Cortical membrane potential voltage sensitive dye imaging. Medical search. Frequent questions

We carried out multi-site voltage-sensitive dye imaging of membrane potential transients from thin basal branches of prefrontal cortical pyramidal neurons before and after application of channel blockers. (yale.edu)
Channel distributions and all other biophysical properties of model basal dendrites of prefrontal layer V pyramidal cell from Acker, Antic (2008) Membrane Exitability and Action Potential Backpropagation in Basal Dendrites of Prefrontal Cortical Pyramidal Neurons. (yale.edu)
Strengths of imaging brain activity with voltage-sensitive dyes include the following abilities: Measurement of population signals from many areas may be taken simultaneously, and hundreds of neurons may be recorded from. (wikipedia.org)
We show that predicted cortical state is correlated between groups of 4 neurons, introducing the possibility of spike time pattern modulation by population-wide trial-to-trial changes in excitability (i.e. cortical state). (nature.com)
Sections focus on imaging at the molecular level, axons and nerve terminals, spines and dendrites, neurons and circuits in vitro, neurons and circuits in vivo, glia, brain dynamics and behavior, and brain pathology. (cshlpress.com)
Antic S, Major G, Zecevic D (1999) Fast optical recordings of membrane potential changes from dendrites of pyramidal neurons. (yale.edu)
Antic S, Zecevic D (1995) Optical signals from neurons with internally applied voltage-sensitive dyes. (yale.edu)
Colbert CM, Pan E (2002) Ion channel properties underlying axonal action potential initiation in pyramidal neurons. (yale.edu)
Connors BW, Prince DA (1982) Effects of local anesthetic QX-314 on the membrane properties of hippocampal pyramidal neurons. (yale.edu)
Grinvald A, Salzberg BM, Lev-Ram V, Hildesheim R (1987) Optical recording of synaptic potentials from processes of single neurons using intracellular potentiometric dyes. (yale.edu)
In this review, we evaluate the recent developments in imaging and electrophysiological techniques used to perform multiple single neurons recordings on microcircuits ex vivo . (frontiersin.org)
We studied the spatial organization of directionally selective neurons in the cortical middle temporal visual area (area MT) of the Cebus monkey. (jneurosci.org)
After training, mice could detect a photostimulus firing a single action potential in approximately 300 neurons. (nature.com)
Even fewer neurons (approximately 60) were required for longer stimuli (five action potentials, 250 ms). Our results show that perceptual decisions and learning can be driven by extremely brief epochs of cortical activity in a sparse subset of supragranular cortical pyramidal neurons. (nature.com)
In this report, using in vitro neuronal cultures, ex vivo organotypic brain slice cultures and acute brain slice preparations, we established that cerebellar granule (CbG) and hippocampal CA1 neurons were significantly more sensitive to OS (induced by paraquat) than cerebral cortical and hippocampal CA3 neurons. (biomedcentral.com)
Subsequent targeted biochemical analyses confirmed the lower energy levels (in the form of ATP) in primary CbG neurons compared with cortical neurons. (biomedcentral.com)
Since OS-sensitive neurons might be the ones that degenerate early during the aging process or in certain neurodegenerative diseases [ 1 ], study of the molecular mechanisms of SNV to OS may offer insights into both aging-associated and disease-initiated neurodegeneration, as well as provide leads to the protection of vulnerable neurons. (biomedcentral.com)
To study the relationship between SNV and OS, we thought it necessary to determine differences in the redox status and OS-handling capacity of both OS-sensitive and OS-resistant neurons. (biomedcentral.com)
In the following, we will apply this approach in order to investigate which of the two mechanisms is at work in primary motion-sensitive neurons of the fruit fly Drosophila . (elifesciences.org)
cause capacitive displacement currents that indirectly lead to slow membrane charge accumulation (on a time scale of tens of milliseconds), ultimately bringing the neurons to their AP discharge threshold. (eneuro.org)
Neurons in the brain communicate via action potentials, which are small and fast changes in the voltage of the cell membrane [ 1 ]. (hindawi.com)
Action potential is the unit of information processing in neurons, and as a result many neuroscience research projects involve recordings of action potentials or action potential sequences from single neurons or neural networks. (hindawi.com)

Hádinger N, Bősz E, Tóth B, Vantomme G, Lüthi A, Acsády L. Region-selective control of the thalamic reticular nucleus via cortical layer 5 pyramidal cells. (unil.ch)

Voltage-sensitive dyes, also known as potentiometric dyes, are dyes which change their spectral properties in response to voltage changes. (wikipedia.org)
Potentiometric dyes are used to monitor the electrical activity inside cell organelles where it is not possible to insert an electrode, such as the mitochondria and dendritic spine. (wikipedia.org)

Many physiological processes are accompanied by changes in cell membrane potential which can be detected with voltage sensitive dyes. (wikipedia.org)
One of the first electrochemical models proposed that the cell membrane exerts linear physicochemical changes ( 12 , 13 ) to assess the EMF parameters for which biological effects might be expected. (frontiersin.org)
During periods of inactivity, the cell membrane of a neuron is typically hyperpolarized to about −60 mV (cell's interior environment negative). (hindawi.com)

Here, using simultaneous voltage and calcium two-photon imaging of Purkinje neuron spiny dendrites, we show how coincident sub- and suprathreshold synaptic inputs modulate dendritic calcium signaling during sensory stimulation in awake mice. (oist.jp)
Sensory stimulation evokes subthreshold excitatory and inhibitory post-synaptic potentials, that coincide with suprathreshold dendritic spikes triggered by climbing fiber and parallel fiber synaptic input. (oist.jp)
Intrinsic supra-linear dendritic mechanisms, including voltage gated calcium channels and metabotropic glutamate receptors, are recruited cooperatively to expand the dynamic range of sensory evoked dendritic calcium signals. (oist.jp)
Membrane voltage oscillations in layer 1 of primary sensory cortices might be important indicators of cortical gain control, attentional focusing, and signal integration. (oist.jp)
Alonso BD, Lowe AS, Dear JP, Lee KC, Williams SC, Finnerty GT (2008) Sensory inputs from whisking movements modify cortical whisker maps visualized with functional magnetic resonance imaging. (springer.com)

In contrast, pharmacologically blocking the delayed rectifier potassium, voltage-gated calcium or Ih, conductance had little effect on dendritic action potential propagation. (yale.edu)
Dendritic coincidence detection is thought fundamental to neuronal processing, yet the basic dendritic voltage-calcium relationship remains unexplored in awake animals. (oist.jp)

The new voltage-sensitive dye di1-ANNINE-6plus allows to label layer 1 in vivo without removing the dura (a-e). (oist.jp)
A , Long-term in vivo imaging of GFP-expressing axons in the adult mouse barrel cortex. (jneurosci.org)
Here we combined computational simulations with analysis of in vivo 2-photon Ca 2+ imaging data from somatosensory cortex of Fmr1 knock-out (KO) mice, a model of Fragile-X Syndrome, to test the E/I imbalance theory. (biorxiv.org)
Debarbieux F, Audinat E, Charpak S (2003) Action potential propagation in dendrites of rat mitral cells in vivo. (yale.edu)

Multi-electrode arrays, calcium imaging, voltage-sensitive dyes and intrinsic imaging measure mesoscale population activity. (visionsciences.org)
In addition, several intrinsic and extrinsic noise sources are present during the recordings, affecting the signal-to-noise ratio of the measured voltages. (hindawi.com)

1 . Acker CD, Antic SD (2009) Quantitative assessment of the distributions of membrane conductances involved in action potential backpropagation along basal dendrites. (yale.edu)
Djurisic M, Antic S, Chen WR, Zecevic D (2004) Voltage imaging from dendrites of mitral cells: EPSP attenuation and spike trigger zones. (yale.edu)
Bischofberger J, Jonas P (1997) Action potential propagation into the presynaptic dendrites of rat mitral cells. (yale.edu)
Christie JM, Westbrook GL (2003) Regulation of backpropagating action potentials in mitral cell lateral dendrites by A-type potassium currents. (yale.edu)
With this system we could detect [Ca 2+ ] i and [Na + ] i changes from single action potentials in axons and synaptically evoked signals in dendrites, both with submicron resolution and a good signal-to-noise ratio (S/N). (eneuro.org)

How information in the nervous system is encoded by patterns of action potentials (i.e. spikes) remains an open question. (nature.com)
Barth DS, Di S, Baumgartner C (1989) Laminar cortical interactions during epileptic spikes studied with principal component analysis and physiological modeling. (springer.com)
Voltage spikes acquired in extracellular recording are typically between 50 μ V to 500 μ V peak-to peak, with rise times of 0.2 ms or more and pulse durations of 1 ms or more [ 8 ]. (hindawi.com)

Engelhardt JK, Morales FR, Chase MH (1998) An alternative method for the analysis of neuron passive electrical data which uses integrals of voltage transients. (yale.edu)

Chen WR, Shepherd GM (1997) Membrane and synaptic properties of mitral cells in slices of rat olfactory bulb. (yale.edu)

Lysosomes are membrane bound acidic intracellular organelles filled with hydrolytic enzymes that normally function as recycling centers within cells by breaking down damaged cellular macromolecules. (stanford.edu)
It can reveal information about the location of various calcium channels and calcium permeable receptors, the time course, magnitude, and location of intracellular calcium concentration ([Ca 2+ ] i ) changes, and indirectly, the occurrence of action potentials. (eneuro.org)

MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
Activated (GTP-bound) Ras is able to recruit Raf to the plasma membrane and activate it. (forex-consultant.com)

In this study, differentiating murine cortical networks on multiwell microelectrode arrays were repeatedly exposed to an extremely low-electromagnetic field (ELEMF) with alternating 10 and 16 Hz frequencies piggy backed onto a 150 MHz carrier frequency. (frontiersin.org)
We consider the available experimental techniques that allow a comprehensive assessment of circuit dynamics, including voltage and calcium imaging and extracellular electrophysiological recordings with multi-electrode arrays (MEAs). (frontiersin.org)
We recorded neuronal activity from multielectrode arrays as they were stepped through area MT. The set of recording sites in each array penetration described a plane parallel to the cortical layers. (jneurosci.org)
Our approach involves the use of multiple microelectrodes arranged in compact arrays and moved simultaneously parallel to the cortical laminas. (jneurosci.org)

A recent computational study confirmed that the ANEP dyes are affected only by the electrostatic environment and not by specific molecular interactions. (wikipedia.org)
To be able to explore neuronal and microcircuit dynamics, advanced imaging, electrophysiological techniques and computational models have been combined, allowing us to investigate neuronal ensembles activity and to connect microscale to mesoscale phenomena. (frontiersin.org)

We applied it with a new surgery, leaving the dura intact but allowing injection of large quantities of staining solution, and imaged cortical membrane potential oscillations with two-photon microscopy depth-resolved (25 to 100 µm below dura) in anesthetized and awake mice. (oist.jp)

which allows imaging in the scattering environment of the intact brain ( Denk and Svoboda, 1997 ). (jneurosci.org)

One way to record action potentials is to use high-impedance extracellular electrodes that are advanced into brain tissue and placed directly next to a single neuron, allowing for the extracellular recording of action potentials through the electrode [ 2 - 6 ]. (hindawi.com)

One obvious problem is that optical signals cannot be recorded from cortical regions that are buried within sulci. (jneurosci.org)
The headstage also provides an integrated stimulator, which can generate current or voltage stimulation signals on three independent channels (two for MEA2100-256-Systems) per MEA slot. (multichannelsystems.com)
Both models implement a delay-and-compare mechanism where two input signals from neighboring image pixels interact in a nonlinear way after one of them has been delayed with respect to the other. (elifesciences.org)

In our studies on neurotransmitter metabolism we have focused our efforts on transporters, a functional class of proteins that move neurotransmitters and other small molecules across membranes in cells. (stanford.edu)
These optogenetic techniques achieve unprecedented precision by introducing light-sensitive proteins into specific cell populations, which generate a response when illuminated. (electrooptics.com)
The most popular approach is to implant optical devices into the brains of freely moving animals, but the scattering and absorption of light in neural tissue requires an optical platform that provides enough power to excite the light-sensitive proteins, as well as the spatial resolution needed to stimulate specific regions of interest. (electrooptics.com)
Optogenetics allows neuroscientists to study neural processes by using an optical system to activate cell populations tagged with light-sensitive proteins. (electrooptics.com)

Antic & Zecevic 1995 J Neurosci 15:1392 and http://www.scholarpedia.org/article/Voltage-sensitive_dye ). (scholarpedia.org)

Aguirre AD, Chen Y, Fujimoto JG, Ruvinskaya L, Devor A, Boas DA (2006) Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography. (springer.com)
However, they also found that a single-fibre solution is unable to provide enough light to stimulate light-sensitive biomolecules located several millimetres away from the cerebral cortex. (electrooptics.com)

A larger voltage exerts a greater force, which moves more electrons through the wire at a given rate of time. (medscape.com)

Research projects focused on imaging neuronal activity in the brain of awake mice. (oist.jp)

Measurements of brain activity using very similar protocols have been made using metrics ranging from single-unit responses to functional imaging in both animals and humans. (visionsciences.org)
The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)

Here, NICE theory is shown to provide a detailed predictive explanation for the ability of ultrasonic (US) pulses to also suppress neural circuits through cell-type-selective mechanisms: according to the predicted mechanism T-type calcium channels boost charge accumulation between short US pulses selectively in low threshold spiking interneurons, promoting net cortical network inhibition. (eneuro.org)

For extracellular recording, the action potential voltage can be as low as just a few microvolts, making it challenging to record it reliably against various sources of noise. (hindawi.com)

Here we show that naive mice readily learned to detect brief trains of action potentials (five light pulses, 1 ms, 20 Hz). (nature.com)

The core material for imaging brain activity with voltage-sensitive dyes are the dyes themselves. (wikipedia.org)
Overall, the development of cortical activity under these conditions was accelerated. (frontiersin.org)
Akkin T, Dave DP, Milner TE, Rylander HG (2004) Detection of neural activity using phase-sensitive optical low-coherence reflectometry. (springer.com)
During periods of activity, the membrane potential depolarizes and subsequently repolarizes over a period of about one to several milliseconds [ 1 ]. (hindawi.com)
Choosing electrodes with suitable impedances makes the amplifier design appropriate for recording local field potential, which results from the activity of small neural networks, or appropriate for recording activity from a single neuron extracellularly. (hindawi.com)

Comparisons of responses from adjacent electrodes at successive locations enabled electrophysiological imaging of the two-dimensional pattern of preferred directions across the cortex. (jneurosci.org)
In addition, our results provide electrophysiological confirmation of map features that have been documented in other cortical areas and primate species by optical imaging. (jneurosci.org)
These limitations motivated us to develop an electrophysiological approach that is complementary to optical imaging. (jneurosci.org)

To study layer 1 membrane voltage oscillations, we synthesized a new voltage-sensitive dye, di1-ANNINE-6plus, that can diffuse into tissue. (oist.jp)

We used this model to explore the mechanisms underlying two sets of published data involving high frequency trains of action potentials, and the local generation of sodium spikelets. (yale.edu)

It also makes possible the measurement of spatial and temporal variations in membrane potential along the surface of single cells. (wikipedia.org)

Such multisite recordings may provide precise information on action potential initiation and propagation (including direction and velocity), and on the entire branching structure of a neuron. (wikipedia.org)

Using a voltage-sensitive-dye-based imaging method, we examined and compared the postnatal development of AAF and A1 in mice from postnatal day 11 (P11) to P40. (bvsalud.org)

Most dynamic sodium imaging experiments in brain slices have used standard fluorescence imaging technologies, ie, an arc lamp epifluorescence light source, a filter cube matched to the indicator, and a CCD camera to detect the change in fluorescence. (eneuro.org)

In certain preparations the pharmacological effects of the dyes may be completely reversed by removing the staining pipette and allowing the neuron 1-2 hours for recovery. (wikipedia.org)
We are pursuing these goals through molecular and biochemical studies, and, in collaboration with the Huguenard and Prince labs, through physiological and biosensor based imaging studies to better understand how pharmacological targeting of these molecules will influence neurological disorders. (stanford.edu)

The results support the initial hypothesis that this kind of ELEMF stimulation could be a treatment option for specific indications with promising potential for CNS applications, especially for degenerative diseases, such as Alzheimer's disease and other dementias. (frontiersin.org)
Recently, intramembrane cavitation within the bilayer membrane was proposed to underlie both the biomechanics and the biophysics of acoustic bio-effects, potentially explaining cortical stimulation results through a neuronal intramembrane cavitation excitation (NICE) model. (eneuro.org)
Our new theoretical analysis reveals that ultrasound can selectively excite different cortical neuron subtypes simply by changing the stimulation pattern, driven by the response properties of T-type calcium channels. (eneuro.org)

Human cortical areas can be mapped using fMRI, ECoG and EEG. (visionsciences.org)
Contrast is a key visual variable in that it controls visibility and measurements from single-units to optical-imaging to fMRI find general consistency in that cortical visual areas respond in monotonically increasing functions to increases in contrast. (visionsciences.org)

Inhibits release of glutamate and inhibits voltage-sensitive sodium channels, leading to stabilization of neuronal membrane. (medscape.com)

We had previously found that upward-sensitive T4 cells implement both preferred direction enhancement and null direction suppression (Haag et al. (elifesciences.org)

Dynamic calcium imaging is a major technique of neuroscientists. (eneuro.org)

0 B48 Fig. Action potentials are therefore sometimes called spike potentials. (forextradingwiz.com)

Articles, abstracts, and book chapters citing Globals Software (Globals Unlimited, Globals for Spectroscopy, and Globals for Images aka SimFCS) by authors not affiliated with the Laboratory for Fluorescence Dynamics (LFD). (uci.edu)

It has been applied to measure the action potentials of a single t-tubule of cardiomyocytes by Guixue Bu et al. (wikipedia.org)
1 Closed Shells and Magic Numbers We will start from the assumption that the interaction between one nucleon and all the other nucleons in the nucleus can be approximated by a suitable single-particle potential. (forex-consultant.com)

These results further support a unifying hypothesis for ultrasonic neuromodulation, highlighting the potential of advanced waveform design for obtaining cell-type-selective network control. (eneuro.org)

Methods: The molecular layer of the DG (dentate gyrus) of human epileptic tissue and rat nonepileptic tissue was electrically stimulated and the evoked responses were recorded with voltage-sensitive dye imaging to characterize the spatiotemporal properties. (pdffox.com)
High power intensities are needed to penetrate large regions of the brain, and Monte Carlo simulations by Frederic Pain and colleagues at the Université Paris Saclay suggest that fibres with large diameters and numerical apertures can deliver the maximum amount of power without causing any thermal damage to the cortical tissue. (electrooptics.com)

Imaging in Neuroscience: A Laboratory Manual provides the definitive collection of methods in use in this groundbreaking field. (cshlpress.com)
Imaging in Neuroscience: A Laboratory Manual is an essential guide to discovering and implementing these techniques. (cshlpress.com)

Voltage sensitive dyes are supplied by many companies who offer fluorescent probes for biological applications. (wikipedia.org)

The chromophore is believed to undergo a large electronic charge shift as a result of excitation from the ground to the excited state and this underlies the putative electrochromic mechanism for the sensitivity of these dyes to membrane potential. (wikipedia.org)
This orientation assures that the excitation induced charge redistribution will occur parallel to the electric field within the membrane. (wikipedia.org)

Chen WR, Shen GY, Shepherd GM, Hines ML, Midtgaard J (2002) Multiple modes of action potential initiation and propagation in mitral cell primary dendrite. (yale.edu)

We found that backpropagating action potentials (bAPs) are predominantly controlled by voltage-gated sodium and A-type potassium channels. (yale.edu)

It would help if the authors could provide a reason for the discrepancy between theory and experiment such as differences in specific membrane properties. (scholarpedia.org)

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