Control of cell cycle bacterial cells cell cycle. Medical search. Frequent questions

The central aim of the research within the Cell Biology theme is to gain a deeper understanding of the biology of the eukaryotic cell. (ki.se)
20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
Our lab has as its general focus one of the fundamental regulatory systems of eukaryotic cells - the ubiquitin system. (yale.edu)
First, we wish to understand, at a mechanistic and molecular level, how specific proteins are rapidly degraded within eukaryotic cells while most proteins are spared. (yale.edu)
A third, more recent research focus has been on endosymbiotic bacteria that manipulate their eukaryotic hosts by secreting enzymes, including ubiquitin-specific proteases, into the host cell cytoplasm. (yale.edu)
The Type VI secretion system (T6SS) is widely used by bacterial pathogens as an effective weapon against bacterial competitors and is also deployed against host eukaryotic cells in some cases. (dundee.ac.uk)
As a card-carrying cell biologist, Goley was next drawn to the fledgling field of bacterial cell biology and brought her expertise in eukaryotic cytoskeletal biochemistry to studying the cytoskeleton of Caulobacter crescentus as a postdoctoral researcher with Lucy Shapiro at Stanford University. (rupress.org)
We knew, and still know, far less than for eukaryotic cytoskeletons about what these bacterial polymers really do, how their structures relate to their functions, or how they are regulated by interacting partners. (rupress.org)
These bugs have a knack for pinpointing critical cell functions, she says, adding fondly that Legionella is continuing to help her explore how eukaryotic cells work. (the-scientist.com)
CELL DIVISION by mitosis, meiosis and binary fission - How do eukaryotic and prokaryotic cells multiply? (docbrown.info)
Most c-di-GMP-dependent signaling pathways control the ability of bacteria to interact with abiotic surfaces or with other bacterial and eukaryotic cells (Romling et al, 2013). (learnlifescience.com)
2010). LapB, a novel Listeria monocytogenes LPXTG surface adhesin, required for entry into eukaryotic cells and virulence . (up.pt)

She received her PhD in Biochemistry in 1996 from the University of Liège, Belgium where she unraveled a molecular mechanism by which some bacterial pathogens sense and respond to antibiotics attack to achieve resistance. (stanford.edu)
Host-directed antimicrobial drugs with broad-spectrum efficacy against intracellular bacterial pathogens. (harvard.edu)
Matt Welch's purification of Arp2/3 as the host factor that nucleates actin on the surface of Listeria is one of Goley's all-time favorite experiments: "I love the concept that intracellular pathogens are the best cell biologists around and that we can learn so much about fundamental cell biology by discovering how they manipulate it to their advantage. (rupress.org)
The stunning diversity observed in the cell biology of different bacteria, the dangerous rise in antibiotic resistance, and the importance of bacteria to human health both as pathogens and as integral components of our microbiota continue to affirm my original motivation to study fundamental aspects of bacterial cell biology. (rupress.org)
Scientists studying pathogens such as Chlamydia , Legionella , and Listeria get a master class in how to control the internal workings of mammalian cells. (the-scientist.com)
These microbes all enjoy at least part of their lives shielded from the onslaught of white blood cells, antibodies, and other immune defenses that the body launches against pathogens that live outside of host cells. (the-scientist.com)
By then it was known that Listeria infects cells such as macrophages-motile human immune cells that engulf pathogens and cellular debris-by being taken up into vacuoles and breaking out of those vacuoles into the cytoplasm. (the-scientist.com)

McAdams and his collaborators have published foundational insights on the nature of genetic regulatory logic and cell biology, the molecular basis for inevitable random variation levels of protein production between different cells, and genetic logic circuits that control the bacterial cell cycle. (wikipedia.org)
CMB is comprised of more than 30 independent research groups organized in three themes: Cell Biology, Developmental and Stem Cell Biology and Infection and Cancer. (ki.se)
Thus the theme encloses expertise within bioinformatics, biophysics, biochemistry, molecular cell biology and genetics. (ki.se)
The stem cell and developmental biology program at CMB is engaged in a broad range of basic research aimed at understanding the molecular mechanisms underlying diverse aspects of embryonic development and tissue regeneration. (ki.se)
Basic genetic and molecular biology methodologies are combined with complementary bioinformatic and genome-wide approaches, allowing investigation global molecular changes in cells in various differentiation processes. (ki.se)
Our lab is interested in the systems biology and evolution of epigenetic switches (bistability) and clocks (oscillators) in gene regulatory networks, two functions that are essential for patterning, cell proliferation, and differentiation in biological systems. (duke.edu)
Over the years, her group made major contributions in the emerging field of bacterial cell biology and provided key molecular insights into the temporal and spatial mechanisms involved in cell morphogenesis, cell polarization, chromosome segregation and cell cycle control. (stanford.edu)
For her distinguished work, she received the Pew Scholars award from the Pew Charitable Trust, the Woman in Cell Biology Junior award from the American Society of Cell Biology and the Eli Lilly award from the American Society of Microbiology. (stanford.edu)
A s a grad student in cell biology, Shaeri Mukherjee was always on the lookout for new ways to fiddle with cells' internal structures. (the-scientist.com)
For Mukherjee, the paper was a revelation: not only did it identify the fastest way yet to target Golgi biology, it suggested that scientists could use intracellular bacteria "as a lens to understand basic processes inside the cell. (the-scientist.com)
Research on how intracellular bacteria take control of their hosts is not only informing scientists about how these microbes cause disease, but revealing secrets of mammalian biology, says Mukherjee, who now heads up a lab at the University of California (UC), San Francisco. (the-scientist.com)
Nature Cell Biology, 7 (10), 954 - 960. (up.pt)
Current Opinion in Cell Biology, 17 (5 SPEC. (up.pt)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

The key interests lie within the field of genome integrity and variation, cell division, regulation of gene expression, protein turnover and signal transduction. (ki.se)
Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system. (jic.ac.uk)
Our study reveals the subcellular dynamics and multi-layered regulation of the ParABS system, coupled to the cell cycle of B. bacteriovorus. (jic.ac.uk)
During her postdoctoral work at Stanford Medical School, she demonstrated that bacteria can localize regulatory proteins to specific intracellular regions to control signal transduction and the cell cycle, uncovering a new, unsuspected level of bacterial regulation. (stanford.edu)
In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)
Here, we used the anti-bacterial T6SS of Serratia marcescens to elucidate post-translational regulation of offensive T6SS deployment, using single-cell microscopy and genetic analyses. (dundee.ac.uk)
We have found that cell size regulation and timing of chromosome replication are interconnected, a connection that is influenced by nutrient availability. (illinois.edu)
This is interesting because the molecular factors responsible for regulating cell size in bacteria and the mechanism that coordinate cell size regulation with the progression of the cell cycle remain unclear. (illinois.edu)
We are currently working out the molecular network that links cell shape regulation and ability to resist toxic compounds. (illinois.edu)
Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. (molcells.org)
Bacterial QS regulation is established through a wide range of signals such as oligopeptides, N-acyl homoserine lactones, furanose borate, hydroxy palmitic acid methyl ester, and methyl decanoic acid. (learnlifescience.com)

Two critical factors during this dauntingly complex process appear to be the establishment of metabolic connectivity between the symbiotic partners, and the evolution of nuclear control over protein expression levels within the organelle. (frontiersin.org)
Bacterial origins regulate orisome assembly, a nuclei-protein complex assembled on the origin responsible for unwinding the origin and loading all the replication machinery. (wikipedia.org)
In E. coli, the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication (oriC) which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom). (wikipedia.org)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
The carbohydrate composition of a myeloma protein from different subcellular fractions of plasma cells. (uni-mainz.de)
One such protein that we study, SUMO, is attached to many proteins and is crucial for cell-cycle progression. (yale.edu)
We are trying to understand the functional consequences of SUMO-protein modification, particularly in the cell cycle and chromatin-mediate gene transcription, and to determine the basis of specificity for the SUMO-cleaving proteases. (yale.edu)
Defensive' T6SSs assemble and fire in response to incoming attacks from aggressive neighbouring cells, and can utilise the Threonine Protein Phosphorylation (TPP) regulatory pathway to achieve this control. (dundee.ac.uk)
We have identified a novel protein that looks structurally like a DNA repair protein and yet this protein has the capability of making cells resistant to antibiotics that intercalate DNA. (illinois.edu)
The effect of SJZT on the expression of PD-L1 protein in A549 cells was detected by Western Blotting (WB). (bvsalud.org)
The cloning of maize PPDK regulatory protein (PDRP) revealed the regulatory protein had been sequenced from more than 200 bacterial species and had been identified as DUF299 (domain of unknown function). (scirp.org)
Roy's team had found that "this protein called AnkX, when microinjected into cells-even at picomole levels-could cause the entire Golgi to fragment in, like, five minutes," Mukherjee says. (the-scientist.com)
Massive-scale transient expression in Chinese language Hamster Ovary (CHO) cells offers a fast protein manufacturing methodology with a possible start-to-end alignment benefit for biotherapeutics drug discovery. (molvisindex.org)
2012). Listeria monocytogenes triggers the cell surface expression of Gp96 protein and interacts with its N terminus to support cellular infection . (up.pt)
Translocating the CagA protein into the gastric epithelial cells causes rearrangement of the host cytoskeleton and alters cell signaling and perturbs cell cycle control. (medscape.com)
The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)
In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
Red blood cells contain hemoglobin, a protein that gives blood its red color and enables it to carry oxygen from the lungs and deliver it to all body tissues. (msdmanuals.com)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

Apparently these chromatophore-targeted proteins evolved convergently to plastid-targeted expression regulators and are likely involved in gene expression control in the chromatophore. (frontiersin.org)
We describe extensive use of histidine kinase-based two-component systems and tyrosine kinase signaling, the presence of bacterial and plant type photoreceptors (phytochromes, cryptochrome, and phototropin) and of plant-type pentatricopeptide repeat proteins, as well as metabolic pathways, and a cell cycle control system typically found in more complex eukaryotes. (duke.edu)
More generally, we study the reversible enzymatic coupling of proteins to other proteins within cells. (yale.edu)
The Ubl called SUMO is attached to many proteins in vivo and is crucial for cell-cycle progression. (yale.edu)
It is a contractile nanomachine which delivers toxic effector proteins directly into target cells by dynamic cycles of assembly and firing. (dundee.ac.uk)
Briefly, cells that are pre-labeled with fluorescence dye, antibodies conjugated to fluorophores, or expressing fluorescence proteins, are loaded into a stream. (bgu.ac.il)
Insertion of proteins into the bacterial outer membrane is mediated by a dedicated chaperone machinery. (unibas.ch)
It was the early 2000s, and Mukherjee was working in Dennis Shields's lab at Albert Einstein College of Medicine, studying how cells organize the internal transport of proteins and other cargo. (the-scientist.com)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

After the slide was gram stained the slide was scanned under a microscope using low power objective to locate clusters of epithelial cells. (cdc.gov)
In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)

Caption to fig.1A:) 'Temporally coordinated events of the Caulobacter cell cycle. (harvard.edu)
The dimorphic bacterium Caulobacter crescentus is a model organism for studying the bacterial cell cycle. (csun.edu)
p)ppGpp modulates cell size and the initiation of DNA replication in Caulobacter crescentus in response to a block in lipid biosynthesis. (csun.edu)
2012. The Caulobacter crescentus ctrA P1 promoter is essential for the coordination of cell cycle events that prevent the overinitiation of DNA replication. (csun.edu)
Current research examines the general principles and spatiotemporal mechanisms by which bacterial cells replicate, using Caulobacter crescentus and Escherichia coli as models. (stanford.edu)
In order to best accomplish our goals, we use the genetically tractable bacterium Caulobacter crescentus whose cell cycle can be easily synchronized to enable fine temporal resolution of each cell cycle event. (illinois.edu)
Caulobacter is famous for its dimorphic life cycle: it has two primary cell types, a motile form called the swarmer and a sessile form called the stalked cell, and it produces one of each through an obligate asymmetric cell division. (rupress.org)
In Shapiro's laboratory, Goley used Caulobacter as a model system to investigate the role of the conserved tubulin-like GTPase, FtsZ, in orchestrating bacterial division ( 3 , 4 ). (rupress.org)

Media conditions that support fast growth in bacteria also couples with shorter inter-initiation time in them, i.e. the doubling time in fast growing cells is less as compared to the slow growth. (wikipedia.org)
Bacteria dynamically regulate cell size and growth to thrive in changing environments. (nature.com)
In addition, the molecular mechanisms connecting dynamic resource allocation to division control in bacteria are not clear, nor is our understanding of how these allocation strategies are affected by the temporal pattern of environmental fluctuations. (nature.com)
As such, targeting the molecular networks involved in coordinating cell cycle events in bacteria represents an attractive target for antibiotic development. (illinois.edu)
We are interested in figuring out how bacteria integrate information from the environment into the molecular networks that drive the forward progression of the cell cycle. (illinois.edu)
Our long-term goal is to build a model that incorporates the full repertoire of factors involved in orchestrating the progression of the cell cycle in bacteria. (illinois.edu)
We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
A light-induced pH change in a cell suspension of rhodopsin-possessing bacteria was detected in the absence of exogenous retinal. (go.jp)
So-called facultative intracellular bacteria such as Listeria monocytogenes and Legionella pneumophila do not need to be inside a host cell to reproduce. (the-scientist.com)
These bacteria were often absent from gram stain smears of patients with other bacterial morphotypes. (cdc.gov)

An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)
In a study on biofilm, Stoodley (2002) showed that biofilm formation seems to be an ancient and fundamental part of the life cycles of many microorganisms and essential for survival in diverse environments. (learnlifescience.com)
Biofilm formation represents a protected mode of growth that not only allows cells to survive in hostile environments but also to colonize new niches by dispersal of microorganisms from the microbial clusters. (learnlifescience.com)
Subramani (2019) said that Quorum sensing (QS) is a process of intercellular signaling or cell-cell communication and a vital regulatory mechanism for coordinating biofilm formation including common activities and physiological processes such as symbiosis, formation of spores or fruiting bodies, antibiotics synthesis, genetic competence, apoptosis, and virulence in many bacterial species using extracellular QS signaling molecules, which is often referred to as autoinducers. (learnlifescience.com)
It is a second messenger that modulates a variety of bacterial growth phenotypes including biofilm formation. (learnlifescience.com)

Only by further elucidating the control mechanisms of bacterial cell division can we advance the development of new antimicrobial compounds. (csun.edu)

Brucella abortus and Pregnancy in Mice: Impact of Chronic Infection on Fertility and the Role of Regulatory T Cells in Tissue Colonization. (harvard.edu)
Such turnover occurs primarily through the ubiquitin-proteasome system and is central to a variety of cell regulatory mechanisms, many of medical relevance including many cancers. (yale.edu)
The ubiquitin system has only recently come under close scrutiny, and an extraordinary array of cell regulatory functions is gradually being uncovered. (yale.edu)
Bacterial cells adopt distinct post-translational regulatory strategies for deployment of the T6SS. (dundee.ac.uk)

Prokaryotic DNA Replication is the process by which a prokaryote duplicates its DNA into another copy that is passed on to daughter cells. (wikipedia.org)
All cells must finish DNA replication before they can proceed for cell division. (wikipedia.org)
Motile, piliated swarmer cells differentiate into stalked cells at the G1-S transition by shedding their polar flagellum, growing a stalk at that site, losing the polar pili, and initiating DNA replication. (harvard.edu)
CtrA is present in the shaded cells, where it represses DNA replication initiation and is cleared by proteolysis during the swarmer cell-stalked cell (G1-S) transition. (harvard.edu)
Motile swarmer cells can not undergo DNA replication until they differentiate into stationary stalked cells. (csun.edu)
Stalked cells are competent for DNA replication and cell division. (csun.edu)
This maintenance is accomplished through major cell cycle events such as chromosome replication, segregation, and proper timing of cytokinesis. (illinois.edu)
The right operon is involved in the vegetative function of the phage e.g. head synthesis, tail synthesis and DNA replication leading lytic cycle. (biologydiscussion.com)

This research aims at characterizing molecules and signals involved in controlling the undifferentiated state and self-renewal capacity of stem cells, and to develop methods to direct the differentiation of stem cells into clinically relevant cell types. (ki.se)
It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. (cancerindex.org)

We combine bacterial genetics, biochemistry, and high-resolution imaging in order to examine the progression of the cell cycle at the molecular and cellular level. (illinois.edu)

Its asymmetric cell division results in one swarmer and one stalked cell progeny. (csun.edu)

Circles and "theta" structures in the cells represent quiescent and replicating chromosomes, respectively. (harvard.edu)
X-rays or mitomycin, the prophage can be separated from bacterial chromosomes and enter the lytic cycle. (biologydiscussion.com)

Erin Goley investigates how the microbial cytoskeleton controls cell growth and division. (rupress.org)
What first drew you to study the bacterial cytoskeleton? (rupress.org)
At the time I was considering fields for postdoctoral study, in 2005, the bacterial cytoskeleton was a really new thing. (rupress.org)
I thought studying the bacterial cytoskeleton would marry my long-term interest in microbiology with the love for the cytoskeleton I acquired in graduate school, and I felt that the field was replete with fundamental mechanistic, and even phenomenological, questions. (rupress.org)

Beta globin is common to all mammalian cells and it is reasonable to expect that some human cells will be present in the sample. (cdc.gov)

In the lytic cycle, phage genes are expressed and DNA is replicated resulting in production of several phage particles. (biologydiscussion.com)
Since rhodopsin apoproteins cannot absorb light energy, rhodopsins produced by prokaryotic strains lacking genes for retinal biosynthesis are hypothesized to be non-functional in cells. (go.jp)
He also mentions that microorganisms produce a wide variety of QS signaling molecules that can be self-recognized in a concentration-dependent manner and subsequently induce or suppress the expression of QS-controlled genes. (learnlifescience.com)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)

HHVs replicate in the host cell nucleus. (medscape.com)

We found that ParA:ParB ratios fluctuate between predation stages, their balance being critical for cell cycle progression. (jic.ac.uk)
Ultimately, IFNGR1 mediated signaling regulates several biological processes including innate and acquired immune response, apoptosis and cell cycle progression. (rndsystems.com)
Our work is exposing the complex and multidirectional communicative systems involved in coordinating the progression of the cell cycle. (illinois.edu)
Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. (molcells.org)

Studying bacterial interactions with actin is still throwing up new mysteries. (the-scientist.com)

Thus, the mechanism generating metabolic connectivity of the chromatophore fundamentally differs from the one for mitochondria and plastids, but likely rather resembles the poorly understood mechanism in various bacterial endosymbionts in plants and insects. (frontiersin.org)
CONCLUSIONS- This new finding demonstrates that changes in gut microbiota controls metabolic endotoxemia, inflammation, and associated disorders by a mechanism that could increase intestinal permeability. (diabetesjournals.org)

In other words, it is possible that in fast growth conditions the grandmother cells starts replicating its DNA for grand daughter cell. (wikipedia.org)
While previous studies have characterized bacterial growth physiology at steady-state, a quantitative understanding of bacterial physiology in time-varying environments is lacking. (nature.com)
Here we develop a quantitative theory connecting bacterial growth and division rates to proteome allocation in time-varying nutrient environments. (nature.com)
In such environments, cell size and growth are regulated by trade-offs between prioritization of biomass accumulation or division, resulting in decoupling of single-cell growth rate from population growth rate. (nature.com)
As a result, it remains unclear how cells sense changes in the environment and dynamically regulate division and growth in response. (nature.com)
To understand the dynamics of bacterial growth physiology and size control in dynamic nutrient environments, we have developed a coarse-grained proteome sector model which connects gene expression to growth rate and division control, and accurately predicts the cell-level E. coli response to nutrient perturbations in both exponential and stationary phase seen in experimental data 5 , 25 . (nature.com)
The development of such a model has the potential to be transformative in our ability to control bacterial growth. (illinois.edu)
Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
and Goley took her new favorite bug to Johns Hopkins University to establish her own research program tackling the question of how bacterial cell growth and division are controlled by FtsZ. (rupress.org)
Cancer cells obtain a growth advantage through uncontrolled cell proliferation ( Hanahan and Weinberg, 2011 ), which may be caused by mutations that help them adapt to the microenvironment through selective pressure. (molcells.org)

Laub MT, McAdams HH, Feldblyum T, Fraser CM, Shapiro L. Global analysis of the genetic network controlling a bacterial cell cycle. (harvard.edu)
Caulobacter's obligate cell cycle is controlled by oscillating master regulators that control different genetic modules in space and time. (csun.edu)
Much of our work is conducted in baker's yeast, a model organism ideal for genetic and biochemical analysis, but we also use human tissue culture cells and Drosophila in certain studies. (yale.edu)

At least five five randomized, placebo-controlled studies have shown that prolonged courses of IV antibiotics do not improve long-term outcome for patients with a diagnosis of chronic Lyme disease (e.g., this one ). (scienceblogs.com)

In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. (molcells.org)
Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. (molcells.org)
In this study, we investigated how TSPAN12 regulates OC cell proliferation both in vitro and in vivo and found that it contributed to tumor proliferation and poor prognosis in this disease through cyclin and cyclin-dependent kinase (CDK) pathways. (molcells.org)

Cell populations can also be sorted and collected into separate tubes. (bgu.ac.il)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

The oil immersion lens (x1000) was switched and between 10 and 20 representative fields was examined to observe cell morphology and gram reaction. (cdc.gov)

2017). Endoplasmic reticulum chaperone Gp96 controls actomyosin dynamics and protects against pore-forming toxins . (up.pt)

MATERIALS AND METHODS: In this study, the chemical constituents in SJZT were analyzed by UPLC-Q-Exactive-MS/MS. MTT and cell scratch test were used to determine the cell viability and inhibition of migration in vitro. (bvsalud.org)

These findings imply that similar to the situation in mitochondria and plastids, also in P. chromatophora nuclear factors evolved that control metabolite exchange and gene expression in the chromatophore. (frontiersin.org)

2011). The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle . (up.pt)

Lipid biosynthesis is essential for cell viability and bacterial fatty acid synthetic enzymes have been suggested as antibiotic targets. (csun.edu)

Life Cycles of Phage Lambda 4. (biologydiscussion.com)
The phage λ leads two life cycles, the lytic cycle and the lysogenic cycle after injecting its DNA into E.coli cell. (biologydiscussion.com)

We want to derive quantitative predictors for chaperone function, unravel atomic-level details of how clients are recognized, understand how the interplay works between chaperone and client dynamics and study how basic chaperone function is embedded into complex functional cycles. (unibas.ch)
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)

Through detailed characterization of fundamental molecular mechanisms of normal and perturbed cells, the theme strives to unravel information that increases the understanding of various diseases such as cancer and neurodegenerative, cardiovascular and inflammatory disorders. (ki.se)
She is interested in understanding the fundamental mechanisms and principles by which cells, and, in particular, bacterial cells, are able to multiple. (stanford.edu)

Most previous studies on bacterial lipid metabolism have focused on E. coli , a gamma-proteobacteria. (csun.edu)
Phage lambda is a virus of E. coli K12 which after entering inside host cell normally does not kill it in-spite of being capable of destroying the host. (biologydiscussion.com)
The 12 nucleotides of cohesive ends and process of circularization are shown in Fig. 18.11B-C. The events of circularization occurs after injection of phage DNA into E.coli cell where the bacterial enzyme, i.e. (biologydiscussion.com)
The lytic cycle ends with lysis of E.coli cells and liberation of phage particles. (biologydiscussion.com)

RESEARCH DESIGN AND METHODS- We changed gut microbiota by means of antibiotic treatment to demonstrate, first, that changes in gut microbiota could be responsible for the control of metabolic endotoxemia, the low-grade inflammation, obesity, and type 2 diabetes and, second, to provide some mechanisms responsible for such effect. (diabetesjournals.org)
The aim of this study was to investigate the inhibitory effects of Cordyceps militaris solid medium extract (CME) and cordycepin (COR) on LTA-induced inflammation in MH-S cells and their mechanisms of action. (bvsalud.org)
a bacterial induced inflammation of the lining of the heart and its valves. (cdc.gov)

Cell lysis as a feature of biofilm development. (southampton.ac.uk)

Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

We previously determined, first, that metabolic endotoxemia controls the inflammatory tone, body weight gain, and diabetes, and second, that high-fat feeding modulates gut microbiota and the plasma concentration of lipopolysaccharide (LPS), i.e., metabolic endotoxemia. (diabetesjournals.org)

Key areas of investigation are the identification and functional characterization of stem cell niches in developing tissues and adult organs. (ki.se)

Swarmer cells from wild-type C. crescentus were isolated and allowed to proceed synchronously through their 150-min cell cycle (Fig. 1A). (harvard.edu)
Cell division yields distinct progeny, a swarmer cell and a stalked cell. (harvard.edu)

Bacterial urease converts urea to ammonium and bicarbonate, neutralizing gastric acid and providing protection in the hostile, highly acidic gastric environment. (medscape.com)

Important adaptive features that enhance survival of the organism in an acidic environment include its shape and motility, its reduced oxygen requirement, its adhesion molecules that are trophic to certain gastric cells, and its urease production. (medscape.com)

8. Melchers, F.: Biosynthesis, transport and secretion of immunoglobulin in plasma cells. (uni-mainz.de)

Screening of yeast or bacterial display systems. (bgu.ac.il)

It has a sophisticated life cycle with several distinct stages including amoebal, flagellated, and plasmodial cells. (duke.edu)

Centers for Disease Control and the United States ( 8 ). (cdc.gov)
Courtesy of Centers for Disease Control and Prevention (CDC). (medscape.com)

A new factor controlling cell envelope integrity in Alphaproteobacteria in the context of cell cycle, stress response and infection. (harvard.edu)
[ 5 , 6 ] In a localized primary infection, the virus penetrates the mucosal epithelium and invades the cells of the basal layer, where the viral DNA inserts into the host DNA. (medscape.com)

During cell division, a flagellum is placed at the pole opposite that of the stalk. (csun.edu)
As a result of this carefully orchestrated process, a flagellum is synthesized only when needed (just prior to cell division) and is placed at the pole opposite that of the stalk. (csun.edu)
This allows for faster adaptation to previously seen environments and results in division control which is dependent on the time-profile of fluctuations. (nature.com)

Cell-matrix adhesion regulates membrane trafficking controlling anchorage-dependent signaling. (molvisindex.org)

2015. Versatility of global transcriptional regulators in alpha-Proteobacteria: from essential cell cycle control to ancillary functions. (csun.edu)
Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. (learnlifescience.com)

As a trade-off, they have had to come up with ways to bypass a cell's internal immune system, navigate the complicated, busy environment of the cell cytoplasm, and ultimately escape that environment to infect other cells-becoming tiny maestros of manipulation in the process. (the-scientist.com)

Allosteric control of a bacterial stress response system by an anti-s factor. (harvard.edu)
In developing this coculture system, we needed to add PFC neurons in sufficient number to restore glutamate input to 1-Methylinosine NAc neurons while at the same time maintaining a cell density sufficiently low to allow image analysis of single neurons. (bakingandbakingscience.com)
The fluidics system in the BD FACSAria™ III cell sorter is pressure driven. (bgu.ac.il)

In a biofilm, microbial cells stick to each other and often also to a surface. (learnlifescience.com)

In addition to other benefits, the next generation flow cell in the BD FACSAria III is designed to improve resolution for side population applications and DNA cell cycle analyses. (bgu.ac.il)

Anatomy26

Organisms13

Diseases4

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes84

Disciplines and Occupations1

Information Science4

Health Care2

Eukaryotic12

Pathogens7

Biology14

Regulation11

Protein20

Proteins9

Epithelial2

Caulobacter8

Bacteria10

Biofilm formation5

Mechanisms of bacterial1

Regulatory4

Replication8

Differentiation2

Genetics1

Asymmetric cell div1

Chromosomes2

Cytoskeleton4

Mammalian cells1

Genes4

Replicate1

Progression4

Actin1

Mechanism2

Growth10

Genetic3

Antibiotics1

Proliferation4

Populations2

Morphology1

Toxins1

Inhibition1

Gene1

Virulence1

Viability1

Life-cycles2

Quantitative2

Fundamental2

Coli4

Inflammation3

Lysis1

Organisms1

Modulates1

Characterization1

Swarmer2

Converts1

Molecules1

Biosynthesis1

Yeast1

Distinct1

Centers2

Infection2

Division3

Regulates1

Regulators2

Cytoplasm1

System3

Microbial1

Analyses1