• Polyketide synthases use a similar mechanism and homologous domains to produce secondary metabolite lipids. (wikipedia.org)
  • A common saturated fatty acid found in fats and waxes including olive oil, palm oil, and body lipids. (lookformedical.com)
  • Covalent attachment of LIPIDS and FATTY ACIDS to other compounds and PROTEINS. (lookformedical.com)
  • A group of compounds that are derivatives of octadecanoic acid which is one of the most abundant fatty acids found in animal lipids. (lookformedical.com)
  • Very long-chain fatty acids (VLCFAs) possess more than twenty carbon atoms and are the major components of seed storage oil, wax, and lipids. (nature.com)
  • VLCFAs consist of 20 or more carbon atoms and are the key components of the cell membrane and cuticular lipids in plants 9 . (nature.com)
  • Extraction procedures are optimized to precipitate and remove proteins and nucleic acids while maximizing solubilization of GSLs along with other lipids. (springer.com)
  • Fatty acids (FA) are essential monomeric components that define the structural diversity of lipids and determine their functional properties in the CNS. (encyclopedia.pub)
  • It consists mainly of lipids and is super-duper important for a healthy skin barrier. (keyaseth.in)
  • Ceramides, cholesterol, and fatty acids are essential SC lipids. (keyaseth.in)
  • 16-20 In a recent placebo-controlled clinical trial, researchers found that after only 6 months of lipoic acid supplementation, patients with type II diabetes had reduced fasting blood glucose and lowered levels of oxidized lipids. (lifeextension.com)
  • In these bacteria, the FAS I system produces palmitic acid, and cooperates with the FAS II system to produce a greater diversity of lipid products. (wikipedia.org)
  • The mycobacterial cell envelope consists of a typical plasma membrane, surrounded by a complex cell wall and a lipid-rich outer membrane. (bvsalud.org)
  • Among them, in the ADC cohort, a higher lipid level consisted of three phosphatidylcholine (PC) isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), was associated with a shorter recurrence free period (RFP) and a greater likelihoods of progressed T-factor (≥ pT2) and pleural invasion. (biomedcentral.com)
  • The presence of various types of adipocytes (white, brown, and beige) characterized by the number/size of lipid droplets, mitochondrial density, and thermogenic capacity, further highlights how intricate is the communication of these cell-types with other metabolic tissues to sense nutrients. (frontiersin.org)
  • Fatty acid - One of many molecules that are long chains of lipid carboxylic acid found in fats and oils and in cell membranes as a component of phospholipids and glycolipids. (en-academic.com)
  • The results clearly highlight the influence of the different fatty acids of the Gb 3 sphingolipids on the phase behaviour and the binding properties of Shiga toxin B subunits, even though the membranes were only doped with 5 mol% of the receptor lipid. (springer.com)
  • Furthermore, preferential anionic lipid sequestering by MGS was shown to induce a different fatty acid modeling of E. coli membranes. (lookformedical.com)
  • The intercellular lipid domain comprises equal concentrations of free fatty acids, cholesterol, and ceramides. (keyaseth.in)
  • Desaturation of the fatty acids in glycerolipids is catalyzed by acyl-lipid desaturase (Murata and Wada, 1995). (rehabsociety.org.hk)
  • Cytosolic LCACoA esters are intermediates in lipid synthesis/oxidation and are primarily derived from circulating fatty acids or intramuscular lipid sources such as triglyceride and phospholipid. (diabetesjournals.org)
  • Phytanic acid replaces other fatty acids, including such essential ones as linoleic and arachidonic acids, in lipid moieties of various tissues. (medscape.com)
  • Both BPs and Muddy become TPP-independent through single amino acid substitutions in their tail spike proteins, and M. abscessus mutants resistant to TPP-independent phages reveal additional resistance mechanisms. (bvsalud.org)
  • Proteins from the mutants and from wild-type cells were compared by two-dimensional polyacrylamide gel electrophoresis, and the only observed difference was in the surface antigen V-1. (lookformedical.com)
  • In this review, we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson's disease, with a special emphasis on functional bacterial amyloid proteins, bacterial biosurfactants, endotoxins and short-chain fatty acids. (techscience.com)
  • Fats, proteins, and organic acids also act as respiratory substrates. (gsebsolutions.in)
  • To solve this dilemma, cells must use their own proteins as sources of amino acids in response to the nutritional downshift. (go.jp)
  • palmitic, stearic, or oleic acid, consisting of a long hydrocarbon chain ending in a carboxyl group that bonds to glycerol to form a fat. (en-academic.com)
  • Fatty acid synthase is a multi-enzyme protein that catalyzes fatty acid synthesis. (wikipedia.org)
  • Its main function is to catalyze the synthesis of palmitate (C16:0, a long-chain saturated fatty acid) from acetyl-CoA and malonyl-CoA, in the presence of NADPH. (wikipedia.org)
  • Type II is found in archaea, bacteria and plant plastids, and is characterized by the use of discrete, monofunctional enzymes for fatty acid synthesis. (wikipedia.org)
  • A coenzyme A derivative which plays a key role in the fatty acid synthesis in the cytoplasmic and microsomal systems. (lookformedical.com)
  • The mechanism of FA synthesis consists of a coordinated series of enzymatic reactions that sequentially extend an alkanoic chain by a series of decarboxylative condensation reactions ( Figure 1 ) [ 3 ] . (encyclopedia.pub)
  • Long chain saturated FAs (LCSFA) and unsaturated FAs of ω9 and ω7 can be synthesized from palmitic acid (PA, C16:0) produced by the de novo FA synthesis. (encyclopedia.pub)
  • A non-targeted metabolomics approach was used to reconstruct the metabolic pathways for the synthesis of oxylipins, derived from roughanic, linoleic, α-linolenic, and arachidonic acid in wild type, the oxylipin-deficient mutants of Ppaos1 and Ppaos2 , the mutants of Ppdes being deficient in all fatty acids harboring a Δ 6 -double bond and the C20-fatty acid-deficient mutants of Ppelo . (frontiersin.org)
  • 2001) shows a pleiotropic loss of all ATP synthase subunits, but a specific reduction in the rate of synthesis of subunit IV (Lemaire and Wollman, 1989b). (rehabsociety.org.hk)
  • In mammals, FA elongation depends on a set of enzymes termed elongation of very long-chain fatty acid enzymes (ELOVLs) [ 7 ] . (encyclopedia.pub)
  • Botryococcus braunii is a green algae capable of producing botryococcene using squalene synthase-like (SSL) enzymes. (plu.edu)
  • The enzymes, pyruvic acid decarboxylase, and alcohol dehydrogenase catalyze these reactions. (gsebsolutions.in)
  • For example, in an environment rich in amino acids, cells do not expend energy for making amino acid biosynthetic enzymes. (go.jp)
  • However, if the environment becomes depleted of amino acids (nutritional downshift), cells will be exposed to a lack of both the amino acid biosynthetic enzymes and the amino acids required to make these enzymes. (go.jp)
  • Once amino acid biosynthetic enzymes start to accumulate, the cell is able to produce its own amino acids, and a new growth phase begins. (go.jp)
  • Ceramides comprise long-chain sphingoid bases linked to long-chain free fatty acids via amide bonds. (keyaseth.in)
  • They are composed of long-chain sphingoid bases ( dihydrosphingosine, sphingosine, phytosphingosine or 6-hydroxy sphingosine ), which are linked to long-chain free fatty acids ( non-hydroxy fatty acids, α-hydroxy fatty acids or ester-linked ω-hydroxy fatty acids ) via amide bonds. (keyaseth.in)
  • Mycobacteria grow by polar extension and recent studies showed that cell envelope incorporation of mycolic acids, the major constituent of the cell wall and outer membrane, is coordinated with peptidoglycan biosynthesis at the cell poles. (bvsalud.org)
  • Using fluorescence microscopy approaches, we investigated the subcellular localization of MmpL3 and MmpL10, respectively involved in the export of mycolic acids and TPP, in growing cells and their colocalization with Wag31, a protein playing a critical role in regulating peptidoglycan biosynthesis in mycobacteria. (bvsalud.org)
  • A fatty acid coenzyme derivative which plays a key role in fatty acid oxidation and biosynthesis. (lookformedical.com)
  • FAE ( Fatty Acid Elongation ) like genes take part in the biosynthesis of VLCFAs, growth regulation, and stress responses, and are further comprised of KCS ( Ketoacyl-CoA synthase ) and ELO ( Elongation Defective Elongase ) sub-gene families. (nature.com)
  • Mammalian Wax Biosynthesis II: Expression Cloning of Wax Synthase cDNAs Encoding a Member of the Acyltransferase Enzyme Family'', J Biol Chem. (wikidoc.org)
  • Mitochondrial function was assessed by measuring citrate synthase (CS) and β-hydroxyacyl-CoA dehydrogenase (β-HAD) activities in muscle biopsies. (biomedcentral.com)
  • In the males, a higher citrate synthase activity-a marker of mitochondrial content-was measured at 2 months in the SRCD compared to the MCD group, and this was followed by a decline with age in the SRCD group only. (oroboros.at)
  • 42 carboxylic acids such as citrate and lactate (ATSDR 2001e). (cdc.gov)
  • Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. (wikipedia.org)
  • Then, Acetyl-CoA and malonyl-CoA are coupled to the acyl-carrier protein domain of the rate-limiting enzyme fatty acid synthase (FASN) [ 4 ] . (encyclopedia.pub)
  • Any of a class of aliphatic carboxylic acids, of general formula CHCOOH, that occur combined with glycerol as animal or vegetable oils and fats. (en-academic.com)
  • However, this pathway has only one known product, lipoic acid, which functions as a cofactor for several important mitochondrial multienzyme complexes [ 6 ] . (encyclopedia.pub)
  • For almost two decades, scientists have known that lipoic acid offers an effective solution to mitochondrial dysfunction. (lifeextension.com)
  • Fortunately, you don't need to wait for Big Pharma to develop a synthetic, costly version of lipoic acid in order to combat mitochondrial dysfunction . (lifeextension.com)
  • Lipoic acid increases the abundance of other protective antioxidants while improving mitochondrial function and preventing glycation-induced damage in the kidney and blood vessels of those with diabetes-thus defending against diabetic kidney disease, vision loss, and vascular dysfunction. (lifeextension.com)
  • title=Schneider J, Han WH, Matthew R, Sauve Y, Lemieux H (2020) Age and sex as confounding factors in the relationship between cardiac mitochondrial function and type 2 diabetes in the Nile Grass rat. (oroboros.at)
  • abstract=Our study revisits the role of cardiac mitochondrial adjustments during the progression of type 2 diabetes mellitus (T2DM), while considering age and sex as potential confounding factors. (oroboros.at)
  • A mitochondrial protein consisting of four alpha-subunits and four beta-subunits. (bvsalud.org)
  • Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
  • Here, we establish that the translocation of non-essential trehalose polyphleates (TPP) occurs at different subcellular locations than that of the essential mycolic acids. (bvsalud.org)
  • These results led us to propose a model in which insertion of TPP and mycolic acids into the mycomembrane is spatially uncoupled. (bvsalud.org)
  • In both strains, mycolic acids had fewer double bond numbers in phenol conditions than the glucose condition, and evol40 had significantly shorter mycolic acid chain lengths than the WT strain in phenol conditions. (biomedcentral.com)
  • Community-level differences in functional potential were driven by enrichment of genes associated with carbon and nitrogen fixation in non-disinfected systems and γ-aminobutyrate metabolism in disinfected systems likely associated with the recycling of amino acids. (biomedcentral.com)
  • The growing fatty acid chain is carried between these active sites while attached covalently to the phosphopantetheine prosthetic group of an acyl carrier protein (ACP), and is released by the action of a thioesterase (TE) upon reaching a carbon chain length of 16 (palmitic acid). (wikipedia.org)
  • Salts and esters of the 16-carbon saturated monocarboxylic acid--palmitic acid. (lookformedical.com)
  • The elongation of the chain occurs by repeating the condensation cycle until a 16-carbon palmitic acid (16:0) is generated. (encyclopedia.pub)
  • Next, palmitic acid is further elongated and desaturated to generate complex fatty acids. (encyclopedia.pub)
  • Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. (lookformedical.com)
  • It is an amino acid derivative and an essential cofactor for fatty acid metabolism. (lookformedical.com)
  • Wild-type IDI1 and cholesterol metabolism related serological parameters are normal in all adults. (nature.com)
  • It contains enoyl-CoA hydratase, long-chain-3-hydroxyacyl-CoA dehydrogenase, and acetyl-CoA C-acyltransferase activities and plays an important role in the metabolism of long chain FATTY ACIDS. (bvsalud.org)
  • Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated eight carbon aliphatic structure. (lookformedical.com)
  • any of a class of aliphatic acids, esp. (en-academic.com)
  • In addition, females, but not males, were able to adjust their capacity to oxidize long-chain fatty acid in accordance with the fat content of the diet. (oroboros.at)
  • Grisemycin, salinipeptin, and cypemycin belong to the linaridin class of ribosomally synthesized and posttranslationally modified peptides that contain multiple dehydrobutyrine and D-amino acid residues. (hokudai.ac.jp)
  • The biosynthetic gene clusters of these linaridins lack obvious candidate genes for the dehydratase and epimerase required to introduce dehydrobutyrine and D-amino acid residues, respectively. (hokudai.ac.jp)
  • PKB activation involves phosphorylation of two amino acid residues, Ser473 and Thr308. (justia.com)
  • It differs from most FeS clusters in that two of the amino acid residues that This proton gradient across the thylakoid membrane creates a power, i.e. (rehabsociety.org.hk)
  • Objective: Vanin1 (VNN1) is a pantetheinase that can catalyze the hydrolysis of pantetheine to produce pantothenic acid and cysteamine. (koreascience.kr)
  • The addition of an organic acid radical into a molecule. (lookformedical.com)
  • Triglycerides are a type of fat found in the blood that are made up of three fatty acids and a glycerol molecule. (lookformedical.com)
  • In Escherichia coli , amino acid starvation leads to the accumulation of an unusual molecule, polyphosphate (polyP), a linear polymer of many hundreds of orthophosphate residues. (go.jp)
  • The putative swf operon consists of swfA (type I PKS/FAS), swfB (reductase and sulphotransferase domains) and swfC (radical S-adenosylmethionine, or radical SAM). (cirn-na.com)
  • In this study, 53 KCS genes were identified in B. carinata compared to 32 and 33 KCS genes in B. nigra and B. oleracea respectively, which suggests that polyploidization might has impacted the fatty acid elongation process during Brassica evolution. (nature.com)
  • The current study provides a basis to understand the evolution of both KCS and ELO genes in fatty acid elongation and their role in stress tolerance. (nature.com)
  • In animal cells also, like muscles during exercise, when oxygen is inadequate for cellular respiration pyruvic acid is reduced to lactic acid by lactate dehydrogenase. (gsebsolutions.in)
  • Tocopherols are lipophilic in nature and are found in association with lipoproteins, fat deposits and cellular membranes and protect the polyunsaturated fatty acids from peroxidation reactions. (biomedcentral.com)
  • Nonalcoholic fatty liver disease (NAFLD) is a globally observed metabolic disease with high prevalence both in adults and children. (mdpi.com)
  • 30,31 Lipoic acid has been particularly effective in reducing body weight and BMI in patients taking antipsychotics, which are known for causing weight gain and other metabolic disturbances. (lifeextension.com)
  • Lipoic acid displays striking benefits in components of metabolic syndrome, including inducing weight loss, improving insulin resistance, and lowering blood pressure. (lifeextension.com)
  • Several other promising scientific studies now show that lipoic acid has a beneficial effect on a number of the factors involved in metabolic syndrome: it reduces triglyceride levels, lowers cholesterol, improves fasting plasma glucose, and causes weight loss in rodent models of obesity. (lifeextension.com)
  • The recommended practices address the benefits of using a quality management system approach, factors to consider before introducing new tests, establishment and verification of test performance specifications, the total laboratory testing process (which consists of the preanalytic, analytic, and postanalytic phases), confidentiality of patient information and test results, and personnel qualifications and responsibilities for laboratory testing for inherited metabolic diseases. (cdc.gov)
  • Type I systems utilise a single large, multifunctional polypeptide and are common to both animals and fungi (although the structural arrangement of fungal and animal syntheses differ). (wikipedia.org)
  • Genome-level analyses for a subset of phylogenetically-related microorganisms suggests that disinfection selects for microorganisms capable of using fatty acids, presumably from microbial decay products, via the glyoxylate cycle. (biomedcentral.com)
  • This observation correlates with the experimental determination that when cells lack the ability to repair certain types of oxidative damage as a result of gene knockout, we see an increase in replicative mutation rates and adaptive mutation frequencies when compared to the wild type cell line. (plu.edu)
  • Type III glycogen storage disease (GSD III) is an autosomal recessive disease caused by mutations in the AGL gene, which codes for glycogen debranching enzyme. (medscape.com)
  • Based on the above, it was proposed that adult Refsum disease could be divided into types 1 and 2, depending on which gene is defective. (medscape.com)
  • In humans, the PHYH gene is about 21 kb and consists of 9 exons and 8 introns. (medscape.com)
  • 2 Much of their efforts are aimed at developing a patentable version of a natural compound called lipoic acid . (lifeextension.com)
  • In this report, you will discover how the alpha lipoic acid benefits vital cellular energy while shielding against and possibly reversing the damaging effects of disorders that include diabetes, obesity, cardiovascular disease, neurodegeneration, and cancer. (lifeextension.com)
  • For many years, scientists have researched how lipoic acid affects insulin sensitivity and the severity of diabetes-related disorders, including heart disease, stroke, glaucoma, neuropathic pain, and kidney disease. (lifeextension.com)
  • Significant research into lipoic acid has centered on its benefits in diabetic neuropathy . (lifeextension.com)
  • 11 However, doctors in Germany have used lipoic acid for more than a decade to reduce pain and inflammation in those suffering from diabetic peripheral neuropathy. (lifeextension.com)
  • Over 15 randomized clinical studies have shown that intravenous administration of 300-600 mg of lipoic acid a day greatly improves nerve function and relieves pain symptoms in diabetic neuropathy patients. (lifeextension.com)
  • 14 Lipoic acid is well tolerated and effective for prolonged use at 600 mg per day (equivalent to 300 mg of R-lipoic acid)-a level that noticeably improves muscle function and stops further impairment in movement. (lifeextension.com)
  • In addition to its anti-diabetic effects, lipoic acid has a known ability to help combat obesity. (lifeextension.com)
  • Even without a change in diet or exercise, large doses of lipoic acid ( 800-1,800 mg/day ) have produced moderate weight loss in obese patients. (lifeextension.com)
  • A saturated 14-carbon fatty acid occurring in most animal and vegetable fats, particularly butterfat and coconut, palm, and nutmeg oils. (lookformedical.com)
  • The unsaturated chain of tocotrienol allows an efficient penetration into tissues that have saturated fatty layers such as the brain and liver. (biomedcentral.com)
  • Laboratory results suggestive of GSD III include ketotic hypoglycemia after short fasting, elevated transaminase levels, and elevated fatty acid concentrations. (medscape.com)
  • To obtain fluorescent Gb 3 derivatives, either fatty acid labelled Gb 3 molecules or head group labelled ones were synthesized. (springer.com)
  • A major class of oxylipins are the circular derivatives to which 12-oxo-phytodienoic acid (OPDA) and its metabolite jasmonic acid (JA) belong. (frontiersin.org)
  • Furthermore, we demonstrated that GrmH/GrmL complex accepted salinipeptin and cypemycin precursor peptides, which possess three additional amino acids. (hokudai.ac.jp)
  • A wild type strain was exposed to the selective pressure canavanine for various lengths of time, incubated with an oxidation-specific dye (CellRox Green) and analyzed using fluorescence microscopy. (plu.edu)
  • In fermentation, say by yeast, the incomplete oxidation of glucose is achieved under anaerobic conditions by sets of reactions where pyruvic acid is converted to CO 2 and ethanol. (gsebsolutions.in)
  • In this process, glucose undergoes partial oxidation to form two molecules of pyruvic acid. (gsebsolutions.in)
  • Patients with Refsum disease are unable to degrade phytanic acid because of a deficient activity of phytanoyl-CoA hydroxylase (PhyH), a peroxisomal enzyme catalyzing the first step of phytanic acid alpha-oxidation. (medscape.com)
  • Refsum disease is a recessive disorder characterized by defective peroxisomal alpha-oxidation of phytanic acid. (medscape.com)
  • Activation of the acyl carrier protein (ACP) domain of the SwfA protein to its holo-form by co-expression with Svp is the first functional proof of swf type genes in marine sponges. (cirn-na.com)
  • The neutral type of selection seemed to be predominant in both KCS and ELO genes evolution. (nature.com)
  • Although at least 14 unique GSDs are discussed in the literature, the 4 that cause clinically significant muscle weakness are Pompe disease ( GSD type II , acid maltase deficiency), Cori disease (GSD type IIIa, debranching enzyme deficiency), McArdle disease ( GSD type V , myophosphorylase deficiency), and Tarui disease ( GSD type VII , phosphofructokinase deficiency). (medscape.com)
  • One form, Von Gierke disease ( GSD type Ia , glucose-6-phosphatase deficiency), causes clinically significant end-organ disease with significant morbidity. (medscape.com)
  • [ 6 ] This situation leads to an essential fatty acid deficiency, which is associated with the development of ichthyosis. (medscape.com)
  • We used brain endothelial cells and mouse models of acid sphingomyelinase-deficient Niemann Pick disease (NPD), and postmortem LSD patients' brains, all compared to respective controls. (ibecbarcelona.eu)
  • Because triglyceride is a relatively inert intracellular metabolite, it is likely that it serves more as a marker for other fatty acid-derived metabolites that actually mediate the insulin resistance, such as cytosolic long-chain acyl-CoA (LCACoA). (diabetesjournals.org)
  • Different kinds of microorganisms express different PAMPs, including mainly lipopolysaccharides (LPSs), phosphoteichoic acid, peptidoglycan, and other structures that usually do not exist in the host. (frontiersin.org)
  • Yeast FAS has a highly efficient rigid barrel-like structure with 6 reaction chambers which synthesize fatty acids independently, while the mammalian FAS has an open flexible structure with only two reaction chambers. (wikipedia.org)
  • IDI2 determines isoprene production as only DMAPP sources isoprene and unlike plants, humans lack isoprene synthase and its enzyme homologue. (nature.com)
  • The epithelium of the mouse tongue and soft palate consists of at least three specific epithelial cell populations: basal cells, keratinized cells organized into filiform and fungiform papillae, and taste receptor cells present in tight clusters known as taste buds in the fungiform and circumvallate papillae and soft palate. (researchdataservice.com)
  • While alpha-tocopherol was the first vitamin E isomer to be recognized, eight chemically distinct isomers are now known, consisting of alpha (α), beta (β), gamma (γ) and delta (δ)-tocopherols and α, β, γ and δ-tocotrienols (T3), all of them are referred to as vitamin E. The tocopherols are saturated forms of vitamin E, whereas the tocotrienols are unsaturated and possess an isoprenoid side chain (Table 1 ). (biomedcentral.com)
  • Considering the chemical structure of ceramides, they are highly lipophilic compounds because the ratio of long-chain fatty acids to the hydrophilic head part is high. (keyaseth.in)
  • Refsum disease (RD) is a neurocutaneous syndrome that is characterized biochemically by the accumulation of phytanic acid in plasma and tissues. (medscape.com)
  • Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1). (lookformedical.com)
  • A third study, also in Malaysia, used human subjects with type 2 diabetes. (vitanetonline.com)
  • FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. (lookformedical.com)
  • Carboxylic acid is an organic acid containing the functional group COOH. (en-academic.com)
  • Interestingly, a GSD type 0 also exists, which is due to defective glycogen synthase. (medscape.com)
  • In this work, we aimed to culture and subculture primary alveolar type II cells on extracellular matrix lung-derived hydrogels to assess their suitability for phenotype maintenance. (ibecbarcelona.eu)
  • For M. tuberculosis , M. bovis , and the various cus sequence typing, restriction fragment length polymor- bacillus Calmette-Guérin daughter strains, genome-wide phism, and fi ngerprinting using variable number of tandem microarray analyses have identifi ed large sequence poly- repeats have shown an apparent lack of genetic diversity of morphisms ( 4 , 8 - 10 ). (cdc.gov)