• The probes are incubated with fragmented genomic DNA, after ligation with sequencing-platform specific adapters. (biomedcentral.com)
  • Evolutionary analyses of the genomic sequences of modern-day lentiviruses have suggested a relatively recent date for their emergence, but the failure to identify any endogenous, vertically transmitted examples has meant that their longer term evolutionary history and origin remain unknown. (pnas.org)
  • Prenatal genomic microarray and sequencing in Canadian medical practice: towards consensus. (cdc.gov)
  • The increased use of genomic sequencing in patients with rare neurogenetic diseases has led to more frequent observation of variants previously reported as pathogenic in the pre-genomic era," said Brent L. Fogel, MD, PhD, FAAN, director of the Neurogenetics Clinic at the University of California, Los Angeles (UCLA). (lww.com)
  • Complementary sequences proximal to the 5′ and 3′ termini are predicted to direct cyclization of the (+)RNAs by forming panhandle structures from which short highly conserved terminal sequences protrude as single-stranded tails. (pasteur.fr)
  • Yamashita A , Kawashita N , Kubota-Koketsu R , Inoue Y , Watanabe Y , Ibrahim MS , Highly conserved sequences for human neutralization epitope on hemagglutinin of influenza A viruses H3N2, H1N1, and H5N1: implication for human monoclonal antibody recognition. (cdc.gov)
  • The elucidation of the human genome sequence has made it possible to identify genetic alterations in cancers in unprecedented detail. (sciencemag.org)
  • This communication describes the consensus multi-locus typing scheme established by the Cryptococcal Working Group I (Genotyping of Cryptococcus neoformans and C. gattii) of the International Society for Human and Animal Mycology (ISHAM) using seven unlinked genetic loci for global strain genotyping. (nih.gov)
  • Development of the first consensus genetic map of intermediate wheatgrass gives insight into the genome and tools for molecular breeding. (springer.com)
  • Genotyping-by-sequencing was used to identify markers that fit expected segregation ratios and construct genetic maps for 13 heterogeneous parents of seven full-sib families. (springer.com)
  • CSIRO Entomology 120 Meiers Road Indooroopilly, Qld 4068, Australia) for providing the consensus sequences in the genetic divergence analysis. (thefreedictionary.com)
  • We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. (semanticscholar.org)
  • Microsatellites, or simple sequence repeats, are genetic loci where several nucleotide bases are repeated in tandem. (uga.edu)
  • Relatively recent dates for the emergence of the modern lentiviral subgroups have been suggested, but these estimates are based on extrapolations of genetic distances observed among contemporary viruses, which are likely to be unreliable for evolutionary comparison of distantly related sequences ( 12 - 15 ). (pnas.org)
  • We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. (plos.org)
  • In our study, we employed long read sequencing combined with genetic and optical mapping, and syntenic information to produce a chromosomal level assembly. (plos.org)
  • A study that found multiple minority patients had previously been given positive results for the risk for hypertrophic cardiomyopathy on the basis of genetic mutations subsequently known to be benign elicited discussion among neurogeneticists about the importance of sequencing more diverse populations. (lww.com)
  • Efforts to increase the diversity of sequencing data available for variant analysis are critical for accurate genetic diagnosis. (lww.com)
  • Whole-genome consensus sequence analysis of a South African rotavirus SA11 sample reveals a mixed. (deepdyve.com)
  • Interestingly, for two subregion types, microRNA and predicted exons, the capture process yields higher than expected coverage when compared to whole genome sequencing. (biomedcentral.com)
  • This process identified numerous, highly significant matches within the whole genome shotgun assembled sequence of the European rabbit ( Oryctolagus cuniculus ). (pnas.org)
  • Because the whole genome shotgun rabbit sequences we recovered were typically 2-10 kb in length (thus rarely containing a complete viral sequence), some of these matches probably represent different parts of the same proviral insertion. (pnas.org)
  • This webinar will provide a comparison of several next-generation sequencing (NGS) approaches - including short-read 16S, whole-genome sequencing (WGS), and synthetic long-read sequencing technology - for use in microbiome research studies. (genomeweb.com)
  • The genotype (N2) and sequence of genome segment 8 of RVA/Simian-tc/ZAF/SA11-N2/1958/G3P(2) were identical to that of the bovine rotavirus O agent. (deepdyve.com)
  • Hi microfuge, I am getting the same error and I mapping reads from several genotypes to one heterozygous reference and was hoping to get the consensus files for each genotype. (biostars.org)
  • Does this error mean it is not possible to get the consensus for each genotype individually? (biostars.org)
  • A multicenter study of NS5b hepatitis C virus (HCV) genotype determination involving 12 laboratories demonstrates that any laboratory with expertise in sequencing techniques would be able to provide a reliable HCV genotype for clinical and epidemiological purposes as long as they are provided a consensus reference sequence database. (mysciencework.com)
  • The HCV RNA was isolated and sequenced either to determine the genotype of the isolated virus or to determine the viral quasispecies diversity within an infected individual. (cdc.gov)
  • Hii people, I have paired-end sequence read of my target species which I have mapped to the refe. (biostars.org)
  • These results show that wheat consensus primer sets can be used to isolate orthologous sequences, especially from species that are used for alien gene transfer in wheat. (kuleuven.be)
  • The population structure of L . calcarifer species complex was analyzed by re-sequencing 61 individuals representing various regions across the species' native range. (plos.org)
  • In addition, population structure of the species was analysed based on low-coverage genome sequence information from 61 individuals representing diverse geographic locations stretching from North-Western India across South-East Asia and Australia to Papua New Guinea. (plos.org)
  • Ancient DNA sequences have been retrieved from only a few fossils older than 100,000 years, but such DNA sequences can be tremendously useful in determining how different species are related to each other. (elifesciences.org)
  • These sequences were analysed to determine how straight-tusked elephants are related to the three living elephant species and the extinct mammoth, the DNA sequences for which can be found in public databases. (elifesciences.org)
  • Future research should include obtaining DNA sequences from additional extinct elephant species as well as careful re-evaluation of skeletal measurements for reconstructing elephant evolution. (elifesciences.org)
  • However, a region clearly related to exon 2 was identified in humans from its strong sequence homology to expressed exon 2 in other species. (mad-cow.org)
  • Exon 2 is changing very slowly in evolutionary time, with 6 species averaging 85% identity to a common ancestral consensus sequence some 100 million years back. (mad-cow.org)
  • In contrast, sequence comparison among N-6-adenine and N-4-cytosine Mtases indicated two of the conserved segments [ PMID: 2690010 ], although more conserved segments may be present. (ebi.ac.uk)
  • The predicted secondary structure, and sequence comparison with kinases of known structure, is consistent with uridine kinase having the alpha/beta core nucleotide-binding fold common to many kinases [ PMID: 8951040 ]. (ebi.ac.uk)
  • This webinar will demonstrate how clinical laboratories can develop their own customized targeted next-generation sequencing (NGS)-based solid tumor panels. (genomeweb.com)
  • The general strategy of current character-based methods for an appropriate tree reconstruction from a homologous sequence set relies in the first place on an assumed tree for a given set of taxa. (biomedcentral.com)
  • Understanding the expected variant density of these regions and our ability to sequence them are therefore important considerations for designing future capture experiments. (biomedcentral.com)
  • I downloaded a fasta file with a consensus sequence and a vcf file with the variant for certain i. (biostars.org)
  • In the direction of transcription along the DNA strand, the TATA box has the core DNA sequence 3'-TATAAA-5' or a variant, which is usually followed by three or more adenine (A) bases, specifically [3'-TATAAA(A)AAA-5' on the template strand]. (wikiversity.org)
  • By including the same individual animal from which the M. murinus draft genome was derived, we are able to cross-validate gene copy estimates from Sanger sequencing versus CCS methods. (duhnnae.com)
  • Indeed, 7 most frequent sequences reconstructed by ViSpA from a real HCV dataset are viable (do not contain internal stop codons), and the most frequent sequence was within 1% of the actual open reading frame obtained by cloning and Sanger sequencing. (biomedcentral.com)
  • We present, to our knowledge, the first genome-wide sequence data from eight individuals associated with archaeological remains from farming cultures in the El Portaln cave (Atapuerca, Spain). (scribd.com)
  • Here, the authors generate a SOSIP trimer based on the consensus sequence of group M isolates, determine its structure and exposure of common epitopes, and show immunogenicity in rabbits and non-human primates. (nature.com)
  • Although a consensus sequence was found in case of PSA-C1 clones with high frequency, this corresponds only in two amino acid positions with PSA (amino acids 9-15), and two amino acids were of similar physicochemical characteristics (W vs Y). (thefreedictionary.com)
  • The absence of substitutions, or the presence of only very conservative substitutions (that is, the substitution of amino acids whose side chains have similar biochemical properties) in a particular region of the sequence, suggest that this region has structural or functional importance. (wikipedia.org)
  • The fundamental idea behind all these notations is the matching principle, which assigns a meaning to a sequence of elements of the pattern notation: a sequence of elements of the pattern notation matches a sequence of amino acids if and only if the latter sequence can be partitioned into subsequences in such a way that each pattern element matches the corresponding subsequence in turn. (wikipedia.org)
  • Results: We generated 34,088 high quality circular consensus sequences of two diverse V1R subfamilies here referred to as V1RI and V1RIX from two individuals of Microcebus murinus. (duhnnae.com)
  • The BAC end sequences have been submitted to NCBI dbGSS under the accession numbers KS320706 - KS326261 for the Bam HI library and KS326262 - KS331896 for the Eco RI library. (plos.org)