Because of the diversity of L-selectinligands, signals that propagate downstream of L-selectin provide information about the position of the leukocyte within the multistep adhesion cascade (binding, rolling, adhesion, and transmigration). (wikipedia.org)
While L-selectinligands on the apical side of the endothelium have long been characterized as receptors for binding and rolling, glycans enriched on the basolateral side and in the basement membrane likely control quite different signals. (wikipedia.org)
The binding lifetime of L-selectin with apical ligands will be on the order of milliseconds, so in contrast, L-selectin-dependent adhesion in a microenvironment without hydrodynamic shear stress (e.g., within transmigrating pseudopods) will take seconds to minutes. (wikipedia.org)
Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. (wikipedia.org)
Due to a common structural element, the amino-terminal lectin-like domain, the selectins are able to bind to carbohydrate ligands. (biovendor.com)
While the ligands for E- and L-selectins with primary binding activity have not been identified so far, the functionally most important ligand for P-selectin has been identified. (biovendor.com)
The sLeX motif can be presented on cell surfaces on protein (i.e., glycoprotein) and/or lipid (i.e., glycolipid) scaffolds, and these glycoconjugates (known as "E-selectin ligands") program shear-resistant adhesion to endothelial cells. (cancer.gov)
Studies from our laboratory have shown that human HSPCs express a variety of E-selectinligands, and we have also observed that leukemic blasts characteristically express E-selectinligands. (cancer.gov)
We hypothesize that engagement of E-selectinligands on human acute leukemia cells programs efficient BM metastasis and also enables niche lodgment, serving to displace resident HSPCs from their proper growth microenvironment and thereby promoting leukemic cell proliferation. (cancer.gov)
We will identify the pertinent sLeX-bearing glycoconjugates among the different types of human leu- kemia cells, measure the ability of such glycoconjugates to engage E-selectin, and determine how expression of Golgi GTs shape creation of sLeX modifications among the different E-selectinligands. (cancer.gov)
The results of proposed studies will be key to elucidating the glycobiology of leukemogenesis, and should also pro- vide fundamental insights for establishing novel strategies to treat acute leukemias by selectively interrupting sLeX expression and/or E-selectin ligand-mediated processes, and for potential therapy/prognosis stratification schemas based on sLeX levels and/or the expression of distinct E-selectinligands on acute leukemia cells. (cancer.gov)
The nature of the interactions between L-selectin and ligand depends on many circumstances, primarily the location of anatomically defined sites in the high vessel venules (perivascular, extravascular and intravascular). (wikipedia.org)
Leukocyte rolling in postcapillary venules of inflamed tissues is reduced in L-selectin-deficient mice and mice treated with L-selectin blocking antibodies, but the glycoprotein ligand for L-selectin in inflamed venules is unknown. (rupress.org)
Here, we show that L-selectin-dependent rolling after P-selectin blockade is completely absent in P-selectin glycoprotein ligand-1 (PSGL-1) −/− mice or wild-type mice treated with a PSGL-1 blocking monoclonal antibody. (rupress.org)
Different putative ligand structures have been identified for which the selectins show high affinity. (biovendor.com)
The mucin-like glycoprotein PSGL-1 (P-selectin Glycoprotein Ligand-1) has been cloned and sequenced. (biovendor.com)
This information will be integrated with various biochemical approaches including metabolic inhibition of glycosylation and cell surface glycoengineering to custom-modify sLeX display to assess the extent to which sLeX presentation on a specific protein and/or lipid scaffold licenses E-selectin binding among blasts from various subtypes of human acute leukemias, and the impact of the relevant E-selectin receptor/ligand interaction(s) in leukemia cell biology. (cancer.gov)
Integrins are not constitutively active but rather exist in multiple activation states wherein ligand binding stability is related to the activation status of the receptor. (thno.org)
Leukocyte2
L-selectin plays an important role in both the innate and adaptive immune responses by facilitating leukocyte-endothelial cell adhesion events. (wikipedia.org)
To investigate whether leukocyte-expressed PSGL-1 is mediating L-selectin-dependent rolling, we reconstituted lethally irradiated wild-type mice with PSGL-1 −/− bone marrow cells. (rupress.org)
Endothelial cell1
2005). The immortalized microvascular endothelial cell lines express common markers as the platelet endothelial cell adhesion molecule (PECAM1), vascular endothelial cadherin (CDH5), and von Willebrand factor (VWF) (Weksler et?al. (bakingandbakingscience.com)
Molecules2
Over time, these molecules are released through the process of ectodomain shedding and are replaced by newly synthesized L-selectin proteins. (wikipedia.org)
The family of selectins consists of three structurally and functionally related molecules. (biovendor.com)
Determinant2
L-selectin belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups containing a Sialyl LewisX (sLeX) determinant. (wikipedia.org)
CD15s) is the prototypical binding determinant for E- selectin (CD62E), a Ca++-dependent lectin expressed on vascular endothelial cells. (cancer.gov)
Platelets2
Lselectin is constitutively expressed on neutrophils, P-selectin is found on platelets and is stored in Weibel-Palade bodies from where it is transported to the cell surface upon endothelial activation. (biovendor.com)
resting platelets circulate and lower molecular weight vWF multimers are constitutively secreted by the endothelium. (cdc.gov)
Shedding of L-selectin from monocytes occurs during trans-endothelial migration. (wikipedia.org)
blank_start]__________[blank_end] is expressed on endothelial cells but also on leukocytes including neutrophils and monocytes. (goconqr.com)
Homing receptor1
L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. (wikipedia.org)
Soluble2
This soluble isoform of PSGL-1 is still capable of binding to P-selectin, thus representing a competitor for cellular PSGL-1 through which regulation in many physiological and pathological processes can take place. (biovendor.com)
Increased immune infiltrates likely stress the endothelial barrier and lead to increased endothelial activation, evidenced by increased soluble ICAM, P-selectin, and EPCR. (cdc.gov)
In addition to its function in the immune response, L-selectin is expressed on embryonic cells and facilitates the attachment of the blastocyst to the endometrial endothelium during human embryo implantation. (wikipedia.org)
In addition, activating signals may induce the internalization of expressed receptors to selectively down-regulate their intensity. (biomedcentral.com)
Chemokine1
They can be activated by either chemokine engagement or selectin engagement. (goconqr.com)
Kinase1
Several patients with clinical features of chronic myeloid leukemia (CML) have fusion of the TEL (ETV6) gene on 12p13 with ABL on 9q34 and express a chimeric Tel-Abl protein that contains the same portion of the Abl tyrosine kinase fused to Tel, an Ets family transcription factor, rather than Bcr. (shengsci.com)
Proteins1
L-selectin is composed of multiple structural regions: an N-terminus C-type lectin domain, an adjacent epidermal growth factor-like domain, two to the consensus repeat units homologous to those found in C3/C4-binding proteins, an extracellular cleavage site, a short transmembrane domain, and a cytoplasmic tail. (wikipedia.org)
Tissues1
It is constitutively expressed in most tissues and is a key mediator of tumor necrosis factor signaling in the vast majority of cells. (childrensmercy.org)
Cells20
L-selectin is also expressed by lymphoid primed hematopoietic stem cells and may participate in the migration of these stem cells to the primary lymphoid organs. (wikipedia.org)
Most glycoproteins undergo either N- or O-linked glycosylation, and it is very likely that the type of L-selectin glycosylation determines the specific functions of individual cells, but this has not yet been investigated in detail. (wikipedia.org)
L-selectin is expressed on naive T cells and is rapidly shed following T cell priming. (wikipedia.org)
L-selectin expression is re-activated in cytotoxic T cells once they exit the lymph node. (wikipedia.org)
Mature central memory T cells express L-selectin while effector memory cells do not. (wikipedia.org)
L-selectin is also expressed by naive B cells, with the loss of L-selectin distinguishing activated B cells destined to differentiate to antibody-secreting cells L-selectin is expressed on circulating neutrophils and is shed following neutrophil priming. (wikipedia.org)
E-selectin is expressed on endothelial cells. (biovendor.com)
PSGL-1 is expressed on cells of myeloid, lymphoid and dendritic lineage. (biovendor.com)
Presently it is not known which cells, apart from leukocytes, express PSGL-1 and what role PSGL-1 plays on these cells. (biovendor.com)
E-selectin is typically not displayed on resting vascular endothelial cells, and its expression is induced by inflammatory cytokines such as TNF and IL-1. (cancer.gov)
How- ever, conspicuously, E-selectin is constitutively expressed on bone marrow (BM) sinusoidal vessels where it is known to play a key role in mediating migration of circulating cells to BM, a process critical to blood cell recovery following hematopoietic stem cell transplantation (HSCT). (cancer.gov)
In this proposal, using E-selectin binding assays under both static and fluid shear conditions, together with complementary techniques in flow cytometry and western blotting, we will analyze the E-selectin binding activity of leukemia cells isolated from blood and BM of patients with acute leukemias. (cancer.gov)
Selectins are a family of 3 C-type lectins expressed exclusively by bone marrow-derived cells and endothelial cells. (goconqr.com)
and others) [blank_start]__________[blank_end] is predominantly expressed on endothelial cells, but is also present on SMCs. (goconqr.com)
γδ T cells express unique T cell receptor (TCR) γ and TCR δ chains, with structural and functional heterogeneity. (explorationpub.com)
Perfilyeva Y.V., Abdolla N., Ostapchuk Y.O., Tleulieva R.T., Krasnoshtanov V.K., Perfilyeva А.V., Belyaev N.N. Chronic inflammation contributes to tumor growth: Possible role of L-selectin-expressing myeloid-derived suppressor cells (MDSCs) // Inflammation. (org.kz)
Concomitantly, ALP suppressed the IL-1β-induced NF-κB activation and the upregulation of E-selectin expression in glEND.2 cells in vitro . (biomedcentral.com)
constitutively released by murine main cells such as dendritic and transformed cell lines. (exposed-skin-care.net)
Seventy-five percent of these FGF-2-responsive cells were beta tubulin III (TuJ1)-positive neurons, and 25% expressed nestin, a marker for neuroepithelial precursors. (beauty104.com.tw)
BALB/c mice immunized with allogeneic dying melanoma cells expressing revCasp-3 or CpnTCTD demonstrated strong rejection from the allogeneic problem. (treatmentforprostatecancer.info)
Inflammation1
It is constitutively expressed in the skin and may play a role in T-CELL trafficking during cutaneous INFLAMMATION. (lookformedical.com)
Gene1
The human L-selectin gene (sell) is located on the long arm of chromosome 1 (1q24.2), and is arranged in tandem with its family members (in the order: L-, P-, and E-selectin). (wikipedia.org)
Active1
Forced expression of a constitutively active NFATc1 mutant (caNFATc1) has been shown to transform immortalized murine fibroblasts in vitro, while constitutive activation of the NFAT-signaling pathway has been found in a number of human cancers, where it has been shown to contribute towards various aspects of the tumor phenotype. (bvsalud.org)
Molecular weight1
L-selectin varies between cell types, has ranging molecular weight from 65 kDa in lymphocytes to 100 kDa in neutrophils, and is due to cell type-specific glycosylation. (wikipedia.org)
Activation1
The acronym RANTES refers to Regulated on Activation, Normal T Expressed and Secreted. (lookformedical.com)
L-selectin expression is also observed on oocytes and early-stage embryos. (wikipedia.org)
An increase in L-selectin expression is observed when both the blastocyst and cytotrophoblast attach to the endometrium. (wikipedia.org)
L-selectin expression decreases by the 17th week of pregnancy, and remains low or non-existent until term (2017). (wikipedia.org)
Fluorescence-activated cell sorting was applied to determine endothelial E-selectin expression. (biomedcentral.com)
Family1
Metzincins are a ubiquitously expressed family of multi-domain zinc (II)-dependent endopeptidases (Stocker and Bode 1995 ), the members of which include well-known metalloproteases such as the matrix metalloproteases (MMPs) (Nagase and Woessner 1999 ), a disintegrin and metalloproteases (ADAMs) (White 2003 ), the ADAMs with a thrombospondin motif (ADAMTS) (Tang 2001 ), the bacterial serralysins (Nakahama et al. (evelinvahter.com)
Findings1
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. (cdc.gov)