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  • cDNA
  • I have recently stably-transfected COS-7 cells with cDNA cloned in the eukaryotic expression vector pREP9 (Invitrogen) which confers resistance to G418/geneticin and is maintained in cells as an episome (since it contains the origin of replication for EBV 'OriP' and also expresses the EBNA-1 gene necessary for vector replication via OriP). (bio.net)
  • However, I have been trying to transfect the 'pREP9-transfected' COS-7 cells with another cDNA cloned in another plasmid which confers resistance to hygromycin. (bio.net)
  • Trypanosoma brucei cDNA libraries constructed in the vector pCDM8 were screened selectively for insect (procyclic) stage surface antigen cDNAs by transient expression in mammalian COS-7 fibroblasts and "panning" with a rabbit polyclonal antiserum. (ox.ac.uk)
  • Cells transfected with vector containing the cDNA for the Na+/glucose cotransporter expressed Na(+)-dependent MeGlc transport. (diva-portal.org)
  • Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. (diva-portal.org)
  • Homogenates from COS cells transfected with the cDNA insert in the sense (S) but not the antisense (A) of the Thy-1 cDNA reacted with the 7C8 antibody. (nih.gov)
  • uptake
  • Na(+)-dependent MeGlc uptake into transfected cells was saturable (Km 150 microM), phlorizin-sensitive (Ki 11 microM), and inhibited by sugar analogs (D-glucose greater than MeGlc greater than D-galactose greater than 3-O-methyl-D-glucoside greater than D-allose much greater than L-glucose). (diva-portal.org)
  • The effects of several inhibitors of particular cellular uptake routes on the uptake of polyplexes and subsequent gene expression in COS-7 cells were investigated using FACS and transfection. (springer.com)
  • Both pDMAEMA- and PEI-complexed DNA showed colocalization with fluorescently-labeled transferrin and cholera toxin after internalization by COS-7 cells, which indicates uptake via the clathrin- and caveolae-dependent pathways. (springer.com)
  • antibody
  • In this In-Cell Western assay application, the response of COS-7 cells to increasing doses of hydroxyurea was measured by a specific antibody ( Anti-phospho-p53 from Cell Signaling Technology, P/N 9286 ) that detects phosphorylated-p53 (Ser16). (licor.com)
  • A) Homogenates of COS cells transfected with two of the pcDM8 expression plasmids isolated from immunoreactive CHO cell clones were stained on a Western blot with the 7C8 antibody. (nih.gov)
  • To determine which of these two plasmids encoded the 7C8 antigen, we transfected each plasmid separately into COS cells, and probed Western blots of the cell homogenates with the 7C8 antibody. (nih.gov)
  • plasmids
  • Another possibility may be that the cells were not able to support the episomal maintainance of two different plasmids with the same origin of replication? (bio.net)
  • intestinal
  • We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic properties to that in the native brush border and to that expressed in Xenopus oocytes. (diva-portal.org)
  • inhibition
  • In addition, activation of GABA receptors lead to the so-called shunting inhibition, which reduces the excitability of the cell independent of the changes in membrane potential. (wikipedia.org)
  • plasmid
  • One reason I thought of might be that the hygromycin resistance gene of my plasmid may not be expressed in these cells. (bio.net)
  • In terms of the cells, they were extremely easy to transfect with the initial pREP9 (20-30% efficiency of tranfection using a B-gal reporter plasmid) and the actual pREP9-transfectants are extremely easy to transiently transfect too (again, 20-30% efficiency of tranfection using a B-gal reporter plasmid). (bio.net)
  • membrane potential
  • When the reversal potential of chloride is less than the membrane potential, chloride ions flow down their electrochemical gradient into the cell. (wikipedia.org)
  • This influx of chloride ions lowers the membrane potential of the neuron, thus hyperpolarizes it, making it more difficult for these cells to conduct electrical impulses in the form of an action potential. (wikipedia.org)
  • glucose
  • Plant Extracts of Winter Savory, Purple Coneflower, Buckwheat and Black Elder Activate PPAR-γ in COS-1 Cells but do not Lower Blood Glucose in Db/db Mice In vivo. (biomedsearch.com)
  • It is the way all the animal cells have to store glucose. (wikipedia.org)
  • decreases
  • In human cells, tethering of hCUGBP1 to a mRNA decreases its steadystate suggesting the destabilization of the mRNA. (wikipedia.org)
  • fluorescence
  • At different intervals the cells were examined by fluorescence microscopy and analyzed by a spectral imaging system. (spie.org)
  • interactions
  • Due to the net positive surface charge these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. (springer.com)
  • suitable
  • In contrast, the MitoFluor probes are designed specifically for actively respiring cells and are not suitable for fixation and counterstaining procedures. (fsu.edu)
  • Primary
  • The single cell featured in the digital image above was resident in a COS-7 culture immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein isothiocyanate ( FITC ), which has an absorption maximum of 495 nanometers. (fsu.edu)
  • Culture
  • The filamentous actin and cell nuclei present in the culture, which were respectively labeled with Alexa Fluor 488 conjugated to phalloidin and Hoechst 33258, are also readily apparent. (fsu.edu)
  • The culture was counterstained for DNA in the cell nucleus with the ultraviolet-absorbing probe DAPI. (fsu.edu)
  • cellular
  • The zoning process for constructing new cellular telecommunications towers is very controversial, and often times, cell tower companies find themselves fighting against the municipality and local residents to obtain zoning approvals. (foxrothschild.com)
  • Western
  • The image represents a 96-well two-color In-Cell Western with the 700 and 800 nm channels detecting phosphorylated-p53 (Ser16) and total ERK1, respectively. (licor.com)