• Cells from these lines are also often transfected to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. (wikipedia.org)
  • Hello All, What is the best way to extract expressed proteins from cos cells? (bio.net)
  • In primate cells, T-antigen usually controls SV40 DNA replication as well as the packaging of the virus into capsids, but in many other cells the protein binds to p53 and Rb proteins, which are important in the regulation of growth, essentially blocking their normal processes and resulting in the development of tumors. (fsu.edu)
  • Western blot and confocal microscopy analyses indicated that the total pool of hSVCT1 or hSVCT2 proteins expressed in the transfected COS-1 cells remained unaffected by PMA treatment. (physiology.org)
  • Further studies are required to clarify the nature of this inhibition, but the COS-1 expression system appears to be a useful tool for the possible identification of important regulatory proteins. (kent.ac.uk)
  • Both proteins localize in the photoreceptor connecting cilium (CC), a thin bridge linking the cell body and the light-sensing outer segment. (pnas.org)
  • Surprisingly, in both COS-7 cells and hippocampal neurons, GKAP forms insoluble aggregates with Shank that colocalize with heat shock protein 70 and neurofilaments, two markers of the aggresomes in which misfolded proteins accumulate. (jneurosci.org)
  • However, the two proteins are organized in clusters in COS cells and synaptic clusters in neurons when both are overexpressed and associated with wild-type PSD-95, but not with palmitoylation-deficient PSD-95. (jneurosci.org)
  • The mutant apo A-I mRNA had a size similar to that of the normal mRNA, and both mutant and wild-type apo A-I proteins were secreted into the cell media. (ahajournals.org)
  • We used green flourescent protein-tagged SG-associated RNA-binding proteins (specifically, TIA-1 and poly[A] binding protein [PABP-I]) to monitor SG assembly, disassembly, and turnover in live cells. (rupress.org)
  • Cell interaction with adhesive proteins or growth factors in the extracellular matrix initiates Ras/ mitogen-activated protein (MAP) kinase signaling. (rupress.org)
  • Inhibition of MAP kinase activity causes decreased MLCK function, MLC phosphorylation, and cell migration on extracellular matrix proteins. (rupress.org)
  • To obtain further insight into the Rh complex, we chose nonerythroid COS-1 cells instead of proerythroblast-like K562 cells, which produce endogenous Rh proteins as cell host, for the expression of both RhAG and RhD. (elsevier.com)
  • Human BMP-2 proteins are produced by culturing a cell transformed with a cDNA substantially as shown in Table II or Table III and recovering from the culture medium a protein containing the 97 amino acid sequence #299 to #396 of Table II or amino acid #311 to #408 of Table III, respectively. (google.com)
  • A growing family of regulatory proteins that deliver signals between cells of the immune system has been identified. (freepatentsonline.com)
  • Simultaneous expression of both proteins has also been observed in HeLa cells, but in both proliferating and confluent cells. (aacrjournals.org)
  • Transfection of HEK-293 cells with Mdm2 siRNA qualified prospects to 98% knockdown of Mdm2 proteins (Fig. 3and and 0.01, control versus USP33, 2-method ANOVA. (biologycu.org)
  • In eukaryotic cells, only time consuming methods exist to study the topology of membrane proteins. (mdc-berlin.de)
  • The fusion proteins were expressed in E.coli and COS.M6 cells and immunoblot analyses and enzyme activity assays were performed to localize the protein domains in both cell types. (mdc-berlin.de)
  • Therefore, LacZ fusions can localize cytoplasmic loops in COS.M6 cells by means of a simple enzymatic assay with high reliability and may be used in future studies to develop topological models of other eukaryotic membrane proteins in their authentic cell systems. (mdc-berlin.de)
  • It is also often used to transfect cells to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. (signagen.com)
  • The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells. (neb.com)
  • Characterization of the domains of hsp90 required to bind eNOS, using yeast 2-hybrid, cell-based coprecipitation experiments, and GST-fusion proteins, revealed that the M region of hsp90 interacts with the amino terminus of eNOS and Akt. (ahajournals.org)
  • Assigning functions to the vast array of proteins present in eukaryotic cells remains challenging. (nature.com)
  • The In-Cell Western™ Assay is an immunocytochemical assay that uses near-infrared fluorescence to detect and quantify proteins in fixed cells. (licor.com)
  • Proteins in fixed, cultured cells are detected directly in microplates, which yields higher throughput compared to Western blotting and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer. (licor.com)
  • In this cell type, protein 4.1R stabilizes the spectrin-actin network and mediates the attachment of the underlying cytoskeleton to the overlaying lipid bilayer through interactions with lipids (phosphatidylserine and phosphatidylinositol 4,5-bisphosphate) [ 1 , 2 ] as well as with integral membrane proteins [ 3 ]. (portlandpress.com)
  • Subsequent studies showed that multiple immunoreactive 4.1R proteins, varying in size from 30 to 210 kDa, are present in nucleated cells [ 4 , 5 ]. (portlandpress.com)
  • Many of the IEGs encode transcription factors whose expression is required for the activation of a class of delayed-response genes, which encode proteins that may contribute to the differentiation of NGF-treated PC12 cells ( 31 , 43 ). (asm.org)
  • Examples of such experiments include: Effects of a drug on sodium channels Inducible RNA interference system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein A more specific use of HEK 293 cells is in the propagation of adenoviral vectors. (wikipedia.org)
  • Cells transfected with vector containing the cDNA for the Na+/glucose cotransporter expressed Na(+)-dependent MeGlc transport. (diva-portal.org)
  • Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. (diva-portal.org)
  • Trypanosoma brucei cDNA libraries constructed in the vector pCDM8 were screened selectively for insect (procyclic) stage surface antigen cDNAs by transient expression in mammalian COS-7 fibroblasts and "panning" with a rabbit polyclonal antiserum. (ox.ac.uk)
  • Transfecting COS-1 cells with opossum kidney cDNA fractions resulted in similar to 70% inhibition of hypoxanthine influx suggesting the possible presence of genes within the library that act as apparent inhibitors of hypoxanthine transport. (kent.ac.uk)
  • Homogenates from COS cells transfected with the cDNA insert in the sense (S) but not the antisense (A) of the Thy-1 cDNA reacted with the 7C8 antibody. (nih.gov)
  • Transient expression of the mutant LCAT Fin cDNA in COS cells disclosed markedly diminished LCAT enzyme activity. (ahajournals.org)
  • The RhAG cDNA was subcloned into a retroviral vector, and a stable COS-1 cell line was then established via retroviral transduction. (elsevier.com)
  • Using the same strategy, however, we could not express RhD epitopes on COS-1 cells even in the presence of RhAG cDNA, suggesting that other factors might be required for the surface expression of RhD antigen. (elsevier.com)
  • Screening of cDNA libraries from the leukemia Jurkat cell line and from the adult human thymus resulted in the isolation of a novel protein of 793 amino acids (89,758 Da). (aacrjournals.org)
  • In this paper we describe the function of SLC19A3, another member of this transporter family most recently cloned, after transient transfection of the cDNA into HeLa cells. (chemweb.com)
  • In endothelial cells and cells transfected with the eNOS cDNA, eNOS behaves as a peripheral membrane protein that is regulated by the allosteric activator, calmodulin (CaM). (ahajournals.org)
  • LPL was expressed in CHO cells transfected with human LPL cDNA. (nii.ac.jp)
  • Expressed protein extraction from cos cells? (bio.net)
  • Spectrally resolved imaging of Green fluorescent protein (GFP) expressed in living COS-7 kidney cells distinguished the subcellular localization and demarcated the processes of protein folding and chromophore formation. (spie.org)
  • Protein kinase C (PKC) regulation of l -ascorbic acid transport mediated by the Na + /ascorbic acid transporters, hSVCT1 and hSVCT2, expressed in COS-1 cells was studied using recombinant carboxyl-terminal V5 epitope-tagged forms of the transporters. (physiology.org)
  • Fluorescent antibody staining of PSSA-1 transfected COS cells with a panel of procyclin-specific monoclonal antibodies confirmed that the protein was located on the outer surface of the plasma membrane. (ox.ac.uk)
  • We have used 3 main methods to disrupt endocytosis in cells: use of inhibitory peptides, overexpression of protein domains and RNAi. (endocytosis.org)
  • Thy-1, a glycosylphosphatidylinositol-linked integral membrane protein of the immunoglobulin superfamily, is a component of both large dense-core and small clear vesicles in PC12 cells. (nih.gov)
  • Clone 112 expressed a protein that comigrated with the 7C8 antigen expressed by PC12 cells, but untransfected COS cells (−) and cells transfected with plasmid 11 did not. (nih.gov)
  • A truncation mutant of TIA-1 (TIA-1ΔRRM), which acts as a transdominant inhibitor of SG assembly, promotes the expression of cotransfected reporter genes in COS transfectants, suggesting that this process of mRNA triage might, directly or indirectly, influence protein expression. (rupress.org)
  • Extending across a membrane, usually referring to a protein subunit that is exposed on both sides of a cell membrane. (ndif.org)
  • In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. (ijbiotech.com)
  • In conclusion, by using a Lentivirus-based gene delivery system, large amounts of recombinant human AAT protein were expressed in both CHO and COS-7 cell lines. (ijbiotech.com)
  • 2004). However, a major disadvantage of a prokaryotic expression system is that protein processing in bacteria occurs by a mechanism that differs significantly from that in mammalian cells (Gaillet et al. (ijbiotech.com)
  • In the Rh blood system, RhAG (Rh-associated glycoprotein, or Rh50) is thought to be involved in Rh30 (D, CE) expression by forming a protein complex on the red cell surface. (elsevier.com)
  • The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (avivasysbio.com)
  • To test if USP33-mediated accelerated deubiquitination could affect the stability of receptorC-arrestin complexes (5), we overexpressed USP33 in HEK-293 cells along with the V2R and -arrestin-2-GFP and examined the distribution of each protein by confocal microscopy. (biologycu.org)
  • The present study was conducted to determine if progesterone (P4) would inhibit oxytocin-stimulated phosphoinositide hydrolysis in COS-7 cells expressing transfected ovine oxytocin receptor (OTR) with little or no nuclear P4 receptor (nPR) protein present. (elsevier.com)
  • Radioreceptor assay for [ 3 H]-OT binding confirmed the presence of a high affinity binding site for OT in transfected cells, while treatment with P4 and GTPγS (which uncouples the OTR from the heterotrimeric G-protein) increased the K d for OT binding slightly. (elsevier.com)
  • We conclude that P4 can inhibit OTR-mediated phosphoinositide hydrolysis in COS-7 cells that express little or no nPR protein. (elsevier.com)
  • Radioreceptor assay for [3H]-OT binding confirmed the presence of a high affinity binding site for OT in transfected cells, while treatment with P4 and GTPγS (which uncouples the OTR from the heterotrimeric G-protein) increased the Kd for OT binding slightly. (elsevier.com)
  • Immunoblot analysis utilizing anti-casein kinase II antiserum of cytosolic extracts prepared from COS-1 cells co-transfected with pCMV-alpha and pCMV-beta showed 2- and 4-fold increases in immunoreactive alpha and beta subunit protein, respectively, relative to vector-transfected cells. (umassmed.edu)
  • COS-1 cells transfected with pCMV-alpha alone exhibited a 3-fold increase in immunoreactive alpha subunit protein and a nearly 2-fold increase in cytosolic casein kinase II catalytic activity. (umassmed.edu)
  • No increase in immunoreactive alpha subunit protein was observed in pCMV-beta-transfected cells, and no increase in immunoreactive beta subunit protein was observed in pCMV-alpha-transfected cells. (umassmed.edu)
  • However, CLIP-tag, SNAP-tag and cell surface-specific ACP/MCP-tag systems can specifically label subpopulations of target protein expressed on the cell surface using non-cell permeable substrates (3). (neb.com)
  • This approach permits discrimination of different populations of a cell surface protein: those properly translocated to the plasma membrane from those retained in the secretory pathway or already internalized ( e.g. upon ligand binding). (neb.com)
  • Once the fluorophore is coupled to the desired protein, the label fluoresces permitting visualization in living or fixed cells. (neb.com)
  • For example, co-regulation of the peroxisomal membrane protein PEX11β with mitochondrial respiration factors led us to discover an organelle interface between peroxisomes and mitochondria in mammalian cells. (nature.com)
  • The purified Bad (wild-type)-DTTR protein showed cytotoxicity to human glioma cells in a dose-dependent manner. (aacrjournals.org)
  • The Bad (S112A S136A)-DTTR protein altered the subcellular distribution of Bcl-X L , indicating that it enters the cell cytoplasm and binds Bcl-X L . Bad (S112D S136A)-DTTR, mutated to mimic phosphorylation of Bad, showed lower toxicity than either Bad (wild-type)-DTTR or Bad (S112A S136A)-DTTR, additionally indicating that Bad-DTTR must bind Bcl-X L to stimulate apoptosis. (aacrjournals.org)
  • The fusion protein was effective at inducing apoptosis in human glioma cells and prostate cancer cells. (aacrjournals.org)
  • A key immune checkpoint is triggered when PD-1 (programmed cell death protein 1) engages its ligand PD-L1. (cellsignal.com)
  • To understand better the dynamics of intermediate filament formation, transport, and stability in a healthy, living cell , we inserted neurofilament heavy chain (NFH)-green fluorescent protein (GFP) fusion constructs in adenoviral vectors with tetracycline (tet)-regulated promoters. (nasa.gov)
  • Considerable amounts of LPL enzyme protein mass were detected in the culture medium of the COS-1 cells transfected with wild type LPL, LPL-S132N or LPL-S132G. (nii.ac.jp)
  • Combined immunocytochemical detection of sst5 and visualization of a fluorescent SRIF analog by confocal microscopy revealed that whereas the internalized ligand progressively clustered toward the cell center with time, immunoreactive receptors remained predominantly associated with the plasma membrane. (nih.gov)
  • Due to the net positive surface charge these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. (springer.com)
  • Macropinocytosis is a triggered process used by the cell to internalize large amounts of fluid and membrane. (springer.com)
  • Here we show that both in fibroblasts and COS cells, membrane localization of Tiam1 is required for the induction of membrane ruffling. (nih.gov)
  • For vitamin C to act inside cells, it must be first transported across the plasma membrane, and the transport of ascorbate into mammalian cells is mediated by Na + -dependent uptake systems ( 12 ). (physiology.org)
  • Membrane IgG H chains turn over considerably more rapidly than secretory Ig H chains in the 18-81 A2 pre-B cell line. (jimmunol.org)
  • Accelerated proteasomal degradation of membrane Ig H chains was also observed in transfected nonlymphoid cells. (jimmunol.org)
  • We have previously described a pre-B cell line in which the membrane form of the Ig H chain is rapidly degraded in a prelysosomal compartment, whereas the secretory form is relatively stable ( 35 ). (jimmunol.org)
  • The process by which a cell receives and acts on some external chemical or physical signal, such as a hormone, including receiving the information at specific receptors in the plasma membrane, conveying the signal across the plasma membrane into the cell, and subsequently inducing an intracellular chain of other signalling molecules, thereby stimulating a specific cellular response. (ndif.org)
  • These data support a role for a non-genomic action for P4 in OTR signaling via some mechanism other than by binding to a membrane progestin receptor in an immortalized, transfected cell. (elsevier.com)
  • African Green Monkey Kidney Fibroblasts with Fluorescein, Alexa Fluor 568, and DAPI - An adherent COS-7 culture was immunofluorescently labeled with primary anti-bovine alpha -tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein. (fsu.edu)
  • COS-7 Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and Hoechst 33258 - A culture of COS-7 fibroblasts was labeled for mitochondria with MitoTracker Red CMXRos, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, a cyclic peptide derived from the toxic death cap fungus ( Amanita phalloides ). (fsu.edu)
  • Northern analyses of total cellular RNA prepared from COS-1 fibroblasts 65 h after transfection with pCMV-alpha or pCMV-beta or with both expression constructs showed marked specific increases in corresponding alpha and beta subunit RNAs. (umassmed.edu)
  • To elucidate the mechanisms regulating cell surface availability of the somatostatin (SRIF) receptor subtype sst5, we characterized its internalization properties in transfected COS-7 cells using biochemical, confocal microscopic, and electron microscopic techniques. (nih.gov)
  • The preservation of cell surface receptors was confirmed by binding experiments on whole cells revealing a lack of saturability of [125I]Tyr0-D-Trp8-SRIF binding at 37 C. Binding was rendered saturable by the drug monensin, showing that receptor recycling played a key role in the preservation of cell surface receptors. (nih.gov)
  • The ability of extracts of winter savory, purple coneflower, buckwheat and black elder to dose-dependently activate peroxisome proliferator-activated receptor-γ was determined in a reporter gene assay in COS-1 cells. (biomedsearch.com)
  • Although dose-dependent peroxisome proliferator-activated receptor-γ activity could be shown in COS-1 cells, the experiments in db/db mice lacked to confirm any anti-diabetic effect of the plant extracts in vivo and emphasizes the importance of verifying cell culture data using an appropriate in vivo model. (biomedsearch.com)
  • Calnexin has been reported to bind unassembled B cell receptor complexes ( 14 , 15 , 32 , 33 ). (jimmunol.org)
  • The cell-surface RAGE [receptor for AGE (advanced glycation end-products)] is associated with the development of diabetic vascular complications, neurodegenerative disorders and inflammation. (portlandpress.com)
  • In this study, we investigated the phosphorylation and compartmentalization of PLD1 in terms of CCh signaling in M3 muscarinic receptor (M3R)-expressing COS-7 cells. (elsevier.com)
  • To determine whether extracellular Bad can be delivered into cells via cell surface receptor binding and induce apoptosis, we genetically fused the mouse Bad gene to the gene for the translocation and receptor-binding domains of diphtheria toxin (DTTR). (aacrjournals.org)
  • We investigated the molecular basis of desensitization and trafficking of the CysLT 1 receptor constitutively and transiently expressed in the human monocyte/macrophage-like U937 or COS-7 cells in response to LTD 4 or nucleotides. (biologists.org)
  • These signaling pathways were totally inhibited by different specific CysLT 1 -receptor antagonists, and no CysLT 2 receptor mRNA was detected, which indicates that in U937 cells LTD 4 -induced responses can be totally ascribed to a CysLT 1 receptor. (biologists.org)
  • Flibanserin-induced reduction in firing rate in the cortex seems to be mediated through stimulation of postsynaptic 5-HT1A receptors, whereas the reduction of the number of active cells seems to be mediated through dopamine D4 receptor stimulation. (scribd.com)
  • The effects of NGF on PC12 cells are mediated by the NGF receptor TrkA ( 40 , 41 ). (asm.org)
  • These kinases contain an NH 2 -terminal catalytic domain and a COOH-terminal pleckstrin homologous domain, and their heterologous expression augments receptor activation of PKB, which suggests they are the primary signal transducers that enable PtdIns(3,4,5)P 3 or PtdIns- (3,4)P 2 to activate PKB and hence to control signaling pathways regulating cell survival, glucose uptake, and glycogen metabolism. (sciencemag.org)
  • The culture of transformed African green monkey kidney cells featured in the digital image above was labeled with Alexa Fluor 350 conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues, which are found in both mucoproteins and glycoproteins. (fsu.edu)
  • Golgi Apparatus in African Green Monkey Kidney Cells - In this section, a culture of transformed African green monkey kidney (COS-7 line) cells is presented that was labeled with Alexa Fluor 488 conjugated to the lectin wheat germ agglutinin. (fsu.edu)
  • COS-7 kidney cells were transfected by a plasmid pEGFP-N1 plasmid followed by incubation for 15 hours for gen expression. (spie.org)
  • The COS cell line was obtained by immortalizing a cell line derived from kidney cells of the African green monkey with a version of the SV40 genome that can produce large T antigen but has a defect in genomic replication. (signagen.com)
  • COS-7 monkey kidney cells (ATCC cat #CRL-1651) were grown in DMEM supplemented with 10% FCS. (moleculardevices.com)
  • Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. (wikipedia.org)
  • HEK 293 cells were generated in 1973 by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
  • Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
  • As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
  • COS cells are obtained by immortalizing CV-1 cells with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. (wikipedia.org)
  • When an expression construct with an SV40 promoter is introduced into COS cells, the vector can be replicated substantially by the large T antigen. (wikipedia.org)
  • These COS cells are genetically modified to produce the T antigen from their own genome. (wikipedia.org)
  • This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. (thermofisher.com)
  • The line was derived from the CV-1 cell line (ATCC CCL-70) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. (thermofisher.com)
  • CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). (harvard.edu)
  • The COS-7 cell line was derived by Yakov Gluzman in the early 1980s from the previously established CV-1 African green monkey kidney line by transformation of the normal cells with an origin defective mutant of simian virus 40 ( SV40 ) that codes for the wild-type virus T-antigen. (fsu.edu)
  • Cloning of a novel surface antigen from the insect stages of Trypanosoma brucei by expression in COS cells. (ox.ac.uk)
  • Furthermore, the antigen on COS cells was insensitive to treatment with phosphatidylinositol-specific phospholipase C suggesting that the inositol of the glycosylinositol phospholipid-lipid anchor contained the same fatty acyl modification reported recently for the procyclin molecule in cultured procyclic trypanosomes. (ox.ac.uk)
  • In contrast the PSSA-2 antigen on COS cells was stained very weakly by whole parasite antisera. (ox.ac.uk)
  • Expression of the 7C8 antigen in COS cells. (nih.gov)
  • To determine which of these two plasmids encoded the 7C8 antigen, we transfected each plasmid separately into COS cells, and probed Western blots of the cell homogenates with the 7C8 antibody. (nih.gov)
  • This study is to identify the preferred vaccine dosage (of antigen and adjuvant) and schedule (one or two administrations) of the cell-derived H1N1sw monovalent vaccine in healthy adults b. (bioportfolio.com)
  • These consist of receptors on the surface of activated T cells and their corresponding ligands on the surface of antigen presenting cells. (cellsignal.com)
  • To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)
  • Tunisia: Politics: Medicine: Stem Cell Research: Science: Biology: Wisconsin Technolgy Network: Governor Jim Doyle's proposal to build a $375 million interdisciplinary research center on the UW-Madison campus is characterized as a response to California, where voters passed a $3 billion, 10-year initiative to fund human embryonic stem cell research. (peacecorpsonline.org)
  • Scientists' expanding knowledge and understanding of stem cells is transforming the way we research human biology and medicine. (moleculardevices.com)
  • HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
  • To investigate the relative importance of these sites, the enzyme was over-expressed in COS M9 cells, and Ser → Ala mutations were introduced singly, or in combinations, at nine known phosphorylation sites. (elsevier.com)
  • and (iii) sites 3a and/or 3b can be phosphorylated in COS cells by mechanisms that do not depend on phosphorylation of site 5. (elsevier.com)
  • Evidence is provided that MAP kinase (ERK1 and ERK2) influences the cells' motility machinery by phosphorylating and, thereby, enhancing myosin light chain kinase (MLCK) activity leading to phosphorylation of myosin light chains (MLC). (rupress.org)
  • In contrast, expression of mutationally active MAP kinase kinase causes activation of MAP kinase leading to phosphorylation of MLCK and MLC and enhanced cell migration. (rupress.org)
  • We show here that MAP kinase activation is required for haptotaxis cell migration on a collagen substrate based on its ability to directly phosphorylate MLCK leading to the phosphorylation of MLC. (rupress.org)
  • CCh treatment of COS-7 cells transiently co-expressing PLD1 and M3R stimulated PLD1 activity and induced direct phosphorylation of PLD1 by PKC. (elsevier.com)
  • The CCh-induced activation and phosphorylation of PLD1 was completely blocked upon pretreatment of the cells with PKC-specific inhibitors. (elsevier.com)
  • These results demonstrate that stimulation of endothelial cells with vascular endothelial growth factor recruits eNOS and Akt to an adjacent region on the same domain of hsp90, thereby facilitating eNOS phosphorylation and enzyme activation. (ahajournals.org)
  • Both pDMAEMA- and PEI-complexed DNA showed colocalization with fluorescently-labeled transferrin and cholera toxin after internalization by COS-7 cells, which indicates uptake via the clathrin- and caveolae-dependent pathways. (springer.com)
  • The mRNA expression levels of the AAT genes were determined using Revearse Transcriptase-Polymerase Chain Reaction (RT-PCR) and its secretion into the medium by both cell types was determined using ELISA. (ijbiotech.com)
  • This expression system possesses key properties that ensure its application in the delivery of therapeutic genes into mammalian cultured cells. (ijbiotech.com)
  • Activation of the Ras/ERK pathway in NGF-treated PC12 cells has been shown to lead to the induction of a class of genes termed immediate-early genes (IEGs) ( 61 , 64 ). (asm.org)
  • Functionally important binding sites for GATA-1 are found in the regulatory regions of essentially all erythroid-cell-specific genes ( 30 ). (asm.org)
  • Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as experimental tools. (wikipedia.org)
  • This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a cell. (wikipedia.org)
  • In order to propagate such viral vectors, a cell line that expresses the missing genes is required. (wikipedia.org)
  • Since HEK 293 cells express a number of adenoviral genes, they can be used to propagate adenoviral vectors in which these genes (typically, E1 and E3) are deleted, such as AdEasy. (wikipedia.org)
  • This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. (avivasysbio.com)
  • This event is cell- and tissue-specific and dependent on the growth and differentiation stages of the cell [ 24 - 30 ]. (portlandpress.com)
  • The transcription factor GATA-1 is a key regulator of erythroid-cell differentiation and survival. (asm.org)
  • Since certain functions of GATA-1 are revealed only in an erythroid environment, GATA-1 constructs were examined for their ability to trigger terminal differentiation when introduced into a GATA-1-deficient erythroid cell line. (asm.org)
  • Intracellular dynamics of sst5 receptors in transfected COS-7 cells: maintenance of cell surface receptors during ligand-induced endocytosis. (nih.gov)
  • Mouse CD1d has also been found to control the function of a subset of T lymphocytes expressing receptors for NK cells (NKT cells) in a ligand-independent fashion that could be, however, augmented by the presence of glycosylceramides ( 8 , 9 , 10 ). (jimmunol.org)
  • All isolated cDNAs encode functional receptors because their transient expression in COS-7 cells resulted in a ligand-dependent increase in inositol phosphate production. (mendeley.com)
  • Since these mediators accumulate at sites of inflammation, and inflammatory cells express both classes of receptors, their responses are likely to be crossregulated. (biologists.org)
  • However, flibanserin displays higher affinity for 5-HT1A and 5-HT2A receptors in cloned cells than in cerebral tissue (the affinity for D4 receptors was not tested in tissues). (scribd.com)
  • The USDA and the FDA will host a joint meeting on October 23-24, 2018 on cell-based meat - meat produced by culturing animal cells outside of an animal - to thrash out how the sector might be regulated and how such products should be described on food labels. (foodnavigator-usa.com)
  • Released: Sep 28, 2018, Soft Cell released Electronic album Northern Lights / Guilty ('Cos I Say You Are) [Remixes]. (photographerspoint.com)
  • Here we report on palmitoylation of bovine opsin in COS-1 cells following expression of the synthetic wild-type opsin gene and several of its cysteine mutants in the presence of [3H]palmitic acid. (pnas.org)
  • COS-7 cells are susceptible to SV40 ( tsA209 strain) at 40 degrees Celsius, and SV40 mutants with deletions in certain regions. (fsu.edu)
  • Expression of the most active mutants correlated with increased glycogen accumulation in the COS M9 cells. (elsevier.com)
  • Additionally, we expressed the three mutants in the COS-1 cells and compared their secretion rates and levels of AT activity with those of the wild-type (WT). (nih.gov)
  • For more uses of In-Cell Westerns Assays , visit our website. (licor.com)
  • For those of you that like to watch videos and listen to information, here is a great webinar on In-Cell Western Assays . (licor.com)
  • Watch an introductory webinar to In-Cell Western Assays. (licor.com)
  • Cytotoxicity was determined using the proliferation (growth activity) and the plating efficiency (colony forming ability) assays after 24 hour incubation of COS 1 cells with different concentrations of methanolic flower extract from G. asclepiadea. (nel.edu)
  • The ImageXpress® Pico system does more than imaging-it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays. (moleculardevices.com)
  • While plant-based meat and dairy companies formed a trade association (the Washington DC-based Plant Based Foods Association ​ ​) in 2016, ​cell-based meat companies have not had a single point of contact with whom regulators at the FDA and the USDA and lawmakers on Capitol Hill can interact, noted Good Food Institute director of policy Jessica Almy during a panel debate on Friday. (foodnavigator-usa.com)
  • Cell 167 , 553-565.e12 (2016). (nature.com)
  • P.8918 right column bottom paragraph: 'HeLa and COS-7 cells expressing RFP fused to a nuclear localization signal were trypsinized, suspended in phosphate-buffered saline, and observed under an inverted microscope. (harvard.edu)
  • P.8919 left column top paragraph: 'The nuclear and cytoplasmic volumes of HeLa cells were 0.22 and 0.94 pl, respectively. (harvard.edu)
  • Differential expression of TopoIIα according to the tumor tissue and to the cell type has been reported. (aacrjournals.org)
  • Beating heart surgery appears to decrease red blood cell haemolysis, and tissue damage during the operative procedures and thereby significantly decreases the risk of plasma iron overload associated with conventional bypass. (chemweb.com)
  • After presumably adapting to tissue culture, cells from this clone developed into the relatively stable HEK 293 line. (wikipedia.org)
  • A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
  • Na(+)-dependent MeGlc uptake into transfected cells was saturable (Km 150 microM), phlorizin-sensitive (Ki 11 microM), and inhibited by sugar analogs (D-glucose greater than MeGlc greater than D-galactose greater than 3-O-methyl-D-glucoside greater than D-allose much greater than L-glucose). (diva-portal.org)
  • Expression of hSVCT1 and hSVCT2 in X enopus laevis oocytes or HRPE or COS-1 cells resulted in uptake of ascorbate that was absolutely dependent on Na + ( 4 , 12 , 19 , 27 , 28 ). (physiology.org)
  • RFL-6 rat lung fibroblast cells (ATCC cat #CCL-192) were grown in Ham's F-12 media supplemented with 20% fetal bovine serum, glutamine (2 mM), penicillin (50 U/mL) and streptomycin (10 mg/mL). (moleculardevices.com)
  • Skurat, AV , Cao, Y & Roach, PJ 1993, ' Glucose control of rabbit skeletal muscle glycogenin expressed in COS cells ', Journal of Biological Chemistry , vol. 268, no. 20, pp. 14701-14707. (elsevier.com)
  • Distribution of the 11 tryptophan residues studied and patterns of DAT immunoreactivity in COS cells expressing wild-type and these Trp-to-Ala mutant DATs. (aspetjournals.org)
  • Plant Extracts of Winter Savory, Purple Coneflower, Buckwheat and Black Elder Activate PPAR-γ in COS-1 Cells but do not Lower Blood Glucose in Db/db Mice In vivo. (biomedsearch.com)
  • Mice deficient for GATA-1 succumb to fatal anemia due to an inability of erythroid precursor cells to survive and mature ( 14 , 43 , 44 ). (asm.org)
  • Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells. (diva-portal.org)
  • Hr expression lowered levels of p53, p53 mediated reporter gene activity, and lower levels of the pro-apoptoticBcl2 family member Bax in COS cells. (elsevier.com)
  • A) Homogenates of COS cells transfected with two of the pcDM8 expression plasmids isolated from immunoreactive CHO cell clones were stained on a Western blot with the 7C8 antibody. (nih.gov)
  • Under these conditions, we detected significant expression of RhAG on the cell surface, compared to stable COS-1 cells transduced with the vector alone. (elsevier.com)
  • The molecular mechanisms involved in the variations in TopoIIα expression depend on the cell type. (aacrjournals.org)
  • The subcellular distribution of GFP-USP33 or HA-USP33 when immunostained alone (see and Fig. S3and = 3) of p-ERK in whole-cell extracts in HEK-293 cells with or without exogenous Mdm2 expression in response to isoproterenol stimulation for indicated times. (biologycu.org)
  • We used this inducible system for live cell imaging of changes in filament structure and cell shape, motility, and transport associated with increasing NFH-GFP expression. (nasa.gov)
  • Expression of DNM2-S619L in COS7 cells resulted in defective BIN1-dependent tubule formation. (biologists.org)
  • Although it has been known for some time that direct injection of 'naked' DNA allows transgene expression in muscle [ 2 ], in most of the cases 'naked' DNA molecules are not able to enter cells efficiently due to their large size, negative charge and nuclease mediated degradation in vivo [ 3 ]. (mdpi.com)
  • At different intervals the cells were examined by fluorescence microscopy and analyzed by a spectral imaging system. (spie.org)
  • We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic properties to that in the native brush border and to that expressed in Xenopus oocytes. (diva-portal.org)
  • He is a Rabbit aficionado (he has a dwarf rabbit named Peanut-who is the logo of Cos' Blog) and is a Golden Retriever connoisseur (he has two Goldens named Cassie and Chloe). (cosmelentertainment.com)
  • Flow cytometric analysis of untreated SUP-M2 cells using PDL1 (E1L3N ® ) XP ® Rabbit mAb #13864 (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red). (cellsignal.com)
  • This inhibition was specific to P4, because pre-treatment of cells with promegestone (R5020), testosterone, mifepristone (RU 486), or cortisol did not decrease OT-stimulated IP 3 levels. (elsevier.com)
  • Adult thymus, fetal thymus, fetal liver, and bone marrow, known as active tissues in terms of cell proliferation, are the tissues richest in ICBP90 mRNA. (aacrjournals.org)
  • In contrast, highly differentiated tissues and cells such as the central nervous system and peripheral leukocytes are free of ICBP90 mRNA. (aacrjournals.org)
  • In this In-Cell Western assay application, the response of COS-7 cells to increasing doses of hydroxyurea was measured by a specific antibody ( Anti-phospho-p53 from Cell Signaling Technology, P/N 9286 ) that detects phosphorylated-p53 (Ser16). (licor.com)
  • Members of this family consist of two heavy chains (200 kD) and two sets of light chains (16-20 kD) and are widely distributed in eukaryotic cells. (rupress.org)
  • Two forms of COS cell lines commonly used are COS-1 and COS-7. (wikipedia.org)
  • The COS cell lines are often used by biologists when studying the monkey virus SV40. (wikipedia.org)
  • Along those lines, can anyone explain the difference between COS-1 and COS-7 cells? (bio.net)
  • Shares of companies developing stem cell therapies surged Wednesday on news that the federal government has cleared 13 new stem cell lines for testing, bringing to a close nearly a decade of restrictions. (gatech.edu)
  • The transgene AAT-Jred chimer was transferred to CHO and COS-7 cell lines using this vector and its expressions were visualized by fluorescent microscopy. (ijbiotech.com)
  • Packaging cell lines are not required. (selectscience.net)
  • Find tissues and cell lines supported by DNA array analysis to express LEF1 . (avivasysbio.com)
  • Three CHO dhfr -stable cell lines were transfected with a supervector DNA specifying the murine V H and V L and human C H and C L . The resulting c11-1F4 mAbs produced by these mammalian cells were harvested, purified, and tested for their capacity to interact with amyloid in several in vitro and in vivo experimental systems. (aacrjournals.org)
  • Knockdown cell lines are particularly useful in gene function analysis, target discovery, target validation, assay development and compound screening. (sigmaaldrich.com)
  • Generating these knockdown cell lines is most readily accomplished by using lentivirus harboring an shRNA of interest. (sigmaaldrich.com)
  • The customized cell lines, produced by Sirion, are based on Sigma's MISSION ® shRNA libraries, a highly functionally validated RNAi library developed by The RNAi Consortium (TRC). (sigmaaldrich.com)
  • Sirion then continues with an optimized transduction of the specific shRNA lentivirus clones, generating stable, knockdown cell lines after propagating under puromycin selection. (sigmaaldrich.com)
  • The cell lines, along with a comprehensive data sheet explaining the knockdown analysis and propagation instructions are sent directly to the customer to begin using immediately. (sigmaaldrich.com)
  • Molecular analysis of the putative tumour-suppressor gene EXTL1 in neuroblastoma patients and cell lines. (cancerindex.org)
  • Recently, the EXTL1 gene was suggested as a candidate neuroblastoma-suppressor gene and to evaluate this hypothesis, we performed 1p deletion analysis and mutation screening of the EXTL1-coding region on DNA from 22 primary neuroblastomas and 21 neuroblastoma cell lines. (cancerindex.org)
  • Deletions of the chromosome region 1p36.1, including the EXTL1 gene, were detected in several neuroblastoma cell lines and primary tumours. (cancerindex.org)