AntibodiesDENVSuspected Zika virusInfection with the dengue virusEndemicVACCINEZikaDonated convalescent plasmaChikungunya virusSerotypes of dengueRisk of dengueSevere2023AedesPRNTMosquito-borneViral2020InhibitionFeverSpecimenHemorrhagicAssayPhase serumTitersELISALaboratoryPlasma2022InfectionsSymptomsSerotypeDonorPandemicPatientsClinicalConfirmatoryRespiratoryEncephalitisImmuneOutbreaksLaboratoriesPrimarilySeroconversionVectorAmerican Health OrganizationShock syndromeFlavivirusINTERCEPTVaccinationDetectionZIKVRashAmplificationOccursReplication
Antibodies34
- Serologic testing was negative for Zika virus immunoglobulin M (IgM) antibodies using the CDC Zika IgM antibody capture enzyme-linked immunosorbent assay (Zika MAC-ELISA) and negative for dengue virus and chikungunya virus IgM antibodies using InBios ELISA kits (InBios International, Inc., Seattle, Washington). (cdc.gov)
- Confirmatory serologic testing at the CDC Arbovirus Diagnostic Laboratory was equivocal for Zika virus IgM antibodies using the Zika MAC-ELISA. (cdc.gov)
- Convalescent serologic testing performed at the Maryland DHMH Laboratories Administration on day 56 (40 days after symptom onset) was equivocal for Zika virus IgM antibodies using the CDC Zika MAC-ELISA and negative for dengue virus and chikungunya virus IgM antibodies using InBios ELISA kits. (cdc.gov)
- Serologic testing was positive for Zika virus IgM antibodies using the CDC Zika MAC-ELISA and positive for dengue virus IgM antibodies using an InBios ELISA kit. (cdc.gov)
- Virus-specific IgM antibodies demonstrated in CSF. (cdc.gov)
- Dengue-specific IgM antibodies present in serum with a P/N ratio ≥2. (cdc.gov)
- Dengue viruses are members of the Flaviviridae and have sufficient antigenic similarity to yellow fever virus, Japanese encephalitis virus, and West Nile virus that previous infection or vaccination may raise cross-reactive serum antibodies. (cdc.gov)
- PRNT can often resolve cross-reactive serum antibodies in this situation and identify the infecting virus. (cdc.gov)
- Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted. (who.int)
- Serological detection of total antibodies (IgM and IgG) directed towards Japanese Encephalitis (JE) virus by Hemagglutination Inhibition (HI) assay. (canada.ca)
- Chunpeng Yang , Rui Gong , Natalia de Val , 2019: Development of Neutralizing Antibodies against Zika Virus Based on Its Envelope Protein Structure, Virologica Sinica, 34, 168-174. (virosin.org)
- 11 It is also possible that prior immunisation with yellow fever virus or JEV vaccine may induce cross-reactive antibodies. (mja.com.au)
- 38 Neutralisation tests or an epitope-blocking ELISA can help identify MVEV-specific antibodies. (mja.com.au)
- Further work is aimed at identifying novel monoclonal antibodies and T cell responses to the virus. (uvm.edu)
- 3) Defining B cell memory to Dengue and Zika and developing potent neutralizing antibodies (CDC and NIH, Collaboration with A. De Silva, University of North Carolina, Chapel Hill). (uvm.edu)
- Laboratory testing with a rapid conducted at that time showed that 6.1% of the residents assay suggested that a dengue virus (DENV) was the caus- in nearby regions of Uganda had specifi c antibodies to ative agent. (folkhalsomyndigheten.se)
- Developed by scientists at the New York State Department of Health Wadsworth Center and the University of Texas Medical Branch, the assay outperforms single antigen/antibody ZIKV IgM-Capture ELISA testing in overall sensitivity, specificity, positive predictive value and negative predictive value by detecting IgG-IgA-IgM antibodies to Zika Virus (ZIKV) and Dengue virus (DENV) serotypes 1-4. (healthresearch.org)
- An extra step in our assay measures IgG antibodies by avidity testing, which determines the time-frame of infection, whether primary or secondary, and is quick and simple. (healthresearch.org)
- In ADE, antibodies from a first dengue infection fail to neutralize a second dengue virus of a different serotype during re-infection. (tanzirislambritto.com)
- When a person is infected with the dengue virus for the first time, the immune system responds by producing antibodies specific to that serotype. (tanzirislambritto.com)
- These antibodies will neutralize the virus and aid in its clearance, thus resolving the infection. (tanzirislambritto.com)
- For instance, infants born to dengue-immune mothers are at a higher risk of severe disease due to the presence of maternal antibodies, which can enhance viral infection. (tanzirislambritto.com)
- In the first phase test of the two vaccines, high titers of neutralizing antibodies can be induced, and strong CD4+ and CD8+ reactions are produced, accompanied by mild to moderate adverse reactions. (medicaltrend.org)
- Specific immunoglobulin M (IgM) and neutralizing antibodies to the virus in serum or cerebrospinal fluid (CSF) using enzyme-linked immunosorbent assay (ELISA) technique: IgM is usually but not always present during acute illness. (medscape.com)
- A licensed indirect fluorescent antibody test is available for IgG and IgM antibodies to La Crosse virus and may be useful in diagnosis. (medscape.com)
- A combination of potently neutralizing monoclonal antibodies isolated from an Indian convalescent donor protects against the SARS-CoV-2 delta variant. (thsti.res.in)
- Conclusions Very few healthcare providers had IgG antibodies to SARS-CoV-2, though a significant proportion of them had been infected with the virus. (researchgate.net)
- After SARS-CoV-2 infection, people are unlikely to produce long-lasting protective antibodies against this virus. (researchgate.net)
- As demonstrated in Table 3, there was no significant correlation between days of symptom onset to collection of serum and antibody index/ratio to suggest waning of antibodies during the time period tested. (researchgate.net)
- This information is in contrast to a study in Wuhan, China that found 10% of patients lost SARS-CoV-2 antibodies within weeks of infection (12) , although different serology tests were used in this case. (researchgate.net)
- TWiV 1045: Less Lassa, CRISPR RNA viruses September 17, 2023 TWiV reviews a Lassa virus mRNA vaccine that confers protection against disease without inducing neutralizing antibodies, and a CRISPR-based method for engineering the genome of RNA viruses. (microbe.tv)
- They talk about the upcoming clinical trials of monoclonal antibodies for treatment of COVID-19, and how this method differs from convalescent plasma and vaccination. (justhumanproductions.org)
- Due to this fact, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently wanted for COVID-19 serodiagnosis, convalescent plasma remedy, and vaccine growth. (genediag.com)
- The performance of the nanoribbon biosensor upon the use of two different types of molecular probes - the antibodies and the aptamers against HCVcoreAg - has been tested. (preprints.org)
DENV5
- The four dengue serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) circulate throughout the Americas, and in some countries simultaneously. (paho.org)
- Dengue is a mosquito-borne viral infection associated with significant morbidity and mortality caused by any of four closely related virus serotypes (DENV-1,-2,-3 and -4), all of which circulate in the World Health Organization (WHO) Western Pacific Region. (who.int)
- 4) Transcriptional profiling of DENV virus infection (Joint College of Medicine/Engineering Biomedical Engineering grant with Donna Rizzo and John Hanley from UVM and Sam Scarpino from Northeastern). (uvm.edu)
- While several lines of evidence suggest a protective role of T cells against disease associated with Dengue virus (DENV) infection, their potential contribution to immunopathology in the acute phase of DENV infection remains controversial, and it has been hypothesized that the more severe form of the disease (dengue hemorrhagic fever, DHF) is associated with altered T cell responses. (mdpi.com)
- Since ZIKV, DENV and other flaviviruses including chikungunya, an arbovirus, co-circulate in the same regions, a positive result for one of these viruses does not preclude infection with the others. (healthresearch.org)
Suspected Zika virus1
- the provider suspected Zika virus infection, and serum and urine specimens were collected. (cdc.gov)
Infection with the dengue virus2
- Dengue hemorrhagic fever, which has also been called Philippine, Thai, or Southeast Asian hemorrhagic fever, frequently requires prior infection with the dengue virus. (msdmanuals.com)
- Interestingly, it's not the first infection with the dengue virus that usually causes DHF but rather a subsequent infection with a different dengue serotype. (tanzirislambritto.com)
Endemic6
- The second reason dengue should concern health care providers in this country is the potential for encountering cases of dengue among travelers arriving from dengue-endemic areas. (cdc.gov)
- all the rest were travelers arriving from dengue-endemic areas. (cdc.gov)
- Dengue hemorrhagic fever is a variant presentation of dengue infection that occurs primarily in children 10 years living in areas where dengue is endemic. (msdmanuals.com)
- Dengvaxia is approved for use in individuals 9 through 16 years of age with laboratory-confirmed previous dengue infection and living in endemic areas. (cdc.gov)
- Suspected infection with Japanese Encephalitis virus and has travel to endemic area. (canada.ca)
- The mosquito-borne flaviviruses dengue and zika cause endemic or pandemic disease and long-term sequelae across over 40% of the world's population and there are no broadly licensed vaccines for either. (uvm.edu)
VACCINE9
- The Omicron S-bearing virus robustly escapes vaccine-induced humoral immunity, mainly due to mutations in the receptor-binding motif (RBM), yet unlike naturally occurring Omicron, efficiently replicates in cell lines and primary-like distal lung cells. (biorxiv.org)
- This knowledge is critical to developing better vaccines and to develop better laboratory tests to determine whether vaccine candidates engage the immune system in a way that is likely to lead to protection. (uvm.edu)
- Our work on dengue and zika immunology draws on an extensive bank of human vaccine clinical trial specimens and natural infection studies in partnership with the Vaccine Testing Center and other collaborators. (uvm.edu)
- More recently we seek to understand how norovirus, a family of viruses for which there is no vaccine and causes significant gastrointestinal illness (vomiting and diarrhea) and hospitalization can skew the infant antibody response towards those that may not offer protection. (uvm.edu)
- 1) Defining cellular immunogenicity for dengue vaccine formulations (Bill and Melinda Gates Foundation, NIH). (uvm.edu)
- Most SARS-CoV-2 vaccine candidates produce an immune response to the spike protein on the surface of the virus. (medicaltrend.org)
- Cellular Immune Responses are Preserved and may Contribute to Vaccine Effectiveness Despite Reduced Virus Neutralization Against Infection due to SARS-CoV-2 B.1.617.2 Variant. (thsti.res.in)
- Classic examples of VAED are atypical measles and enhanced respiratory syncytial virus (RSV) occurring after administration of inactivated vaccine for these pathogens. (cov19longhaulfoundation.org)
- A high-throughput neutralizing antibody assay for COVID-19 analysis and vaccine analysis Virus neutralization stays the gold normal for figuring out antibody efficacy. (genediag.com)
Zika11
- Laboratory testing at the Maryland DHMH Laboratories Administration confirmed Zika virus infection. (cdc.gov)
- Case investigation revealed that the woman had not traveled to a region with ongoing transmission of Zika virus, but did have sexual contact with a male partner who had recently traveled to the Dominican Republic. (cdc.gov)
- The male partner reported exposure to mosquitoes while traveling, but no symptoms consistent with Zika virus infection either before or after returning to the United States. (cdc.gov)
- On day 16 (2 and 6 days after the episodes of condomless vaginal intercourse) the woman developed symptoms of Zika virus infection, including fever and rash. (cdc.gov)
- Zika virus RNA was detected in urine, but not in serum, by real-time reverse transcription-polymerase chain reaction (rRT-PCR) using a test based on an assay developed at CDC ( 1 ). (cdc.gov)
- Plaque-reduction neutralization tests (PRNTs) performed at the CDC Arbovirus Diagnostic Laboratory confirmed a recent Zika virus infection. (cdc.gov)
- Zika virus rRT-PCR testing performed at the Maryland DHMH Laboratories Administration was negative on serum and plasma and equivocal on urine. (cdc.gov)
- Semen collected on day 31 had no detectable Zika virus RNA by rRT-PCR testing performed at the Maryland DHMH Laboratories Administration. (cdc.gov)
- 181B potentially associated with Zika virus (ZIKV) has highlighted the urgent need to identify individuals infected with ZIKV. (who.int)
- Dr. Diehl's work in the Department of Microbiology and Molecular Genetics encompasses translational and basic research aimed at understanding the human immune response to viral infections, primarily to dengue virus, zika virus, norovirus, and SARS-CoV-2. (uvm.edu)
- Zika virus (ZIKV) is a mosquito-borne fl avivirus fi rst studies in the absence of epidemics ( 6 - 8 ). (folkhalsomyndigheten.se)
Donated convalescent plasma1
- On August 23, 2020, Cerus received US FDA Emergency Use Authorization to treat the donated convalescent plasma from recovered COVID-19 patients, with the purpose of giving investigative antibody immunotherapy transfusions to other COVID-19 patients suffering an infection. (wikipedia.org)
Chikungunya virus3
- Flavivirus and chikungunya virus tests were performed at the Maryland DHMH Laboratories Administration. (cdc.gov)
- Serum rRT-PCR testing for dengue virus and chikungunya virus was negative. (cdc.gov)
- Dengue-like epidemics occurred in Egypt and on Java in 1779, but these may actually have been caused by chikungunya virus. (cdc.gov)
Serotypes of dengue2
- Dengue and dengue hemorrhagic fever (DHF) result from infection by any of four serotypes of dengue viruses. (cdc.gov)
- It is transmitted by the bite of a mosquito infected with one of the four serotypes of dengue virus. (paho.org)
Risk of dengue2
Severe14
- 1) Outbreaks of the more severe form of dengue, DHF, occurred in Cuba in 1981 (2) and in Venezuela in 1989. (cdc.gov)
- Although the incidence of hemorrhagic dengue in United States travelers abroad is low,(13,15) clinicians in this country may encounter severe cases of dengue, particularly among immigrants from areas with a high incidence of DHF. (cdc.gov)
- 14,18,19) Nevertheless through the first half of the 20th century, dengue was generally described as a self-limited, nonfatal febrile illness, with occasional hemorrhagic manifestations such as petechiae, epistaxis, gingival bleeding and menorrhagia, that only rarely resulted in more severe or fatal outcomes. (cdc.gov)
- The illness can evolve to severe dengue, characterized by shock, respiratory distress, severe bleeding, and/or serious organ impairment. (paho.org)
- Following infection with one serotype, subsequent infection with a different serotype increases a person's risk of severe dengue and death. (paho.org)
- The disease can evolve into severe dengue, characterized by shock, shortness of breath, severe bleeding and / or complications in the organs. (paho.org)
- d WHO Collaborating Centre for Arbovirus Reference and Research (Dengue/Severe Dengue), Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. (who.int)
- The majority (~75%) of infections in humans are asymptomatic, but a small proportion of symptomatic patients develops severe dengue characterized by rapid progression into shock, severe bleeding and/or multiorgan impairment, which leads to death if unattended or mismanaged. (who.int)
- It causes high fever and the typical flu-like symptoms seen in dengue and leads to severe bleeding, blood plasma leakage, and low platelet count. (tanzirislambritto.com)
- Instead, they aid the virus entering host cells, enhancing its reproduction ability and leading to severe disease. (tanzirislambritto.com)
- This leads to increased viral replication, a higher viral load, and a much more severe disease, resulting in DHF or even more severe dengue shock syndrome (DSS). (tanzirislambritto.com)
- Additionally, this section includes updated remarks for the existing recommendation on the use of convalescent plasma for ambulatory patients with mild-to-moderate COVID-19 at high risk for progression to severe disease who have no other treatment options. (idsociety.org)
- HAI antibody responses were significantly higher in patients who experienced severe disease, including respiratory failure and acute respiratory distress syndrome, compared with patients who experienced less severe illness.ConclusionsPatients with A(H7N9) virus infection who survived severe disease mounted higher antibody responses that persisted for longer periods compared with those that experienced moderate disease. (ox.ac.uk)
- Studies of convalescent plasma treatment for A(H7N9) patients should consider collection of donor plasma from survivors of severe disease between 1 and 11 months after illness onset. (ox.ac.uk)
20233
- TWiV 1047: Long COVID and avian flu in ferrets September 24, 2023 TWiV explains a study of postacute sequelae of COVID-19 at 2 years, and airborne transmission of human-isolated avian H3N8 influenza virus between ferrets. (microbe.tv)
- Matters Microbial #18: The Perfect Predator: Why Phage is Rage November 30, 2023 Today Dr. Steffanie Strathdee, Associate Dean of Global Health Sciences at UC San Diego and Co-Director at the Center for Innovative Phage Applications and Therapeutics, will chat with us about how bacteriophages-viruses that attack bacteria-changed her life and are becoming part of our future. (microbe.tv)
- TWiEVO 95: Watering the viruses until they bloom November 14, 2023 Nels and Vincent discuss how the rewetting of seasonally dried soils, a critical event in Mediterranean grasslands that reactivates dormant soil microorganisms, leading to pulses of carbon and nitrogen mineralization, and is accompanied by a bloom of viral diversity, followed by extensive viral community turnover. (microbe.tv)
Aedes5
- 10) Adding to the concern of indigenous dengue transmission is the recent establishment in the United States of another known dengue vector, Aedes albopictus, which was probably imported in shipments of tires from Asia. (cdc.gov)
- In the Americas, Aedes aegypti is the mosquito vector that is the main source of dengue transmission. (paho.org)
- tory testing to the Arbovirus Diagnostic Laboratory at the Over the next 20 years, several ZIKV isolates were Centers for Disease Control and Prevention (CDC, Fort obtained from Aedes spp. (folkhalsomyndigheten.se)
- The Aedes mosquito, mainly Aedes aegypti and, to a lesser extent, Aedes albopictus, plays a crucial role in transmitting the dengue virus, which causes Dengue Hemorrhagic Fever. (tanzirislambritto.com)
- Overwintering of La Crosse virus in Aedes triseriatus. (medscape.com)
PRNT5
- The plaque reduction neutralization test (PRNT) is specific and sensitive. (msdmanuals.com)
- The PRNT requires live dengue viruses for the test and is labor-intensive and expensive. (msdmanuals.com)
- this population is not likely to qualify for access to confirmatory plaque reduction neutralization testing (PRNT) and therefore the virus could go undetected. (healthresearch.org)
- Confirmatory PRNT is expensive, time-consuming (one-week turn-around), has low sample throughput, and uses live virus in the lab, which creates a biosafety risk. (healthresearch.org)
- Another drawback of PRNT is that it has difficulties distinguishing the infecting virus (primary) in people with, or vaccinated against, a secondary infection. (healthresearch.org)
Mosquito-borne1
- Murray Valley encephalitis virus (MVEV) is a mosquito-borne virus that is found across Australia, Papua New Guinea and Irian Jaya. (mja.com.au)
Viral3
- Dengue virus-specific viral antigen or genomic sequences demonstrated in donated blood or organs during screening and confirmatory testing in the absence of symptoms in the donor. (cdc.gov)
- Travelers were categorized as infectious during travel if they had arrival dates from 2days before to 10days after symptom onset or a positive viral test. (cdc.gov)
- Isolation of an arbovirus, detection of specific antibody by plaque reduction neutralization assay or detection of nucleic acid by real-time RT-PCR in a clinical specimen would constitute firm evidence of viral association with illness and provide 'confirmed case' status. (canada.ca)
20201
- In March 2020 we implemented broad serological testing for SARS-CoV-2, the virus underlying COVID-19 used to profile hospitalized COVID-19 patients as well as those exposed to the virus but did not require hospitalization. (uvm.edu)
Inhibition3
- 29 , 37 Most reference laboratories have developed in-house MVEV serological tests that can detect total antibody by haemagglutination inhibition, IgG by immunofluorescence assays (IFA) or enzyme immunoassays (EIA), and IgM by IFA or EIA. (mja.com.au)
- Significant antibody titers include levels of more than 320 by hemagglutination inhibition, more than 128 by complement fixation, more than 256 by immunofluorescence, or more than 160 by plaque reduction neutralization test. (medscape.com)
- A(H7N9) virus-specific antibody responses were assessed by hemagglutination inhibition (HAI) and neutralization (NT) assays. (ox.ac.uk)
Fever9
- Convalescent treatments using INTERCEPT are also being applied to Ebola survivor plasma donations at Emory University, and platelet donations in Puerto Rico, which has dengue fever and chikungunya infections in their population. (wikipedia.org)
- Dengue hemorrhagic fever often begins with abrupt fever and headache and is initially indistinguishable from classic dengue. (msdmanuals.com)
- or vaginal bleeding) but not meeting the case definition of dengue hemorrhagic fever. (cdc.gov)
- Yellow Fever, Dengue, Powassan, etc.) detection of total anti-JE virus specific antibody in a single serum sample is indicative of past or present exposure to this agent, or a related agent from the same virus genus. (canada.ca)
- 33 Antigen detection assays are not available for MVEV, but have been used for JEV and Dengue fever virus. (mja.com.au)
- Z ported human infections indicated that clinical characteris- ika virus (ZIKV) is a mosquito-transmitted virus in the tics of infection with ZIKV included fever, headache, mal- family Flaviviridae and genus Flavivirus . (folkhalsomyndigheten.se)
- Dengue Hemorrhagic Fever (DHF) is an acute and potentially lethal complication of dengue fever, a tropical disease caused by the dengue virus. (tanzirislambritto.com)
- DHF is marked by the unique clinical features that differentiate it from common dengue fever. (tanzirislambritto.com)
- The primary cause of Dengue Hemorrhagic Fever (DHF) is infection by one of the four dengue virus serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). (tanzirislambritto.com)
Specimen2
- Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, and clinical and travel history of patient. (canada.ca)
- tive for ZIKV in their convalescent-phase specimen. (folkhalsomyndigheten.se)
Hemorrhagic1
Assay1
- This biopsy material tested positively for La Crosse virus antigen on direct immunofluorescence assay. (medscape.com)
Phase serum3
- Acute-phase/convalescent-phase serum samples. (cdc.gov)
- Titers in acute and convalescent phase serum samples can reliably establish dengue virus infection and may indicate the specific dengue virus type involved. (msdmanuals.com)
- A fourfold or greater increase or decrease in titre between the acute phase and convalescent phase serum is considered to be diagnostic of infection with Japanese Encephalitis virus. (canada.ca)
Titers1
- HAI antibody responses were compared by clinical severity.ResultsOf 67 patients with A(H7N9) virus infection, HAI antibody titers reached 40 on average 11 days after illness onset and peaked at a titer of 290 after three months, and average titers of ≥80 and ≥40 were present until 11 months and 22 months respectively. (ox.ac.uk)
ELISA1
- After a primary infection with a heterologous flavivirus, subsequent antibody testing by ELISA may produce false positive results for a different flavivirus. (cdc.gov)
Laboratory2
Plasma9
- The UV exposure process works by intentionally damaging DNA and RNA material in foreign virus and bacteria that might be present, while remaining harmless to the donated blood plasma and platelets (which contains no RNA or DNA). (wikipedia.org)
- In addition to pathogen neutralization, there are clinical trials in West Africa to test the effectiveness of using the INTERCEPT system to treat convalescent plasma from Ebola survivors, and administering the treated plasma to other patients still fighting an infection. (wikipedia.org)
- Similarly, the US FDA has granted emergency use of INTERCEPT to sterilize convalescent COVID-19 patient plasma, although the US medical community harbors some disagreements on whether this approach will provide effective relief for its patients. (wikipedia.org)
- In developing countries with lower levels of health care access, the product is finding greater clinical and humanitarian trial opportunities due to high rates of collecting and using unsafe donated whole blood, low access to plasma and platelet separating equipment, and low supply of screening tests. (wikipedia.org)
- A new recommendation was developed against the routine use of convalescent plasma among immunocompromised patients hospitalized with COVID-19. (idsociety.org)
- He worked with Dr. Emil Behring, the Nobel Prize-winning scientist who developed convalescent plasma: the treatment we talked about last Tuesday in our series of episodes on immunology. (justhumanproductions.org)
- Remember, convalescent plasma or serum, is when you take blood from someone immune to a virus or bacteria, and give their antibody-rich blood to another patient to help them fight off the disease. (justhumanproductions.org)
- See, after Erhlich helped Behring figure out convalescent plasma… he wanted to go a step further. (justhumanproductions.org)
- We isolated an N417-dependent antibody, 084-7D, which showed similar neutralization breadth to the plasma. (bvsalud.org)
20221
- Of 22,079 participants tested between 2016 and 2022, 96 had positive saliva results, 15 were determined to be FP, 79 TP, and 2 were excluded for incomplete clinical evaluation. (cdc.gov)
Infections1
- The attempts to treat virus infections occur even earlier than there was an understanding of the concept of a virus as an independent entity. (medsci.org)
Symptoms2
- 28 Indeed, by the time neurological symptoms develop, PCR testing of serum or CSF often returns a negative result. (mja.com.au)
- Given that these enhanced responses are triggered by failed attempts to control the infecting virus, VAED typically presents with symptoms related to the target organ of the infection pathogen. (cov19longhaulfoundation.org)
Serotype3
- All 16 laboratories testing Module A performed reverse transcriptase polymerase chain reaction (RT-PCR) for both RNA and serotype detection. (who.int)
- However, problems arise when the person is later infected with a different dengue serotype. (tanzirislambritto.com)
- DHF and DSS are also influenced by various other factors, such as the strain and serotype of the dengue virus, age, genetic predisposition, and the individual's immune status. (tanzirislambritto.com)
Donor1
- Due to this possible association, screening programs that undertake testing saliva for CMV DNA may benefit from documenting donor milk use as a potential increased risk for FP results. (cdc.gov)
Pandemic2
- The order in which the 20 questions are discussed is based on how a pandemic would usually be analyzed by public health physicians when they use an objective, strictly scientific problem-solving approach-starting with a fundamental first question "how threatening is the virus? (notesfromthesocialclinic.org)
- At the beginning of the COVID pandemic, it was feared that the SARS-CoV-2 virus might represent the most threatening respiratory virus since the deadly 1918 Spanish influenza virus, which was an H1N1 influenza virus of avian origin. (notesfromthesocialclinic.org)
Patients4
- Patients with World Health Organization-defined clinical criteria plus thrombocytopenia ( ≤ 100,000/mcL [≤ 100 x 10 9 /L]) or hemoconcentration (Hct increased by ≥ 20%) are presumed to have the disease (see the Centers for Disease Control and Prevention's Dengue Virus: Clinical Guidance ). (msdmanuals.com)
- A subset of ZIKV-infected insuffi cient to confi rm ZIKV as the recently infecting fl a- patients for whom acute- and convalescent-phase paired vivirus. (folkhalsomyndigheten.se)
- We tested 49 known coronavirus disease-19 (COVID-19) patients and 111 prepandemic stored serology specimens. (researchgate.net)
- ObjectivesThe long-term dynamics of antibody responses in patients with influenza A(H7N9) virus infection are not well understood.MethodsWe conducted a longitudinal serological follow-up study in patients who were hospitalized with A(H7N9) virus infection, during 2013-2018. (ox.ac.uk)
Clinical6
- The early history of dengue is clouded by the similarity of its clinical picture to that of other febrile illnesses. (cdc.gov)
- A universal screening research study was conducted in six hospitals to identify the clinical sensitivity of polymerase chain reaction (PCR) testing on newborn dried blood spots (DBSs) versus saliva specimens for the diagnosis of congenital cytomegalovirus (cCMV). (cdc.gov)
- 1,2 Dengue presentation is broad and non-specific, which may confound clinical diagnosis. (who.int)
- Mpox virus is known to be transmissible from the onset of clinical manifestations. (bvsalud.org)
- The diagnosis of California encephalitis is based on immunology, because the virus is not present in blood or secretions during clinical CNS disease. (medscape.com)
- Moderna have been claiming that neutralization activity against the more immune-evasive variants has fallen below the protection level at the 6-8 month point in the clinical trial subjects, so seeing a lot of breakthroughs in Israel and at this time makes sense if they were indeed correct. (nakedcapitalism.com)
Confirmatory1
- Serological tests remain the mainstay of confirmatory diagnostic testing. (mja.com.au)
Respiratory3
- This work also directly led to the development of a new antibody (Nirsevimab) that can protect premature infants after one dose for an entire winter season against respiratory syncytial virus (RSV). (uvm.edu)
- The potential for vaccination against SARS-CoV-2 to be associated with disease enhancement is of theoretical concern, given similar observations with other respiratory viruses in general, and in animal models of highly pathogenic coronaviruses in particular [14] . (cov19longhaulfoundation.org)
- On average, people infected with the delta variant had about 1,000 times more copies of the virus in their respiratory tracts than those infected with the original strain of the coronavirus, the study reported. (nakedcapitalism.com)
Encephalitis3
- Ribavirin (already established as therapy for hepatitis C virus, which is also in the Flaviviridae family) was used in a study of 153 Indian children with JEV encephalitis, but did not indicate any benefit compared with placebo. (mja.com.au)
- Polymerase chain reaction (PCR) for the diagnosis of La Crosse encephalitis and Metagenomic next-generation sequencing (NGS) of the CSF has the potential to identify a broad range of pathogens in a single test (ref DOI: 10.1056/NEJMoa1803396). (medscape.com)
- California encephalitis virus, a newly described agent. (medscape.com)
Immune4
- dengue virus-antibody immune complexes trigger release of vasoactive mediators by macrophages. (msdmanuals.com)
- Instead, they form complexes with the virus that are more readily taken up by cells involved in the immune response. (tanzirislambritto.com)
- To sum up, the cause of DHF is multi-factorial, involving not only the dengue virus and its serotypes but also the human immune response, the vector mosquito species, and various ecological factors. (tanzirislambritto.com)
- The immune system could play a crucial role in virus infection. (medsci.org)
Outbreaks3
- 9) Outbreaks of indigenous dengue transmission occurred in Texas in 1980 and again in 1986. (cdc.gov)
- In the Western Pacific Region, dengue outbreaks occur yearly in multiple countries, driven by a complex interplay of virus, vector and host biology, climatic and socioeconomic factors as well as international travel and trade. (who.int)
- In 2013, outbreaks resulted in 44 098 dengue cases in the Lao People's Democratic Republic, 39 222 cases in Malaysia, 10 548 cases in New Caledonia and 22 170 cases in Singapore. (who.int)
Laboratories5
- In 2008, PAHO / WHO Member States established a Network of Dengue Laboratories of the Americas (RELDA), to strengthen technical capacities for diagnosing dengue. (paho.org)
- The objective of this programme was to assess dengue diagnostic proficiency among national-level public health laboratories in the World Health Organization (WHO) Western Pacific Region. (who.int)
- Detection of ongoing/recent dengue virus infection by both molecular (RT-PCR) and serological methods (IgM) was available in 15/19 participating laboratories. (who.int)
- This first round of external quality assessment of dengue diagnostics was successfully conducted in national-level public health laboratories in the WHO Western Pacific Region, revealing good proficiency in both molecular and serological testing. (who.int)
- Only a few state laboratories or other specialized laboratories, including those at the Centers for Disease Control and Prevention (CDC), are capable of performing this specialized testing. (medscape.com)
Primarily1
Seroconversion1
- To demonstrate seroconversion, both acute and convalescent samples must be tested in parallel. (canada.ca)
Vector5
- Dengue Branch and the Division of Vector Borne Infectious Diseases, Centers for Disease Control, CID, Fort Collins, CO. Duane J. Gubler, Sc.D and Edward B. Hayes, MD. (cdc.gov)
- is the vector mosquito for dengue and is widely distributed in the Americas. (paho.org)
- Only Canada and continental Chile are free from dengue and its vector. (paho.org)
- These mosquitoes are highly adaptive to the human environment, coupled with increasing urbanization and inadequate vector control, which has led to a surge in dengue transmission in urban and semi-urban settings. (tanzirislambritto.com)
- Two different HCVcoreAg preparations have been tested: recombinant b-galactosidase-conjugated HCVcoreAg ("Virogen", USA) and recombinant HCVcoreAg ("Vector-Best", Russia). (preprints.org)
American Health Organization1
Shock syndrome1
- Circulatory collapse and multiorgan failure, called dengue shock syndrome, may develop rapidly 2 to 6 days after onset. (msdmanuals.com)
Flavivirus1
- These viruses are members of the Flavivirus genus. (tanzirislambritto.com)
INTERCEPT1
- The Helinx technology and INTERCEPT system have been shown effective on several types of viruses, but it is not universally effective due to some kinds of pathogens' RNA or DNA being more resilient to the process (such as hepatitis A, human parvovirus 19 and some bacterial spores). (wikipedia.org)
Vaccination1
- At the other end of the spectrum has been the view that SARS-CoV-2 is so threatening that it absolutely necessitates acceptance of the risks associated with mass vaccination with suboptimal and inadequately studied vaccines, as well as the risks associated with prolonged lockdown measures, until 100% of the world's population (including children) has been fully vaccinated (i.e., "zero tolerance," complete eradication of the virus). (notesfromthesocialclinic.org)
Detection3
- Of these, 15 had correct results for RNA detection and all 16 correctly serotyped the viruses. (who.int)
- In addition, antibody detection may provide a complementary perspective, along with RT-qPCR testing, in the diagnosis of COVID-19 (24,25). (researchgate.net)
- Detection of Mpox Virus Using Microbial Cell-free DNA: the Potential of Pathogen-Agnostic Sequencing for Rapid Identification of Emerging Pathogens. (amedeo.com)
ZIKV1
- Since the initial outbreak of the disease, it has been recognized that the sensitivities and specificities for current ZIKV testing algorithms have shortcomings. (healthresearch.org)
Rash1
- 5) Cellular and molecular characterization of dengue-associated rash (Merck). (uvm.edu)
Amplification2
- Combined with the dengue virus RNA amplification test, it can provide a diagnosis within the first 1 to 7 days of illness. (msdmanuals.com)
- In fatal cases, nucleic acid amplification, histopathology with immunohistochemistry and virus culture of autopsy tissues can also be useful. (medscape.com)
Occurs1
- Moreover, DHF occurs when a person is infected with the dengue virus for the second time. (tanzirislambritto.com)
Replication2
- Replication of Dengue Virus in K562-Megakaryocytes Induces Suppression in the Accumulation of Reactive Oxygen Species. (thsti.res.in)
- Non-structural proteins are involved in the transcription and replication of the virus. (medsci.org)