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  • Toxoid
  • Immunogens Consisting of Oligosaccharides from the Capsule of Haemophilus influenzae Type b Coupled to Diphtheria Toxoid or the Toxin Protein CRM197",J. Clin. (patentgenius.com)
  • Synthesis, Characterization, and Immunological Properties in Mice of Conjugates Composed of Detoxified Lipopolysaccharide of Salmonella paratyphi A Bound to Tetanus Toxoid, with Emphasis on the Role of O Acetyls",Infect.Immun. (patentgenius.com)
  • Purification
  • The stationary phase is first loaded into a column with mobile phase containing a variety of biomolecules from DNA to proteins (depending on the purification experiment). (wikipedia.org)
  • If the protein becomes denatured or if the copper ions are lost in the purification process, the opalescent blue color disappears and the solution becomes a dull grayish color. (wikipedia.org)
  • Immunization
  • Immunization of 2-month-old infants with protein-coupled oligosaccharides derived from the capsule of Haemophilus influenzae type b",J. Pediatrics,107:346-351,(1985). (patentgenius.com)
  • Children at special risk (e.g., sickle cell disease and asplenia) require as full protection as can be achieved using the 7-valent conjugated vaccine, with the more extensive 23-valent vaccine given after the second year of life: In 2001, the Centers for Disease Control (CDC), upon advice from its Advisory Committee on Immunization Practices, recommended the vaccine be administered to every infant and young child in the US. (wikipedia.org)
  • molecules
  • Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. (thermofisher.com)
  • The molecules no longer needed are first washed away with a buffer while the desired proteins are let go in the presence of the eluting solvent (of higher salt concentration). (wikipedia.org)
  • This process creates a competitive interaction between the desired protein and the immobilized stationary molecules, which eventually lets the now highly purified proteins be released. (wikipedia.org)
  • In structural biology, a protein subunit is a single protein molecule that assembles (or "coassembles") with other protein molecules to form a protein complex. (wikipedia.org)
  • amino
  • Antagonists for modifying protein-protein interactions involving certain amino acid sequences within MMP-9 and/or β1 integrins are described. (freepatentsonline.com)
  • Methods for identifying antagonists that modify protein-protein interactions involving certain amino acid sequences within MMP-9 and/or β1 integrins are also described. (freepatentsonline.com)
  • For example, if a short amine-to-amine crosslinker does not successfully crosslink subunits of a dimeric protein, but a slightly longer crosslinker does, one can conclude that the relevant amino acids in the native dimeric structure exist at a distance approximately equal to the length of the longer crosslinker. (thermofisher.com)
  • When SUMO protein precursors are first expressed, they first undergo a maturation step in which the four C-terminal amino acids are removed, revealing a di-glycine motif. (wikipedia.org)
  • Both encode large glycosylated proteins consisting of around 3400 amino acids and a molecular weight of around 390,000 Daltons, excluding the glycosylation. (wikipedia.org)
  • monoclonal
  • We have investigated the potentiation of transferrin [Tfn]-toxin [Tfn-ricin toxin A chain (RTA) and Tfn-So6 saporin toxin] and monoclonal antibody-RTA conjugates by monensin (Mo) and by a human serum albumin (HSA)-monensin conjugate in vitro. (univr.it)
  • immunogens
  • Few others believed this process could work because the accepted belief at the time was that proteins, not sugars, were immunogens. (wikipedia.org)
  • gene
  • Four alternatively spliced transcript variants encoding the same protein have been found for this gene. (wikipedia.org)
  • The UBC9 protein encoded by the UBE2I gene constitutes a core machinery in the cell's sumoylation pathway. (wikipedia.org)
  • The protein UBC9 encoded by the UBE2I gene has been shown to be targeted by multiple viruses, including HIV and HPV. (wikipedia.org)
  • A polypeptide chain has one gene coding for it - meaning that a protein must have one gene for each unique subunit. (wikipedia.org)
  • Solute carrier organic anion transporter family member 4C1 is a protein that in humans is encoded by the SLCO4C1 gene. (wikipedia.org)
  • adjuvants
  • The product is also formulated with the Neisseria meningitidis outer protein P64k and Montanide ISA 51 as adjuvants to potentiate the immune response. (wikipedia.org)
  • toxin
  • HSA-Mo was active in the same concentration range as Mo in potentiating mAb-RTA and Tfn-toxin conjugates reactive with Tfn receptors expressed by different cell lines in monolayer cell cultures. (univr.it)
  • Antibody Responses to Haemophilus influenzae Type b and Diphtheria Toxin Induced by Conjugates of Oligosaccharides of the Type b Capsule with the Nontoxic Protein CRM",Infect. (patentgenius.com)
  • dilution
  • Briefly, maleimide-activated carrier proteins were reacted with a dilution series of L-cysteine and then the amounts of noncoupled cysteine were measured in a microplate assay using Thermo Scientific Ellman's Reagent, also known as DTNB (Part No. 22582 ). (thermofisher.com)
  • interactions
  • The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. (abcam.com)
  • 1. An antagonist that inhibits angiogenesis by modifying protein-protein interactions between matrix metalloproteinase 9 (MMP-9) and a β1-containing integrin, wherein said antagonist comprises an antibody reagent which specifically binds to a polypeptide consisting of the sequence of SEQ ID NO: 1. (freepatentsonline.com)
  • 2. The antagonist of claim 1 wherein the protein-protein interactions cause MMP-9 to bind to the β1-containing integrin. (freepatentsonline.com)
  • 4. The antagonist of claim 1 wherein the protein-protein interactions cause co-localization of matrix metalloproteinase 9 (MMP-9) and a β1-containing integrin on a cell surface or a blood vessel. (freepatentsonline.com)
  • 11. An antagonist that inhibits angiogenesis by modifying protein-protein interactions, wherein said antagonists comprises an antibody reagent which specifically binds to a polypeptide consisting of the sequence of SEQ ID NO: 1. (freepatentsonline.com)
  • Crosslinking reagents are used in a variety of techniques to assist in determining partners and domains of protein interactions, three-dimensional structures of proteins, and molecular associations in cell membranes. (thermofisher.com)
  • The guide also contains a discussion on various applications where crosslinking has been applied, including the powerful label transfer technique for identifying or confirming protein interactions. (thermofisher.com)
  • Protein-protein interactions are the basis for nearly all cellular pathways, and the discovery and characterization of protein interactions is increasingly important in proteomics research. (thermofisher.com)
  • quaternary
  • Experiments with different lengths of otherwise identical crosslinkers can reveal the molecular distance between particular functional groups in the secondary, tertiary or quaternary protein structure. (thermofisher.com)
  • Protein quaternary structure Allostery Cooperativity Monomer Daniel M, Peek GW, Tollefsbol TO (2012). (wikipedia.org)
  • receptor
  • Previous animal studies revealed that vaccination with siderophore receptor proteins protects against UTI. (pnas.org)
  • subunits
  • Some naturally occurring proteins have a relatively small number of subunits and therefore described as oligomeric, for example hemoglobin or DNA polymerase. (wikipedia.org)
  • Others may consist from a very large number of subunits and therefore described as multimeric, for example microtubules and other cytoskeleton proteins. (wikipedia.org)
  • The subunits of a multimeric protein may be identical, homologous or totally dissimilar and dedicated to disparate tasks. (wikipedia.org)
  • reactions
  • This can make it difficult to prepare for crosslinking reactions and to recover conjugate in subsequent clean-up steps. (thermofisher.com)
  • SENP proteases can remove SUMO from sumoylated proteins, freeing it to be used in further sumoylation reactions. (wikipedia.org)
  • Receptor
  • 168 of 444 (37.8%) LPS-induced proteins were significantly reduced with the treatment of RGFP966, which mainly concentrated on Toll-like receptor signaling pathway. (hindawi.com)
  • This interest has been fueled in part by the recent discovery of a family of highly conserved pattern recognition receptor proteins known as Toll-like receptors (TLRs) believed to be involved in innate immunity as receptors for pathogen- associated molecular patterns (PAMPs). (allindianpatents.com)
  • 8. The fusion protein according to claim 1, wherein the Targeting Moiety is an agonist of a receptor present on a nociceptive sensory afferent. (patentsencyclopedia.com)
  • 10. The fusion protein according to claim 1, wherein the Targeting Moiety binds to the ORL 1 receptor. (patentsencyclopedia.com)
  • An endosomal regulatory interaction of a lipid G-protein coupled receptor (GPCR) and IFNAR1 balances effective and detrimental components of immune responses and provides a previously unidentified pathway that contributes to significant and unexpected efficacy in clinical trials in multiple sclerosis, ulcerative colitis, psoriasis, and likely other diseases with aberrant IFN-α signatures. (pnas.org)
  • vaccine
  • NPs may interact with these proteins in a similar way, and the interaction may lead to beneficial outcomes in vaccine delivery. (scirp.org)
  • What is a conjugate vaccine? (icanpost.net)
  • The first glycoconjugate vaccine for use in humans, a Haemophilus influenzae type b (Hib) conjugate, was licensed in the USA in 1987 and shortly thereafter was introduced into the US infant immunization schedule . (icanpost.net)
  • Since its development, a conjugate vaccine in India plays a great role among children by making them immunized against various diseases. (icanpost.net)
  • This modern method has the power to majorly reduce costs and create new IP, allowing developing country manufacturers undertake more conjugate vaccine development projects. (icanpost.net)
  • Pneumococcal conjugate vaccine (PCV) is a pneumococcal vaccine and a conjugate vaccine used to protect infants, young children, and adults against disease caused by the bacterium Streptococcus pneumoniae (the pneumococcus). (wikipedia.org)
  • Prevnar (PCV7) is a heptavalent vaccine, meaning that it contains the cell capsule sugars of seven serotypes of the bacteria S. pneumoniae (4, 6B, 9V, 14, 18C, 19F and 23F), conjugated with diphtheria proteins. (wikipedia.org)
  • Children under the age of two years fail to mount an adequate response to the 23-valent adult vaccine, and so the 7-valent Pneumococcal Conjugated Vaccine (PCV) (e.g. (wikipedia.org)
  • Children at special risk (e.g., sickle cell disease and asplenia) require as full protection as can be achieved using the 7-valent conjugated vaccine, with the more extensive 23-valent vaccine given after the second year of life: In 2001, the Centers for Disease Control (CDC), upon advice from its Advisory Committee on Immunization Practices, recommended the vaccine be administered to every infant and young child in the US. (wikipedia.org)
  • ubiquitination
  • Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell. (wikipedia.org)
  • binds
  • Once conjugated to ubiquitin, the E2 molecule binds one of several ubiquitin ligases or E3s via a structurally conserved binding region. (wikipedia.org)
  • lysine
  • The E3 molecule is responsible for binding the target protein substrate and transferring the ubiquitin from the E2 cysteine to a lysine residue on the target protein. (wikipedia.org)
  • interaction
  • We investigated the interaction of PEGylated and un-PEGylated PLGA NPs with Recombinant Human Mannose-Binding Protein (HMBP) in an effort to understand the effect of surface modification on their binding to the protein. (scirp.org)
  • This process creates a competitive interaction between the desired protein and the immobilized stationary molecules, which eventually lets the now highly purified proteins be released. (wikipedia.org)
  • activates
  • Formation of the methylglyoxal-glutathione conjugate, S-lactoylglutathione, is catalyzed by glyoxalase I, and S-lactoylglutathione activates KefB and KefC. (wikipedia.org)
  • affinity
  • If the molecular weight, hydrophobicity, charge, etc. of a protein is unknown, affinity chromatography can still apply to this situation. (wikipedia.org)
  • buffer
  • These buffers include the bicarbonate buffer system, the phosphate buffer system, and the protein buffer system. (wikipedia.org)
  • The molecules no longer needed are first washed away with a buffer while the desired proteins are let go in the presence of the eluting solvent (of higher salt concentration). (wikipedia.org)
  • biological
  • Noncovalent bonding is critical in maintaining the 3D structure of large molecules, such as proteins and is involved in many biological processes in which large molecules bind specifically but transiently to one another. (wikipedia.org)
  • function
  • The George Fleming Prize recognises Dr Mobasheri's work in water channel proteins, which he found to play an important role in maintaining the function of cartilage cells following physical exercise in horses. (thefreedictionary.com)
  • In addition to pills, capsules and injectable drug carriers (that often have an additional release function), forms of controlled release medicines include gels, implants and devices (e.g. the vaginal ring and contraceptive implant) and transdermal patches. (wikipedia.org)
  • driven
  • Major transport into the blood stream is driven by an electrochemical gradient, specifically through the transport proteins RcGshT and RsGshT. (wikipedia.org)
  • Oxidation of the resulting ring yields the final pyrrole ring C. Finally, these two pieces are stitched together in a dehydration reaction driven forward by the establishment of a conjugated system across all three rings. (wikipedia.org)
  • sequence
  • Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. (wikipedia.org)
  • form
  • However, oxygenation of 10,10-difluoroarachidonic acid to 11-(S )-hydroxyeicosa-5,8,12,14-tetraenoic acid is not consistent with the generation of a carbanion intermediate because it would eliminate fluoride to form a conjugated diene. (wikipedia.org)
  • Biochemical and biotechnological development has allowed novel approaches to this issue, in the form of engineered O2 carriers, widely known as "blood substitutes. (wikipedia.org)
  • domain
  • 4. The fusion protein according claim 1, wherein the translocation domain is the H N domain of a clostridial neurotoxin. (patentsencyclopedia.com)
  • Both the cyclooxygenase and the peroxidase active sites are located in the catalytic domain, which accounts for approximately 80% of the protein. (wikipedia.org)
  • wherein
  • 3. The fusion protein according to claim 1, wherein the non-cytotoxic protease is a clostridial neurotoxin L-chain or an IgA protease. (patentsencyclopedia.com)
  • 5. The fusion protein according to claim 2, wherein the protease cleavage site is a protease cleavage site that is cleaved by a protease selected from Enterokinase, Factor Xa, Tobacco Etch virus (TEV), Thrombin or PreScission. (patentsencyclopedia.com)
  • important
  • Proteins are also important in cell signaling, immune responses, cell adhesion, active transport across membranes, and the cell cycle. (wikipedia.org)
  • These proteins are important for cell survival during exposure to toxic metabolites, possibly because they can release K+, allowing H+ uptake. (wikipedia.org)
  • components
  • Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter for example, the breaking down of glucose to pyruvate, by cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. (wikipedia.org)
  • There is a strong desire to synthesize polymeric conjugates with bioactive components and other drugs. (wikipedia.org)
  • transport
  • These ancillary proteins stimulate transport activity about 10-fold. (wikipedia.org)
  • The identification of these proteins as members of the CPA2 family reveals that monovalent cation transport is required for Bacillus spore formation and germination. (wikipedia.org)
  • structure
  • However, their binding is shown to have different effect on the structure of the protein. (scirp.org)
  • On the other hand the fluorescence emission of the protein increased upon binding to the PLGA-PEG-NH2 indicating conformational changes in the protein structure. (scirp.org)