• Antibody is a protein made by plasma cells in the blood to respond and counteracting a specific antigen. (mrenzyme.com)
  • Antibody titers increased in serum from five chinchillas immunized with a type 6B outer membrane protein complex vaccine. (docksci.com)
  • Since the conjugated polyribosyl ribitol phosphate-OMPC vaccine elicits a protective antibody response in infants as young as 2 months of age (31), it is reasonable to expect that the PCP-OMPC conjugates will have equally good immunogenicities. (docksci.com)
  • Frequently used methods are based on, for example, antigen-antibody systems, nucleic acid hybridization techniques, and protein-ligand systems. (justia.com)
  • Using ELISA, we demonstrated that the recombinant protein specifically interacts with the anti-benzo[a]pyrene monoclonal antibody (mAB B2). (actanaturae.ru)
  • Competitive immunoassay-validated hapten-carrier conjugates BSA-Trimethoprim(TMP) with anti-Hapten antibody. (genemedi.net)
  • The hapten hapten-carrier conjugates BSA-Trimethoprim(TMP) had been validated with our anti-Hapten antibody Anti-Trimethoprim(TMP) mouse monoclonal antibody via competitive ELISA test. (genemedi.net)
  • Product Description google Rabbit anti-Autophagy-related protein 7 (ATG7) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen. (biosensis.com)
  • Product Description Rabbit anti-Autophagy-related protein 7 (ATG7) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen. (biosensis.com)
  • Preparation of Hapten-carrier Immunogen conjugates - antibody modification and conjugation immunotoxin conjugation techniques - liposome conjugated and derivatives- Colloidal - gold- labeled proteins - modification with synthetic polymers. (notesforgeeks.in)
  • Immunogen Description A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. (biosensis.com)
  • Conjugate vaccines in which polysaccharide antigens are covalently linked to carrier proteins belong to the most effective and safest vaccines against bacterial pathogens. (biomedcentral.com)
  • State-of-the art production of conjugate vaccines using chemical methods is a laborious, multi-step process. (biomedcentral.com)
  • In vivo enzymatic coupling using the general glycosylation pathway of Campylobacter jejuni in recombinant Escherichia coli has been suggested as a simpler method for producing conjugate vaccines. (biomedcentral.com)
  • After induction glycoconjugates generally appeared later than unglycosylated carrier protein, suggesting that glycosylation was the rate-limiting step for synthesis of conjugate vaccines in E. coli . (biomedcentral.com)
  • The described methodologies constitute an important step towards cost-effective in vivo production of conjugate vaccines, which in future may be used for combating severe infectious diseases, particularly in developing countries. (biomedcentral.com)
  • In contrast to isolated bacterial polysaccharides, conjugate vaccines induce a long-lasting T-lymphocyte dependent immunological memory [ 4 , 5 ]. (biomedcentral.com)
  • Most notably routine immunization of infants with conjugate vaccines against Haemophilus influenzae type B led to a fast and dramatic drop in respective disease incidents after implementation. (biomedcentral.com)
  • State-of-the art production technologies for conjugate vaccines are complex, multi-step processes (Figure 1 ). (biomedcentral.com)
  • The processes are time-consuming and costly, and often large-scale cultivation of pathogenic bacteria is required for polysaccharide biosynthesis, making conjugate vaccines prohibitively expensive for vaccination campaigns in developing countries. (biomedcentral.com)
  • Current method for the production of conjugate vaccines and in vivo biosynthesis . (biomedcentral.com)
  • Omniose is an early-stage biotechnology company developing polysaccharide conjugate vaccines against serious bacterial threats using its proprietary bioconjugation platform. (omniose.com)
  • Conjugation of the capsular polysaccharides of Streptococcus pneumoniae to protein carriers has introduced a generation of pneumococcal vaccines which may be efficacious in preventing pneumococcal otitis media during infancy. (docksci.com)
  • A sensitive, isotype-specific anti-PCP assay is required to explore further the immunogenicity of the new PCP-OMPC conjugate vaccines and to study the immunopathogenesis of pneumococcal otitis media in the chinchilla model. (docksci.com)
  • Conjugation of the capsular polysaccharides of S. pneumoniae to protein carriers has introduced a second generation of pneumococcal vaccines (30). (docksci.com)
  • These recommendations include information on two vaccines recently licensed for use among infants: Haemophilus b Conjugate Vaccine (PRP-T {ActHIB(TM), OmniHIB(TM)}), manufactured by Pasteur Merieux Vaccins, and TETRAMUNE{TM}, manufactured by Lederle Laboratories/Praxis Biologics. (cdc.gov)
  • Specific characteristics of the four conjugate vaccines available for infants and children vary (e.g., the type of protein carrier, the size of the polysaccharide, and the chemical linkage between the polysaccharide and carrier) ( Table 1 ). (cdc.gov)
  • Current recommendations for universal vaccination of infants require parenteral administration of three different vaccines (diphtheria-tetanus-pertussis {DTP}, Hib conjugate, and hepatitis B) during two or three different visits to a health-care provider. (cdc.gov)
  • and c) provides updated recommendations from the Advisory Committee on Immunization Practices (ACIP) for use of conjugate Hib vaccines and TETRAMUNE{TM} for infants and children. (cdc.gov)
  • Studies have been performed with all four Hib conjugate vaccines to determine immunogenicity in infants 2-6 months of age. (cdc.gov)
  • Type 6B PCP has been conjugated with Neisseria meningitidis group B outer membrane protein complex (OMPC) by a methodology similar to that used in conjugating the polyribosyl ribitol phosphate capsular polysaccharide of Haemophilus influenzae type b to OMPC (25). (docksci.com)
  • Bioconjugation relies on single cells that simultaneously produce a polysaccharide antigen along with an engineered carrier protein and a conjugating enzyme to precisely link them together. (omniose.com)
  • Our platform enables the precise enzymatic attachment of virtually any bacterial polysaccharide (sugar) antigen to engineered carrier proteins within a single E. coli cell. (omniose.com)
  • Enzyme immunoassay for detection of immunoglobulin G (IgG), IgM, and IgA antibodies against type 6B pneumococcal capsular polysaccharide and cell wall C polysaccharide in chinchilla serum. (docksci.com)
  • By using affinity-purified rabbit immunoglobulin G (IgG) anti-chinchilla IgG, IgM, and IgA, we developed a sensitive enzyme immunoassay that is highly specific for IgG, IgM, and IgA antibodies against type 6B PCP (anti-6B) and against C polysaccharide in chinchilla serum. (docksci.com)
  • Conjugation of the PRP polysaccharide with protein carriers confers T-cell- dependent characteristics to the vaccine and substantially enhances the immunologic response to the PRP antigen. (cdc.gov)
  • We describe here a sensitive enzyme immunoassay (EIA) that is highly specific for measuring immunoglobulin G (IgG), IgM, and IgA antibodies against type 6B PCP (anti-6B) in chinchilla serum. (docksci.com)
  • Modification of Amino Acids, Peptides and Proteins - Modification of sugars, polysaccharides and glycoconjugates - modification of nucleic acids and oligonucleotides. (notesforgeeks.in)
  • Properties of common enzymes - Activated enzymes for conjugation - biotinylated enzymes - chemical modification of nucleic acids - biotin labeling of DNA- enzyme conjugation to DNA - Fluorescent of DNA. (notesforgeeks.in)
  • The 414K-MDI conjugation appears to covalently cross- link GSH to albumin via GSH's NH2-terminus, a unique conformation possibly resulting from cyclized mono(GSH)-MDI or asymmetric ( S , N -linked) bis(GSH)-MDI conjugates. (cdc.gov)
  • Peptides derived from sequence of cell surface proteins, such as intercellular adhesion molecule-1 (ICAM-1), LHRH, Bombesin, and LFA-1, have shown potent binding affinity to the target cell surface receptors. (biosyn.com)
  • Moreover, peptides derived from ICAM-1 receptor can be internalized by the leukemic T-cells along with the conjugated moiety offering the promise to selectively treat cancers and autoimmune diseases. (biosyn.com)
  • Arg-Gly-Asp (RGD) peptides,2 poly-Arg peptides), 3,4 proteins (e.g., antibodies,5 transport proteins, and transferrin6), and small molecules (e.g., folate7) have been used to selectively direct drugs to cancer cells with upregulated receptors by forming drug-carrier conjugates (Fig. 1). (biosyn.com)
  • The pathophysiology depends on the underlying condition, of which the most common are Crigler-Najjar syndrome, Gilbert syndrome, hemolytic disorders, and a reduced ability to conjugate bilirubin in infants. (medscape.com)
  • On the basis of findings establishing comparable immunogenicity, a third conjugate vaccine, PRP-T (ActHIB{TM}, OmniHIB{TM}) has now been licensed for use among infants. (cdc.gov)
  • For this purpose, we synthesized carcinogen-protein conjugates and prepared mono- and polyclonal antibodies to benzo[a]pyrene. (actanaturae.ru)
  • Two different periplasmic carrier proteins, AcrA from C. jejuni and a toxoid form of Pseudomonas aeruginosa exotoxin were glycosylated with Shigella O antigens in E. coli . (biomedcentral.com)
  • Coenzymes are non-protein molecules that assisting enzyme reactions by acting as intermediate carriers of electrons. (mrenzyme.com)
  • Thus, there is wide interest in non-radioactive labels, particularly in labels that are observable by spectrophotometric, spin resonance, and luminescence techniques, and reactive materials, such as enzymes that produce such molecules. (justia.com)
  • Joining of two molecules to form a hybrid conjugate with the help of linkers. (notesforgeeks.in)
  • Carrier-linked prodrugs are for drugs with major drawbacks, which are linked to a nontoxic carrier or promoiety through covalent linkage to change or get rid of their undesirable physicochemical properties. (ijpsonline.com)
  • a: oligosaccharyltransferase PglB, b: carrier protein with signal sequence for secretion to the periplasm, c: undecaprenyl-pyrophosphate-linked polysaccharides. (biomedcentral.com)
  • The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)
  • Glycoconjugate synthesis, in particular expression of oligosaccharyltransferase PglB, strongly inhibited growth of E. coli cells after induction, making it necessary to separate biomass growth and recombinant protein expression phases. (biomedcentral.com)
  • As a result, we prepared a recombinant protein composed of the benzo[a]pyrene immunomimetic peptide and pIII-encoding sequences. (actanaturae.ru)
  • Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). (biosensis.com)
  • However, the enzymes used for bioconjugation thus far could only address a limited range of bacterial targets. (omniose.com)
  • In this study we describe the in vivo biosynthesis of two novel conjugate vaccine candidates against Shigella dysenteriae type 1, an important bacterial pathogen causing severe gastro-intestinal disease states mainly in developing countries. (biomedcentral.com)
  • In 1937, Creech and Franks first synthesized conjugates of carcinogens with high-molecular carriers - blood serum proteins. (actanaturae.ru)
  • Serum elevations of ALT activity are rarely observed except in parenchymal liver disease, since ALT is a more liver-specific enzyme than asparate aminotransferase (AST). (cdc.gov)
  • Inhibitor is a molecule that binds to the enzyme to block it activity. (mrenzyme.com)
  • Systematic analyses have revealed that physicochemical properties of the drug-peptide conjugates and their mechanism of receptor-mediated cellular internalization are important controlling factors for developing a successful targeting system. (biosyn.com)
  • The presented data demonstrate that glycosylated proteins can be produced in recombinant E. coli at a larger scale. (biomedcentral.com)
  • The objective of this review is to provide the researchers with a compilation of which carriers would be suitable for prodrug synthesis and what would be their benefits. (ijpsonline.com)
  • The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). (biosensis.com)
  • This review is focused on understanding the factors involved in the development of an effective drug-peptide conjugate with an emphasis on the chemistry and biology of the conjugates. (biosyn.com)
  • Reported results on several promising drug-peptide conjugates have been critically evaluated. (biosyn.com)
  • The obtained benzo[a]pyrene immunomimetic peptide could only simulate chemical carcinogens in the frame of the pIII protein. (actanaturae.ru)
  • Proteomics is the study of the interactions, function, composition, and structures of proteins and their activities in the cell. (mrenzyme.com)
  • Several PCP-protein conjugates have been demonstrated in recently reported studies (1, 5, 12, 13, 32) to have enhanced immunogenicities. (docksci.com)
  • Properties and selection of carriers or promoeities is a very important parameter in successful prodrug design. (ijpsonline.com)
  • Conjugate enzymes are those that require a specific cofactor. (mrenzyme.com)
  • Bioprecursors do not have a promoiety or carrier but yield the active compound upon biotransformation. (ijpsonline.com)
  • These conjugates find use in single molecule DNA sequencing and other applications. (justia.com)
  • Scientific Background FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. (biosensis.com)
  • It was found that efficiency of glycosylation but not carrier protein expression was highly susceptible to the physiological state at induction. (biomedcentral.com)
  • Any of a class of proteins that have carbohydrate groups attached to their polypeptide chain. (mrenzyme.com)
  • MrEnzyme has created a comprehensive database of enzymes, buffers, and reagents from all the major manufactures. (mrenzyme.com)