Conventional microfluidic analytical techniques. Medical search. Frequent questions

However, the lack of automation, repeatability and dynamic range of detection in conventional platforms such as FTIR, have been major limitations which have been addressed with the development of microfluidic modulation spectroscopy. (wikipedia.org)
Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for the immunodetection of medically relevant markers in biologic samples such as blood, urine, and saliva. (hindawi.com)
These issues can be overcome by using microfluidic platforms that require low sample volumes (eliminating the need for excessive dilution), allow rapid interface stabilization, minimize the wastage of organic solvents, and enhance the extraction efficiency of radioactive metal resulting from high interfacial area and small diffusional lengths. (aiche.org)
We developed polymer based microfluidic platforms over glass or silicon-based microfluidic platforms to enable simple and less involved fabrication procedure. (aiche.org)
The developed microfluidic platforms provided quantitative information on the extraction process compared to conventional techniques, due to the superior control over the spatial and temporal properties of the interface and enabled rapid extraction times and high extraction efficiencies owing to minimal diffusion lengths. (aiche.org)
This article will review historical bonding techniques still in use for manufacturing HPLC stationary phases today, and also examine some emerging technologies that may be able to tackle unmet needs in novel platforms and phase construction. (chromatographyonline.com)
Some automated platforms include new or alternative ELISA formats typically with miniaturised or microfluidic components. (ddw-online.com)
More recently, bead-based methods and microfluidic platforms have started to impact immunoassays and by coupling with single molecule detection look set to take the sensitivity of traditional ELISA to new levels. (ddw-online.com)
To investigate the potential of isoDEP for cell analysis, a comparison to existing dielectrophoresis-based electrokinetic techniques was encouraged, including electrorotation (ROT) microfluidic platforms. (louisville.edu)
This project consist in the design, manufacture and validation of microfluidic integrated plasmonic platforms based on 3D hierarchical nanoparticles assemblies and prove its efficiency in flow as photocatalysts and in the detection of biomolecules using surface enhanced Raman scattering spectroscopy. (uvigo.es)

Analogous to the channel dimensions, the gap height and the electrode side length are the two most important geometrical variables affecting droplet motion in digital microfluidic systems. (asme.org)
The first was a 2010 Analytical Chemistry paper detailing a digital microfluidic platform for multiplex real-time PCR using a closed-loop, flow-through format. (genomeweb.com)

Nanosensors, such as those based on titanium oxide nanowires or palladium nanoparticles are used for analytical detection of contaminants in water samples. (nanowerk.com)
This PhD thesis is framed in the context of the project entitled "Microfluidic-induced Hierarchical 3D assemblies of Metal nanoparticles for advanced catalytic and sensing applications" funded by the Spanish Ministry of Science, Innovation to the Functional NanoBiomaterials Group (former Colloid Chemistry Group). (uvigo.es)
Chapter 1 is a general introduction about plasmonic nanoparticles and their optical properties, strategies to fabricate plasmonic assemblies and the resultant optical properties, a basic introduction of the different characterisation techniques employed in the thesis, and the basic principles of Raman scattering and SERS. (uvigo.es)

Here we introduce a novel integrated analytical technology for photochemistry by microfluidic coupling of a HC-PCF nanoflow reactor to supplementary detection devices. (mpg.de)
Therefore, this study aims to develop a portable on-site/on-chip analysis system by evaluating combinations of microfluidic extraction and detection systems for target toxic radionuclides such as selenium, lanthanide, and actinides. (titech.ac.jp)
Two types of microfluidic devices consisting of parallel phase microchip and plug flow microtube were investigated, and optimal conditions for the extraction and detection performance for the target elements were identified. (titech.ac.jp)
Detection techniques for target elements included fluorescence microscopy and thermal lens spectrometry, and were also compared to conventional detection methods. (titech.ac.jp)
These results provided ample evidence for deploying microfluidic extraction and detection systems for use in elemental screening at Fukushima, achieving acceptable separation efficiencies above 90% for all cases in less than 30 seconds. (titech.ac.jp)
Based on latest progress relying on bio-analytical assays and micro-fluidic flow injection with TLM detection we have also witnessed firs routine applications of TLS in analytical and diagnostic laboratories, which on wine side actually classifies TLS as a conventional and routine analytical tool, but at the same time opens new horizons for development and applications of this ultrasensitive and rapid spectrometric technique. (ung.si)
Classical electrode-based DNA electrochemical biosensors with combined electrokinetic techniques present the advantage of being able to improve the working electrode's bioactive part during their fabrication and also the hybridization yield during the sensor detection phase. (ibecbarcelona.eu)
Either way, merging analytical detection technologies with microfluidics and cell culture is key to the development of these systems for advanced in vitro analysis. (selectbiosciences.com)
Moreover since multiple determinations of biomarkers have demonstrated to provide more accurate information than individual determinations to assist the clinician in prognosis and diagnosis, the detection of several clinical biomarkers by using the same analytical device hold enormous potential for early detection and personalized therapy and will simplify the diagnosis providing more information in less time. (mdpi.com)
Due to the increasing demand for rapid and accurate detection of pathogens, molecular techniques have gained popularity in the nutrition industry in recent years. (grandviewresearch.com)
Biosensors are compact analytical devices that are portable systems used for the detection of food-related microorganisms. (grandviewresearch.com)
The detection restrict of this digital methodology for N protein was 33.28 pg/mL, which was 300 instances decrease than conventional double-antibody sandwich primarily based enzyme-linked immunosorbent assay (ELISA). (humgen.org)
Surface-enhanced Raman scattering (SERS) is a vibrational spectroscopic technique known to provide ultrasensitive detection and quantitative analysis of molecules located on or nearby a plasmonic surface, even down to the single-molecule regime. (uvigo.es)
Several new TB diagnostic tests have recently been introduced, based on different detection techniques (e.g. (cdc.gov)

Analytical Chemistry , 93 (13) , 5503 -5512. (swansea.ac.uk)
These features convert SERS into a promising tool for its implementation in diverse areas as biomedicine, environmental chemistry, analytical chemistry, and, overall, sensing applications. (uvigo.es)

The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. (hindawi.com)
Cellix' VenaFlux™ platform, reported to be the first semiautomated, high-throughput microfluidic cell-based assay system, measures cells' adhesion to antibody-coated or endothelial cell cultured microchannels, mimicking physiological flow. (genengnews.com)

Applying a continuous flow through the fiber, we deliver photochemical reaction products to a mass spectrometer in an online and hence rapid fashion, which is highly advantageous over conventional cuvette-based approaches. (mpg.de)
Although new synthetic approaches have been applied to reversed-phase ligands, the bulk of the commercially available phases are still manufactured via conventional methods (6). (chromatographyonline.com)
Several examples of analytical approaches for monitoring liver slice behavior stemming from our labs will be presented in this presentation. (selectbiosciences.com)
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
The increasing awareness of the importance of water for human and environmental health has prompted the need for developing fast, user-friendly and cost-effective analytical approaches for quality control and management. (researchgate.net)

Conventional flow cytometer has the advantage of high throughput: detect single cell properties at rates from hundreds to 100,000 cells per second. (e-consystems.com)

Nanowerk News ) Nanotechnology refers to a broad range of tools, techniques and applications that simply involve particles on the approximate size scale of a few to hundreds of nanometers in diameter. (nanowerk.com)
The impurities that nanotechnology can tackle depend on the stage of purification of water to which the technique is applied, the team adds. (nanowerk.com)

Here we demonstrate this method to create self-enclosed microfluidic devices with a few simple steps, in a number of flexible and transparent formats. (nature.com)
Structural colour, a property of organized microfibrillation, becomes an intrinsic feature of these microfluidic devices, enabling in-situ sensing capability. (nature.com)
Inevitably, the process of creating a complex channel, and then sealing its open surface against another material to create an enclosed channel, is a significant practical obstacle to advances in microfluidic devices. (nature.com)
a Conventional lithographic techniques for microfluidic devices require a sealing step, as shown by the schematic. (nature.com)
Microfluidic devices also called μ TAS (micro total analysis systems) or lab-on-a-chip systems belong to the most promising technologies studied in this context. (hindawi.com)
Microfluidic systems used in LOC devices allow the manufacture of millions of microchannels, each measuring mere micrometers. (adejournal.com)
To streamline analysis processes for waste characterization, simple and portable analytical devices in and around the decommissioning site have been required. (titech.ac.jp)
Lab-on-chip (LoC) devices embody several key features, including the use of small sample volume, control over fluid dynamics, easy integration of cell manipulation techniques (cell sorting, cell isolation) and higher throughput than conventional analytical methods (e.g. patch clamp) [1-2]. (polito.it)
Microfluidic Devices for Continuous Chromatography of Biologics. (codobio.eu)
In this field, electrochemical immunosensors have demonstrated to offer interesting alternatives against conventional strategies due to their simplicity, fast response, low cost, high sensitivity and compatibility with multiplexed determination, microfabrication technology and decentralized determinations, features which made them very attractive for integration in point-of-care (POC) devices. (mdpi.com)
For instance, the use of microfluidic-based devices requires far lower levels of reagents than conventional methods. (labmanager.com)
For instance, we developed innovative solutions to improve the encapsulation of flowing particles using viscoelastic flows in microfluidic devices, and we also developed microfluidic techniques to evaluate rheological parameters not measurable via conventional techniques. (swansea.ac.uk)
Controlled viscoelastic particle encapsulation in microfluidic devices. (swansea.ac.uk)
A Review of Microfluidic Devices for Rheological Characterisation. (swansea.ac.uk)
We will also present work on the effects of non-invasive electrical stimulation on bladder overactivity following spinal cord injury, looking to translate successful techniques into practicable, wearable devices for long-term use. (gla.ac.uk)
Paper-based analytical devices (PADs) are considered a key solution, as they provide a low-cost platform for developing PON biosensors for cancer biomarker detections. (hokudai.ac.jp)

Compared to conventional immunoassays microfluidic systems offer efficient mass transport and a reduced surface to volume ratio. (hindawi.com)

The development and analytical applications of electrochemical sensors based on antimony tin oxide (ATO)-Prussian blue (PB) screen-printed electrode (SPE) and PEDOT-PB modified glassy carbon electrode are presented. (researchgate.net)
This work reports the fabrication and analytical assessment of a very simple sample-to-result electrochemical sensor for discrete. (researchgate.net)

In 2009, our group developed a novel microfluidic diagnostic device combining REA technology with an innovative fluidic setup operating by passive capillary force. (hindawi.com)
Most recently, a study published in the European Journal of Clinical Microbiology and Infectious Diseases showed the microfluidic device was able to detect C. albicans DNA in low volumes of blood. (genomeweb.com)
In this work, a novel LoC device is proposed, which integrates in-channel IFC and in-chamber MEAs in the same microfluidic platform. (polito.it)
The performance of the microfluidic device is tested in a fluidic system with microbeads of different sizes (4-6-8 μm). (polito.it)
Lab2Lab thus provides on-demand delivery of a single sample tube to a sampling device, then to the analytical hardware, and then to a waste container or collection point, explained Sanghera. (genengnews.com)
Initial results indicated that for a particle suspended in 10 mS/m media, if the channel width and height are below 10 particle diameters, the electrothermal-driven flow is reduced by ∼ 500 times compared to the 500 µm thick conventional isoDEP device. (louisville.edu)
Due to isoDEP device limitations and other complications, other techniques were pursued to electrically characterize phytoplankton cells in suspension. (louisville.edu)

BioScience Innovations provide any kind of reagents, and aims to select the best source of conventional reagents (buffers, saturating agents, ELISA substrates,…), as well as to promote great specific, original and powerful solutions. (interchim.com)

For reasons mentioned previously, microfluidic modulation spectroscopy provides the sample capacity through 96 well plate operation and technical capabilities to elucidate colloidal and chemical stability, lacking in existing techniques such as size exclusion chromatography (SEC), mass spectrometry and capillary electrophoresis. (wikipedia.org)
Within the current research, a full analytical workflow has been developed primarily based on using varied chromatographic modes: measurement exclusion chromatography (SEC), ion change chromatography (IEX), reversed section liquid chromatography (RPLC), and hydrophilic interplay chromatography (HILIC), all mixed with excessive decision mass spectrometry (MS). (humgen.org)

It was the fact that further miniaturization of chromatography for bioprocess development with simple and cost-effective microfabrication techniques such as micromilling technology is possible. (codobio.eu)

On the other hand, the droplet is pulled by driving forces from two opposite directions in the conventional splitting process. (asme.org)
Analyzing the motion by analytical models can be inaccurate as they assume that the droplet retains its circular shape. (asme.org)
Therefore, CFD simulations can demonstrate the droplet behavior better than the analytical models where the droplet exhibits severe deformation and deviation from the circular shape. (asme.org)

Formulation scientists use a core set of analytical techniques to quantify the colloidal, chemical and conformational stability parameters that define the stability of a biotherapeutic. (wikipedia.org)

The traditional bonding techniques used to manufacture silica-particle-based HPLC stationary phases provide many benefits, but can emerging technologies tackle unmet needs? (chromatographyonline.com)
nevertheless, conventional particle sizes are still beneficial due to their higher surface area and their high loadability required for preparative scale applications. (chromatographyonline.com)
Viscoelastic Particle Train Formation in Microfluidic Flows Using a Xanthan Gum Aqueous Solution. (swansea.ac.uk)

The simulations were performed by FLOW-3D software which uses the volume of fluid (VOF) technique. (asme.org)
These techniques are based on the effect of electric fields on molecules suspended in a fluid, and the corresponding induced motion, which can be tuned according to some known physical laws and observed behaviours. (ibecbarcelona.eu)
The developed technique is, thus, well suited for both process monitoring of channel fabrication as well as for studying complex phenomena of fluid flow through nanochannel structures. (mit.edu)
Francesco is interested in teaching topics related to fluid mechanics and rheology, with particular emphasis on the microfluidic-related applications. (swansea.ac.uk)

Advances in microfluidic technology towards flexibility, transparency, functionality, wearability, scale reduction or complexity enhancement are currently limited by choices in materials and assembly methods. (nature.com)
This μFISH implementation makes use of a non-contact scanning probe technology, namely, a microfluidic probe (MFP) that hydrodynamically shapes nanoliter volumes of liquid on a surface with micrometer resolution. (springer.com)
The technology is considered a standard technique among all the technologies. (grandviewresearch.com)

Incorporating imaging capabilities into conventional flow cytometry is highly recommended since cellular morphology analysis is crucial for many biological studies and clinical diagnoses, including cancer screening. (e-consystems.com)
These capabilities make microfluidic modulation spectroscopy a powerful tool in the analysis and development of biosimilars. (wikipedia.org)
The true value of DEP lies in its analytical capabilities which can be achieved by utilizing isomotive dielectrophoresis (isoDEP). (louisville.edu)

nM was achieved using this highly selective PEDOT-based genosensor, without the need for labeling techniques or microelectrode fabrication processes. (ibecbarcelona.eu)

CD, like conventional FTIR, also has major drawbacks. (wikipedia.org)

This method is compatible with the standard workflow of conventional FISH, allows re-budgeting of the sample for various tests, and results in a ~ 15-fold reduction in probe consumption. (springer.com)
As analytical sample management and throughputs increase, we're developing solutions in response to critical laboratory workflow issues," said Jas Sanghera, commercial director at TTP LabTech . (genengnews.com)

The main advantages of using nanofilters, as opposed to conventional systems, are that less pressure is required to pass water across the filter, they are more efficient, and they have incredibly large surface areas and can be more easily cleaned by back-flushing compared with conventional methods," the team says. (nanowerk.com)

On the other hand, this technique can be also used in order to make biosensors reusable, or reconfigurable, by simply inverting its working principle and pulling DNA strands away from the electrodes. (ibecbarcelona.eu)

Based on this we have developed a microfluidic platform that enables isolation of extra cellular vesicles (EVs) from biofluids such a urine, blood plasma and cell culture medium without the need for ultracentrifugation. (selectbiosciences.com)

8 ]. Current research focuses on the optimization of this system as versatile, fast, and sensitive microfluidic POC one-step-immunoassay platform. (hindawi.com)
In this work, we demonstrate the application of the developed microfluidic platform to extract radioactive copper (Cu-64) from an aqueous solution into a solution of a chelating agent in an organic solvent ( e.g. , 2-Hydroxy-4- n -octyloxybenzophenone Oxime in toluene) followed by back extraction into the aqueous phase [7]. (aiche.org)

We systematically optimized several factors affecting the interface stability with the aid of an analytical model, including geometry of the micro-channel, surface functionalization, and the flow rates of the two immiscible streams as well as predicted extraction efficiencies as a function of different paramters. (aiche.org)

Regarding experimental work, learning micro- and nanofabrication methods, performing robotic liquid handling and running various analytical and lab-scale downstream process operation units. (codobio.eu)

A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)

The microfluidic chip is fabricated on standard Si wafers through a CMOS-compatible photolithographic process, allowing for future microelectronics integration. (polito.it)

Electrokinetic techniques are contact-free methods currently used in many applications, where precise handling of biological entities, such as cells, bacteria or nucleic acids, is needed. (ibecbarcelona.eu)
The time required for pathogen analysis is much shorter using molecular methods as compared to conventional culture-based methods. (grandviewresearch.com)
These factors can be attributed to the shifting paradigm of safety testing from conventional cultureâ€ based methods to molecular technologies. (grandviewresearch.com)

Lab2Lab, an automated sample transport system, sends individual microtubes between analytical workstations or laboratories. (genengnews.com)

We are also implementing machine learning within microfluidic applications. (swansea.ac.uk)

The ELISA technique has become a popular standard technique through the microplate format, from R&D to QC and Diagnostics. (interchim.com)

Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique for the binding of the capture protein streptavidin onto planar SiO 2 surfaces such as REA biochips. (hindawi.com)

Also, while a conventional flow cytometer helps in multi-parametric analysis, it lacks spatial resolution. (e-consystems.com)
Fourier-transform infrared spectroscopy (FTIR) secondary structure deconvolution is also used for multivariate analysis techniques including singular value decomposition, partial least squares, soft independent modeling of class analogy, and neural networks. (wikipedia.org)
In 1980's thermal lens spectrometry (TLS) was still considered as a "spectrometric technique on the horizon" as one can also read from one of the textbooks on spectrochemical analysis of that time. (ung.si)

Microfluidic modulation spectroscopy's sensitivity and accuracy, detects higher order structure change in the formulation and at the concentration of interest, without the need for dilution or deuteration. (wikipedia.org)

In analytical similarity studies, the higher order structure of the innovator product is compared to the biosimilar to establish similarity in the structures. (wikipedia.org)
It is one of the only techniques which can directly monitor the formation of aggregates due to its ability to measure intermolecular beta sheet structures. (wikipedia.org)
Microfluidic formation of crystal-like structures. (swansea.ac.uk)

Simultaneous measurement of rheological properties in a microfluidic rheometer. (swansea.ac.uk)

The technique provides information on which structural motifs in the protein molecule are changing, providing more guidance when developing stable protein molecules and formulations. (wikipedia.org)
Comparability and biosimilarity studies often use microfluidic modulation spectroscopy to assess the products for structural differences. (wikipedia.org)
Microfluidic modulation spectroscopy can measure previously undetectable changes in protein structural attributes, changes that are critical to drug efficacy and quality. (wikipedia.org)

For commercially available and published LAMP-based assays, the main analytical characteristics of the tests are listed, which are often comparable to those based on the method of reverse transcription polymerase chain reaction (RT-PCR), and in some cases are even superior. (crie.ru)

By confining FISH probes at the tip of this microfabricated scanning probe, we locally exposed approximately 1000 selected MCF-7 cells of a monolayer to perform incubation of probes - the rate-limiting step in conventional FISH. (springer.com)

As a result, the overlap of the dissolved specimen and the intense light field in the micronsized core is increased manyfold compared to conventional bioanalytical techniques, facilitating highly-efficient photoactivation processes. (mpg.de)

Research Interests: Ultrasonic measurement techniques non-contact ultrasonics (particularly EMATs and laser based ultrasound), non-destructive evaluation and testing, ultrasonic transduction, ultrasonic measurement of metallurgical microstructure, ultrasonic measurement of flow and outside of ultrasonics has interests in eddy current testing and thermography. (gla.ac.uk)

In the second phase of this study, as part of my secondment at UCL, we implemented the robotic liquid handling system to assess the possibility of replacing current conventional resins with multimodal resins. (codobio.eu)

Utilizing this technique, it was doable to watch the presence of mispaired bsAb species and detect and establish further post-translational modifications (PTMs). (humgen.org)

In the study, "saliva was found to be [a more] appropriate sample for CMV than the conventional dried blood spot[s]" that are currently collected from newborns to screen for a number of diseases, Pamula explained. (genomeweb.com)
Existing techniques have been restricted to optical microscopy, which suffers from insufficient resolution, or scanning electron microscopy, which cannot measure sealed or embedded channels without cleaving the sample. (mit.edu)

Circular dichroism spectroscopy (CD) is a technique for the characterization of secondary structure. (wikipedia.org)
CD and conventional FTIR also lack sensitivity in the characterization of biopharmaceuticals proteins such as immunoglobulins IgG1 and IgG2. (wikipedia.org)
Microfluidic modulation spectroscopy is an automated technique that overcomes these challenges of both FTIR and CD for use in biopharmaceutical product characterization. (wikipedia.org)

Microfluidic modulation spectroscopy (MMS) is an infrared spectroscopy technique that is used to characterize the secondary structure of proteins. (wikipedia.org)

As Scott Grant, senior director of programs at My Green Lab, says, "There is no single answer that works for all labs so each must review the techniques that they can implement. (labmanager.com)
lt;span lang="EN-US">This review presents the basic principles of application of the loop-mediated isothermal amplification (LAMP) reaction for the rapid diagnosis of coronavirus infection caused by SARS-CoV-2. (crie.ru)

Thus, the convergence of several factors has made FISH a standard cytogenetic technique for nuclear studies in diagnostics and research (on average, 2.73 FISH papers were published per day in the past 20 years Footnote 1 ). (springer.com)

Thermal lens spectrometry - still a technique on the horizon? (ung.si)

The technique reveals very small conformational differences between different proteins, and provides information on where those differences occur. (wikipedia.org)

In either case, the upper surface of the channel is left open and must be sealed to become an enclosed microfluidic channel (Fig. 1a ). (nature.com)
Our technique is capable of measuring channel dimensions with better than 5-nm accuracy and assessing channel filling within seconds. (mit.edu)

This paper presents a relative slope-boosting technique for a capacitive sensor circuit based on a relaxation oscillator. (sigda.org)

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