• In obesity, adipose tissue is also implicated in the chronic release of pro-inflammatory markers known as adipokines, which are responsible for the development of metabolic syndrome, a constellation of diseases, including type 2 diabetes, cardiovascular disease and atherosclerosis. (wikipedia.org)
  • In obesity the inherent capacity of adipose tissue to store and sense nutrients is compromised, causing spillover of the intermediate lipid metabolites into circulation and resulting in their ectopic deposition in tissues not suitable for lipid storage, a phenomenon known as lipotoxicity. (frontiersin.org)
  • The aim of this study was to determine whether the mean size of fat cells in either visceral or subcutaneous adipose tissue has an impact on the metabolic and inflammatory profiles in morbid obesity. (nih.gov)
  • Hyperplasia (many small adipocytes) in both adipose regions may be protective against lipid as well as glucose/insulin abnormalities in obesity. (nih.gov)
  • Adipose tissue in obesity becomes refractory to suppression of fat mobilization by insulin, and also to the normal acute stimulatory effect of insulin on activation of lipoprotein lipase (involved in fat storage). (cambridge.org)
  • Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
  • The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
  • Remarkably, dietary selenium supplementation elevated facultative incorporation into UCP1, elevated energy expenditure through thermogenic adipose tissue, and protected against obesity. (nih.gov)
  • Excess adipose tissue, resulting in obesity, is currently one of the most serious threats to human health on a global level. (europa.eu)
  • Exceeding the capacity to store TG in adipocytes occurs in obesity and is often accompanied by deposition of TG in other tissues and metabolic diseases, such as diabetes mellitus or non-alcoholic fatty liver disease. (elifesciences.org)
  • Extracellular matrix (ECM) remodelling from the adipose tissue plays a pivotal role in the pathophysiology of obesity. (exposed-skin-care.net)
  • Inhibition of COX activity attenuated diet-induced UCP1 expression and increased energy efficiency and adipose tissue mass in obesity-resistant mice kept at thermoneutrality. (ku.dk)
  • Our results indicate that cyclooxygenase-dependent induction of UCP1 expression in white adipose tissues is important for diet-induced thermogenesis providing support for a surprising role of COX activity in the control of energy balance and obesity development. (ku.dk)
  • Objective: β-adrenoceptor mediated activation of brown adipose tissue (BAT) has been associated with improvements in metabolic health in models of type 2 diabetes and obesity due to its unique ability to increase whole body energy expenditure, and rate of glucose and free fatty acid disposal. (monash.edu)
  • White adipose tissue (WAT) is the principal organ in obesity. (nature.com)
  • Vitamin D accumulates in adipose tissue (AT), and vitamin D deficiency is frequent in obesity. (medscape.com)
  • The high prevalence of vitamin D deficiency in obese subjects [ 3 ] suggests that adipose tissue (AT) has a major role in the body distribution of vitamin D, but the causal relationship between obesity and low levels of circulating 25(OH)D has not been completely elucidated yet. (medscape.com)
  • Role of chronic inflammation in adipose tissue in the pathophysiology of obesity]. (elsevierpure.com)
  • Rare adipose disorders (RADs) masquerading as obesity. (medlineplus.gov)
  • Adipose tissue cellularity in human obesity. (bvsalud.org)
  • In humans, adipose tissue is located: beneath the skin (subcutaneous fat), around internal organs (visceral fat), in bone marrow (yellow bone marrow), intermuscular (Muscular system) and in the breast (breast tissue). (wikipedia.org)
  • Adipose cell size (lipid per cell) was determined in three different subcutaneous and deep fat depots in each patient and the total number of adipose cells in the body estimated by division of total body fat by various combinations of the adipose cell sizes at six different sites. (jci.org)
  • A transcriptomic research revealed a rise in LOX manifestation in subcutaneous white adipose cells from obese topics, but its pathophysiological relevance continues to be unclear (Henegar et al. (exposed-skin-care.net)
  • We aimed to determine whether DNA methylation and mRNA levels of the ABCA1 and ABCG1 genes in EAT and subcutaneous adipose tissue (SAT) were associated with CAD. (biomedcentral.com)
  • iXCells Biotechnologies provides SVF from human subcutaneous white fat tissue by enzymatic isolation and cryopreserved at P0, with ≥ 1.0 million cells/vial (Normal) or 0.5 million cells/vial (Diseased). (ixcellsbiotech.com)
  • Short- and long-term glucocorticoid treatment enhances insulin signalling in human subcutaneous adipose tissue. (ox.ac.uk)
  • Visceral Adipose Tissue (VAT) and subcutaneous Adipose Tissue (SAT) were defined by the Hologic APEX software used in the scan analysis. (cdc.gov)
  • Adipose tissue (also known as body fat, or simply fat) is a loose connective tissue composed mostly of adipocytes. (wikipedia.org)
  • In addition to adipocytes, adipose tissue contains the stromal vascular fraction (SVF) of cells including preadipocytes, fibroblasts, vascular endothelial cells and a variety of immune cells such as adipose tissue macrophages. (wikipedia.org)
  • Apart from adipocytes, which comprise the highest percentage of cells within adipose tissue, other cell types are present, collectively termed stromal vascular fraction (SVF) of cells. (wikipedia.org)
  • Adipocytes, the key components of the adipose tissue, have unique ability to store excess energy in the form of triglycerides, sense systemic energy demands, and secrete factors (lipids, peptides, cytokines, and adipokines) to regulate other metabolic tissues. (frontiersin.org)
  • The presence of various types of adipocytes (white, brown, and beige) characterized by the number/size of lipid droplets, mitochondrial density, and thermogenic capacity, further highlights how intricate is the communication of these cell-types with other metabolic tissues to sense nutrients. (frontiersin.org)
  • The present article discusses how accumulation of triacylglycerol in adipocytes can lead to deterioration of the responsiveness of glucose metabolism in other tissues. (cambridge.org)
  • In humans the generation of fat cells (adipocytes) is a major factor behind the growth of adipose tissue during childhood. (europa.eu)
  • 5 , - , 9 This loose connective tissue is composed of extracellular matrix and various types of cells: fat cells-named adipocytes, and the non-fat cells of the stromal vascular fraction that contains preadipocytes, capillary endothelial cells, infiltrating leucocytes and multipotent stem cells. (bmj.com)
  • The WAT-on-a-chip is a multilayer device that features tissue chambers tailored specifically for the maintenance of 3D tissues based on human primary adipocytes, with supporting nourishment provided through perfused media channels. (nature.com)
  • Endocrine functions of white adipocytes, and other WAT-resident cells in the stromal vascular fraction, are performed by the release of a variety of adipokines (adipose-associated cytokines) which affect the functioning of the brain, liver, pancreas and immune system 9 . (nature.com)
  • Most adipocytes exist in discrete depots throughout the body, notably in well-defined white and brown adipose tissues. (wustl.edu)
  • Since skin-embedded adipocytes may provide natural insulation, they provide an important counterpoint to the activation of thermogenic brown and beige adipose tissues, whereby these distinct depots are functionally interrelated and require simultaneous assay. (jci.org)
  • This study will be of high interest to those in the adipose tissue biology and metabolism fields. (elifesciences.org)
  • Adipose tissue plays an important role in metabolism through its storage and release of triglycerides, peptide hormones (adipokines) and other proteins, and in the case of brown fat, for its role in thermogenesis ( 1 ). (diabetesjournals.org)
  • Cyclooxygenases are known as important regulators of metabolism and immune processes via conversion of C20 fatty acids into various regulatory lipid mediators, and cyclooxygenase activity has been implicated in browning of white adipose tissues. (dtu.dk)
  • It can also help increase the production and metabolism of brown adipose tissues, which is crucial for burning calories. (reducedfatgirl.com)
  • Dermal white adipose tissue (dWAT) was recently recognized for its potential to modify whole body metabolism. (jci.org)
  • In addition, they have revealed the drop of a specific enzyme involved in cellular metabolism in the adipose tissues and blood, as well as in the nucleus accumbens, a brain region regulating motivated behaviors. (stressnetwork.ch)
  • which produces hormones), or changes in the deposition and breakdown of fat (adipose tissue metabolism). (medlineplus.gov)
  • Besides, a role could be ascribed to molecules secreted by the visceral adipose tissue that can impact on barrier functions. (hindawi.com)
  • Total body adiposity correlates with the amount of epicardial adipose tissue (EAT), a specific visceral fat accumulation around the myocardium in the proximity to the coronary arteries [ 2 ]. (biomedcentral.com)
  • Visceral adipose tissue area, mass and volume of fat inside abdominal cavity were measured at the approximate interspace location of L4 and L5 vertebra. (cdc.gov)
  • abstract = "Tissue-engineered constructs need to become quickly vascularized in order to ensure graft take. (vu.nl)
  • The use of PGE(2) receptor antagonists implicated EP(4) as a main PGE(2) receptor, and injection of the stable PGE(2) analog (EP(3/4) agonist) 16,16 dm PGE(2) induced UCP1 expression in inguinal white adipose tissue. (ku.dk)
  • Compared to littermate wildtype mice, TG mice selectively reduced inguinal white adipose tissue (iWAT) mass and fat cell size, whereas the epididymal (eWAT) fat depot remained unchanged. (dtu.dk)
  • Notably, recent studies suggest that the accumulation of sphingolipids, namely ceramides and it's metabolites, play essential roles in the development of insulin resistance in tissues such as skeletal muscle, liver and, adipose tissue in obese rodents, and humans ( 6 - 20 ). (frontiersin.org)
  • Moreover, we hypothesized that these mice would have accumulation of TGs in other tissues, such as the liver or skeletal muscle, resulting in lipotoxicity and metabolic derangements, such as insulin resistance or fatty liver disease. (elifesciences.org)
  • The Adipoq-Cre exhibited no observable recombination in any other tissues examined, whereas both aP2-Cre lines resulted in recombination in endothelial cells of the heart and nonendothelial, nonmyocyte cells in the skeletal muscle. (diabetesjournals.org)
  • Although GCs cause global insulin resistance in vivo, we have previously shown that GCs are able to augment insulin action in human adipose tissue, contrasting with their action in skeletal muscle. (ox.ac.uk)
  • Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the development of coronary artery disease (CAD). (biomedcentral.com)
  • However, its main function is to be a reserve of lipids, which can be oxidised to meet the energy needs of the body and to protect it from excess glucose by storing triglycerides produced by the liver from sugars, although some evidence suggests that most lipid synthesis from carbohydrates occurs in the adipose tissue itself. (wikipedia.org)
  • Thus, in the postprandial period especially, there is an excess flux of circulating lipid metabolites that would normally have been 'absorbed' by adipose tissue. (cambridge.org)
  • Conversely, brown adipose tissue (BAT) and browning of WAT represent potential therapeutic approaches, since dysfunctional white adipocyte-induced lipid overspill can be halted by BAT/browning-mediated oxidative anti-lipotoxic effects. (springer.com)
  • There is also evidence that the deleterious effects mediated by dysfunctional white adipocyte-induced lipid overspill can be halted by the pro-oxidative anti-lipotoxic effects mediated by brown adipose tissue (BAT) activation. (springer.com)
  • Whereas bone marrow adipose tissue (BMAT) shares many properties in common with white adipose tissue, the distinct functions of BMAT are reflected by its development, regulation, protein secretion, and lipid composition. (wustl.edu)
  • However, isolation of WAT and brown adipose tissue (BAT) is technically challenging due to their high lipid and low protein contents. (inventbiotech.com)
  • Although this lipid layer has been frequently grouped together with s.c. white adipose tissue (sWAT), it is now recognized as a distinct depot that responds to different stimuli. (jci.org)
  • Accumulation of neutral lipids, such as triglycerides, in key insulin target tissues, has been postulated to inhibit metabolic functions, however, they are less likely to be deleterious. (frontiersin.org)
  • The researchers found that 50 of the subjects had an inflamed adipose phenotype characterized by tissue macrophage accumulation in crown-like structures. (massagemag.com)
  • Bioaccumulation - accumulation in adipose tissue of aquatic organisms. (janusinfo.se)
  • Because energy sources are not always available from the environment, many metazoan organisms have evolved the ability to store large amounts of metabolic energy as triglycerides (TG) in adipose tissue. (elifesciences.org)
  • Adipose tissue is found in specific locations, which are referred to as adipose depots. (wikipedia.org)
  • Adipose depots in different parts of the body have different biochemical profiles. (wikipedia.org)
  • Mice have eight major adipose depots, four of which are within the abdominal cavity. (wikipedia.org)
  • Both the mesenteric and omental depots incorporate much lymphoid tissue as lymph nodes and milky spots, respectively. (wikipedia.org)
  • The two superficial depots are the paired inguinal depots, which are found anterior to the upper segment of the hind limbs (underneath the skin) and the subscapular depots, paired medial mixtures of brown adipose tissue adjacent to regions of white adipose tissue, which are found under the skin between the dorsal crests of the scapulae. (wikipedia.org)
  • The postdoctoral project will investigate the factors driving senescence in human adipose tissue and why specific adipose depots are protected from senescence. (varbi.com)
  • In morbidly obese women region-specific variations in mean adipocyte size are associated with metabolic complications but not systemic or adipose inflammation. (nih.gov)
  • As senior investigator Dr. Noyan Gokce told Reuters Health, "Our data demonstrate that obese subjects exhibit varying degrees of inflammation in their fat tissue, and increased inflammatory activity in fat was associated with systemic metabolic abnormalities including insulin resistance. (massagemag.com)
  • Because adipose inflammation was linked to impaired arterial endothelial function, Dr. Gokce pointed out, "that may identify obese individuals at increased cardiovascular risk. (massagemag.com)
  • More recent studies suggest that inflammation in adipose tissue could be a key factor leading to these illnesses. (bu.edu)
  • 1 In RA, numerous cytokines play a fundamental role in inflammation and joint destruction and are thought to originate primarily from synoviocytes and leucocytes that massively infiltrate synovial tissue. (bmj.com)
  • analyses, as well as others (8-11), support the hypothesis that adipose tissue-secreted factors may indeed be potent modulators of inflammation-related disorders such as obe- sity, type 2 diabetes, metabolic syndrome, atherosclerosis, inflammatory bowel disease, thyroid-associated (Graves') ophthalmopathy, breast cancer, and nonalcoholic fatty liver disease. (123dok.org)
  • Adipose tissue, especially white adipose tissue (WAT), has been recognized as an essential endocrine and inflammation organ in addition to its energy storage function. (inventbiotech.com)
  • Oxidation of cysteine thiols by physiological reactive oxygen species (ROS) initiates thermogenesis in brown and beige adipose tissues. (nih.gov)
  • Researchers have found that a traditional West African herbal remedy called Grains of Paradise increases thermogenesis and stimulates the loss of brown adipose fat - the inflammatory type of fat. (plantmedicines.org)
  • This situation leads to fat deposition in other tissues. (cambridge.org)
  • Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity. (inventbiotech.com)
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
  • In this study, the authors examined combinations of growth factors that induce effective chondrogenesis from adipose tissue-derived mesenchymal stem cells (MSCs). (cellsurgicalnetwork.com)
  • The cellular character of the adipose tissue of 21 nonobese and 78 obese patients has been examined. (jci.org)
  • The total proteins isolated are the unbiased representation of cellular proteins in the tissue. (inventbiotech.com)
  • Adipose tissue exhibits remarkable plasticity with capacity to change in size and cellular composition under physiological and pathophysiological conditions. (bvsalud.org)
  • Here, we present a comprehensive overview of the cellular atlas of adipose tissues focusing on the biological insight gained from single- cell and single-nuclei transcriptomics of murine and human adipose tissues . (bvsalud.org)
  • Far from being hormonally inert, adipose tissue has, in recent years, been recognized as a major endocrine organ, as it produces hormones such as leptin, estrogen, resistin, and cytokines (especially TNFα). (wikipedia.org)
  • Tissue explants were treated with proinflammatory cytokines relevant to RA pathogenesis (interleukin 1β (IL-1β), tumour necrosis factor (TNF), interferon γ, IL-15, IL-17, IL-23). (bmj.com)
  • Conclusion Rheumatoid AAT is highly reactive tissue which upon stimulation secretes considerable amounts of proinflammatory (IL-6, IL-8, TNF) and anti-inflammatory (IL-1Ra) cytokines and classical adipokines. (bmj.com)
  • Recently, adipose tissue has been shown to synthesise and release highly bioactive substances: classical adipokines (eg, leptin, adiponectin) and various proinflammatory factors such as cytokines, chemokines, growth factors and complement components. (bmj.com)
  • In effect, a wide range of exported secretory proteins, dubbed adipokines, have been identified as constituents of the adipose proteome (adipokinome). (123dok.org)
  • White adipose tissue (WAT) has key metabolic and endocrine functions and plays a role in regulating energy homeostasis and insulin sensitivity. (springer.com)
  • White adipose tissue (WAT) plays a key homeostatic role, not only by ensuring efficient energy storage but also by its quick mobilisation (lipids) to ensure peripheral demands. (springer.com)
  • The favorable metabolic phenotype resulted from activation of energy expenditure, in part via BAT activation and beiging of white adipose tissue. (elifesciences.org)
  • Furthermore, in white adipose cells from obese pets, BAPN avoided the downregulation of adiponectin and blood sugar transporter 4 (GLUT4), aswell as the upsurge in suppressor of cytokine signaling 3 (SOCS3) and dipeptidyl peptidase 4 (DPP4) amounts, triggered from the HFD. (exposed-skin-care.net)
  • CONCLUSIONS/SIGNIFICANCE: Our findings provide evidence that induction of UCP1 expression in white adipose tissue, but not in classic interscapular brown adipose tissue is dependent on cyclooxygenase activity. (ku.dk)
  • Particularly well developed in humans is white adipose tissue (WAT), a major meta- bolic and secretory organ. (123dok.org)
  • White adipose tissue (WAT) - a key contributor in many metabolic diseases - contributes about one fourth of a healthy human's body mass. (nature.com)
  • and plasma membrane properties and receptors in white adipose tissue. (elsevier.com)
  • In lipodystrophy there is insufficient adipose tissue to absorb the postprandial influx of fatty acids, so these fatty acids will again be directed to other tissues. (cambridge.org)
  • They are characterized by their bioaccumulation properties in the fatty parts of biological beings, such as blood, breast milk and fatty tissues via the food chain ( Smith and Gangolli, 2002 ). (scialert.net)
  • The supplemented fatty acids were increased in different muscle and adipose tissues of the five-day-old calves as a result of maternal transfer. (uni-rostock.de)
  • Overall, no major effects of the altered fatty acid supply on muscle and adipose tissue development were observed. (uni-rostock.de)
  • Adiposis dolorosa is a condition characterized by painful folds of fatty (adipose) tissue or the growth of multiple noncancerous (benign) fatty tumors called lipomas. (medlineplus.gov)
  • In people with adiposis dolorosa, abnormal fatty tissue or lipomas can occur anywhere on the body but are most often found on the torso, buttocks, and upper parts of the arms and legs. (medlineplus.gov)
  • Objectives (1) To compare spontaneous and stimuli-induced adipocytokine secretion by articular adipose tissue (AAT) and synovial membrane (SM) explants obtained from patients with rheumatoid arthritis (RA). (bmj.com)
  • After stimulation of adipose-EC and dermal-EC with TNF-α an increased secretion was seen for PDGF-BB, but not uPA, PAI-1 or Angiopoietin-2. (vu.nl)
  • Relative abundance of organochlorine pesticides and polychlorinated biphenyls in adipose tissue and serum of women in Long Island, New York. (aacrjournals.org)
  • It is important to know whether adipose tissue used by some investigators and serum assays used by others can provide comparable information on body burden. (aacrjournals.org)
  • Concentrations of seven OCPs or their breakdown products as well as 14 PCB congeners were measured in the adipose tissue and serum of 293 women enrolled as controls in a case-control study of environmental factors for breast cancer in Long Island, New York, a high-risk region. (aacrjournals.org)
  • 1,1-Dichloro-2,2-di(4-chlorophenyl)ethylene comprised 77% of total pesticide residues in adipose and 71% in serum. (aacrjournals.org)
  • All adipose-serum correlations for pesticides and most PCBs were statistically significant. (aacrjournals.org)
  • Either serum or adipose OCP/PCB levels of a variety of environmental organochlorine compounds may serve as useful biomarkers of body burden. (aacrjournals.org)
  • [ 5 , 6 ] An alternative hypothesis considers low serum 25(OH)D in obese as a result of volumetric dilution of vitamin D in the large adipose stores. (medscape.com)
  • In addition, macrophage retention in fat was associated with upregulated tissue CD68 and tumor necrosis factor mRNA expression and with increased plasma high sensitivity C-reactive protein. (massagemag.com)
  • HSL from chicken adipose tissue exhibits remarkable activation upon phosphorylation with cAMP-dependent protein kinase (cAMP-PK) compared to HSL from rat and human adipose tissue. (lu.se)
  • Also, in these pets, BAPN ameliorates the disruptions in the adipose cells manifestation of adiponectin, blood sugar transporter 4 (GLUT4), buy 76584-70-8 suppressor of cytokine signaling 3 (SOCS3) and dipeptidyl peptidase 4, all protein mixed up in control of insulin level of sensitivity. (exposed-skin-care.net)
  • Expertise in the fields of cell signalling, including protein-protein interations and adipose tissue biology is required. (varbi.com)
  • A. SDS-PAGE (10%) profiles of total protein extracted from different adipose tissues. (inventbiotech.com)
  • High Efficiency Protein Extraction from Adipose Tissues. (inventbiotech.com)
  • To evaluate the immunoexpression of BMP-2 protein on the bone repairing of critical size defects (CSD) surgically created in rat calvaria and treated by autogenous macerated adipose tissue. (bvsalud.org)
  • Adipose tissue - more specifically brown adipose tissue - was first identified by the Swiss naturalist Conrad Gessner in 1551. (wikipedia.org)
  • Its formula includes ingredients that help raise the levels of brown adipose tissue (BAT). (reducedfatgirl.com)
  • In adult humans, brown adipose tissue is very scarce and probably not functional. (123dok.org)
  • the MRI technique we describe uses an advanced fat-specific method to measure the thickness of dWAT, together with the total volume of WAT and the relative activation/fat depletion of brown adipose tissues (BAT). (jci.org)
  • The adipose tissue stromal vascular fraction (adipose-SVF) might provide an alternative source for endothelial cells as adipose tissue can easily be obtained by liposuction. (vu.nl)
  • Since adipose-EC are now gaining more interest in tissue engineering, we aimed to extensively characterize endothelial cells from adipose tissue (adipose-EC) and compare them with endothelial cells from dermis (dermal-EC). (vu.nl)
  • Adipose- and dermal-EC expressed the typical endothelial markers PECAM-1, ICAM-1, Endoglin, VE-cadherin and VEGFR2 to a similar extent, with 80-99% of the cell population staining positive. (vu.nl)
  • In conclusion, we show here that adipose tissue provides for an excellent source of endothelial cells for tissue engineering purposes, since they are readily available, and easily isolated and amplified. (vu.nl)
  • NEW YORK (Reuters Health) - Certain obese people demonstrate potentially harmful proinflammatory changes in their adipose tissue, researchers report in the September issue of Arteriosclerosis, Thrombosis, and Vascular Biology. (massagemag.com)
  • It is our goal to establish an ARC focused on understanding the relationship between metabolic disorders and Adipose Tissue Biology by studying adipose tissues from insulin sensitive and insulin resistant patients obtained during and after Bariatric Surgery. (bu.edu)
  • With collective efforts of faculty representing different expertise and disciplines, we are posed to begin the development at BUMC of a unique center of knowledge in the field of adipose tissue biology pre- and -post bariatric surgery. (bu.edu)
  • Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. (diabetesjournals.org)
  • Thus, different "adipocyte-specific" Cre lines display different degrees of efficiency and specificity, illustrating important differences that must be taken into account in their use for studying adipose biology. (diabetesjournals.org)
  • The Department of Cell and Molecular Biology (CMB, https://ki.se/en/cmb/department-of-cell-and-molecular-biology) is one of five departments conducting advanced experimental research under one roof in the new building of Biomedicum, Karolinska Institutet (https://ki.se), one of Europe's largest laboratories with ultramodern facilities. (varbi.com)
  • The Spalding lab uses pioneering approaches such as single cell sequencing, radiocarbon dating, confocal microscopy and flow cytometry to investigate fundamental questions in human adipose tissue biology. (varbi.com)
  • The Spalding research group has developed a strong competency of working with human adipose tissue, developing novel technologies and methodologies such as single cell sequencing, flow cytometry, confocal microscopy and cell culture, to address pressing questions in human adipose tissue biology. (varbi.com)
  • Knowledge of immunocytochemistry, confocal microscopy, primary tissue culture, flow cytometry, molecular biology (including gene editing) or next generation sequencing (such as RNA-seq) is an advantage. (varbi.com)
  • Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation. (ixcellsbiotech.com)
  • Center for Functional Genomics and Tissue Plasticity, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark. (bvsalud.org)
  • In obese rats, the inhibition of LOX activity through -aminopropionitrile (BAPN, a particular inhibitor of LOX activity) decreases adipose cells fibrosis, partly corrects the adipocyte-size distribution design (moving it toward smaller sized sizes) and attenuates the upsurge in bodyweight and extra fat mass. (exposed-skin-care.net)
  • Initial studies of bariatric surgery patients by the Ruderman laboratory established (1) that they could be subdivided into insulin resistant (75%) and insulin sensitive (25%) subgroups and (2) that these subgroups were distinguished by differences in AMPK activity, oxidative stress and the expression of inflammatory, mitochondrial and other genes in their adipose tissue. (bu.edu)
  • Viral DNA was isolated from brain, lung, liver, muscle, and adipose tissue of monkeys 7 months after initial infection, long after the active virus has disappeared from blood and feces ( 3 ). (cdc.gov)
  • The current proposal aims to determine the dynamics of human adipose tissue maintenance and investigate any differences in regulation of the fat mass in lean and obese individuals. (europa.eu)
  • Biopsy of adipose tissue identified human adenovirus 36 (Adv 36) DNA. (cdc.gov)
  • BioChain's PCR Ready First Strand cDNAs are available from different human adult normal tissues. (biochain.com)
  • First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. (biochain.com)
  • The writers demonstrate that LOX may be the primary lysyl oxidase isoenzyme indicated in Rabbit polyclonal to ZFP161 human being adipose cells and that it's upregulated in examples from both obese people and rats given a high-fat diet plan. (exposed-skin-care.net)
  • The levels of organochlorine residues in 40 samples of milk and 20 human adipose tissues from two locations in the northern of Côte d Ivoire were determined. (scialert.net)
  • Human breast milk samples (40 samples) and adipose tissues (20 samples) were collected from mothers (17 to 56 years old, average age 30) living in two different locations in the north of Côte d Ivoire (Korhogo and Sinématiali) from 2004 to 2007. (scialert.net)
  • Preliminary studies identify insulin as a key driver of senescence and this project aims to elucidate the insulin signalling pathways involved in this phenomenon (by investigating the molecular pathway/s promoting insulin-stimulated senescence in human adipose tissue). (varbi.com)
  • these systems integrate engineered human tissues into physiological microenvironment supplied by a vasculature-like microfluidic perfusion. (nature.com)
  • Human MSCs were isolated from bone marrow (BMMSCs) and adipose tissue (ATMSCs) and characterized according to flow cytometry for CD34, CD45, CD73, and CD166. (cellsurgicalnetwork.com)
  • Stromal vascular progenitors in adult human adipose tissue. (ixcellsbiotech.com)
  • Although we have added all of the meanings of Adipose tissue with utmost care but there could be human errors in the translation. (meaningin.com)
  • To generate mice lacking TGs in adipose tissue (ADGAT DKO), we crossed adipose tissue-specific Dgat1 knockout mice (Cre-transgene expressed under control of the mouse adiponectin promoter 3 ) with Dgat2 flox mice 4 . (elifesciences.org)
  • Decellularized adipose tissue scaffolds for soft tissue regeneration a" by Pascal Morissette Martin, Arthi Shridhar et al. (uwo.ca)
  • The adipose tissue is an important reservoir of adult stem cells which have capacity of differentiating in osteoblasts with potential implication in reaching bone regeneration. (bvsalud.org)
  • This secretory profile, termed the senescence-associated secretory phenotype (SASP), is becoming well established as a negative regulator of tissue and whole-body health. (varbi.com)
  • Reduced insulin receptor expression and altered DNA methylation in fat tissues and blood of women with GDM and offspring. (inventbiotech.com)
  • In group AT, both at 7 and 90 post-operative days, the main healing type was the presence of dense conjunctive tissue exhibiting bundles of collagen fibers disposed in beams permeating the remaining adipose tissue with rare heterotopic bone formation associated to fibrosis and different types of tissue necrosis. (bvsalud.org)
  • Within the limits of this present study, it can be concluded that the adipose tissue grafting did not favor bone neoformation in critical size defects and BMP-2 signaling was not observed. (bvsalud.org)
  • Adipose tissue samples revealed the presence of polychlorinated biphenyl (PCB), Hexachlorobiphenyl (HCB) in addition to pesticides which are found in the milk Average concentrations of organochlorine pesticides in milk matrix were above the Maximum Residue Limits (MRL) of WHO. (scialert.net)
  • Recently I developed a method that is based on the incorporation of 14C from nuclear bomb tests into genomic DNA, which allows for the analysis of cell and tissue turnover in humans. (europa.eu)
  • 4 5 Adipose tissue is an active endocrine organ, heterogeneous with respect to embryonic origin, body distribution and function. (bmj.com)
  • In order to characterize the chicken HSL enzyme, it was purified 3500 fold from a chicken adipose tissue homogenate using pH 5.2 precipitation and anion-exchange chromatography. (lu.se)
  • A porous filter with unique surface property and pre-defined pore size and thickness coupling with a specially formulated detergent-free extraction buffer is employed to rapidly and effectively separate water-oil emulsion derived from adipose tissue homogenate. (inventbiotech.com)
  • The extraction buffer has a lower freezing point than oil in adipose tissues, and the aqueous phase can be quickly separated from the oil phase by passing the tissue homogenate through the filter. (inventbiotech.com)
  • The emergence of single- cell transcriptomics has rapidly transformed our understanding of the diverse array of cell types and cell states residing in adipose tissues and has provided insight into how transcriptional changes in individual cell types contribute to tissue plasticity. (bvsalud.org)