• Amyloidogenic hIAPP-transfected COS cells contained up to threefold more apoptotic cells present at 96 hours after transfection compared with the nonamyloidogenic vector controls. (elsevierpure.com)
  • To ascertain which receptor is accountable for the impact of CRTNF on DRG neurons, DRG neurons two days soon after siRNA transfection have been co-cultured with COS-7 cells expressing ether control GFP or CRTNF for 24 hrs. (atminhibitor.com)
  • Wild type huntingtin did not significantly modulate cell death caused by transfection of HD exon 1 fragments containing 21 CAG repeats in either cell line. (bmj.com)
  • Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. (qiagen.com)
  • Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. (qiagen.com)
  • cell type-specific transfection protocols are available. (qiagen.com)
  • While mesenchymal and epithelial cells are dominated by adhesive interactions the amoeboid crawling of leukocytes is largely controlled by guidance cues of the chemokine family 3 , 4 . (nature.com)
  • Golachowska, M. R., Hoekstra, D. & van IJzendoorn, S. C. Recycling endosomes in apical plasma membrane domain formation and epithelial cell polarity. (nature.com)
  • Hiddinga, HJ & Eberhardt, NL 1999, ' Intracellular amyloidogenesis by human islet amyloid polypeptide induces apoptosis in COS-1 cells ', American Journal of Pathology , vol. 154, no. 4, pp. 1077-1088. (elsevierpure.com)
  • Many surface membrane proteins are proteolytically released into a cell's secretome, where they can have additional functions in cell-cell-communication. (nih.gov)
  • Here, we set up and evaluated the 'surface-spanning protein enrichment with click sugars' (SUSPECS) method for cell surface membrane glycoprotein biotinylation, enrichment and label-free quantitative mass spectrometry. (nih.gov)
  • This approach permits discrimination of different populations of a cell surface protein: those properly translocated to the plasma membrane from those retained in the secretory pathway or already internalized ( e.g. upon ligand binding). (neb.com)
  • This gene subfamily is constitutively active at physiological resting membrane potentials in excitable cells, including smooth muscle cells, and has been particularly linked to the human pulmonary circulation. (ersjournals.com)
  • They span the membrane of cells, allowing the selective permeation of K + ions from one side of the membrane to the other, usually from the inside of the cell to the outside. (ersjournals.com)
  • They regulate the excitability of cells and contribute to their resting membrane potential [ 1 , 2 ]. (ersjournals.com)
  • Cells can be grown on the membrane just as they would on certain polymer film surfaces, such as Aclar®, polycarbonate, etc. (2spi.com)
  • And once the cells are processed (e.g. fixed) and washed, another cell can be glued to the first one creating a chamber that has the volume of two membrane window grids. (2spi.com)
  • All results shown on this webpage were from Cos 7 cells grown onto 100 nm thick silicon nitride membrane window grids, specifically, SPI #4122SN-BA. (2spi.com)
  • Twenty micro litres of dextran loaded phosphate buffer were then added and the silicon nitride membrane window cell sealed. (2spi.com)
  • Therefore we offer the Wet Cell Kits in a selection of different membrane thickness and we leave it to our customers to decide which is best for them. (2spi.com)
  • We also offer a "sampler" kit that includes 2 each of four different membrane thicknesses (e.g. 100 nm, 50 nm, 30 nm and 20 nm) plus 8 "blanks" so that there would be enough material to make a total of 8 different Wet Cells. (2spi.com)
  • C - F ) Coimmunoprecipitation assays with COS cell lysates. (jci.org)
  • WB: Transfected NIH/3T3 and COS-7 cell lysates. (abcam.com)
  • 汪艳 , 刘春新 , 易有荣 , 崔冶建 , 朱珺 , 董长垣 , 2006: Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods, Virologica Sinica, 21, 253-256. (virosin.org)
  • 吴海祥 , 郑从义 , 屈三甫 , 郭荆哲 , 王燕丽 , 2001: Studies on Apoptosis of Host Cells Induced by Foot-and-M outh Disease Virus, Virologica Sinica, 16, 175-178. (virosin.org)
  • 高 娟 , 杨守京* , 刘彦仿 , 2003: Preliminary Study of Apoptosis of SP2/0 Cells Induced by Hantaan virus, Virologica Sinica, 18, 315-317. (virosin.org)
  • To establish the mechanism of cell death, we transfected COS-1 cells with vectors expressing amyloidogenic hIAPP or nonamyloidogenic rat IAPP and mutant hIAPP constructs and assayed them for markers characteristic of apoptosis and necrosis by fluorescence-activated cell sorting analysis. (elsevierpure.com)
  • Ibuprofen and apigenin induce apoptosis and cell cycle arrest in activated microglia. (uni-bielefeld.de)
  • Potential antioxidant activity, cytotoxic and apoptosis-inducing effects of Chelidonium majus L. extract on leukemia cells. (nel.edu)
  • Kok LP. Boon's disease: hemorrhagic cystitis in conjunction with massive exfoliation of degenerated urothelial cells (apoptosis? (medscape.com)
  • Cells from these lines are also often transfected to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. (wikipedia.org)
  • Immunofluorescence-based confocal microscopy demonstrated that SUSPECS selectively labeled cell surface proteins. (nih.gov)
  • SNAP-tag, CLIP-tag™ and cell surface-specific ACP/MCP-tag systems can specifically label subpopulations of target proteins expressed on the cell surface using non-cell permeable substrates (3). (neb.com)
  • A ) Chimeric HDAC4/5 proteins (as indicated) were expressed in COS cells in the presence of CaMKIIδB-T287D. (jci.org)
  • COS are fibroblast-like cell lines derived from monkey kidney tissue. (wikipedia.org)
  • Cell migration is mainly governed by adhesion of cells to substrates (other cells or connective tissue) and by extracellular signalling molecules acting as motogenic stimuli or directional guidance cues 2 . (nature.com)
  • Tissue, cells or virus corresponding to V5 tag. (abcam.com)
  • Furthermore, we developed a novel multicolour light-sheet fluorescence microscopy (LSFM) approach for deciphering immune and infection processes in large tissue specimens on a single-cell level in 3 dimensions [7]. (uni-wuerzburg.de)
  • Yet, if tissue clearing is necessary, fixation of the tissues has limited real-time life cell imaging deep within tissues. (uni-wuerzburg.de)
  • Human islet amyloid polypeptide (hIAPP) is co-secreted with insulin from pancreatic islet β cells. (elsevierpure.com)
  • Our results directly illustrate the importance of GDH in the regulation of insulin secretion from pancreatic β-cells. (diabetesjournals.org)
  • Pancreatic islet cells. (guidetopharmacology.org)
  • COS cells are obtained by immortalizing CV-1 cells with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. (wikipedia.org)
  • When an expression construct with an SV40 origin of replication is introduced into COS cells, the vector can be replicated substantially by the large T antigen. (wikipedia.org)
  • These COS cells are genetically modified to produce the T antigen from their own genome. (wikipedia.org)
  • 2 The polyglutamine expansion mutation causes disease by conferring a novel deleterious function on the mutant protein and the severity correlates with increasing CAG repeat number and expression levels in transgenic mice and in cell culture models. (bmj.com)
  • METHODS We cotransfected neuronal (SK-N-SH, human neuroblastoma) and non-neuronal (COS-7, monkey kidney) cell lines with HD exon 1 (containing either 21 or 72 CAG repeats) construct DNA and either full length wild type huntingtin or pFLAG (control vector). (bmj.com)
  • Interestingly, protein changes at the cell surface only partly correlated with changes in the secretome. (nih.gov)
  • We initial confirmed that siRNA certain to TNFR1 or TNFR2 silenced the expression of TNFR1 and TNFR2 proficiently as evidenced by significantly reduce protein levels of TNFR1 ( 70 four knockdown) and TNFR2 ( 75 four.5 knock-down) observed in DRG neurons getting target specific siRNA compared with those observed in cells treated with manage siRNA (Fig. 3A). (atminhibitor.com)
  • One day later the cells have been harvested for determination in the NaV1.7, NaV1.eight, CaV3.two and CCL2 mRNA, NaV1.7, NaV1.8, CaV3.two protein levels and CCL2 release. (atminhibitor.com)
  • The impact of co-culture with CRTNFexpressing COS-7 cells around the expression of voltage gated cation channels and CCL2 mRNA (Fig. 4A), the protein levels of NaV1.7, NaV1.eight, CaV3.2 (Fig. 4B) in DRG neurons were not drastically G protein-coupled Bile Acid Receptor 1 supplier affected by the presence. (atminhibitor.com)
  • Once the fluorophore is coupled to the desired protein, the label fluorescesces permitting visualization in living or fixed cells. (neb.com)
  • Besides new methods for the study of conformational dynamics of biomolecules and protein folding, direct stochastic optical reconstruction microscopy ( d STORM) has been developed that enables super-resolution fluorescence imaging with standard organic fluorophores with a spatial resolution of ≤ 20 nm in the imaging plane in fixed and in living cells (Figure) [8]. (uni-wuerzburg.de)
  • We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • This substitution caused expression of a more unstable gene product, as evident from comparison of the relative levels of CYP2D6 mRNA, CYP2D6 protein, and bufuralol 1'-hydroxylase activities in pCMV2-CYP2D6-transfected COS-1 cells. (aspetjournals.org)
  • J Cell Biochem 72:94-102. (cdc.gov)
  • We demonstrated that COS-1 cells transfected with vectors expressing hIAPP exhibited intracellular amyloid deposits that were associated with cell death (O'Brien, Butler, Kreutter, Kane, Eberhardt, Am J Pathol 1995, 147:609-616). (elsevierpure.com)
  • Furthermore, several chimeric constructs of the CYP2D6wt and CYP2D6Ch genes were made, inserted into pCMV2 vectors, and expressed in COS-1 cells. (aspetjournals.org)
  • It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application. (bdbiosciences.com)
  • ACE2 is a SARS-CoV receptor located on the outer surface of cells.Applications:For. (topsan.org)
  • Agonist-stimulated calcium efflux in X. laevis oocytes or inositol phosphate accumulation in COS-7 cells was used to assess receptor activation. (nih.gov)
  • Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue. (nature.com)
  • Once in the lymph node, the cells experience a second chemokine, (C-C motif) ligand19 (CCL19), which interacts with the same receptor (C-C chemokine receptor 7, CCR7) but interacts only weakly with sugars. (nature.com)
  • Furthermore, both robust phosphorylation of Homer3 and its dissociation from metabotropic glutamate receptor 1α (mGluR1α) were triggered by depolarization in primary cultured Purkinje cells, and these events were inhibited by CaMKII inhibitor. (jneurosci.org)
  • While TR4 nuclear receptor plays key roles to promote prostate cancer progression, its roles to alter the progression of clear cell renal cell carcinoma (ccRCC), remains unclear. (bvsalud.org)
  • The activity of the mutant GDH (GDH266C), expressed in COS-7 cells, was constitutively elevated, and allosteric regulations by ADP and GTP were severely impaired. (diabetesjournals.org)
  • COS cells) indicate replication of the virus. (cdc.gov)
  • A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. (bvsalud.org)
  • Endocytosis is a fundamental process involved in trafficking of various extracellular and transmembrane molecules from the cell surface to its interior. (mdpi.com)
  • Ith CRTNF-expressing COS-7 cells, there was no modify inside the mRNA expression of NaV1.7, NaV1.8, or CaV3.two in DRG neurons exposed to sTNF (Fig. 2A). (atminhibitor.com)
  • We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. (nature.com)
  • The prevailing paradigm of chemokine function is that spatial diffusion-based gradients of chemokines induce polarization and directed migration of the responding cells towards the chemokine source 5 . (nature.com)
  • The best understood example for the significance of immobilized vs. soluble chemokine gradients is the trafficking of dendritic cells (DCs). (nature.com)
  • These experiments provide direct evidence that intracellular hIAPP amyloid causes cell death by triggering apoptotic pathways. (elsevierpure.com)
  • Continuous interleukin-6 application in vivo via macroencapsulation of interleukin-6-expressing COS-7 cells induces massive gliosis. (uni-bielefeld.de)
  • Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. (nature.com)
  • Preclinical studies using the in vivo mouse model with xenografted RCC Caki-1 cells into the sub-renal capsule of nude mice also found that TR4 could promote the ccRCC VM and its associated metastasis via modulating the miR490-3p/VIM signals. (bvsalud.org)
  • To address these complex challenges of a dysfunctional immune system caught between defective tolerance and compromised immunity we employ optical imaging methods, i.e. optical techniques that provide single cell resolution in the context of intact tissues. (uni-wuerzburg.de)
  • Phorbol esters (PE) are analogues of DAG and potent tumour promoters that cause a variety of physiological changes when administered to both cells and tissues. (embl-heidelberg.de)
  • 24] (Santa Cruz Biotechnologies) or car (DMSO) and just after 4 hrs of inhibitor treatment cocultured with COS-7 cells expressing GFP or CRTNF. (atminhibitor.com)
  • Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized COS-7 using RNeasy Mini Kit from Qiagen. (labettor.com)
  • However, Rigamonti et al 6 recently showed that wild type huntingtin can protect CNS cells from a variety of apoptotic stimuli, including serum withdrawal, stimulation of death receptors, and pro-apoptotic Bcl-2 homologues. (bmj.com)
  • For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s). (bdbiosciences.com)
  • The Beilhack group has been investigating immune cell interactions after allogeneic hematopoietic cell transplantation (HCT). (uni-wuerzburg.de)
  • CONCLUSIONS Our results suggest that wild type huntingtin can significantly reduce the cellular toxicity of mutant HD exon 1 fragments in both neuronal and non-neuronal cell lines. (bmj.com)
  • This enables cells to communicate and respond to external environments, maintain cellular homeostasis, and transduce signals. (mdpi.com)
  • It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. (nature.com)
  • Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. (nature.com)
  • Together, these findings suggested that Homer3 in Purkinje cells might function as a reversible coupler regulated by CaMKII phosphorylation and that the phosphorylation is capable of regulating the postsynaptic molecular architecture in response to synaptic activity. (jneurosci.org)
  • These results suggest that the phosphorylation may regulate postsynaptic molecular architecture and Ca 2+ signaling of Purkinje cells in response to synaptic activity. (jneurosci.org)
  • 1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
  • There is probably no single one chemical exposure responsible for these illnesses, but more likely a mixed exposure of chemical classes such as particulate matter and oxygenated organic species (oxidized volatile organic compounds that are likely biologically reactive and potentially cause cell damage and increase susceptibility to disease). (cdc.gov)
  • 王平忠 , 白雪帆 , 潘 蕾 , 张颖 , 黄长形 , 2003: Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome, Virologica Sinica, 18, 420-422. (virosin.org)
  • 刘超红 , 焦成松 , 陈新文 , 2006: Cloning of HCV ns2 Gene and its Expression in Prokaryotic and Mammalian cells, Virologica Sinica, 21, 217-220. (virosin.org)
  • These findings reveal a central role for HDAC4 in CaMKII signaling pathways and have implications for the control of gene expression by calcium signaling in a variety of cell types. (jci.org)
  • Then the expression will be cos(Mat(1)) . (originlab.com)
  • To evaluate the origin of the detrimental mutation in the genes, parts of the 5' flanking regions were introduced into a Hep G2/simian virus 40 expression system with chloramphenicol acetyltransferase as a reporter gene, and transfected cells were analyzed for activity. (aspetjournals.org)
  • Bexarotene suppressed AtT-20 cell growth, proopiomelanocortin (POMC) expression and adrenocorticotropin (ACTH) secretion. (bvsalud.org)
  • The cells were grown overnight at 37°C (98.6° F) in an incubator in a chamber consisting of an internally threaded aluminum cylinder epoxied to the membranes. (2spi.com)
  • I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. (labettor.com)
  • This peptide spontaneously aggregates in the form of fibrils, and amyloid deposits are associated with dead or degenerating β cells, a hallmark of noninsulin-dependent diabetes mellitus. (elsevierpure.com)
  • The effect of the unregulated increase in GDH activity on insulin secretion was examined by overexpressing GDH266C in an insulinoma cell line, MIN6. (diabetesjournals.org)
  • Three COS lines were created (COS-1, COS-3 and COS-7), of which two are commonly used (COS-1 and COS-7). (wikipedia.org)
  • The COS cell lines are often used by biologists when studying the monkey virus SV40. (wikipedia.org)
  • Cycle threshold (Ct) changes measured by real-time polymerase chain reaction versus days after infection of the indicated cell lines. (cdc.gov)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • RESULTS Full length wild type huntingtin significantly reduced cell death resulting from the mutant HD exon 1 fragments containing 72 CAG repeats in both cell lines. (bmj.com)
  • The aim of this study was to investigate the effect of miR-4778-3p on the radiosensitivity of cervical cancer cells and to elucidate the underlying mechanism. (bvsalud.org)
  • B ) An HDAC5 mutant, in which the CaMKII consensus sites were mutated to the corresponding sites in HDAC4, was expressed in COS cells alone or with CaMKI c.a. and CaMKIIδB-T287D. (jci.org)
  • C ) COS cells were cotransfected with FLAG-pcDNA, -HDAC4, or -HDAC5 and Myc-CaMKIIδB-T287D. (jci.org)
  • Bone marrow stromal cells reduce ischemia-induced astrocytic activation in vitro. (uni-bielefeld.de)
  • Although it is conceivable that cells can equally respond to gradients of soluble and/or immobilized chemokines, almost all cell biological information available about-gradient sensing is based on in vitro studies employing soluble gradients. (nature.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • We first demonstrate that the splitting of the lateral plate into the two cell sheets progresses in an anteroposterior order and this progression is not coordinated with that of the somitic segmentation. (biologists.com)
  • The cell surface proteome is dynamic and has fundamental roles in cell signaling. (nih.gov)
  • Yet, it remains challenging to determine the surface proteome and to compare it to the cell secretome, under serum-containing cell culture conditions. (nih.gov)
  • cell surface display technologies. (nih.gov)
  • GFP) cannot specifically visualize cell surface subpopulations. (neb.com)
  • It is not clear if this protective effect was confined to particular cell types, or if wild type huntingtin exerted its protective effect in this model by simply reducing the formation of toxic proteolytic fragments from mutant huntingtin. (bmj.com)
  • This suggests that wild type huntingtin can be protective in different cell types and that it can act against the toxicity caused by a mutant huntingtin fragment as well as against a full length transgene. (bmj.com)
  • Incubation of transfected COS-1 cell cytosols at 37 degrees showed that the time for decline of NAT activity to 50% of its initial value was 45 hr for NAT-1, 60 hr for NAT-2(99asn), and 4 hr for NAT-2(99ile). (cdc.gov)
  • T cells stably expressing SARS-CoV Spike Glycoprotein. (topsan.org)
  • Indeed we believe the SPI SEM Wet Cell Kit™ could be used quite successfully on any SEM, one that need not have LaB6 or FESEM capabilities. (2spi.com)
  • One unit of this adhesive, more than enough to glue ten cells, is included with each kit. (2spi.com)
  • The specific impact of these factors differs considerably between cell types. (nature.com)
  • C) Jurkat cells were labeled with an anti-ceramide C16 antibody and a secondary Alexa 647 labeled antibody. (uni-wuerzburg.de)
  • Note: When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed. (bdbiosciences.com)
  • T6796CHO-K1 cells were stably infected with Human ACE2. (topsan.org)
  • T6029293T cells stably expressing Human ACE2Applications:For Research Use OnlyStorage Condition:Liquid nitrogen vapour phase. (topsan.org)
  • However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. (bdbiosciences.com)