screening of the Mycobacterium leprae cosmid library with pooled sera from lepromatous leprosy (LL) patients by a colony immunoblot technique resulted in the identification of about 100 colonies that produced immunologically reactive proteins. (elsevierpure.com)
  • Proteins specified by clones from group I reacted strongly with LL patients' sera on a Western blot (immunoblot), demonstrating a 15-kDa protein band designated A15. (elsevierpure.com)
  • Clones from group II did not show any reactive protein band on a Western blot, when reacted with patients' sera. (elsevierpure.com)
  • One of the λgt11 clones (L8) expresses a β-galactosidase fusion protein with 89 amino acids from the C terminus of A15. (elsevierpure.com)
  • Total DNA was isolated from the transfectants, and the B16 genomic DNA clones were rescued by the in vitro packaging with lysogenic bacterial extracts. (go.jp)
  • Parsing for noun plurals appears to be a time-costly process, which we attribute to the ambiguity of -en,a suffix that is predominantly used as a verbal ending. (mpi.nl)
  • Which cloning vector was discovered for the first time? (mcqexams.com)