Copy plasmids. Medical search. Frequent questions

Hello, everyone: Does any one know how to recover one copy yeast plasmid using competent E. coli cell, or how to separate the plasmid DNA from yeast genomic DNA? (bio.net)
Twenty ESBL producing strains (15%) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 7), Klebsiella oxytoca (n = 2) and Enterobacter aerogenes (n = 2) were detected and further analyzed for their resistance transfer features, plasmid profile and nature of the resistance genes. (scielo.br)
Endotoxins, also known as lipopolysaccharides or LPS, are cell-membrane components of Gram-negative bacteria such as E. coli and are released during the lysis step of plasmid purification. (qiagen.com)
This plasmid is designed to express tagged proteins in E. coli. (sigmaaldrich.com)
The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. (sigmaaldrich.com)
While some plasmids were fully integrated, some were maintained as episomes and could be purified and retransformed into E. coli. (oregonstate.edu)
Although clonal expansion events appear to have contributed to the spread of particular CTX-M variants, such as bla CTX-M-15 within E. coli strain O25:H4 - ST131:05 ( 5 , 6 ), plasmids with the ability to spread efficiently, or epidemic plasmids, also are believed to be responsible for disseminating CTX-M ESBLs ( 7 ). (cdc.gov)
The plasmid spread to unrelated E. coli isolates within an index cattle farm and persisted within the environment. (cdc.gov)
In this study, a bla CTX-M-2 -harboring plasmid (pYD786-1) belonging to the sequence type 2 (ST2)-IncHI2 plasmid type and isolated from an Escherichia coli ST410 clinical strain was sequenced and analyzed. (ox.ac.uk)
Examples of plasmids : Puc 8 ( E.cli ), R-1, R-6, Col E1 ( E.coli ), Tol ( Pseudomonas putida ). (biotechfront.com)
F plasmid of E.coli. (biotechfront.com)
The AxyPrep MAG Plasmid purification kit utilizes a magnetic beads-based technology for high throughput purification of plasmid DNA from Escherichia coli cells. (krackeler.com)
The researchers reasoned that the method could be used to regulate an entire suite of genes and promoters, and tested their idea using a previously constructed circuit to produce sustained cycling of DNA plasmid concentration across a colony of E. coli cells. (sciencedaily.com)
Whether a vector is kept at low copy number, low culture density, or culture overgrowth, this guide will help you navigate your purification woes and determine the best way to boost your plasmid yields from E. coli cultures. (zymoresearch.com)
Sometimes particular E. coli strains are sub-optimal for plasmid extraction. (zymoresearch.com)
When possible, stick to tried and tested strains like E. coli DH5α which contain mutations to lack certain endonucleases and increase plasmid stability. (zymoresearch.com)
Thus, these modified E. coli strains are used as workhorses for molecular cloning and plasmid production. (zymoresearch.com)
There are a couple ways to ensure high yield plasmid preparations from E. coli cultures by using antibiotics for selective pressure. (zymoresearch.com)
Chloramphenicol treatment can stop protein production but allow the E. coli to continue to "amplify" the plasmids, resulting in increased yields during plasmid purification 1 . (zymoresearch.com)
Title : Changing plasmid types responsible for extended spectrum cephalosporin resistance in Escherichia coli O157:H7 in the United States, 1996-2009 Personal Author(s) : Folster, J. P.;Pecic, G.;Stroika, S.;Rickert, R.;Whichard, J. (cdc.gov)

Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its replication origin ( oriV ). (sri.com)
We explored the utility of a broad host-range plasmid, pBBR1MCS-4, to transform chlamydiae, with a goal of simplifying the transformation process. (oregonstate.edu)

This altered shape prevents RNA II from hybridizing to the DNA and being processed from RNaseH to produce the primer necessary for initiation of plasmid replication. (wikipedia.org)
DNA sequence which encode initiation of plasmid replication by recruiting bacterial transcription machinary for replication enzymes & proteins. (biotechfront.com)

The sequence was a 93,629-bp plasmid encoding a single antimicrobial drug resistance gene, bla CTX-M-14 . (cdc.gov)
is useful as the sequences did not match any known plasmid sequence deposited in public databases. (frontiersin.org)
From the sequence analysis, a single mutation (C→T) at position 4690 was found, and we confirmed that this single mutation was responsible for the increased plasmid copy number. (biomedcentral.com)
It isn't clear whether the presence of the SV40 sequence in the plasmid DNA of mRNA vaccines was identified by Pfizer when the company applied for approval from the FDA. (zerohedge.com)
The researchers used an endonuclease from Saccharomyces cerevisiae, a species of yeast, expressed alongside a plasmid containing the nuclease recognition sequence to temporarily reduce the plasmid's copy number below natural levels. (sciencedaily.com)

Thus, there is a big demand for high-copy-number plasmids for useful gene manipulation and overproduction of recombinant proteins in Leuconostoc . (biomedcentral.com)

Some of the most clinically relevant resistance genes, such those encoding carbapenemases (ß-lactamase enzymes able to degrade carbapenem antibiotics), are carried on conjugative plasmids that spread across high-risk bacterial clones 5 , 6 . (nature.com)
Most of these plasmids were detected to carry both TEM- and SHV-derived genes by PCR, and confirmed by localizing each gene by hybridization assay. (scielo.br)
Genes that confer resistance to antimicrobial drugs used in human or veterinary medicine are often mobilized on plasmids. (cdc.gov)
The ability and frequency with which antimicrobial resistance genes disseminate between bacteria in humans, the environment, and animals is still debated, and the role of plasmids in this movement between ecosystems, including the food chain, is also still contested, despite mounting evidence that it occurs ( 8 , 9 ). (cdc.gov)
Replication genes were not identified in some plasmids, a situation that has led to the possibility of host interaction involvement. (frontiersin.org)
Some plasmids were also void of par genes and intriguingly, repA gene was also not discovered in these plasmids. (frontiersin.org)
Plasmid stabilization/stability protein-encoding genes were observed in some plasmids but were not established for participating in plasmid segregation. (frontiersin.org)
Conjugation genes were identified portraying the conjugation ability amongst Pandoraea plasmids. (frontiersin.org)
Additionally, we found a shared region amongst some of the plasmids that consists of conjugation genes. (frontiersin.org)
The identification of genes involved in replication, segregation, toxin-antitoxin systems and conjugation, would aid the design of drugs to prevent the survival or transmission of plasmids carrying pathogenic properties. (frontiersin.org)
Additionally, genes conferring virulence and antibiotic resistance were identified amongst the plasmids. (frontiersin.org)
but genes carried by plasmids usually encodes traits beneficial for bacteria. (biotechfront.com)
these genes provide a survival advantage to the bacterial host thet allows for selection of plasmid containing bacteria. (biotechfront.com)
This plasmids carry genes thet provide resistance to one or more antibiotics and poisons. (biotechfront.com)
This plasmid carry genes that codes for colicins proteins that kill other bacteria. (biotechfront.com)
This types of plasmid carry genes that allow the host bacterium to metabolize unusual substances like Toluene and salicylic acid. (biotechfront.com)
This is an intermediate class of plasmid carry only a subset of genes (mob+) required for transfer. (biotechfront.com)
In an Aug. 8, 2019, patent filed by Moderna regarding RNA vaccines, the company states that the direct injection of naked plasmid DNA into a living host comes with "potential problems, including the possibility of insertional mutagenesis, which could lead to the activation of oncogenes or the inhibition of tumor suppressor genes. (zerohedge.com)

QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. (qiagen.com)
The QIAfilter Plasmid Kits combines QIAfilter Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tips containing anion-exchange resin for efficient plasmid purification. (qiagen.com)
QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour. (qiagen.com)
The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment. (qiagen.com)
Sometimes, plasmid purification just doesn't go according to plan. (zymoresearch.com)
The efficacy of column-based plasmid purification doesn't just depend on how much plasmid is loaded onto the column, but also the total amount of biomass being loaded as well. (zymoresearch.com)
If you want to scale up your plasmid purification, try using a kit designed for high inputs of culture, such the ZymoPURE II Midipreps and Maxipreps , which can process more culture. (zymoresearch.com)
Purification from cultures grown in highly enriched media such as Terrific Broth (TB) yield higher amounts of plasmid DNA per ml of culture than standard Luria-Bertani Broth (LB). (zymoresearch.com)
One method for plasmid amplification uses an inhibitory amount of chloramphenicol (170 µg/ml) added to a culture, which is then incubated further until plasmid purification (typically the next day) 2 . (zymoresearch.com)
A variation of this method that reports higher plasmid yield uses lower amounts of chloramphenicol (10-20 µg/ml) added to exponentially growing cells that are subsequently incubated overnight prior to plasmid purification 3 . (zymoresearch.com)

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. (sigmaaldrich.com)

QIAfilter Cartridges , provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. (qiagen.com)

EcoRI restriction enzyme analyses of R plasmids were used in the detection of epidemic plasmids. (scielo.br)

This delivers the convenience of high plasmid DNA yields but the additional burden of the high copy number restricts the plasmid size. (wikipedia.org)
Yields of up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), and 100 µg (midi) transfection-grade, high-copy plasmid DNA are achieved from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). (qiagen.com)
The upstream manufacturing process is an important part of bringing plasmid DNA (pDNA) materials to market, as achieving the highest yields possible at large scale is essential to meeting future demand. (biopharminternational.com)
Sometimes the simplest way for how to increase plasmid yields is to just input more raw material. (zymoresearch.com)
If you are experiencing low yields for your plasmid prep, double check that the strain you're using is best for plasmid propagation. (zymoresearch.com)

QIAGEN Plasmid Plus technology delivers the same performance and quality as anion-exchange technology. (qiagen.com)
The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. (qiagen.com)

Incorporating our fitness results into a simple population dynamics model revealed a new set of conditions for plasmid stability in bacterial communities, with plasmid persistence increasing with bacterial diversity and becoming less dependent on conjugation. (nature.com)
The multicopy 2-micron plasmid of Saccharomyces cerevisiae, a resident of the nucleus, is remarkable for its high chromosome-like stability. (tau.ac.il)

It is used as an initiation point for PCR amplification or sequencing of the plasmid. (biotechfront.com)
A decrease in plasmid population due to rare missegregation events is rectified by plasmid amplification via a recombination-induced rolling circle-like replication mechanism. (tau.ac.il)
Appropriate plasmid amplification, without a runaway increase in copy number, is ensured by positive and negative regulation of FLP gene expression by plasmid-coded proteins and by the control of Flp level/activity through host-mediated posttranslational modification(s) of Flp. (tau.ac.il)

The kit has been used to purify plasmid DNA for high efficiency transfection. (sigmaaldrich.com)
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. (primelabmed.com)

therefore, dissemination has been attributed to conjugative plasmids rather than to clonal expansion of a bacterial host strain ( 13 ). (cdc.gov)

pYD786-1 belongs to the APEC-O1-R-type IncHI2 plasmids, which are widely distributed in human, poultry, and livestock strains. (ox.ac.uk)
The second method uses chloramphenicol, an antibiotic that halts protein synthesis and decouples it from plasmid replication, when culturing strains containing a plasmid with a relaxed origin of replication. (zymoresearch.com)

Those include an antibiotic-free plasmid maintenance system, operator repressor titration (ORT), and cell-lysis technologies ( 2 - 4 ). (bioprocessintl.com)
In the context of plasmid bioproduction, however, it is also common to include cell lysis and clarification as part of the upstream process, according to Nuria Gomez Santos, head of process and analytical development for pDNA with Catalent Cell & Gene Therapy. (biopharminternational.com)
Given the importance of lysis, neutralization, and clarification in pDNA production, John Bowen, senior director for Nucleic Acids and Plasmid Operations at The Center for Breakthrough Medicines, agrees that these steps should be thought of separately, as mid-stream unit operations. (biopharminternational.com)

Second, studies tend to analyse the fitness effects of a single plasmid in a single bacterium. (nature.com)
These results suggest that the inability of the DnaA protein of a host bacterium to form a stable and functional complex with the DnaA boxes at oriV is a limiting step for plasmid host range. (sri.com)
Bacterial plasmids are key vectors of horizontal gene transfer, mediating the mobilization of genetic material from 1 bacterium to another. (cdc.gov)
A. Conjugative plasmid - This plasmid contain tra gene for conjugation and transfer of plasmid to another bacterium (tra+, mob+). (biotechfront.com)

Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. (qiagen.com)
High-yield protocols and extra buffer volumes are provided with the QIAGEN Plasmid Plus Kits. (qiagen.com)
Increasing the amount of culture processed leads to higher total yield of plasmid DNA if kit parameters are adjusted accordingly to accommodate higher biomass. (zymoresearch.com)
Additionally, total plasmid yield can vary depending on the type of culture broth used, typically Luria-Bertani broth (LB) or Terrific Broth (TB). (zymoresearch.com)
Don't be afraid to experiment with your growth conditions to see which gives you the best plasmid yield. (zymoresearch.com)
Alternatively, another study demonstrated increased plasmid yield by growth in the presence of sub-inhibitory concentrations of chloramphenicol (3-5 µg/ml) from the time of culture inoculation until plasmid was harvested the next day 4 . (zymoresearch.com)

Many biotechnology applications utilize mutated plasmids that replicate to high copy number. (wikipedia.org)
These examples do not necessarily replicate plasmid fitness effects in natural bacterial hosts, which remain largely unexplored. (nature.com)
Plasmid is a short, naturally occurring extra chromosomal , usually circular, double stranded DNA molecule that replicate, autonomously and lead an independent existence in Bacterial cell. (biotechfront.com)

Relaxed plasmids are generally regulated through one of two mechanisms: antisense RNA or iteron binding groups. (wikipedia.org)
Iteron plasmids, including F and RK2-related plasmids, have oriV regions containing multiple (~3-7) repeats of 17-22 bp iteron sequences.pSC101 represents a simple model of an iteron plasmid. (wikipedia.org)
Iteron plasmids control copy number through two combined methods, suitable for low copy number stringent plasmids. (wikipedia.org)
It was found that 45-bp deletion abolished binding of a plasmid replication protein to the plasmid DNA, supporting an iteron model for plasmid replication. (readabstracts.com)

In this study, we determined the fitness effects of the major antibiotic resistance plasmid pOXA-48_K8 in wild-type, ecologically compatible enterobacterial isolates from the human gut microbiota. (nature.com)
Hence, the spread of virulence factors and antibiotic resistance properties caused by plasmids is possible, leading to a threat in public health. (frontiersin.org)
It's imperative to ensure the correct amount of antibiotic is present in your culture, otherwise the lack of selection pressure will cause your culture to start to dilute out the plasmid during cell division. (zymoresearch.com)

For example, the F plasmid, which is the origin of BACs (bacterial artificial chromosomes) is a single copy plasmid with a partitioning system encoded in an operon right next to the plasmid origin. (wikipedia.org)

Plasmids are extra-chromosomal mobile genetic elements able to transfer between bacteria through conjugation 1 . (nature.com)
From this information, PCRs identifying novel features of pCT were designed and applied to isolates from several countries, showing that the plasmid has disseminated worldwide in bacteria from humans and animals. (cdc.gov)
Complete DNA sequences can be used as a platform to develop rapid epidemiologic tools to identify and trace the spread of plasmids in clinically relevant pathogens, thus facilitating a better understanding of their distribution and ability to transfer between bacteria of humans and animals. (cdc.gov)
Plasmids are one of the primary sources for extrachromosomal DNA in bacteria and are capable of self-transmission. (frontiersin.org)
The isolated plasmid could be a useful genetic tool for high-level gene expression in Leuconostoc , and for extending the applications of this useful bacteria to various areas in the dairy and pharmaceutical industries. (biomedcentral.com)
A plasmid is a strand of circular DNA that is common to bacteria and specific parasites. (zerohedge.com)
It is considered particularly worrisome because it is located on a plasmid, rather than a chromosome, and thus can be readily transferred to other bacteria. (medscape.com)

PCR and PacBio-based whole genome sequencing were used to determine if the plasmid was maintained within the chromosome or as an episome. (oregonstate.edu)
PacBio sequencing of the cloned transformants revealed allelic exchange events between the chromosome and plasmid pBVR2 that replaced chromosomal incA with the plasmid GFP:CAT cassette. (oregonstate.edu)
The data also showed evidence of full integration of the plasmid into the bacterial chromosome. (oregonstate.edu)
Plasmid is naturally found in cytoplasm, seperately from the main bacterial chromosome and are much smaller incomparision. (biotechfront.com)
Plasmids are normally in size range of 1kb to 250 kb which is smaller much smaller than Bacterial chromosome. (biotechfront.com)
[4] Before this happens, each chromosome is duplicated ( S phase ), and both copies are joined by a centromere , resulting either in an X-shaped structure (pictured above), if the centromere is located equatorially, or a two-arm structure, if the centromere is located distally. (wikipedia.org)
Because ET-A is chromosome borne and ET-B is plasmid borne, multiple intracellular copies of ET-B may exist, which would lead to higher ET-B production. (medscape.com)

This plasmid incapable of initiating conjugation , hence can only be transferred with the help of conjugative plasmid ( tra- , mob- ) under some circumstances. (biotechfront.com)

Current evidence supports a model in which the Rep-STB system promotes the physical association of the plasmid with chromosomes and thus plasmid segregation by a hitchhiking mechanism. (tau.ac.il)

ABSTRACT The extended-spectrum β-lactamase gene bla CTX-M-2 is mainly associated with IS CR1 embedded in complex sul1 -type integrons, but information on the genetic context of plasmids harboring the IS CR1-bla CTX-M-2 module remains limited. (ox.ac.uk)
However, due to limited genetic tools including plasmids for Leuconostoc , there has not been much extensive research on the genetics and engineering of Leuconostoc yet. (biomedcentral.com)
In particular, expression plasmids are one of the most fundamental systems for genetic manipulations and protein production, especially to carry foreign DNA and to produce recombinant proteins in a host. (biomedcentral.com)
Such ancillary materials include plasmid DNA used as a template for the production of mRNA and for the production of viral vectors used for delivery of genetic cargo. (biopharminternational.com)

The FDA's 2009 "Guidance on Prophylactic DNA Vaccines: Analysis and Recommendations" states that concerns about plasmid DNA potentially integrating into the genome of the vaccine recipient and increasing the likelihood of issues like "malignant transformation, genomic instability, or cell growth dysregulation" were raised when DNA vaccines were initially introduced for clinical use. (zerohedge.com)

Get in touch with our team today and request a quote for your QIAGEN Plasmid Plus Midi Sample Kit (5) trial kit. (qiagen.com)
QIAGEN Plasmid Plus Kits are intended for molecular biology applications. (qiagen.com)
The QIAGEN Plasmid Plus Kits also feature a dedicated wash buffer for endotoxin reduction. (qiagen.com)
The unique binding chemistry of the QIAGEN Plasmid Plus columns ensures that pure plasmid DNA is obtained every time. (qiagen.com)
no. 12181) and the QIAGEN Plasmid Giga Kit (cat. (qiagen.com)
Visit the QIAGEN plasmid kits web page to learn more. (qiagen.com)
no. 19781) with the QIAGEN Plasmid Mega Kit (cat. (qiagen.com)
Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. (qiagen.com)

It have no characteristic beyond the ability to promote conjugal transfer of plasmids. (biotechfront.com)

For example, pBR322 is a medium copy number plasmid (~20 copies/cell) from which several high copy number cloning vectors (>100 copies/cell) have been derived by mutagenesis, such as the well known pUC series. (wikipedia.org)
Larger high copy plasmids (>30kb) are disfavoured and also prone to size reduction through deletional mutagenesis. (wikipedia.org)
Using an existing low-copy plasmid, the copy number of plasmid was increased by random mutagenesis followed by FACS-based high-throughput screening. (biomedcentral.com)
DNA constructs feature the mosaic analysis with the dual-recombinase-mediated cassette exchange (MADR) method, allowing for single-copy, somatic mutagenesis of driver mutations. (bvsalud.org)

This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. (sigmaaldrich.com)
The effectiveness of the isolated high-copy-number plasmid for the overproduction of recombinant proteins was successfully demonstrated with two protein models Glutathione-S-transferase (GST) and α-amylase. (biomedcentral.com)
A partitioning system comprised of two plasmid-coded proteins, Rep1 and Rep2, and a partitioning locus, STB, is responsible for equal or nearly equal segregation of plasmid molecules to mother and daughter cells. (tau.ac.il)

The plasmid may be viewed as a highly optimized selfish DNA element whose genome design is devoted entirely to efficient replication, equal segregation, and copy number maintenance. (tau.ac.il)

The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines. (qiagen.com)
The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription. (primelabmed.com)
This dual-plasmid system is suitable for storing, reading and rewriting various types of information, including text, codebooks and images. (phys.org)

Plasmid transformation requires a replicon derived from the native Chlamydia plasmid, and these transformations are species-specific. (oregonstate.edu)

Demand for plasmids is rising dramatically as large numbers of messenger RNA (mRNA) vaccines and viral-vector-based therapeutics advance through clinical trials toward approval and commercialization. (biopharminternational.com)
Plasmids for use in mRNA and viral-vector manufacturing may be intermediate- or GMP-grade, depending on the preference of the drug manufacturer. (biopharminternational.com)
Pfizer and Moderna COVID-19 mRNA vaccines "are contaminated with plasmid DNA fragments which have not been removed during the current manufacturing processes," the doctor wrote. (zerohedge.com)

Addgene is a nonprofit plasmid repository. (addgene.org)

A mechanism by which SCP2 derived plasmid achieves increased levels of metabolite production was identified to examine the 45-bp deletion mutation in the plasmid replication origin increased plasmid copy number. (readabstracts.com)

Most plasmids require a plasmid-encoded protein, usually called Rep, to separate the strands of DNA at the origin of replication (oriV) to initiate DNA replication. (wikipedia.org)
The synthesis of Rep protein is controlled in order to limit plasmid replication and therefore regulate copy number. (wikipedia.org)
It is made from the first promoter until the plasmid reaches its copy number, upon which the protein CopB represses this primary promoter. (wikipedia.org)
The higher the concentration of the plasmid, the more CopA RNA is produced and the less RepA protein can be synthesized, increasing inhibition of plasmid replication. (wikipedia.org)

Hasty and his team employed the endonuclease to reduce the number of these plasmids present in the colony and used this mechanism as negative feedback to driving the oscillations in gene expression. (sciencedaily.com)

Increased plasmid copy number is essential for Yersinia T3SS function and virulence. (cdc.gov)

Rep binds to specific DNA sequences in oriV which are unique to a plasmid type. (wikipedia.org)

Third, most mathematical models of plasmid population biology study clonal or near-clonal populations. (nature.com)

Plasmids are considered as replicon . (biotechfront.com)

Cobra Biologics has been producing plasmid DNA for a number of clinical applications for over 15 years at an approved site in the United Kingdom under the European Union (EU) clinical trials directive. (bioprocessintl.com)

By controlling DNA copy number, researchers can effectively regulate gene expression. (sciencedaily.com)

RNA I serves as a major plasmid-encoded inhibitor of this process whose concentration is proportional to plasmid copy number. (wikipedia.org)
More RNA I is produced as the plasmid concentration increases, which increasingly inhibits replication, resulting in regulation of copy number. (wikipedia.org)

The final plasmid construct, pBVR2, was successfully transformed into C. trachomatis strain L2-434. (oregonstate.edu)
In2 precursor, likely played a role in the generation of the MRR in pN13-01290_23, the putative progenitor plasmid of pYD786-1, found in a foodborne Salmonella strain. (ox.ac.uk)
Qualified cell banks should be used and tested for identity, viability, strain, genotype/phenotype, and presence/copy number of the plasmid vector of interest, along with a description of its structural elements. (bioprocessintl.com)

The use of Red/ET recombination to generate a large number of shuttle plasmids from a diverse library of chimeric domains for directed evolution of the pikromycin polyketide synthase (PKS) is reported. (readabstracts.com)

The high-copy number plasmid was successfully isolated by FACS-based high-throughput screening of a plasmid library in L. citreum . (biomedcentral.com)
It is used to select for cells that has successfully taken up plasmid for the purpose of expression of inserted DNA. (biotechfront.com)

Expression of ESBL is often associated with multidrug resistance and dissemination by resistance plasmids. (scielo.br)
Due to this characteristic, it can be used for rapid and low-cost data copy dissemination. (phys.org)

Some are interogative plasmid called episomes . (biotechfront.com)
F plasmid is also called episomes . (biotechfront.com)

The QIAfilter Plasmid Mega Kit (cat. (qiagen.com)

Plasmid persistence in bacterial populations is strongly influenced by the fitness effects associated with plasmid carriage. (nature.com)
To fully understand plasmid persistence in natural bacterial populations, it will be necessary to address these limitations. (nature.com)

Plasmid transfer assays were performed using broth mating techniques. (scielo.br)

These fitness costs make it difficult to explain how plasmids are maintained in bacterial populations over the long-term in the absence of selection for plasmid-encoded traits, a puzzle known as "the plasmid-paradox" 10 . (nature.com)

These results help to explain the high prevalence of plasmids in the greatly diverse natural microbial communities. (nature.com)
To solve the rewriting problem, Prof. Liu Kai from the Department of Chemistry, Tsinghua University, Prof. LI Jingjing from the Changchun Institute of Applied Chemistry (CIAC) of the Chinese Academy of Sciences, and Prof. Chen Dong from Zhejiang University led a research team that recently developed a dual-plasmid editing system for accurately processing digital information in a microbial vector. (phys.org)

But linear Plasmids are also reported in genera streptomycetes and Borrelia . (biotechfront.com)

Ti plasmid of Agrobacterium tumifaciens , which induce crown gall disease on dicot plants. (biotechfront.com)

Anatomy4

Organisms27

Diseases4

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes95

Information Science4

Health Care1

Coli20

Broad host range plasmid2

Initiation of plasmid replication2

Sequence5

High copy number plasmids1

Genes18

Purification10

Method for purifyi1

EndoFree Plasmid Kits1

Epidemic plasmids1

Yields5

Anion-exchange2

Stability2

Amplification3

Purify plasmid2

Conjugative plasmids1

Strains2

Lysis3

Bacterium4

Yield6

Replicate3

Iteron4

Antibiotic3

Artificial chromosomes1

Bacteria7

Chromosome7

Conjugation1

Chromosomes1

Genetic4

Integrating into the genome1

QIAGEN8

Conjugal1

Mutagenesis4

Proteins3

Segregation1

Suitable3

Transformation1

MRNA3

Addgene1

Replication origin1

Protein4

Mechanism1

Virulence1

OriV1

Clonal1

Replicon1

Biologics1

Gene expression1

Concentration2

Strain3

Shuttle1

Successfully2

Dissemination2

Episomes2

Mega Kit1

Persistence2

Assays1

Traits1

Microbial2

Genera1

Induce1